scholarly journals Simulation of Astrocytic Calcium Dynamics in Lattice Light Sheet Microscopy Images

2021 ◽  
Author(s):  
Anais Badoual ◽  
Misa Arizono ◽  
Audrey Denizot ◽  
Mathieu Ducros ◽  
Hugues Berry ◽  
...  
Keyword(s):  
Light Sheet ◽  
Calcium Dynamics ◽  
Light Sheet Microscopy ◽  
Microscopy Images

scholarly journals Cell morphological motif detector for high-resolution 3D microscopy images

2018 ◽  
Author(s):  
Meghan K. Driscoll ◽  
Erik S. Welf ◽  
Kevin M. Dean ◽  
Reto Fiolka ◽  
Gaudenz Danuser
Keyword(s):  
Intracellular Signaling ◽  
Molecular Mechanisms ◽  
Automated Analysis ◽  
Light Sheet ◽  
Cell Types ◽  
Light Sheet Microscopy ◽  
Cytoskeletal Dynamics ◽  
Computational Workflow ◽  
Analytical Tools ◽  
Microscopy Images

AbstractRecent advances in light-sheet microscopy enable imaging of cell morphology and signaling with unprecedented detail. However, the analytical tools to systematically measure and visualize the intricate relations between cell morphodynamics, intracellular signaling, and cytoskeletal dynamics have been largely missing. Here, we introduce a set of computer vision and graphics methods to dissect molecular mechanisms underlying 3D cell morphogenesis and to test whether morphogenesis itself affects intracellular signaling. We demonstrate a machine learning based generic morphological motif detector that automatically finds lamellipodia, filopodia, and blebs on various cell types. Combining motif detection with molecular localization, we measure the differential association of PIP2 and KrasV12 with blebs. Both signals associate with bleb edges, as expected for membrane-localized proteins, but only PIP2 is enhanced on blebs. This suggests that local morphological cues differentially organize and activate sub-cellular signaling processes. Overall, our computational workflow enables the objective, automated analysis of the 3D coupling of morphodynamics with cytoskeletal dynamics and intracellular signaling.

scholarly journals Large-scale automated identification of mouse brain cells in confocal light sheet microscopy images

2014 ◽  
Vol 30 (17) ◽  
pp. i587-i593 ◽  
Author(s):  
Paolo Frasconi ◽  
Ludovico Silvestri ◽  
Paolo Soda ◽  
Roberto Cortini ◽  
Francesco S. Pavone ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Large Scale ◽  
Light Sheet ◽  
Brain Cells ◽  
Automated Identification ◽  
Light Sheet Microscopy ◽  
Microscopy Images

Restoration of Uneven Illumination in Light Sheet Microscopy Images

2011 ◽  
Vol 17 (4) ◽  
pp. 607-613 ◽  
Author(s):  
Mohammad Shorif Uddin ◽  
Hwee Kuan Lee ◽  
Stephan Preibisch ◽  
Pavel Tomancak
Keyword(s):  
Light Microscopy ◽  
Light Absorption ◽  
Incident Light ◽  
Light Sheet ◽  
Theory Approach ◽  
Light Path ◽  
Light Sheet Microscopy ◽  
The Media ◽  
Microscopy Images ◽  
Degradation Problem

AbstractLight microscopy images suffer from poor contrast due to light absorption and scattering by the media. The resulting decay in contrast varies exponentially across the image along the incident light path. Classical space invariant deconvolution approaches, while very effective in deblurring, are not designed for the restoration of uneven illumination in microscopy images. In this article, we present a modified radiative transfer theory approach to solve the contrast degradation problem of light sheet microscopy (LSM) images. We confirmed the effectiveness of our approach through simulation as well as real LSM images.

scholarly journals A real-time compression library for microscopy images

2017 ◽  
Author(s):  
Bálint Balázs ◽  
Joran Deschamps ◽  
Marvin Albert ◽  
Jonas Ries ◽  
Lars Hufnagel
Keyword(s):  
Real Time ◽  
Single Molecule ◽  
Imaging Techniques ◽  
Intrinsic Noise ◽  
Light Sheet ◽  
Localization Microscopy ◽  
Time Compression ◽  
Light Sheet Microscopy ◽  
Microscopy Images ◽  
Single Molecule Localization Microscopy

