scholarly journals A Tandem Duplicate of Anti-Müllerian Hormone with a Missense SNP on the Y Chromosome Is Essential for Male Sex Determination in Nile Tilapia, Oreochromis niloticus

PLoS Genetics ◽  
2015 ◽  
Vol 11 (11) ◽  
pp. e1005678 ◽  
Author(s):  
Minghui Li ◽  
Yunlv Sun ◽  
Jiue Zhao ◽  
Hongjuan Shi ◽  
Sheng Zeng ◽  
...  
Keyword(s):  
Sex Determination ◽  
Y Chromosome ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Male Sex

scholarly journals 22. PENGARUH UMUR LARVA IKAN NILA (Oreochromis niloticus) TERHADAP PENINGKATAN PENJANTANAN MENGGUNAKAN HORMON METHYL TESTOSTERONE (MT) ALAMI Effect of Tilapia (Oreochromis niloticus) Age in Masculinization Process Using Methyl Testosterone (MT) Natural Hormones

2016 ◽  
Vol 8 (1) ◽  
Author(s):  
Rosmaidar R
Keyword(s):  
Key Words ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Average Percentage ◽  
Natural Hormone ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Male Sex ◽  
Natural Hormones

This research aims to know the influence of larvae of nile tilapia (Oreochromis niloticus) age on masculinization process by the administration of methyl testosterone (MT) natural hormones. This research used 270 nile tilapia larvae divided into 3 age of groups: 5 days (PI), 10 days (PII), and 20 days (PIII), with three repetitions. Larvae were dipped in MT natural hormone for 20 days then reared until age of 60 days, followed by observations of fish sex. The average percentage of male-sex of tilapia fish after dipping with methyl testosterone natural hormone on group PI, PII, and PIII were 68.07%, 66.47%, and 57.29%, respectively. It can be concluded that the age of larvae do not affect the percentage of masculinization process of nile tilapia fish. ____________________________________________________________________________________________________________________ Key words: larvae tilapia, masculinization, methyl testosterone natural hormone

scholarly journals A high quality assembly of the Nile Tilapia (Oreochromis niloticus) genome reveals the structure of two sex determination regions

BMC Genomics ◽  
2017 ◽  
Vol 18 (1) ◽  
Author(s):  
Matthew A. Conte ◽  
William J. Gammerdinger ◽  
Kerry L. Bartie ◽  
David J. Penman ◽  
Thomas D. Kocher
Keyword(s):  
Sex Determination ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
High Quality ◽  
Nile Tilapia Oreochromis Niloticus

scholarly journals Exclusion of two microsatellite markers in the sex-linkage study of Nile tilapia (Oreochromis niloticus L.)

2019 ◽  
Vol 6 (1) ◽  
pp. 143-151
Author(s):  
Marufa Sultana Mitu ◽  
Antima Gani ◽  
Md Bakhtiar Abid ◽  
Sadia Nusrat Sharna ◽  
Farzana Yesmin ◽  
...  
Keyword(s):  
Sex Determination ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sex Linkage ◽  
Female Progeny ◽  
Male Progeny ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Determination System ◽  
Sex Determination System

Monosex Nile tilapia (Oreochromis niloticus) is highly preferred in semi-intensive and intensive culture systems to prevent uncontrolled reproduction and to obtain fast growing male. Production of all male tilapia is being practiced by the hatcheries of Bangladesh mainly by administering androgen hormones (particularly 17-α-methyl-testosterone) with feed in a mixture of undifferentiated fry for about a month. The direct application of hormone to such food chain often arises question in respect to public health and safety. The alternative to this is the production of putative supermales, a rather safe but longer procedure to obtain all male progeny. However, sex determination system in tilapia is fairly complex. Recent developments have resulted in a linkage map and genetic markers that can be used to analyze the sex determination system. For genetic analysis of different genotypes of fish, microsatellite DNA marker ARO120 and ARO121 were used for studying the inheritance pattern for possible sex linkage using Polyacrylamide gel electrophoresis. In case of ARO120, it was observed that the Dam XX was heterozygous; 11 out of 22 female progeny and 10 out of 22 male progeny were found to be heterozygous. In case of ARO121, it was observed that the Dam XX was heterozygous; 16 out of 22 female progeny and 20 out of 22 male progeny were found to be heterozygous. Though the marker polymorphisms were observed in this study, these were excluded from the sexlinkage study due to limited extent of information as sex-linked markers in Nile tilapia BFRI strain. This study provides a baseline for further research using other suitable polymorphic markers for assisting marker-assisted selection. Res. Agric., Livest. Fish.6(1): 143-151, April 2019

scholarly journals Validation of clonal line females for sex determination studies in nile tilapia Oreochromis niloticus L.

