Are Melanoides tuberculata and Tarebia granifera (Gastropoda, Thiaridae), suitable first intermediate hosts of Clonorchis sinensis in Vietnam?

Background Two thiarid snail species, Melanoides tuberculata and Tarebia granifera have been reported as first intermediate hosts of the liver fluke Clonorchis sinensis; however, their role as true first intermediate hosts has not been verified. Thus, the present study aimed to clarify the suitability of these two snail species as first intermediate hosts of C. sinensis. This was accomplished by collecting snails from a highly endemic area for C. sinensis in Vietnam, the Thac Ba reservoir, and identifying shed cercariae using molecular techniques. We also conducted experimental infections of five snail species including M. tuberculata and T. granifera with eggs of C. sinensis. Methodology/Principal findings A total of 11,985 snails, representing 10 species were sampled. Five snail species, M. tuberculata, T. granifera, Lymnaea swinhoei, Parafossarulus manchouricus, and Bithynia fuchsiana were found shedding cercariae with an overall prevalence of infection ranging from 0.7% to 11.5%. Seven cercarial types were recorded. Cercariae of C. sinensis were only found in Parafossarulus manchouricus. Using a multiplex PCR approach for detecting C. sinensis infection, the prevalence in P. manchouricus was 4.2%. Additionally, all five snail species were experimentally exposed to C. sinensis eggs, however only P. manchouricus was successfully infected with an infection rate of 7.87%. Conclusions/Significance We confirmed that in the Thac Ba reservoir, Vietnam, the two thiarids, M. tuberculata and T. granifera are not suitable first intermediate hosts of C. sinensis. Only P. manchouricus was found infected by C. sinensis in nature, and was the only species that became infected experimentally.

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Biological control of the invasive snail species Melanoides tuberculata and Tarebia granifera in Zaporizka Nuclear Power Plant cooling pond

Ukrainian Journal of Ecology ◽

10.15421/2018_301 ◽

2018 ◽

Vol 8 (1) ◽

pp. 975-982

Author(s):

V. Yakovenko ◽

O. Fedonenko ◽

O. Klimenko ◽

O. Petrovsky

Keyword(s):

Biological Control ◽

Power Plant ◽

Nuclear Power Plant ◽

Nuclear Power ◽

Snail Species ◽

Cooling Pond ◽

Melanoides Tuberculata ◽

Power Plant Cooling ◽

Tarebia Granifera

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Molecular investigation of Neorickettsia risticii in trematodes and snails in a region with serological evidence of this agent in horses, state of Rio de Janeiro

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/1678-4162-8804 ◽

2016 ◽

Vol 68 (6) ◽

pp. 1470-1478

Author(s):

R.L. Costa ◽

G.L.V. Vitari ◽

C.B. Silva ◽

M.P. Peckle ◽

M.S. Pires ◽

...

Keyword(s):

Rio De Janeiro ◽

Molecular Techniques ◽

Snail Species ◽

Rrna Gene ◽

Larval Stages ◽

Melanoides Tuberculata ◽

Target Dna ◽

History Of ◽

Breeding Farms ◽

Snail Tissue

ABSTRACT In Brazil, some studies have indicated that Neorickettsia risticii circulates in horses, but it is unclear which are the possible intermediate vectors of this bacterium in the country. The aim of this study was to use molecular techniques in order to analyze the presence of N. risticii in snails and larval stages of trematodes in farms in a region with a history of seroreactive horses towards this bacterium, in Rio de Janeiro, Brazil. Convenience sampling was used in the studied region. The collected snails were exposed to incandescent light (60W) for 2-4 hours in order to investigate trematodes in larval forms. Deoxyribonucleic acid (DNA) was extracted from snail tissue and trematode. Real-time PCR (qPCR) technique was used to investigate the presence of a 16S rRNA gene fragment of N. risticii. Snail specimens (n=410) were collected from 11 horse-breeding farms, and the following species were identified: Melanoides tuberculata, Pomacea sp., Biomphalaria tenagophila, Physa acuta, Drepanotrema anatinum and Biomphalaria straminea. Only 3.17% (n=13/410) of the collected snails were infected by trematodes. The cercariae obtained from these snails were classified as Megalourous cercariae, Pleurolophocercus cercariae and Furcocercous cercariae. There was no amplification of the target DNA of N. risticii in the snail and trematode samples tested by qPCR. Based on these data, the transmission of N. risticii by trematodes using these snail species in this region does not appear to occur or occurs at very low rates. Thus, further studies are needed in order to clarify which species of invertebrate hosts are infected by this bacterium and potentially participate in the transmission chain of equine neorickettsiosis in the state of Rio de Janeiro, Brazil.

