Nitric Oxide Mediates Root K+/Na+ Balance in a Mangrove Plant, Kandelia obovata, by Enhancing the Expression of AKT1-Type K+ Channel and Na+/H+ Antiporter under High Salinity

As a dominant mangrove species, Kandelia obovata is distributed in an intertidal marsh with an active H2S release. Whether H2S participates in the salt tolerance of mangrove plants is still ambiguous, although increasing evidence has demonstrated that H2S functions in plant responses to multiple abiotic stresses. In this study, NaHS was used as an H2S donor to investigate the regulatory mechanism of H2S on the salt tolerance of K. obovata seedlings by using a combined physiological and proteomic analysis. The results showed that the reduction in photosynthesis (Pn) caused by 400 mM of NaCl was recovered by the addition of NaHS (200 μM). Furthermore, the application of H2S enhanced the quantum efficiency of photosystem II (PSII) and the membrane lipid stability, implying that H2S is beneficial to the survival of K. obovata seedlings under high salinity. We further identified 37 differentially expressed proteins by proteomic approaches under salinity and NaHS treatments. Among them, the proteins that are related to photosynthesis, primary metabolism, stress response and hormone biosynthesis were primarily enriched. The physiological and proteomic results highlighted that exogenous H2S up-regulated photosynthesis and energy metabolism to help K. obovata to cope with high salinity. Specifically, H2S increased photosynthetic electron transfer, chlorophyll biosynthesis and carbon fixation in K. obovata leaves under salt stress. Furthermore, the abundances of other proteins related to the metabolic pathway, such as antioxidation (ascorbic acid peroxidase (APX), copper/zinc superoxide dismutase (CSD2), and pancreatic and duodenal homeobox 1 (PDX1)), protein synthesis (heat-shock protein (HSP), chaperonin family protein (Cpn) 20), nitrogen metabolism (glutamine synthetase 1 and 2 (GS2), GS1:1), glycolysis (phosphoglycerate kinase (PGK) and triosephosphate isomerase (TPI)), and the ascorbate–glutathione (AsA–GSH) cycle were increased by H2S under high salinity. These findings provide new insights into the roles of H2S in the adaptations of the K. obovata mangrove plant to high salinity environments.

Download Full-text

M-Type K+ Channel as Plasma Membrane Nitric Oxide and Reactive Oxygen Species Sensor

Biophysical Journal ◽

10.1016/j.bpj.2012.11.1507 ◽

2013 ◽

Vol 104 (2) ◽

pp. 268a-269a

Author(s):

Lezanne Ooi ◽

Louisa Pettinger ◽

Nikita Gamper

Keyword(s):

Nitric Oxide ◽

Reactive Oxygen Species ◽

Plasma Membrane ◽

Reactive Oxygen ◽

K Channel ◽

Oxygen Species ◽

Type K

Download Full-text

Nitric oxide enhances salt secretion and Na+ sequestration in a mangrove plant, Avicennia marina, through increasing the expression of H+-ATPase and Na+/H+ antiporter under high salinity

Tree Physiology ◽

10.1093/treephys/tpq086 ◽

2010 ◽

Vol 30 (12) ◽

pp. 1570-1585 ◽

Cited By ~ 76

Author(s):

J. Chen ◽

Q. Xiao ◽

F. Wu ◽

X. Dong ◽

J. He ◽

...

Keyword(s):

Nitric Oxide ◽

High Salinity ◽

Avicennia Marina ◽

Mangrove Plant ◽

Salt Secretion

Download Full-text

Transcriptomic Analyses Reveal the Effect of Nitric Oxide on the Lateral Root Development and Growth of Mangrove Plant Kandelia Obovata

10.21203/rs.3.rs-894970/v1 ◽

2021 ◽

Author(s):

Ming-Yue Wei ◽

Huan Li ◽

You-Hui Zhong ◽

Zhi-Jun Shen ◽

Dong-Na Ma ◽

...

Keyword(s):

Nitric Oxide ◽

Cell Wall ◽

Root System ◽

Root Development ◽

Lateral Root ◽

Dependent Manner ◽

Kandelia Obovata ◽

Mangrove Species ◽

Mangrove Plant ◽

Lateral Root Development

Abstract Background and aims Kandelia obovata, a dominant mangrove species in China, produces complex buttress roots and prop roots in intertidal wetlands where high quantities of nitric oxide (NO) are produced by reducing sediments. NO, a key signaling molecule, participates in an array of plant physiological and developmental processes. However, it is unclear whether NO functions in K. obovata root system establishment. Methods Here, we used a transcriptomic approach to investigate the potential role of NO in the regulation of K. obovata lateral root development and growth. Transcript profiles and bioinformatics analyses were used to characterize potential regulatory mechanisms. Results NO enhanced K. obovata lateral root development and growth in a dose-dependent manner. RNA-seq analysis identiﬁed 1,593 differentially expressed genes (DEGs), of which 646 and 947 were up- and down-regulated in roots treated with NO donor. Functional annotation analysis demonstrated that the starch and sucrose pathway was significantly induced in response to NO. A suite of DEGs involved in hormone signal transduction and cell wall metabolism was also differentially regulated by NO. Taken together, our results suggest that a complex interaction between energy metabolism, multiple hormone signaling pathways, and cell wall biosynthesis is required for the NO regulation on lateral root development and growth in mangrove plant K. obovata. Conclusion NO appears to contribute to the formation of the unique root system of mangrove plants.

Download Full-text