2015 ◽  
Vol 1 (1) ◽  
pp. 147-158
Author(s):  
Mohd Golam Quader Khan ◽  
Brendan J McAndrew ◽  
David J Penman
Keyword(s):  
Sex Determination ◽  
Dna Markers ◽  
Oreochromis Niloticus ◽  
Microsatellite Dna ◽  
Nile Tilapia ◽  
Clonal Line ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Microsatellite Dna Markers ◽  
Sequencing Studies ◽  
Sexually Mature

Sex determination in the Nile tilapia Oreochromis niloticus is more complex than a simple XX-XY sex determining mechanism, as evidenced from fairly frequent unexpected sex ratios in progeny. The production of uniform, homozygous experimental material is particularly advantageous for studying sex determining mechanism as well as for the genetic mapping and genome sequencing studies in which interpretations are facilitated by homozygosity. To better understand the genetic mechanism of sex determination, a fully inbred line of clonal females (XX) was verified in controlled environmental conditions using test crosses and microsatellite DNA markers from the tilapia linkage map. A total of successfully amplified 87 microsatellite DNA markers covering all 24 linkage groups were selected for screening sexually mature females from this line. 67 markers were found polymorphic in outbred individuals screened. Markers from LG1, LG3 and LG23 were given more emphasis because sex determining genes have been mapped on these LGs in different species of tilapia. The verification and validation of this clonal line of females made them an important resource to use as a ‘standard reference line’ in genomics, sex determination studies and other studies in Nile tilapia. DOI: http://dx.doi.org/10.3329/ralf.v1i1.22378 Res. Agric., Livest. Fish.1(1): 147-158, Dec 2014

Fine-mapping of a locus on linkage group 23 for sex determination in Nile tilapia (Oreochromis niloticus)

2010 ◽  
Vol 42 (2) ◽  
pp. 222-224 ◽  
Author(s):  
O. Eshel ◽  
A. Shirak ◽  
J. I. Weller ◽  
T. Slossman ◽  
G. Hulata ◽  
...  
Keyword(s):  
Linkage Group ◽  
Sex Determination ◽  
Fine Mapping ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Nile Tilapia Oreochromis Niloticus

The effect of vaccination in controling bacterial disease caused by Aeromonas hydrophila in nile tilapia (Oreochromis niloticus)

2014 ◽  
pp. 27
Author(s):  
Arne A Ratulangi ◽  
Reiny Tumbol ◽  
Hengky Manoppo ◽  
Henneke Pangkey
Keyword(s):  
Survival Rate ◽  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Bacterial Disease ◽  
Immersion Method ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Different Ages ◽  
Motile Aeromonas Septicaemia

This study aims to apply vaccination against bacterial disease. The purpose of vaccination is to trigger the immune respone both non-specific and specific of fish against bacteria Motile Aeromonas Septicaemia (MAS) caused by Aeromonas hydrophila. The vaccination for fish with different ages: 2-3 weeks and 5 weeks were done using immersion method. The fish were re-vaccinated (booster) after two weeks of the first vaccination. The survival rate was < 50 % for juveniles 2-3 weeks and > 50% for juveniles 5 weeks. Survival rate for juveniles 5 weeks was higher than juveniles of 2-3 weeks. This shows that organs of juveniles of 5 weeks were more complete than the 2-3 weeks juveniles. The age of fish is one of the important factors for successfully vaccination. Penelitian ini bertujuan untuk melakukan penerapan vaksinasi terhadap penyakit bakterial. Vaksinasi ditujukan untuk merangsang respon kekebalan non- spesifik dan spesifik pada tubuh ikan terhadap penyakit Motile Aeromonas Septicaemia (MAS) yang disebabkan oleh bakteri Aeromonas hydrophila. Vaksin diberikan pada benih ikan nila yang berbeda umur yaitu 2-3 minggu dan 5 minggu dengan menggunakan metode perendaman. Ikan divaksinasi ulang (booster) setelah 2 (dua) minggu dari vaksinasi yang pertama. Prosentase kelangsungan hidup < 50 % untuk benih umur 2-3 minggu dan > 50% untuk benih umur 5 minggu. Jumlah kematian benih umur 2-3 minggu lebih tinggi dari 5 minggu. Hal ini menunjukkan bahwa fungsi organ benih umur 5 minggu telah lebih lengkap dari pada benih umur 2-3 minggu. Umur ikan merupakan salah satu faktor penting penentu keberhasilan suatu kegiatan vaksinasi.

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