Research progress in upconversion nanophosphors for biological imaging

We have summarized the research progress on fluorescent sensors responsive to environmental factors, including local viscosity, polarity, temperature, hypoxia and pH.

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The Research Progress of Fluorescent Probes for Detection of Selenols

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.861.315 ◽

2020 ◽

Vol 861 ◽

pp. 315-319

Author(s):

Yan Gao ◽

Li Han ◽

Jing Jing Liu ◽

Xing Gao ◽

Wen He

Keyword(s):

Cancer Research ◽

Fluorescent Probes ◽

Biological Imaging ◽

Research Progress ◽

Disease Treatment ◽

Hydrogen Selenide

Selenols (R-SeH) are similar to thiols , both of them are important reducing substance. Selenocysteine (Sec) and hydrogen selenide (H2Se) are two important selenols. They play a key role in cancer research and disease treatment. Recently, fluorescent probes and nanoprobes for detecting selenols have been developed rapidly because that fluorescent methods can be applied for biological imaging of cells or in vivo. Therefore, it is very important to the detect selenols. In this paper, we summarized the fluorescent probes for detecting the selenols in recent years.

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Research progress on aggregation induced emission probes with tetraphenylethene structure for biological imaging and drug delivery

Scientia Sinica Chimica ◽

10.1360/n032017-00028 ◽

2017 ◽

Vol 47 (9) ◽

pp. 1075-1084

Author(s):

Yufan Ma ◽

Shan Su ◽

Zhuo Wang ◽

Limei Tian

Keyword(s):

Drug Delivery ◽

Biological Imaging ◽

Research Progress ◽

Aggregation Induced Emission

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X-ray Gabor holography

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100139433 ◽

1995 ◽

Vol 53 ◽

pp. 612-613

Author(s):

Steve Lindaas ◽

Chris Jacobsen ◽

Alex Kalinovsky ◽

Malcolm Howells

Keyword(s):

Surface Relief ◽

Biological Imaging ◽

Specimen Preparation ◽

X Rays ◽

X Ray ◽

Water Window ◽

Biological Objects ◽

Transmission Imaging ◽

Atomic Force ◽

Electron Microscopes

Soft x-ray microscopy offers an approach to transmission imaging of wet, micron-thick biological objects at a resolution superior to that of optical microscopes and with less specimen preparation/manipulation than electron microscopes. Gabor holography has unique characteristics which make it particularly well suited for certain investigations: it requires no prefocussing, it is compatible with flash x-ray sources, and it is able to use the whole footprint of multimode sources. Our method serves to refine this technique in anticipation of the development of suitable flash sources (such as x-ray lasers) and to develop cryo capabilities with which to reduce specimen damage. Our primary emphasis has been on biological imaging so we use x-rays in the water window (between the Oxygen-K and Carbon-K absorption edges) with which we record holograms in vacuum or in air.The hologram is recorded on a high resolution recording medium; our work employs the photoresist poly(methylmethacrylate) (PMMA). Following resist “development” (solvent etching), a surface relief pattern is produced which an atomic force microscope is aptly suited to image.

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Complementary Z-Contrast Imaging and Digital Image Processing

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100118394 ◽

1985 ◽

Vol 43 ◽

pp. 304-305

Author(s):

K. N. Colonna ◽

G. Oliphant

Keyword(s):

Image Processing ◽

Heavy Metal ◽

Digital Image Processing ◽

Digital Image ◽

Biological Tissue ◽

Biological Imaging ◽

Tissue Preparation ◽

Contrast Imaging ◽

Imaging Tool ◽

Z Contrast

Harmonious use of Z-contrast imaging and digital image processing as an analytical imaging tool was developed and demonstrated in studying the elemental constitution of human and maturing rabbit spermatozoa. Due to its analog origin (Fig. 1), the Z-contrast image offers information unique to the science of biological imaging. Despite the information and distinct advantages it offers, the potential of Z-contrast imaging is extremely limited without the application of techniques of digital image processing. For the first time in biological imaging, this study demonstrates the tremendous potential involved in the complementary use of Z-contrast imaging and digital image processing.Imaging in the Z-contrast mode is powerful for three distinct reasons, the first of which involves tissue preparation. It affords biologists the opportunity to visualize biological tissue without the use of heavy metal fixatives and stains. For years biologists have used heavy metal components to compensate for the limited electron scattering properties of biological tissue.

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Which Data to Meta-Analyze, and How?

Zeitschrift für Psychologie ◽

10.1027/2151-2604/a000357 ◽

2019 ◽

Vol 227 (1) ◽

pp. 64-82 ◽

Cited By ~ 3

Author(s):

Martin Voracek ◽

Michael Kossmeier ◽

Ulrich S. Tran

Keyword(s):

Empirical Research ◽

Meta Analysis ◽

Curve Analysis ◽

Research Progress ◽

Primary Data ◽

Statistical Procedures ◽

Graphical Displays ◽

Primary Data Analysis ◽

Meta Analyses ◽

Data Analytic

Abstract. Which data to analyze, and how, are fundamental questions of all empirical research. As there are always numerous flexibilities in data-analytic decisions (a “garden of forking paths”), this poses perennial problems to all empirical research. Specification-curve analysis and multiverse analysis have recently been proposed as solutions to these issues. Building on the structural analogies between primary data analysis and meta-analysis, we transform and adapt these approaches to the meta-analytic level, in tandem with combinatorial meta-analysis. We explain the rationale of this idea, suggest descriptive and inferential statistical procedures, as well as graphical displays, provide code for meta-analytic practitioners to generate and use these, and present a fully worked real example from digit ratio (2D:4D) research, totaling 1,592 meta-analytic specifications. Specification-curve and multiverse meta-analysis holds promise to resolve conflicting meta-analyses, contested evidence, controversial empirical literatures, and polarized research, and to mitigate the associated detrimental effects of these phenomena on research progress.

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