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Are Freshwater Snails, Melanoides sp. and Invasive Tarebia granifera (Gastropoda: Thiaridae) Suitable Intermediate Hosts for Calicophoron microbothrium (Trematoda: Paramphistomoidea)? An Experimental Study

Frontiers in Veterinary Science ◽

10.3389/fvets.2021.705954 ◽

2021 ◽

Vol 8 ◽

Author(s):

Mokgadi P. Malatji ◽

Nkululeko Myende ◽

Samson Mukaratirwa

Keyword(s):

South Africa ◽

Soft Tissues ◽

Morphological Characters ◽

Wide Distribution ◽

Snail Species ◽

Intermediate Hosts ◽

Experimental Conditions ◽

Mpumalanga Province ◽

Filial Generation ◽

Tarebia Granifera

Prosobranch snails and adult Paramphistomoidea flukes were collected from water bodies and cattle abattoir located in Mpumalanga province of South Africa, respectively. The snails were identified based on morphological characters as well as the ITS-2 and 16S markers as Melanoides sp. and Tarebia granifera, respectively, and the Paramphistomoidea flukes were identified as Calicophoron microbothrium using the ITS-1/5.8S/ITS-2 marker. After confirming identification, the snails were bred to first filial generation (F1) under laboratory conditions. Ninety snails were randomly selected from the laboratory-bred F1 snails and 25 Melanoides sp. and 20 T. granifera were exposed to C. microbothrium miracidia, and the same numbers were maintained as non-exposed controls. Results showed that C. microbothrium successfully established in Melanoides sp. and produced cercariae, and the prepatent period recorded was 21 days. Three snails shed cercariae at day 21 postexposure (PE), and rediae and free cercariae were detected in the soft tissues of one snail on dissection at day 44 PE. The same fluke did not establish in T. granifera. Melanoides sp. started producing offspring at day 7 PE, and T. granifera at day 14 PE. In conclusion, our results showed that Melanoides sp. used in this study is a suitable intermediate host for C. microbothrium under experimental conditions, and given the wide distribution of this snail species, it is important to determine its role in the natural transmission of other Calicophoron species that have been reported in South Africa.

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FRESHWATER SNAIL DIVERSITY IN GROWOUT PONDS OF GIANT GOURAMI REGARDING INTERMEDIATE HOSTS OF TREMATODE LARVAE (CERCARIAE STAGE) IN TIEN GIANG PROVINCE

Tạp chí Khoa học ◽

10.54607/hcmue.js.18.9.3262(2021) ◽

2021 ◽

Vol 18 (9) ◽

pp. 1649

Author(s):

Phạm Cử Thiện ◽

Trần Thị Thu Thủy

Keyword(s):

