scholarly journals Putative Zinc Finger Protein Binding Sites Are Over-Represented in the Boundaries of Methylation-Resistant CpG Islands in the Human Genome

PLoS ONE ◽  
2007 ◽  
Vol 2 (11) ◽  
pp. e1184 ◽  
Author(s):  
Shicai Fan ◽  
Fang Fang ◽  
Xuegong Zhang ◽  
Michael Q. Zhang
Keyword(s):  
Protein Binding ◽  
Human Genome ◽  
Zinc Finger ◽  
Binding Sites ◽  
Zinc Finger Protein ◽  
Cpg Islands ◽  
Protein Binding Sites ◽  
Finger Protein ◽  
Putative Zinc Finger

scholarly journals The Cardiac Tissue-Restricted Homeobox Protein Csx/Nkx2.5 Physically Associates with the Zinc Finger Protein GATA4 and Cooperatively Activates Atrial Natriuretic Factor Gene Expression

1998 ◽  
Vol 18 (6) ◽  
pp. 3120-3129 ◽  
Author(s):  
Youngsook Lee ◽  
Tetsuo Shioi ◽  
Hideko Kasahara ◽  
Shawn M. Jobe ◽  
Russell J. Wiese ◽  
...  
Keyword(s):  
Gene Expression ◽  
Zinc Finger ◽  
Protein Interaction ◽  
Binding Sites ◽  
Atrial Natriuretic Factor ◽  
Target Gene ◽  
Cardiac Tissue ◽  
Zinc Finger Protein ◽  
Finger Protein ◽  
Homeobox Protein

ABSTRACT Specification and differentiation of the cardiac muscle lineage appear to require a combinatorial network of many factors. The cardiac muscle-restricted homeobox protein Csx/Nkx2.5 (Csx) is expressed in the precardiac mesoderm as well as the embryonic and adult heart. Targeted disruption of Csx causes embryonic lethality due to abnormal heart morphogenesis. The zinc finger transcription factor GATA4 is also expressed in the heart and has been shown to be essential for heart tube formation. GATA4 is known to activate many cardiac tissue-restricted genes. In this study, we tested whether Csx and GATA4 physically associate and cooperatively activate transcription of a target gene. Coimmunoprecipitation experiments demonstrate that Csx and GATA4 associate intracellularly. Interestingly, in vitro protein-protein interaction studies indicate that helix III of the homeodomain of Csx is required to interact with GATA4 and that the carboxy-terminal zinc finger of GATA4 is necessary to associate with Csx. Both regions are known to directly contact the cognate DNA sequences. The promoter-enhancer region of the atrial natriuretic factor (ANF) contains several putative Csx binding sites and consensus GATA4 binding sites. Transient-transfection assays indicate that Csx can activate ANF reporter gene expression to the same extent that GATA4 does in a DNA binding site-dependent manner. Coexpression of Csx and GATA4 synergistically activates ANF reporter gene expression. Mutational analyses suggest that this synergy requires both factors to fully retain their transcriptional activities, including the cofactor binding activity. These results demonstrate the first example of homeoprotein and zinc finger protein interaction in vertebrates to cooperatively regulate target gene expression. Such synergistic interaction among tissue-restricted transcription factors may be an important mechanism to reinforce tissue-specific developmental pathways.

scholarly journals The putative zinc-finger protein WZF1 interacts with a cis-acting element of wheat histone genes

1993 ◽  
Vol 217 (3) ◽  
pp. 1049-1056 ◽  
Author(s):  
Ayako SAKAMOTO ◽  
Maki MINAMI ◽  
Gyung Hye HUH ◽  
Masaki IWABUCHI
Keyword(s):  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Histone Genes ◽  
Cis Acting ◽  
Finger Protein ◽  
Putative Zinc Finger

scholarly journals Repression of hsp70 heat shock gene transcription by the suppressor of hairy-wing protein of Drosophila melanogaster.

1991 ◽  
Vol 11 (4) ◽  
pp. 1894-1900 ◽  
Author(s):  
C Holdridge ◽  
D Dorsett
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Protein Binding ◽  
Protein Interactions ◽  
Binding Sites ◽  
Zinc Finger Protein ◽  
Gene Promoter ◽  
Heat Shock Gene ◽  
Protein Binding Sites ◽  
Shock Gene

The suppressor of hairy-wing [su(Hw)] locus of Drosophila melanogaster encodes a zinc finger protein that binds a repeated motif in the gypsy retroposon. Mutations of su(Hw) suppress the phenotypes associated with mutations caused by gypsy insertions. To examine the mechanisms by which su(Hw) alters gene expression, a fragment of gypsy containing multiple su(Hw) protein-binding sites was inserted into various locations in the well-characterized Drosophila hsp70 heat shock gene promoter. We found no evidence for activation of basal hsp70 transcription by su(Hw) protein in cultured Drosophila cells but observed that it can repress heat shock-induced transcription. Repression occurred only when su(Hw) protein-binding sites were positioned between binding sites for proteins required for heat shock transcription. We propose that su(Hw) protein interferes nonspecifically with protein-protein interactions required for heat shock transcription, perhaps sterically, or by altering the ability of DNA to bend or twist.

Identification of a KRAB-Zinc Finger Protein Binding to theRpe65Gene Promoter

2006 ◽  
Vol 31 (5) ◽  
pp. 457-466 ◽  
Author(s):  
Zhongjian Lu ◽  
Eugenia Poliakov ◽  
T. Michael Redmond
Keyword(s):  
Protein Binding ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Finger Protein

scholarly journals Identification and Analysis of Papillomavirus E2 Protein Binding Sites in the Human Genome

2011 ◽  
Vol 86 (1) ◽  
pp. 348-357 ◽  
Author(s):  
L. Vosa ◽  
A. Sudakov ◽  
M. Remm ◽  
M. Ustav ◽  
R. Kurg
Keyword(s):  
Protein Binding ◽  
Human Genome ◽  
Binding Sites ◽  
Protein Binding Sites ◽  
E2 Protein

Gene Encoding a Putative Zinc Finger Protein inSynechocystisPCC6803

1991 ◽  
Vol 55 (9) ◽  
pp. 2259-2264
Author(s):  
Yutaka Ogura ◽  
Tadashi Yoshida ◽  
Yasukazu Nakamura ◽  
Miho Takemura ◽  
Kenji Oda ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Gene Encoding ◽  
Finger Protein ◽  
Putative Zinc Finger
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