Correction: Soil Bacterial Diversity Screening Using Single 16S rRNA Gene V Regions Coupled with Multi-Million Read Generating Sequencing Technologies

ABSTRACT Here, we present a 16S rRNA gene amplicon sequence data set and profiles demonstrating the bacterial diversity of baby and adult elephants from four different geographical locations in Thailand. The dominant phyla among baby and adult elephants were Bacteroidetes, Firmicutes, Proteobacteria, Kiritimatiellaeota, Euryarchaeota, and Tenericutes.

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A converging subset of soil bacterial taxa is permissive to the IncP-1 plasmid pKJK5 across a range of soil copper contamination

FEMS Microbiology Ecology ◽

10.1093/femsec/fiaa200 ◽

2020 ◽

Vol 96 (11) ◽

Author(s):

Jianxiao Song ◽

Uli Klümper ◽

Leise Riber ◽

Arnaud Dechesne ◽

Barth F Smets ◽

...

Keyword(s):

Bacterial Community ◽

16S Rrna ◽

16S Rrna Gene ◽

Sequence Similarity ◽

Amplicon Sequencing ◽

Plasmid Transfer ◽

Rrna Gene ◽

Long Term Effects ◽

Cu Contamination ◽

Soil Bacterial

ABSTRACT Stressors like metals or antibiotics can affect bacterial community permissiveness for plasmid uptake, but there is little knowledge about long-term effects of such stressors on the evolution of community permissiveness. We assessed the effect of more than 90 years of soil Cu contamination on bacterial community permissiveness (i.e. uptake ability) toward a gfp-tagged IncP-1 plasmid (pKJK5) introduced via an Escherichia coli donor. Plasmid transfer events from the donor to the recipient soil bacterial community were quantified and transconjugants were subsequently isolated by fluorescence activated cell sorting and identified by 16S rRNA gene amplicon sequencing. Transfer frequency of plasmid pKJK5 was reduced in bacterial communities extracted from highly Cu contaminated (4526 mg kg−1) soil compared to corresponding communities extracted from moderately (458 mg kg−1) Cu contaminated soil and a low Cu reference soil (15 mg kg−1). The taxonomic composition of the transconjugal pools showed remarkable similarities irrespective of the degree of soil Cu contamination and despite contrasting compositions of the extracted recipient communities and the original soil communities. Permissiveness assessed at the level of individual operational taxonomic units (OTUs; 16S rRNA gene 97% sequence similarity threshold) was only slightly affected by soil Cu level and high replicate variability of OTU-level permissiveness indicated a role of stochastic events in IncP-1 plasmid transfer or strain-to-strain permissiveness variability.

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Bacterial diversity of the Inner Mongolian Baer Soda Lake as revealed by 16S rRNA gene sequence analyses

Extremophiles ◽

10.1007/s00792-003-0358-z ◽

2004 ◽

Vol 8 (1) ◽

pp. 45-51 ◽

Cited By ~ 54

Author(s):

Peijin Zhou ◽

Yanhe Ma ◽

Weizhou Zhang ◽

Yanfen Xue ◽

Antonio Ventosa ◽

...

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Bacterial Diversity ◽

Gene Sequence ◽

Soda Lake ◽

16S Rrna Gene Sequence ◽

Rrna Gene ◽

Rrna Gene Sequence ◽

Sequence Analyses

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Bacterial diversity analysis of pork longissimus lumborum following long term ohmic cooking and water bath cooking by amplicon sequencing of 16S rRNA gene

Meat Science ◽

10.1016/j.meatsci.2016.09.007 ◽

2017 ◽

Vol 123 ◽

pp. 97-104 ◽

Cited By ~ 21

Author(s):

Xiaojing Tian ◽

Wei Wu ◽

Qianqian Yu ◽

Man Hou ◽

Fang Gao ◽

...

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Bacterial Diversity ◽

Amplicon Sequencing ◽

Water Bath ◽

Rrna Gene ◽

Diversity Analysis ◽

Longissimus Lumborum

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Comparative Analysis of Bacterial Diversity in Freshwater Sediment of a Shallow Eutrophic Lake by Molecular and Improved Cultivation-Based Techniques

Applied and Environmental Microbiology ◽

10.1128/aem.71.4.2162-2169.2005 ◽

2005 ◽

Vol 71 (4) ◽

pp. 2162-2169 ◽

Cited By ~ 176

Author(s):

Hideyuki Tamaki ◽

Yuji Sekiguchi ◽

Satoshi Hanada ◽

Kazunori Nakamura ◽

Nakao Nomura ◽

...

Keyword(s):

Comparative Analysis ◽

16S Rrna ◽

16S Rrna Gene ◽

Bacterial Diversity ◽

Clone Library ◽

Eutrophic Lake ◽

Freshwater Sediment ◽

Gene Clone ◽

Rrna Gene ◽

Shallow Eutrophic Lake

ABSTRACT Comparative analysis of bacterial diversity in freshwater sediment collected from a shallow eutrophic lake was performed by using 16S rRNA gene clone library and improved cultivation-based techniques. Our study demonstrated that the use of gellan gum as a gelling reagent instead of agar was more effective at increasing culturability, cultivating a diverse array of novel microbes, and reducing the gaps of the results between molecular and cultivation-based analyses.

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