Spectrochemical Determination of Nanogram Amounts of Cobalt in Blood Using the Copper-Spark Method

1968 ◽  
Vol 22 (3) ◽  
pp. 183-187 ◽  
Author(s):  
A. M. Kabiel ◽  
Z. G. Hanna ◽  
Y. S. Youssif
Keyword(s):  
Trace Elements ◽  
Ion Exchange ◽  
Human Blood ◽  
Limit Of Detection ◽  
Minor Constituent ◽  
Cobalt Ions ◽  
Concentration Method

The superiority of spectrographic methods in the estimation of cobalt, the minor constituent in human blood, is pointed out. The main advantages are speed, sensitivity, and certainty in the identification of the trace elements being determined. The analysis is carried out using an ion-exchange concentration method to separate nanograms of cobalt ions from the interfering extraneous elements, followed by a copper spark method which proved to be valuable in reducing the limit of detection. Illustrated examples are given of the kinds of work to which the method is applied.

scholarly journals Determination of trace elements in copper by ion-exchange separation using EDTA as a matrix masking reagent

2003 ◽  
Vol 52 (11) ◽  
pp. 1025-1028
Author(s):  
Hitoshi YAMAGUCHI ◽  
Shinji ITOH ◽  
Shin’ichi HASEGAWA ◽  
Kunikazu IDE ◽  
Takeshi KOBAYASHI
Keyword(s):  
Trace Elements ◽  
Ion Exchange ◽  
Ion Exchange Separation

scholarly journals Validation of the TSH IRMA Kit for Determination of the TSH Levels in Human Blood Serum

2018 ◽  
Vol 3 (3) ◽  
pp. 126
Author(s):  
Gina Mondrida ◽  
Sutari Sutari ◽  
Triningsih Triningsih ◽  
Sri Setyowati ◽  
V. Yulianti S ◽  
...  
Keyword(s):  
Blood Serum ◽  
Human Blood ◽  
Limit Of Detection ◽  
Specific Binding ◽  
Thyroid Stimulating Hormone ◽  
The Body ◽  
Human Blood Serum ◽  
Detection Sensitivity ◽  
Assay Precision

<p>TSH IRMA kit is a kit used for the determination of TSH (Thyroid Stimulating Hormone) levels in human blood serum. Thyroid hormone is a hormone that our bodies need for growth of the brain, bone and other tissues and regulate the metabolism in the body.<strong> </strong>TSH normal range for adult is in the range of 0.4-4.5 mIU/L, whereas for baby is about 3.0-18.0 mIU/L. Thyroid would affect the quality of optimal growth of children if disturbed. Therefore, TSH assay in the blood needs to be determined to know whether the function of the thyroid gland works normally or not. Detection of TSH in blood can be performed by Immunoradiometricassay (IRMA) method. IRMA method is one of the immunoassay techniques based on immunological reactions (antigen-antibody binding) using radionuclide <sup>125</sup>I as a tracer, that sample in small quantity can be detected.<em> </em> IRMA method was developed locally by replacing TSH IRMA kit which is costly since imported from commercial companies. Center for Radioisotope and Radiopharmaceutical Technology (PTRR) BATAN has successfully developed the TSH IRMA kit that can be used to determine the levels of TSH in human blood. TSH IRMA kit must be validated to know the limit of detection, sensitivity, accuracy, precision and the assay parameters, such as Non-Specific Binding (NSB) and Maximum Binding (MB). Validation of TSH IRMA kit had been carried out resulting in the limit of detection of 0.115 ng/mL, accuracy with a recovery of 93.6-108.0 %, intra-assay precision (% CV) QC L = 1.9848, QC M = 3.6360 % and QC H = 2.2085 % while the inter-assay precision (% CV) QC L = 11.0055, QC M = 5.6768 %  and  QC H = 5.4181 %.  It was concluded that this TSH IRMA kit showed good performance based on the % NSB and % B/T of 0.68 and 34.64 %, respectively.</p>

scholarly journals Determination of homocysteine by lc-ms-ms with atmospheric pressure chemical ionization

2014 ◽  
Vol 60 (2) ◽  
pp. 235-245
Author(s):  
I.I. Miroshnichenko ◽  
A.I. Platova ◽  
T.P. Safarova ◽  
O.B. Yakovleva
Keyword(s):  
Human Blood ◽  
Atmospheric Pressure ◽  
Chemical Ionization ◽  
Limit Of Detection ◽  
Atmospheric Pressure Chemical Ionization ◽  
Coefficient Of Determination ◽  
Ionization Mass ◽  
Pressure Chemical Ionization ◽  
Pressure Chemical

Homocysteine (Hcy) is an intermediate of methionine metabolism. High plasma Hcy concentrations are an independent risk factor for stroke, peripheral vascular disease, deep venous thrombosis, coronary disease, and cognitive deficiency. Apparently, it is a great importance to measure Hcy levels in human blood. A new method for the quantification of Hcy by means of reversed-phase LC/atmospheric pressure chemical ionization mass spectrometry has been developed. The MRM ion transition, m/z 136.0 ® 90.0 was used for Hcy quantification. The limit of detection was 0.4 mM, quantification was performed from 1 mM to 40 mM with coefficient of determination of R2=0,997. The method was applied successfully to Hcy determination in human blood.

scholarly journals Determination of trace elements in chalcopyrite (CuFeS2) by k0-instrumental neutron activation analysis after matrix elements removal

2008 ◽  
Vol 27 (2) ◽  
pp. 141 ◽  
Author(s):  
Milena Taseska ◽  
Petre Makreski ◽  
Vekoslava Stibilj ◽  
Radojko Jaćimović ◽  
Trajče Stafilov ◽  
...  
Keyword(s):  
Trace Elements ◽  
Neutron Activation Analysis ◽  
Instrumental Neutron Activation Analysis ◽  
Activation Analysis ◽  
Neutron Activation ◽  
Limit Of Detection ◽  
Extraction Procedure ◽  
Matrix Elements ◽  
Instrumental Neutron Activation

A method for trace elements determination in chalcopyrite, CuFeS2, by k0-instrumental neutron activation analysis (k0-INAA) was proposed. To avoid the interference of iron and copper as matrix elements, methods for their separation were applied. The copper interferences were eliminated by applying an electrolytic separation method. To eliminate iron interferences, a liquid-liquid extraction procedure by diisopropyl ether (DIPE) in hydrochloric acid solution and determination of trace elements in the aqueous phase were undertaken. The k0-INAA method was applied to determine the content of the investigated elements, making it possible to follow the distribution of even fifty elements (with intermediate/medium and long half-lived radionuclides) in the studied mineral. The important advantage of the proposed method is the possibility to determine the content of several trace elements after Fe and Cu separation. Additionally, the elimination of the matrix elements lowered the limit of detection for some trace elements in the water phase compared to their corresponding values determined by k0-INAA in the powder sample.

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