AbstractFluorescence imaging techniques such as single molecule localization microscopy, high-content screening and light-sheet microscopy are producing ever-larger datasets, which poses increasing challenges in data handling and data sharing. Here, we introduce a real-time compression library that allows for very fast (beyond 1 GB/s) compression and de-compression of microscopy datasets during acquisition. In addition to an efficient lossless mode, our algorithm also includes a lossy option, which limits pixel deviations to the intrinsic noise level of the image and yields compression ratio of up to 100-fold. We present a detailed performance analysis of the different compression modes for various biological samples and imaging modalities.

scholarly journals Achromatic terahertz Airy beam generation with dielectric metasurfaces

Nanophotonics ◽  
2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Qingqing Cheng ◽  
Juncheng Wang ◽  
Ling Ma ◽  
Zhixiong Shen ◽  
Jing Zhang ◽  
...  
Keyword(s):  
Biomedical Imaging ◽  
Frequency Dispersion ◽  
Light Sheet ◽  
Photonic Devices ◽  
Self Healing ◽  
Beam Focusing ◽  
Light Sheet Microscopy ◽  
Airy Beam ◽  
Potential Applications ◽  
Airy Beams

AbstractAiry beams exhibit intriguing properties such as nonspreading, self-bending, and self-healing and have attracted considerable recent interest because of their many potential applications in photonics, such as to beam focusing, light-sheet microscopy, and biomedical imaging. However, previous approaches to generate Airy beams using photonic structures have suffered from severe chromatic problems arising from strong frequency dispersion of the scatterers. Here, we design and fabricate a metasurface composed of silicon posts for the frequency range 0.4–0.8 THz in transmission mode, and we experimentally demonstrate achromatic Airy beams exhibiting autofocusing properties. We further show numerically that a generated achromatic Airy-beam-based metalens exhibits self-healing properties that are immune to scattering by particles and that it also possesses a larger depth of focus than a traditional metalens. Our results pave the way to the realization of flat photonic devices for applications to noninvasive biomedical imaging and light-sheet microscopy, and we provide a numerical demonstration of a device protocol.

scholarly journals Single-Molecule Light-Sheet Microscopy with Local Nanopipette Delivery

2021 ◽  
Vol 93 (8) ◽  
pp. 4092-4099
Author(s):  
Bing Li ◽  
Aleks Ponjavic ◽  
Wei-Hsin Chen ◽  
Lee Hopkins ◽  
Craig Hughes ◽  
...  
Keyword(s):  
Single Molecule ◽  
Light Sheet ◽  
Light Sheet Microscopy

scholarly journals Effect of captopril on post-infarction remodelling visualized by light sheet microscopy and echocardiography

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Urmas Roostalu ◽  
Louise Thisted ◽  
Jacob Lercke Skytte ◽  
Casper Gravesen Salinas ◽  
Philip Juhl Pedersen ◽  
...  
Keyword(s):  
Morphological Changes ◽  
Light Sheet ◽  
Automated Image Analysis ◽  
Border Zone ◽  
Vascular Density ◽  
Mouse Heart ◽  
Imaging Method ◽  
Light Sheet Microscopy ◽  
Post Surgery ◽  
Captopril Treatment

AbstractAngiotensin converting enzyme inhibitors, among them captopril, improve survival following myocardial infarction (MI). The mechanisms of captopril action remain inadequately understood due to its diverse effects on multiple signalling pathways at different time periods following MI. Here we aimed to establish the role of captopril in late-stage post-MI remodelling. Left anterior descending artery (LAD) ligation or sham surgery was carried out in male C57BL/6J mice. Seven days post-surgery LAD ligated mice were allocated to daily vehicle or captopril treatment continued over four weeks. To provide comprehensive characterization of the changes in mouse heart following MI a 3D light sheet imaging method was established together with automated image analysis workflow. The combination of echocardiography and light sheet imaging enabled to assess cardiac function and the underlying morphological changes. We show that delayed captopril treatment does not affect infarct size but prevents left ventricle dilation and hypertrophy, resulting in improved ejection fraction. Quantification of lectin perfused blood vessels showed improved vascular density in the infarct border zone in captopril treated mice in comparison to vehicle dosed control mice. These results validate the applicability of combined echocardiographic and light sheet assessment of drug mode of action in preclinical cardiovascular research.

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