scholarly journals Salt responsive alternative splicing of a RING finger E3 ligase modulates the salt stress tolerance by fine-tuning the balance of COP9 signalosome subunit 5A

PLoS Genetics ◽  
2021 ◽  
Vol 17 (11) ◽  
pp. e1009898
Author(s):  
Yuan Zhou ◽  
Xiao-Hu Li ◽  
Qian-Huan Guo ◽  
Peng Liu ◽  
Ying Li ◽  
...  
Keyword(s):  
Salt Stress ◽  
Alternative Splicing ◽  
Plant Development ◽  
Ring Finger ◽  
E3 Ligase ◽  
26S Proteasome ◽  
Fine Tuning ◽  
Cop9 Signalosome ◽  
Salt Stress Response ◽  
Phenotype Analysis

Increasing evidence points to the tight relationship between alternative splicing (AS) and the salt stress response in plants. However, the mechanisms linking these two phenomena remain unclear. In this study, we have found that Salt-Responsive Alternatively Spliced gene 1 (SRAS1), encoding a RING-Type E3 ligase, generates two splicing variants: SRAS1.1 and SRAS1.2, which exhibit opposing responses to salt stress. The salt stress-responsive AS event resulted in greater accumulation of SRAS1.1 and a lower level of SRAS1.2. Comprehensive phenotype analysis showed that overexpression of SRAS1.1 made the plants more tolerant to salt stress, whereas overexpression of SRAS1.2 made them more sensitive. In addition, we successfully identified the COP9 signalosome 5A (CSN5A) as the target of SRAS1. CSN5A is an essential player in the regulation of plant development and stress. The full-length SRAS1.1 promoted degradation of CSN5A by the 26S proteasome. By contrast, SRAS1.2 protected CSN5A by competing with SRAS1.1 on the same binding site. Thus, the salt stress-triggered AS controls the ratio of SRAS1.1/SRAS1.2 and switches on and off the degradation of CSN5A to balance the plant development and salt tolerance. Together, these results provide insights that salt-responsive AS acts as post-transcriptional regulation in mediating the function of E3 ligase.

scholarly journals The RING E3 ligase SDIR1 destabilizes EBF1/EBF2 and modulates the ethylene response to ambient temperature fluctuations in Arabidopsis

2021 ◽  
Vol 118 (6) ◽  
pp. e2024592118
Author(s):  
Dongdong Hao ◽  
Lian Jin ◽  
Xing Wen ◽  
Feifei Yu ◽  
Qi Xie ◽  
...  
Keyword(s):  
Ambient Temperature ◽  
Stress Responses ◽  
Ring Finger ◽  
E3 Ligase ◽  
26S Proteasome ◽  
Fine Tuning ◽  
Temperature Changes ◽  
Ring E3 Ligase ◽  
Ethylene Response ◽  
As Stress

The gaseous phytohormone ethylene mediates numerous aspects of plant growth and development as well as stress responses. The F-box proteins EIN3-binding F-box protein 1 (EBF1) and EBF2 are key components that ubiquitinate and degrade the master transcription factors ethylene insensitive 3 (EIN3) and EIN3-like 1 (EIL1) in the ethylene response pathway. Notably, EBF1 and EBF2 themselves undergo the 26S proteasome-mediated proteolysis induced by ethylene and other stress signals. However, despite their importance, little is known about the mechanisms regulating the degradation of these proteins. Here, we show that a really interesting new gene (RING)-type E3 ligase, salt- and drought-induced ring finger 1 (SDIR1), positively regulates the ethylene response and promotes the accumulation of EIN3. Further analyses indicate that SDIR1 directly interacts with EBF1/EBF2 and targets them for ubiquitination and proteasome-dependent degradation. We show that SDIR1 is required for the fine tuning of the ethylene response to ambient temperature changes by mediating temperature-induced EBF1/EBF2 degradation and EIN3 accumulation. Thus, our work demonstrates that SDIR1 functions as an important modulator of ethylene signaling in response to ambient temperature changes, thereby enabling plant adaptation under fluctuating environmental conditions.

scholarly journals The RING finger E3 ligase STRF1 is involved in membrane trafficking and modulates salt-stress response inArabidopsis thaliana

2015 ◽  
Vol 82 (1) ◽  
pp. 81-92 ◽  
Author(s):  
Miaomiao Tian ◽  
Lijuan Lou ◽  
Lijing Liu ◽  
Feifei Yu ◽  
Qingzhen Zhao ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Membrane Trafficking ◽  
Ring Finger ◽  
E3 Ligase ◽  
Salt Stress Response

scholarly journals The RING Finger E3 Ligase SpRing is a Positive Regulator of Salt Stress Signaling in Salt-Tolerant Wild Tomato Species

