scholarly journals Transcriptome Analysis of Chlorantraniliprole Resistance Development in the Diamondback Moth Plutella xylostella

PLoS ONE ◽  
2013 ◽  
Vol 8 (8) ◽  
pp. e72314 ◽  
Author(s):  
Qingsheng Lin ◽  
Fengliang Jin ◽  
Zhendi Hu ◽  
Huanyu Chen ◽  
Fei Yin ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Plutella Xylostella ◽  
Diamondback Moth ◽  
Resistance Development

scholarly journals Increased Responses of Phenoloxidase in Chlorantraniliprole Resistance of Plutella xylostella (Lepidoptera: Plutellidae)

2020 ◽  
Vol 20 (4) ◽  
Author(s):  
Nian-Meng Wang ◽  
Jing-Jing Li ◽  
Ze-Yu Shang ◽  
Qi-Tong Yu ◽  
Chao-Bin Xue
Keyword(s):  
Plutella Xylostella ◽  
Synergistic Effects ◽  
Diamondback Moth ◽  
Cruciferous Vegetables ◽  
Transcriptional Expression ◽  
Resistance Development ◽  
Expression Levels ◽  
Important Pest ◽  
Resistant Strains

Abstract The diamondback moth (Plutella xylostella, DBM) is an important pest of cruciferous vegetables. The use of chlorantraniliprole has been essential in the management of the DBM. However, in many countries and areas, DBM has become highly resistant to chlorantraniliprole. Three different DBM strains, susceptible (S), chlorantraniliprole-selected (Rc), and field-collected (Rb) resistant strains/populations were studied for the role of phenoloxidase in resistance development to the insecticide. By assaying the activity of phenoloxidase (PO) in the three different DBM strains, the results showed that the PO activity in the Rc strain was increased significantly compared with the S strain. The synergistic effects of quercetin showed that the resistant ratio (RR) of the QRc larvae to chlorantraniliprole was decreased from 423.95 to 316.42-fold compared with the Rc larvae. Further studies demonstrated that the transcriptional and translational expression levels of PxPPO1 (P. xylostella prophenoloxidase-1 gene) and PxPPO2 (P. xylostella prophenoloxidase-2 gene) were increased to varying degrees compared with the S strain, such as the transcriptional expression levels of PxPPO2 were 24.02-fold that of the S strain. The responses of phenoloxidase were significantly different in chlorantraniliprole-resistant DBM.

scholarly journals Transcriptome Analysis of Barbarea vulgaris Infested with Diamondback Moth (Plutella xylostella) Larvae

PLoS ONE ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. e64481 ◽  
Author(s):  
Xiaochun Wei ◽  
Xiaohui Zhang ◽  
Di Shen ◽  
Haiping Wang ◽  
Qingjun Wu ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Plutella Xylostella ◽  
Diamondback Moth ◽  
Barbarea Vulgaris

Geographical variation in larval susceptibility of the diamondback moth,Plutella xylostella(Lepidoptera: Plutellidae) toBacillus thuringiensisspore–crystal mixtures and purified crystal proteins and associated resistance development in India

2002 ◽  
Vol 92 (6) ◽  
pp. 489-498 ◽  
Author(s):  
M. Mohan ◽  
G.T. Gujar
Keyword(s):  
Plutella Xylostella ◽  
Tamil Nadu ◽  
Diamondback Moth ◽  
Cross Resistance ◽  
Resistance Development ◽  
Cry Proteins ◽  
Southern State ◽  
Crystal Proteins ◽  
Two Generations ◽  
Different Populations

AbstractThe susceptibility of larvae of the diamondback moth,Plutella xylostellaLinnaeus to purified crystal proteins and spore–crystal preparations ofBacillus thuringiensiswas investigated for 13 populations from seven states in India. The LC50(μg ml−1, 48 h) values of Cry proteins for different populations ofP. xylostellaranged from 0.14–3.74 (Cry1Aa), 0.007–1.25 (Cry1Ab), 0.18–2.47 (Cry1Ac) and 0.12 – 3.0 (Cry1C). The LC50(mg (ai) l-1, 48 h) of spore-crystal preparations ranged from 0.02–0.98 (HD-1) and 0.06–2.14 (HD-73). Significantly higher LC50values for all tested toxins and strains were obtained with populations collected from Iruttupallam and Ottanchathiram in the southern state of Tamil Nadu, whereas some of the populations collected from the northern part of India were more susceptible than the susceptible IARI 17–65 population. The high levels of resistance in the Iruttupallam and Ottanchathiram populations to Cry1Ab suggested selection pressure by Cry1Ab, which is the predominant toxin inB.thuringiensisformulations used in India. Cry1Ab was found to be more toxic than the other toxins. The population from Iruttupallam showed increased resistance following selection with Cry1Ab in the laboratory (LC50from 1.25 to 4.31 μg ml−1over two generations) and also showed cross resistance to Cry1Aa and Cry1Ac. The resistance to Biobit®in the field population from Iruttupallam declined slowly; requiringc. 33 generations for an overall 10-fold decline in LC50when the insects were reared in the laboratory without exposure toB. thuringensis.

