scholarly journals Calcium imaging of neural circuits with extended depth-of-field light-sheet microscopy

2016 ◽  
Vol 41 (5) ◽  
pp. 855 ◽  
Author(s):  
Sean Quirin ◽  
Nikita Vladimirov ◽  
Chao-Tsung Yang ◽  
Darcy S. Peterka ◽  
Rafael Yuste ◽  
...  
Keyword(s):  
Calcium Imaging ◽  
Neural Circuits ◽  
Light Sheet ◽  
Depth Of Field ◽  
Light Sheet Microscopy ◽  
Extended Depth Of Field

scholarly journals An Open-Hardware sample mounting solution for inverted light-sheet microscopes with large detection objective lenses

2019 ◽  
Author(s):  
Craig T. Russell ◽  
Eric J. Rees
Keyword(s):  
Thin Sheet ◽  
Light Sheet ◽  
Depth Of Field ◽  
Standard Methods ◽  
Open Hardware ◽  
Light Sheet Microscopy

Implementations of light-sheet microscopes are often incompatible with standard methods of sample mounting. Light-sheet microscopy uses orthogonal illumination and detection to create a thin sheet of light which does not illuminate the sample outside of the depth of field of the detection axis. Typically, this configuration involves a pair of orthogonal objectives which constrains the positioning of a length of coverslips or microscopes in range of the detection objective. We present an open-hardware (1, 2) sample mounting system for light-sheet microscopes using large detection objectives.

scholarly journals Diffuse light-sheet microscopy for stripe-free calcium imaging of neural populations

2018 ◽  
Vol 11 (12) ◽  
pp. e201800088 ◽  
Author(s):  
Michael A. Taylor ◽  
Gilles C. Vanwalleghem ◽  
Itia A. Favre-Bulle ◽  
Ethan K. Scott
Keyword(s):  
Calcium Imaging ◽  
Light Sheet ◽  
Free Calcium ◽  
Diffuse Light ◽  
Light Sheet Microscopy ◽  
Neural Populations

scholarly journals Cortical Column and Whole Brain Imaging of Neural Circuits with Molecular Contrast and Nanoscale Resolution

2018 ◽  
Author(s):  
Ruixuan Gao ◽  
Shoh M. Asano ◽  
Srigokul Upadhyayula ◽  
Pisarev Igor ◽  
Daniel E. Milkie ◽  
...  
Keyword(s):  
Neural Development ◽  
High Speed ◽  
Large Scale ◽  
Neural Circuits ◽  
Light Sheet ◽  
Synaptic Proteins ◽  
Light Sheet Microscopy ◽  
Mouse Cortex ◽  
Molecular Contrast ◽  
The Brain

AbstractOptical and electron microscopy have made tremendous inroads in understanding the complexity of the brain, but the former offers insufficient resolution to reveal subcellular details and the latter lacks the throughput and molecular contrast to visualize specific molecular constituents over mm-scale or larger dimensions. We combined expansion microscopy and lattice light sheet microscopy to image the nanoscale spatial relationships between proteins across the thickness of the mouse cortex or the entire Drosophila brain, including synaptic proteins at dendritic spines, myelination along axons, and presynaptic densities at dopaminergic neurons in every fly neuropil domain. The technology should enable statistically rich, large scale studies of neural development, sexual dimorphism, degree of stereotypy, and structural correlations to behavior or neural activity, all with molecular contrast.One Sentence SummaryCombined expansion and lattice light sheet microscopy enables high speed, nanoscale molecular imaging of neural circuits over large volumes.

scholarly journals Rapid reconstruction of neural circuits using tissue expansion and lattice light sheet microscopy

2021 ◽  
Author(s):  
Joshua L Lillvis ◽  
Hideo Otsuna ◽  
Xiaoyu Ding ◽  
Igor Pisarev ◽  
Takashi Kawase ◽  
...  
Keyword(s):  
Neural Circuits ◽  
Structural Connectivity ◽  
Tissue Expansion ◽  
Light Sheet ◽  
Structure And Function ◽  
Analysis Pipeline ◽  
Light Sheet Microscopy ◽  
Reference Specimens ◽  
Molecular Contrast ◽  
And Function