Freshwater Snail ◽

Intermediate Hosts ◽

Melanoides Tuberculata ◽

Tarebia Granifera

Nghiên cứu thành phần loài ốc nước ngọt và tỉ lệ nhiễm sán lá song chủ (giai đoạn cercariae) trên ốc trong ao nuôi cá thịt tai tượng ở huyện Châu Thành, tỉnh Tiền Giang được thực hiện vào tháng 10/2020 và tháng 4/2021. Tổng số 4548 mẫu từ 8 loài ốc, 8 giống, 4 họ đã được thu và phân loại dựa theo đặc điểm hình thái. Kết quả cho thấy, ốc Melanoides tuberculata bị nhiễm Xiphidio cercariae và Furcocercous cercariae trong khi ốc Bithynia sp. nhiễm Xiphidio cercariae và Parapleurolophocercous cercariae. Sáu loài ốc còn lại không nhiễm cercariae bao gồm Filopaludina sumatrensis, Pomacea sp., Sermyla tornatella, Sinotaia lithophaga, Tarebia granifera và Thiara scabra. Tỉ lệ ao nhiễm cercariae trong mùa khô cao hơn trong mùa mưa (P>0.05). Cần tiếp tục nghiên cứu dịch tễ học của sán lá song chủ trong ao nuôi cá tai tượng nhằm góp phần phát triển nuôi trồng thủy sản bền vững và an toàn vệ sinh thực phẩm.

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Global status of fish-borne zoonotic trematodiasis in humans

Acta Parasitologica ◽

10.2478/s11686-013-0155-5 ◽

2013 ◽

Vol 58 (3) ◽

Cited By ~ 56

Author(s):

Nguyen Hung ◽

Henry Madsen ◽

Bernard Fried

Keyword(s):

Liver Fluke ◽

Clonorchis Sinensis ◽

Clinical Aspects ◽

Intermediate Hosts ◽

Highly Pathogenic ◽

Present Information ◽

The Family ◽

Family Level ◽

Global Status ◽

Reservoir Hosts

AbstractFishborne zoonotic trematodes (FZT), infecting humans and mammals worldwide, are reviewed and options for control discussed. Fifty nine species belonging to 4 families, i.e. Opisthorchiidae (12 species), Echinostomatidae (10 species), Heterophyidae (36 species) and Nanophyetidae (1 species) are listed. Some trematodes, which are highly pathogenic for humans such as Clonorchis sinensis, Opisthorchis viverrini, O. felineus are discussed in detail, i.e. infection status in humans in endemic areas, clinical aspects, symptoms and pathology of disease caused by these flukes. Other liver fluke species of the Opisthorchiidae are briefly mentioned with information about their infection rate and geographical distribution. Intestinal flukes are reviewed at the family level. We also present information on the first and second intermediate hosts as well as on reservoir hosts and on habits of human eating raw or undercooked fish.

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A study on the fine structure of Clonorchis sinensis, a liver fluke III.The prostate gland

The Korean Journal of Parasitology ◽

10.3347/kjp.1980.18.1.93 ◽

1980 ◽

Vol 18 (1) ◽

pp. 93 ◽

Cited By ~ 3

Author(s):

Kye Heon Jeong ◽

Han Jong Rim ◽

Chang Whan Kim

Keyword(s):

Fine Structure ◽

Liver Fluke ◽

Clonorchis Sinensis ◽

Prostate Gland

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A morphological study on spermatogenesis in the liver fluke, Clonorchis sinensis

The Korean Journal of Parasitology ◽

10.3347/kjp.1976.14.2.123 ◽

1976 ◽

Vol 14 (2) ◽

pp. 123 ◽

Cited By ~ 5

Author(s):

Kye Heon Jeong ◽

Han Jong Rim ◽

He Young Yang ◽

Woo Kap Kim ◽

Chang Whan Kim

Keyword(s):

Morphological Study ◽

Liver Fluke ◽

Clonorchis Sinensis

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Molecular detection of cattle Sarcocystis spp. in North-West Italy highlights their association with bovine eosinophilic myositis

Parasites & Vectors ◽

10.1186/s13071-021-04722-5 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Selene Rubiola ◽

Tiziana Civera ◽

Felice Panebianco ◽

Davide Vercellino ◽

Francesco Chiesa

Keyword(s):