Effects of rhizobacteria on the biology and behavior of Plutella xylostella (Lepidoptera: Plutellidae)

2017 ◽  
Vol 43 (2) ◽  
pp. 195
Author(s):  
Robson Thomaz Thuler ◽  
Fernando Henrique Iost Filho ◽  
Hamilton César De Oliveira Charlo ◽  
Sergio Antônio De Bortoli
Keyword(s):  
Plutella Xylostella ◽  
Diamondback Moth ◽  
Insect Behavior ◽  
Control Treatment ◽  
Pupal Weight ◽  
Larval Feeding ◽  
Direct Effects ◽  
Plant Feeding ◽  
Kluyvera Ascorbata ◽  
And Behavior

Plant induced resistance is a tool for integrated pest management, aimed at increasing plant defense against stress, which is compatible with other techniques. Rhizobacteria act in the plant through metabolic changes and may have direct effects on plant-feeding insects. The objective of this study was to determine the effects of cabbage plants inoculated with rhizobacteria on the biology and behavior of diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae). Cabbage seeds inoculated with 12 rhizobacteria strains were sowed in polystyrene trays and later transplanted into the greenhouse. The cabbage plants with sufficient size to support stress were then infested with diamondback moth caterpillars. Later, healthy leaves suffering injuries were collected and taken to the laboratory to feed P. xylostella second instar caterpillars that were evaluated for larval and pupal viability and duration, pupal weight, and sex ratio. The reduction of leaf area was then calculated as a measure of the amount of larval feeding. Non-preference for feeding and oviposition assays were also performed, by comparing the control treatment and plants inoculated with different rhizobacterial strains. Plants inoculated with the strains EN4 of Kluyvera ascorbata and HPF14 of Bacillus thuringiensis negatively affected the biological characteristics of P. xylostella when such traits were evaluated together, without directly affecting the insect behavior.

Mutation of P‐element somatic inhibitor induces male sterility in the diamondback moth, Plutella xylostella

2021 ◽  
Author(s):  
Yaohui Wang ◽  
Xia Xu ◽  
Xi’en Chen ◽  
Xiaowei Li ◽  
Honglun Bi ◽  
...  
Keyword(s):  
Male Sterility ◽  
Plutella Xylostella ◽  
Diamondback Moth ◽  
P Element

scholarly journals On the virulence of the entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae (Ascomycota: Hypocreales), against the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae)

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Muhammad Shehzad ◽  
Muhammad Tariq ◽  
Tariq Mukhtar ◽  
Asim Gulzar
Keyword(s):  
Sex Ratio ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Plutella Xylostella ◽  
Metarhizium Anisopliae ◽  
Insect Pest ◽  
Diamondback Moth ◽  
Economic Damage ◽  
Offspring Sex Ratio ◽  
Development Of Resistance

Abstract Background The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a noxious pest of cruciferous crops all over the world causing serious economic damage. Management of insect pest generally depends on chemical control; however, due to development of resistance against all types of insecticides, alternative approaches especially utilization of a microbial agent is inevitable. Results Potential of 2 entomopathogenic fungi (EPF), viz., Beauveria bassiana and Metarhizium anisopliae, was evaluated against 2nd and 3rd larval instars of P. xylostella by adopting leaf dip and direct spraying methods under laboratory conditions. Significant mortality rate was achieved by each fungus under adopted methodologies. However, B. bassiana was found to be more effective in both conditions than M. anisopliae. Highest mean corrected mortality (77.80%) was recorded, when spores of B. bassiana were sprayed on the 2nd instar larvae (LC50=1.78×104/ml) after the 6th day of treatment. Similarly, incase of M. anisopliae LC50 for the 2nd instar at the same methodology was 2.78×104/ml with a mortality percentage of 70.0%. Offspring sex ratio was non-significantly related to treatment concentration and methodology, except for the control. Conclusion Beauveria bassiana and M. anisopliae had potential to suppress P. xylostella infestations when applied appropriately. Present findings suggested that B. bassiana and M. anisopliae when sprayed on immatures of host insect had more effect as compared to leaf dip procedure. Furthermore, no significant effect of concentrations was observed on sex ratio.

Sign in / Sign up

Export Citation Format

Share Document