scholarly journals Whole Genome Gene Expression Analysis Reveals Casiopeína-Induced Apoptosis Pathways

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e54664 ◽  
Author(s):  
Alejandra Idan Valencia-Cruz ◽  
Laura I. Uribe-Figueroa ◽  
Rodrigo Galindo-Murillo ◽  
Karol Baca-López ◽  
Anllely G. Gutiérrez ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Whole Genome ◽  
Apoptosis Pathways ◽  
Induced Apoptosis

Global Epigenomic Profiling Demonstrates That Myelodysplasia Is Characterized by a Distinct Epigenetic Signature with Aberrant DNA Methylation Changes Involving Various Malignant and Hematopoietic Pathways.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 2436-2436
Author(s):  
L. Zhou ◽  
J. Opalinska ◽  
D. Sohal ◽  
R. Thompson ◽  
Y. Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Expression Analysis ◽  
Peripheral Blood ◽  
Gene Expression Analysis ◽  
Whole Genome ◽  
Gene Promoters ◽  
Global Methylation ◽  
Peripheral Blood Leucocyte ◽  
Peripheral Blood Leucocytes

Abstract Myelodysplasia (MDS) is a clonal hematopoietic disorder that leads to ineffective hematopoiesis and peripheral cytopenias. DNMT inhibitors such as azacytidine have led to clinical responses in patients, though the genes affected by epigenetic alterations are not well known. Whole genome DNA methylation was analyzed by a recently described novel method, The HELP assay (HpaII tiny fragment Enrichment by Ligation-mediated PCR; Khulan et al, Genome Res. 2006 Aug;16(8)) that uses differential methylation specific restriction digestion by HpaII and MspI followed by amplification, two color labeling and cohybridization to quantitatively determine individual promoter island methylation. A whole genome human promoter array (Nimblegen) was used to determine the level of methylation of 25626 gene promoters by calculating HpaII/MspI cut fragment intensity ratio. Peripheral blood leucocytes from 13 patients with MDS were compared to 9 age matched normal and anemic controls. Gene expression analysis was performed using 37K oligo maskless arrays on cDNA obtained from the same samples. Analysis showed that whole genome methylation profiling has greater discriminatory power in separating clusters of MDS samples from normal and anemic controls when compared to gene expression analysis. Unsupervised clustering based on epigenetic profiling demonstrated that only two cases of early MDS clustered with normals as compared to absolutely no separation between MDS and normals with clustering based on gene expression patterns. A high correlation (r=0.88–0.96) was observed between global methylation profiles of matched sets of bone marrow and peripheral blood leucocyte samples from selected patients demonstrating that peripheral blood leucocytes can be a valid surrogate for epigenomic analysis. Further analysis showed that genes consistently aberrantly methylated in MDS included Syk kinase, HOXB3, several histone acetyltranferases and others. Functional analysis by Ingenuity showed that cancer and cell signaling pathways were the most affected by epigenetic silencing. Most interestingly, a large proportion of gene promoters were also aberrantly hypomethylated. These included genes from Ras oncogene family, the CDC42 GTPase, various methyl binding proteins and other proteins mainly encoding for cancer and hematopoiesis functional pathways, thus biologically validating our analysis. Therefore, our data demonstrates that MDS is characterized by distinct epigenetic aberrations that are preserved in peripheral blood leucocytes. These can be the basis of future studies on pathogenesis and diagnosis for this disease and can potentially uncover a new set of therapeutic gene targets.

GENE EXPRESSION ANALYSIS OF E.COLI INDUCED APOPTOSIS OF HUMAN LYMPHOCYTES AND THE PROTECTIVE EFFECT OF TAT-BH4.

2005 ◽  
Vol 33 ◽  
pp. A135
Author(s):  
Tracey H Wagner ◽  
Kevin W McConnell ◽  
Katherine C Chang ◽  
Jeffrey C Dunne ◽  
Kevin Z Yuan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protective Effect ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Human Lymphocytes ◽  
Induced Apoptosis

scholarly journals Gene Expression Analysis to Evaluate the Effect of p38 Specific Inhibitor SB203580 on SEB‐Induced Apoptosis Related Pathways

2013 ◽  
Vol 27 (S1) ◽  
Author(s):  
Casey Sondgeroth ◽  
Lisa Hesse ◽  
Alyssa Allbaugh ◽  
Chanaka Mendis ◽  
Marti Jett
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Specific Inhibitor ◽  
Induced Apoptosis

scholarly journals A generic approach for the design of whole-genome oligoarrays, validated for genomotyping, deletion mapping and gene expression analysis on Staphylococcus aureus

BMC Genomics ◽  
2005 ◽  
Vol 6 (1) ◽  
Author(s):  
Yvan Charbonnier ◽  
Brian Gettler ◽  
Patrice François ◽  
Manuela Bento ◽  
Adriana Renzoni ◽  
...  
Keyword(s):  
Gene Expression ◽  
Staphylococcus Aureus ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Deletion Mapping ◽  
Whole Genome

scholarly journals Comparison of HepG2 and HepaRG by Whole-Genome Gene Expression Analysis for the Purpose of Chemical Hazard Identification

2010 ◽  
Vol 115 (1) ◽  
pp. 66-79 ◽  
Author(s):  
Danyel G. J. Jennen ◽  
Christina Magkoufopoulou ◽  
Hans B. Ketelslegers ◽  
Marcel H. M. van Herwijnen ◽  
Jos C. S. Kleinjans ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Hazard Identification ◽  
Whole Genome ◽  
Chemical Hazard

Whole-genome gene expression analysis in urine samples of patients with prostate cancer and benign prostate hyperplasia

2017 ◽  
Vol 35 (10) ◽  
pp. 607.e15-607.e24 ◽  
Author(s):  
Taha Reşid Özdemir ◽  
Adnan Şimşir ◽  
Hüseyin Onay ◽  
İbrahim Cüreklibatır ◽  
Ferda Özkınay ◽  
...  
Keyword(s):  
Gene Expression ◽  
Prostate Cancer ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Benign Prostate Hyperplasia ◽  
Urine Samples ◽  
Whole Genome ◽  
Prostate Hyperplasia ◽  
Benign Prostate

scholarly journals Gene expression analysis of glucocorticoid-induced apoptosis by a gene microarray technique

10.1038/87108 ◽  
2001 ◽  
Vol 27 (S4) ◽  
pp. 58-58 ◽  
Author(s):  
Huiling He ◽  
Michael Malone ◽  
Steven Ostrowski ◽  
Clark Distelhorst
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Gene Microarray ◽  
Induced Apoptosis ◽  
Microarray Technique

Gene expression analysis from phoxim-induced domesticated silkworm (Bombyx mori) by whole-genome oligonucleotide microarray

2011 ◽  
Vol 101 (1) ◽  
pp. 48-52 ◽  
Author(s):  
Y.H. Wang ◽  
J.M. Wang ◽  
G.D. Peng ◽  
B.X. Sun ◽  
B. Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bombyx Mori ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Oligonucleotide Microarray ◽  
Whole Genome ◽  
Silkworm Bombyx Mori

Gene expression analysis in chemical-treated dendritic cells: Discriminating contact sensitisers and irritants employing the immune toxicity chip and a whole genome array

2008 ◽  
Vol 180 ◽  
pp. S118
Author(s):  
Sandra Szameit ◽  
Klemens Vierlinger ◽  
Letizia Farmer ◽  
Helga Tuschl ◽  
Christa Nöhammer
Keyword(s):  
Gene Expression ◽  
Dendritic Cells ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Whole Genome ◽  
Genome Array ◽  
Immune Toxicity
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