scholarly journals Comprehensive analysis of central carbon metabolism illuminates connections between nutrient availability, growth rate, and cell morphology in Escherichia coli

PLoS Genetics ◽  
2018 ◽  
Vol 14 (2) ◽  
pp. e1007205 ◽  
Author(s):  
Corey S. Westfall ◽  
Petra Anne Levin
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Nutrient Availability ◽  
Cell Morphology ◽  
Carbon Metabolism ◽  
Central Carbon Metabolism ◽  
Comprehensive Analysis ◽  
Central Carbon

scholarly journals Comprehensive analysis of central carbon metabolism reveals multiple connections between nutrients, biosynthetic capacity, and cell morphology inEscherichia coli

2017 ◽  
Author(s):  
Corey S. Westfall ◽  
Petra Anne Levin
Keyword(s):  
Growth Rate ◽  
Nutrient Availability ◽  
Carbon Metabolism ◽  
Complex Trait ◽  
Central Carbon Metabolism ◽  
Bacterial Cells ◽  
Metabolic Genes ◽  
Highly Sensitive ◽  
Central Carbon ◽  
Bacterial Morphology

AbstractBacterial morphology is a complex trait that is highly sensitive to changes in the environment. For heterotrophic organisms, such asEscherichia coli, increases in nutrient levels are frequently accompanied by several-fold increases in both size and growth rate. Despite the dramatic nature of these changes, how alterations in nutrient availability translate into changes in growth and morphology remains a largely open question. To understand the signaling networks coupling nutrient availability with size and shape, we examined the impact of deletions in the entirety of non-essential central carbon metabolic genes onE. coligrowth rate and cell size. Our data reveal the presence of multiple metabolic nodes that play important yet distinctive roles in shaping the cell. Consistent with recent work from our lab and others, although both are sensitive to nutrient availability, size and growth rate vary independently. Cell width and length also appear to be independent phenomena, influenced by different aspects of central carbon metabolism. These findings highlight the diversity of factors that can impact cell morphology and provide a foundation for further studies.Author summaryOften taken for granted, the shape of bacterial cells is a complex trait that is highly sensitive to environmental perturbations. Nutrients in particular, strongly impact bacterial morphology together with growth rate. The ubiquitous, rod-shaped bacteriaEscherichia coliincreases both length and width several fold upon a shift from nutrient poor to nutrient rich medium, a change accompanied by an equally dramatic increase in growth rate. Central carbon metabolism is an obvious site for the integration of nutrient dependent signals that dictate cell size and shape. To develop a clearer picture of the molecular mechanisms coupling nutrient assimilation with cell growth and morphology, we screened the entirety of nonessential carbon metabolic genes for their contribution to growth rate and cell shape. Our data reveal the presence of multiple regulatory circuits coordinating different metabolic pathways with specific aspects of cell growth and morphology. Together, these data firmly establish a role for central carbon metabolism as an environmentally sensitive sculptor of bacterial cells.

Genetic manipulation of Escherichia coli central carbon metabolism for efficient production of fumaric acid

2018 ◽  
Vol 270 ◽  
pp. 96-102 ◽  
Author(s):  
Huan Liu ◽  
Ruirui Song ◽  
Yue Liang ◽  
Ting Zhang ◽  
Li Deng ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fumaric Acid ◽  
Carbon Metabolism ◽  
Genetic Manipulation ◽  
Central Carbon Metabolism ◽  
Efficient Production ◽  
Central Carbon

Improvement of hydrogen production by metabolic engineering of Escherichia coli: Modification on both the PTS system and central carbon metabolism

2020 ◽  
Vol 45 (9) ◽  
pp. 5687-5696 ◽  
Author(s):  
Victor E. Balderas-Hernandez ◽  
Kathya P. Landeros Maldonado ◽  
Arturo Sánchez ◽  
Adam Smoliński ◽  
Antonio De Leon Rodriguez
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
Hydrogen Production ◽  
Carbon Metabolism ◽  
Central Carbon Metabolism ◽  
Central Carbon

Modeling the interaction between the central carbon metabolism of Escherichia coli and bioreactor culture media

2020 ◽  
Vol 163 ◽  
pp. 107753
Author(s):  
Fabián A. Ortega-Quintana ◽  
Mauricio A. Trujillo-Roldán ◽  
Héctor Botero-Castro ◽  
Hernan Alvarez
Keyword(s):  
Escherichia Coli ◽  
Carbon Metabolism ◽  
Culture Media ◽  
Central Carbon Metabolism ◽  
Bioreactor Culture ◽  
Central Carbon

scholarly journals The Role of Metabolites in the Link between DNA Replication and Central Carbon Metabolism in Escherichia coli

Genes ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 447
Author(s):  
Klaudyna Krause ◽  
Monika Maciąg-Dorszyńska ◽  
Anna Wosinski ◽  
Lidia Gaffke ◽  
Joanna Morcinek-Orłowska ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Dna Replication ◽  
Protein Interactions ◽  
Carbon Metabolism ◽  
Molecular Mechanisms ◽  
Central Carbon Metabolism ◽  
Replication Initiation ◽  
Cell Physiology ◽  
E Coli ◽  
Central Carbon

A direct link between DNA replication regulation and central carbon metabolism (CCM) has been previously demonstrated in Bacillus subtilis and Escherichia coli, as effects of certain mutations in genes coding for replication proteins could be specifically suppressed by particular mutations in genes encoding CCM enzymes. However, specific molecular mechanism(s) of this link remained unknown. In this report, we demonstrate that various CCM metabolites can suppress the effects of mutations in different replication genes of E. coli on bacterial growth, cell morphology, and nucleoid localization. This provides evidence that the CCM-replication link is mediated by metabolites rather than direct protein-protein interactions. On the other hand, action of metabolites on DNA replication appears indirect rather than based on direct influence on the replication machinery, as rate of DNA synthesis could not be corrected by metabolites in short-term experiments. This corroborates the recent discovery that in B. subtilis, there are multiple links connecting CCM to DNA replication initiation and elongation. Therefore, one may suggest that although different in detail, the molecular mechanisms of CCM-dependent regulation of DNA replication are similar in E. coli and B. subtilis, making this regulation an important and common constituent of the control of cell physiology in bacteria.

scholarly journals Development of an accurate kinetic model for the central carbon metabolism of Escherichia coli

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Nusrat Jahan ◽  
Kazuhiro Maeda ◽  
Yu Matsuoka ◽  
Yurie Sugimoto ◽  
Hiroyuki Kurata
Keyword(s):  
Escherichia Coli ◽  
Kinetic Model ◽  
Carbon Metabolism ◽  
Central Carbon Metabolism ◽  
Central Carbon
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