2016 ◽  
Vol 57 (3) ◽  
pp. 528-539 ◽  
Author(s):  
Shilian Qi ◽  
Qingfang Lin ◽  
Huishan Zhu ◽  
Fenghua Gao ◽  
Wenhao Zhang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Ring Finger ◽  
E3 Ligase ◽  
Stress Signaling ◽  
Salt Tolerant ◽  
Wild Tomato ◽  
Wild Tomato Species ◽  
Positive Regulator ◽  
Tomato Species

scholarly journals Gene expression patterns in shoots of Camelina sativa with enhanced salinity tolerance provided by plant growth promoting bacteria producing 1-aminocyclopropane-1-carboxylate deaminase or expression of the corresponding acdS gene

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zohreh Heydarian ◽  
Margaret Gruber ◽  
Cathy Coutu ◽  
Bernard R. Glick ◽  
Dwayne D. Hegedus
Keyword(s):  
Salt Stress ◽  
Plant Growth ◽  
Acc Deaminase ◽  
Camelina Sativa ◽  
Fine Tuning ◽  
Plant Growth Promoting Bacteria ◽  
Salt Stress Response ◽  
Expression Of Genes ◽  
Plant Growth Promoting ◽  
Growth Promoting

AbstractGrowth of plants in soil inoculated with plant growth promoting bacteria (PGPB) producing 1-aminocyclopropane-1-carboxylate (ACC) deaminase or expression of the corresponding acdS gene in transgenic lines reduces the decline in shoot length, shoot weight and photosynthetic capacity triggered by salt stress in Camelina sativa. Reducing the levels of ethylene attenuated the salt stress response as inferred from decreases in the expression of genes involved in development, senescence, chlorosis and leaf abscission that are highly induced by salt to levels that may otherwise have a negative effect on plant growth and productivity. Growing plants in soil treated with Pseudomonas migulae 8R6 negatively affected ethylene signaling, auxin and JA biosynthesis and signalling, but had a positive effect on the regulation of genes involved in GA signaling. In plants expressing acdS, the expression of the genes involved in auxin signalling was positively affected, while the expression of genes involved in cytokinin degradation and ethylene biosynthesis were negatively affected. Moreover, fine-tuning of ABA signaling appears to result from the application of ACC deaminase in response to salt treatment. Moderate expression of acdS under the control of the root specific rolD promoter or growing plants in soil treated with P. migulae 8R6 were more effective in reducing the expression of the genes involved in ethylene production and/or signaling than expression of acdS under the more active Cauliflower Mosaic Virus 35S promoter.

Molecular Functions of Rice Cytosol-Localized RING Finger Protein 1 in Response to Salt and Drought and Comparative Analysis of Its Grass Orthologs

2019 ◽  
Vol 60 (11) ◽  
pp. 2394-2409 ◽  
Author(s):  
Yong Chan Park ◽  
Seung Young Choi ◽  
Jong Ho Kim ◽  
Cheol Seong Jang
Keyword(s):  
Higher Plants ◽  
Ring Finger ◽  
E3 Ligase ◽  
26S Proteasome ◽  
Dependent Manner ◽  
Ring Finger Protein ◽  
Target Proteins ◽  
Finger Protein ◽  
Molecular Functions ◽  
Salt And Drought Stresses

Abstract In higher plants, the post-translational modification of target proteins via the attachment of molecules such as ubiquitin (Ub) mediates a variety of cellular functions via the Ub/26S proteasome system. Here, a really interesting new gene (RING)-H2 type E3 ligase, which regulates target proteins via the Ub/26S proteasome system, was isolated from a rice plant, and its other grass orthologs were examined to determine the evolution of its molecular function during speciation. The gene encoding Oryza sativa cytoplasmic-localized RING finger protein 1 (OsCLR1) was highly expressed under salt and drought stresses. By contrast, the three grass orthologs, SbCLR1 from Sorghum bicolor, ZmCLR1 from Zea mays and TaCLR1 from Triticum aestivum, showed different responses to these stresses. Despite these differences, all four orthologs exhibited E3 ligase activity with cytosol-targeted localization, demonstrating conserved molecular functions. Although OsCLR1-overexpressing plants showed higher survival rates under both salt and drought stresses than that of the wild type (WT) plants, this pattern was not observed in the other orthologs. In addition, OsCLR1-overexpressing plants exhibited lower germination rates in ABA than that of WT plants, whereas the three ortholog CLR1-overexpressing plants showed rates similar to the WT plants. These results indicate the positive regulation of OsCLR1 in response to salt and drought in an ABA-dependent manner. Despite the molecular functions of the three CLR1 orthologs remaining largely unknown, our results provide an insight into the evolutionary fate of CLR1 grass orthologs during speciation after the divergence from a common ancestor.

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