Electron microscopy (EM) allows for the reconstruction of dense neuronal connectomes but suffers from low throughput, limiting its application to small numbers of reference specimens. We developed a protocol and analysis pipeline using tissue expansion and lattice light-sheet microscopy (ExLLSM) to rapidly reconstruct selected circuits across many samples with single synapse resolution and molecular contrast. We validate this approach in Drosophila, demonstrating that it yields synaptic counts similar to those obtained by EM, can be used to compare counts across sex and experience, and to correlate structural connectivity with functional connectivity. This approach fills a critical methodological gap in studying variability in the structure and function of neural circuits across individuals within and between species.

scholarly journals Achromatic terahertz Airy beam generation with dielectric metasurfaces

Nanophotonics ◽  
2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Qingqing Cheng ◽  
Juncheng Wang ◽  
Ling Ma ◽  
Zhixiong Shen ◽  
Jing Zhang ◽  
...  
Keyword(s):  
Biomedical Imaging ◽  
Frequency Dispersion ◽  
Light Sheet ◽  
Photonic Devices ◽  
Self Healing ◽  
Beam Focusing ◽  
Light Sheet Microscopy ◽  
Airy Beam ◽  
Potential Applications ◽  
Airy Beams

AbstractAiry beams exhibit intriguing properties such as nonspreading, self-bending, and self-healing and have attracted considerable recent interest because of their many potential applications in photonics, such as to beam focusing, light-sheet microscopy, and biomedical imaging. However, previous approaches to generate Airy beams using photonic structures have suffered from severe chromatic problems arising from strong frequency dispersion of the scatterers. Here, we design and fabricate a metasurface composed of silicon posts for the frequency range 0.4–0.8 THz in transmission mode, and we experimentally demonstrate achromatic Airy beams exhibiting autofocusing properties. We further show numerically that a generated achromatic Airy-beam-based metalens exhibits self-healing properties that are immune to scattering by particles and that it also possesses a larger depth of focus than a traditional metalens. Our results pave the way to the realization of flat photonic devices for applications to noninvasive biomedical imaging and light-sheet microscopy, and we provide a numerical demonstration of a device protocol.

scholarly journals Single-Molecule Light-Sheet Microscopy with Local Nanopipette Delivery

2021 ◽  
Vol 93 (8) ◽  
pp. 4092-4099
Author(s):  
Bing Li ◽  
Aleks Ponjavic ◽  
Wei-Hsin Chen ◽  
Lee Hopkins ◽  
Craig Hughes ◽  
...  
Keyword(s):  
Single Molecule ◽  
Light Sheet ◽  
Light Sheet Microscopy

scholarly journals Effect of captopril on post-infarction remodelling visualized by light sheet microscopy and echocardiography

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Urmas Roostalu ◽  
Louise Thisted ◽  
Jacob Lercke Skytte ◽  
Casper Gravesen Salinas ◽  
Philip Juhl Pedersen ◽  
...  
Keyword(s):  
Morphological Changes ◽  
Light Sheet ◽  
Automated Image Analysis ◽  
Border Zone ◽  
Vascular Density ◽  
Mouse Heart ◽  
Imaging Method ◽  
Light Sheet Microscopy ◽  
Post Surgery ◽  
Captopril Treatment

AbstractAngiotensin converting enzyme inhibitors, among them captopril, improve survival following myocardial infarction (MI). The mechanisms of captopril action remain inadequately understood due to its diverse effects on multiple signalling pathways at different time periods following MI. Here we aimed to establish the role of captopril in late-stage post-MI remodelling. Left anterior descending artery (LAD) ligation or sham surgery was carried out in male C57BL/6J mice. Seven days post-surgery LAD ligated mice were allocated to daily vehicle or captopril treatment continued over four weeks. To provide comprehensive characterization of the changes in mouse heart following MI a 3D light sheet imaging method was established together with automated image analysis workflow. The combination of echocardiography and light sheet imaging enabled to assess cardiac function and the underlying morphological changes. We show that delayed captopril treatment does not affect infarct size but prevents left ventricle dilation and hypertrophy, resulting in improved ejection fraction. Quantification of lectin perfused blood vessels showed improved vascular density in the infarct border zone in captopril treated mice in comparison to vehicle dosed control mice. These results validate the applicability of combined echocardiographic and light sheet assessment of drug mode of action in preclinical cardiovascular research.

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