scholarly journals Isolation method of Saccharomyces cerevisiae from red blood cells based on optically induced dielectrophoresis technique for rapid detection of the fungal infections

2021 ◽  
Author(s):  
Mingao Du ◽  
Fei Liu ◽  
Xiaoli Luan ◽  
Gongxin Li
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Rapid Detection ◽  
Fungal Infections ◽  
Isolation Method ◽  
Optically Induced

scholarly journals Black and white teas as potential agents to combine with amphotericin B and protect red blood cells from amphotericin B-mediated toxicity

2018 ◽  
Vol 78 (4) ◽  
pp. 673-678 ◽  
Author(s):  
V. M. Oliveira ◽  
N. M. Khalil ◽  
E. Carraro
Keyword(s):  
Side Effects ◽  
Red Blood Cells ◽  
Amphotericin B ◽  
Blood Cells ◽  
Fungal Infections ◽  
Black Tea ◽  
Additive Effects ◽  
Antifungal Effect ◽  
Black And White ◽  
Fungicidal Substance

Abstract Amphotericin B is a fungicidal substance that is treatment of choice for most systemic fungal infections affecting immunocompromised patients. However, severe side effects have limited the utility of this drug. The aim of this study was to evaluate the antifungal effect of the combination of amphotericin B with black tea or white tea and protective of citotoxic effect. The present study shows that white and black teas have additive effects with amphotericin B against some species Candida. In addition, the combination of white and black tea with amphotericin B may reduce the toxicity of amphotericin B to red blood cells. Our results suggest that white and black tea is a potential agent to combine with amphotericin for antifungal efficacy and to reduce the amphotericin dose to lessen side effects.

scholarly journals Rapid and Label-Free Separation of Burkitt's Lymphoma Cells from Red Blood Cells by Optically-Induced Electrokinetics

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e90827 ◽  
Author(s):  
Wenfeng Liang ◽  
Yuliang Zhao ◽  
Lianqing Liu ◽  
Yuechao Wang ◽  
Zaili Dong ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Burkitt’S Lymphoma ◽  
Burkitt's Lymphoma ◽  
Label Free ◽  
Lymphoma Cells ◽  
Burkitt’S Lymphoma Cells ◽  
Optically Induced

Characterization of the self-rotational motion of stored red blood cells by using optically-induced electrokinetics

2016 ◽  
Vol 41 (12) ◽  
pp. 2763 ◽  
Author(s):  
Wenfeng Liang ◽  
Yuechao Wang ◽  
Hemin Zhang ◽  
Lianqing Liu
Keyword(s):  
Red Blood Cells ◽  
Rotational Motion ◽  
Blood Cells ◽  
The Self ◽  
Optically Induced

scholarly journals Use of pectic polysaccharides for cryopreservation of biological objects

2014 ◽  
Vol 66 (3) ◽  
pp. 1025-1033 ◽  
Author(s):  
Т.V. Polezhaeva ◽  
О.О. Zaitseva ◽  
А.N. Khudyakov ◽  
D.S. Laptev ◽  
V.V. Golovchenko ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Red Blood Cells ◽  
Human Blood ◽  
Blood Cells ◽  
Blood Platelets ◽  
Pectic Polysaccharides ◽  
Pectic Polysaccharide ◽  
Biological Objects ◽  
Human Blood Platelets

The protectant activity of pectic polysaccharides derived from various plants was studied on Saccharomyces cerevisiae yeast-like fungi, human blood platelets and leukocytes, and the antihemolytic action of the same compounds was studied on red blood cells. The feasibility of cryopreservation of biological objects in the environment of pectic polysaccharide- containing cryoprotectant solutions was demonstrated.

scholarly journals The Anticandidal and Toxicity Properties of Lampranthus francisci

2015 ◽  
Vol 2015 ◽  
pp. 1-15 ◽  
Author(s):  
Batanai Moyo ◽  
Stanley Mukanganyama
Keyword(s):  
Red Blood Cells ◽  
Peritoneal Cavity ◽  
Blood Cells ◽  
Fungicidal Activity ◽  
Antifungal Agents ◽  
Fungal Infections ◽  
Ethanol Extract ◽  
Aqueous Extracts ◽  
Succulent Plant ◽  
Mouse Cells

Fungal infections have been rising due to the increasing number of immunocompromised patients and intensive use of some antifungal agents. Lampranthus francisci is an ornamental succulent plant. In Zimbabwe, the fresh sap from the leaves is used to treat fungal scalp infections. The activity of L. francisci fresh and dry acetone, ethanol, hydroethanolic, and aqueous extracts against Candida albicans and Candida krusei was determined. Mouse peritoneal cavity cells and sheep red blood cells were used to investigate L. francisci’s toxicity profile. The hydroethanolic extracts were the most effective extracts against C. albicans. The fresh ethanol extract was the most effective extract against C. krusei. The dry acetone extract, dry ethanol extract, and the fresh and dry aqueous extracts promoted the growth of C. krusei. The hydroethanolic extracts caused haemolysis of sheep cells. The hydroethanolic extracts promoted the growth of the mouse peritoneal cavity red blood cells. Both aqueous extracts increased the density of the mouse cells, but only the fresh extract increased the metabolism of the mouse cells. L. francisci has some fungicidal activity and boosts the growth of immune cells, thus validating its use in ethnomedicine. L. francisci extracts are potential leads for the isolation of immune stimulatory compounds.

Hemoglobin crystals of the red blood cells in the human renal cortex: electron microscopic observations of the renal lithiasis

1978 ◽  
Vol 36 (2) ◽  
pp. 480-481
Author(s):  
Kosuke Ueda ◽  
Hiroto Washida ◽  
Nakazo Watari
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Renal Cortex ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Renal Lithiasis ◽  
Electron Microscopic ◽  
Hemoglobin C ◽  
First Case ◽  
Ultrathin Sections

IntroductionHemoglobin crystals in the red blood cells were electronmicroscopically reported by Fawcett in the cat myocardium. In the human, Lessin revealed crystal-containing cells in the periphral blood of hemoglobin C disease patients. We found the hemoglobin crystals and its agglutination in the erythrocytes in the renal cortex of the human renal lithiasis, and these patients had no hematological abnormalities or other diseases out of the renal lithiasis. Hemoglobin crystals in the human erythrocytes were confirmed to be the first case in the kidney.Material and MethodsTen cases of the human renal biopsies were performed on the operations of the seven pyelolithotomies and three ureterolithotomies. The each specimens were primarily fixed in cacodylate buffered 3. 0% glutaraldehyde and post fixed in osmic acid, dehydrated in graded concentrations of ethanol, and then embedded in Epon 812. Ultrathin sections, cut on LKB microtome, were doubly stained with uranyl acetate and lead citrate.

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