18S Rdna ◽

Inflammatory Myopathy ◽

Molecular Techniques ◽

Diaphragm Muscle ◽

Economic Losses ◽

Intermediate Hosts ◽

Slaughter Cattle ◽

Significant Difference ◽

Pcr Targeting

Abstract Background Cattle are intermediate hosts of six Sarcocystis species, among which Sarcocystis hominis and Sarcocystis heydorni can infect humans through the consumption of raw or undercooked meat. In addition to the zoonotic potential, there is increasing interest in these protozoa because of the evidence supporting the role of Sarcocystis spp. in the occurrence of bovine eosinophilic myositis (BEM), a specific inflammatory myopathy which leads to carcass condemnation and considerable economic losses. Actually, all the prevalence studies carried out on cattle in Italy have been based on either morphological or 18S rDNA-based molecular techniques, most likely leading to misidentification of closely related species. Therefore, there is a strong need for new data on the prevalence of the different Sarcocystis spp. in cattle in Italy and their association with bovine eosinophilic myositis. Methods To reach our aim, individual striated muscle samples from BEM condemned carcasses (N = 54) and diaphragm muscle samples from randomly sampled carcasses (N = 59) were obtained from Northwest Italy slaughterhouses. Genomic DNA was extracted and analyzed by multiplex-PCR targeting 18S rDNA and cox1 genes. PCR products amplified using the genus-specific primer set in absence of the specific fragment for S. hirsuta, S. cruzi, S. hominis or S. bovifelis were sequenced to achieve species identification. Results Sarcocystis DNA was detected in 67.8% of the samples from slaughter cattle and in 90.7% of the samples from BEM condemned carcasses. S. cruzi was identified as the most prevalent species in slaughter cattle (61%), followed by S. bovifelis (10.2%), S. hominis (8.5%) and S. hirsuta (1.7%). Notably, among the different Sarcocystis spp. detected, the presence of S. bovifelis and S. hominis was significantly higher in samples isolated from BEM condemned carcasses (46.3% and 40.7% respectively), while there was no statistically significant difference between the presence of S. cruzi or S. hirsuta in BEM condemned carcasses (42.6% and 1.8%, respectively) and randomly sampled carcasses. Furthermore, DNA sequence analysis revealed the presence of a putative new species in two carcasses. Conclusions Our study contributes to updating the data on the prevalence of the different Sarcocystis spp. in cattle in Italy, highlighting the presence of three Sarcocystis spp., S. cruzi, S. hominis and S. bovifelis, in BEM lesions and allowing us to speculate on the possible role of S. hominis and S. bovifelis as the major sarcosporidian species involved in bovine eosinophilic myositis. Graphic Abstract

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Prevalence of Linguatula sp., a food-borne zoonotic aberrant arthropod, in river buffaloes slaughtered at Tabriz slaughterhouse, Iran

Transactions of the Royal Society of Tropical Medicine and Hygiene ◽

10.1093/trstmh/traa122 ◽

2020 ◽

Author(s):

Nasser Hajipour ◽

Jennifer Ketzis ◽

Parviz Hassanzadeh

Keyword(s):

Infection Rate ◽

Statistical Association ◽

Intermediate Hosts ◽

Mesenteric Lymph Nodes ◽

Intensity Of Infection ◽

Mesenteric Lymph ◽

Food Borne ◽

Different Seasons ◽

Significant Statistical Association ◽

Age And Sex

Abstract Background Canids and herbivores are the definitive and intermediate hosts of Linguatula sp., respectively. Methods Mesenteric lymph nodes (n=32 525) were randomly collected from 7585 buffaloes from July 2016 to July 2019 and examined macroscopically. Results Results showed that 388 (5.11%) buffaloes were infected. The intensity of infection was determined to be 3.07±0.07. Significant statistical association was identified between infection rate and age and sex. Although there were no significant differences in the infection rate over different seasons, the highest infection rate was observed in autumn. Conclusions These data highlight the importance of inspection at slaughter.

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