Determination of Chromium in Human Lung Fibroblast Cells Using a Large Bore—Direct Injection High-Efficiency Nebulizer with Inductively Coupled Plasma Mass Spectrometry

A novel method for the determination of chromium in suspensions of human lung fibroblast cells is described by using a large bore–direct injection high efficiency nebulizer (LB-DIHEN) with micro-scale flow injection analysis and inductively coupled plasma mass spectrometric detection. Chromium(VI)-treated cells were first counted and then suspended in a phosphate buffer saline solution. With the use of the method of standard additions, the relative concentration of Cr in ∼ 100 HLF cells/peak was determined at m/z = 50. Because the cells tend to clump and can yield inhomogeneities in the total number analyzed, Mg was used as an internal standard to compensate for the total cell mass. The level of Cr in HLF cells grown in a medium of 100 μM Na2CrO4 for two hours is on the order of 180 fg Cr/cell after correction for the number of cells in each injection.

Download Full-text

Determination of Depleted Uranium in Urine via Isotope Ratio Measurements Using Large-Bore Direct Injection High Efficiency Nebulizer—Inductively Coupled Plasma Mass Spectrometry

Applied Spectroscopy ◽

10.1366/0003702041959325 ◽

2004 ◽

Vol 58 (9) ◽

pp. 1044-1050 ◽

Cited By ~ 19

Author(s):

Craig S. Westphal ◽

John A. McLean ◽

Shelly J. Hakspiel ◽

William E. Jackson ◽

David E. McClain ◽

...

Keyword(s):

Mass Spectrometry ◽

Inductively Coupled Plasma ◽

Isotope Ratio ◽

High Efficiency ◽

Direct Injection ◽

Depleted Uranium ◽

Inductively Coupled ◽

Plasma Mass Spectrometry ◽

Isotope Ratio Measurements

Download Full-text

Determination of Memory-Prone Elements Using Direct Injection High Efficiency Nebulizer Inductively Coupled Plasma Mass Spectrometry

Applied Spectroscopy ◽

10.1366/000370202760249738 ◽

2002 ◽

Vol 56 (8) ◽

pp. 1006-1012 ◽

Cited By ~ 15

Author(s):

Su-Ann E. O'Brien ◽

John A. McLean ◽

Billy W. Acon ◽

Barbara J. Eshelman ◽

William F. Bauer ◽

...

Keyword(s):

Mass Spectrometry ◽

Inductively Coupled Plasma ◽

High Efficiency ◽

Direct Injection ◽

Dead Volume ◽

Spray Chamber ◽

Inductively Coupled ◽

Freeze Dried ◽

Plasma Mass Spectrometry

A direct injection high efficiency nebulizer (DIHEN) and a large-bore DIHEN (LB-DIHEN) are investigated for the determination of difficult-to-analyze and memory-prone elements (mercury, iodine, and boron). The determination of such elements is problematic using conventional sample introduction systems incorporating spray chambers and/or desolvation devices owing to their large surface area and hence long wash-out characteristics. With the DIHEN or LB-DIHEN, the spray chamber is eliminated and the dead volume is reduced to <10 μL, thus reducing memory effects. In comparison with the crossflow-spray chamber combination, wash-in and wash-out times are reduced to less than 10 s when a DIHEN or LB-DIHEN is used for mercury, iodine, and boron at a concentration of 100 ng/mL. The accuracy of the DIHEN-ICPMS (inductively coupled plasma mass spectrometry) technique is evaluated in the analysis of the National Institute of Standards and Technology standard reference material (NIST SRM) 2670 (Toxic Metals in Freeze-Dried Urine) and NIST SRM 8414 (Bovine Muscle Powder). Further, the effectiveness of the DIHEN-ICPMS technique is examined in the determination of mercury and iodine in an alternative remedy medicine (seahorse genital tonic pills) and boron in rodent liver samples as part of a pharmacokinetic screening study for boron neutron capture therapy.

Download Full-text