Effects of Abscisic Acid, Gibberellin, Ethylene and Their Interactions on Production of Phenolic Acids in Salvia miltiorrhiza Bunge Hairy Roots

Research on the mineral fertilization of Salvia miltiorrhiza Bunge was carried out in the years 2000 - 2002. It was stated that the potassium fertilization has the strongest effect on the content of phenolic acids and on mass of roots of Salvia miltiorrhiza. The effect of atmospheric conditions in particular years is also essential. The optimal doses of fertilizers (pure component content in kg · ha-) were the following: N - 100, P2O5 - 30, K20 - 180. Having used this fertilizing combination the average content of phenolic acids was 4,62% and the average aerial dry mass of roots was 13,21 g per 1 plant after one year of cultivation.

Download Full-text

Quantitative determination and validation of four phenolic acids in Salvia Miltiorrhiza Bunge using 1H-NMR spectroscopy

Current Pharmaceutical Analysis ◽

10.2174/1573412916666191231104909 ◽

2019 ◽

Vol 16 ◽

Cited By ~ 1

Author(s):

Zhuoni Hou ◽

Zongsuo Liang ◽

Yuanyuan Li ◽

Feng Su ◽

Jipeng Chen ◽

...

Keyword(s):

Nmr Spectroscopy ◽

1H Nmr ◽

Phenolic Acids ◽

Rosmarinic Acid ◽

1H Nmr Spectroscopy ◽

Salvia Miltiorrhiza ◽

Salvianolic Acid B ◽

Salvia Miltiorrhiza Bunge ◽

Salvianolic Acid ◽

Root Extracts

Background: Although chromatography and spectrometry based methods have been used to analyse phenolic acids in Chinese traditional medicine Salvia miltiorrhiza Bunge (SMB), quantitative nuclear magnetic resonance (qNMR) has never previously been used to analyse fresh SMB root extracts. Objective: To establish a fast and simple method of quantitating danshensu, lithospermic acid, rosmarinic acid and salvianolic acid B content in fresh SMB root using 1H-NMR spectroscopy. Method: Fresh SMB root were extracted using a 70% methanol aqueous solution and quantitatively analysed for danshensu, lithospermic acid, rosmarinic acid and salvianolic acid B using 1H-NMR spectroscopy. Different internal standards were compared and the results were validated using high performance liquid chromatography. Results: The established method was accurate and precise with good recovery. The LOD and LOQ indicated the excellent sensitivity of the method. The robustness was testified by modification of four different parameters, and the differences among each parameter were all less than 2%. Conclusion: qNMR offers a fast, reliable and accurate method of identifying and quantifying danshensu, lithospermic acid, rosmarinic acid and salvianolic acid B in fresh SMB root extracts.

Download Full-text

Downregulation of Cinnamoyl CoA Reductase Affects Lignin and Phenolic Acids Biosynthesis in Salvia miltiorrhiza Bunge

Plant Molecular Biology Reporter ◽

10.1007/s11105-012-0444-4 ◽

2012 ◽

Vol 30 (5) ◽

pp. 1229-1236 ◽

Cited By ~ 18

Author(s):

Zhengjun Wang ◽

Langjun Cui ◽

Chen Chen ◽

Xiaojing Liu ◽

Yaping Yan ◽

...

Keyword(s):

Phenolic Acids ◽

Salvia Miltiorrhiza ◽

Salvia Miltiorrhiza Bunge ◽

Cinnamoyl Coa Reductase ◽

Coa Reductase

Download Full-text

Regulation of folic acid on phenolic acids production in Salvia miltiorrhiza hairy roots

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-016-1040-3 ◽

2016 ◽

Vol 127 (1) ◽

pp. 175-185 ◽

Cited By ~ 9

Author(s):

Dongfeng Yang ◽

Zhicheng Huang ◽

Bingcong Xing ◽

Weibo Jin ◽

Xijun Yan ◽

...

Keyword(s):

Folic Acid ◽

Phenolic Acids ◽

Hairy Roots ◽

Salvia Miltiorrhiza

Download Full-text

SmMYB98b positive regulation to tanshinones in Salvia miltiorrhiza Bunge hairy roots

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-019-01716-1 ◽

2019 ◽

Vol 140 (2) ◽

pp. 459-467 ◽

Cited By ~ 2

Author(s):

Lin Liu ◽

Dongfeng Yang ◽

Bingcong Xing ◽

Chenlu Zhang ◽

Zongsuo Liang

Keyword(s):

Hairy Roots ◽

Salvia Miltiorrhiza ◽

Salvia Miltiorrhiza Bunge ◽

Positive Regulation

Download Full-text

SmbHLH3 acts as a transcription repressor for both phenolic acids and tanshinone biosynthesis in Salvia miltiorrhiza hairy roots

Phytochemistry ◽

10.1016/j.phytochem.2019.112183 ◽

2020 ◽

Vol 169 ◽

pp. 112183 ◽

Cited By ~ 2

Author(s):

Chenlu Zhang ◽

Bingcong Xing ◽

Dongfeng Yang ◽

Min Ren ◽

Hui Guo ◽

...

Keyword(s):

Phenolic Acids ◽

Hairy Roots ◽

Salvia Miltiorrhiza

Download Full-text

Phosphate starvation promoted the accumulation of phenolic acids by inducing the key enzyme genes in Salvia miltiorrhiza hairy roots

Plant Cell Reports ◽

10.1007/s00299-016-2007-x ◽

2016 ◽

Vol 35 (9) ◽

pp. 1933-1942 ◽

Cited By ~ 15

Author(s):

Lin Liu ◽

DongFeng Yang ◽

TongYao Liang ◽

HaiHua Zhang ◽

ZhiGui He ◽

...

Keyword(s):

Phenolic Acids ◽

Hairy Roots ◽

Salvia Miltiorrhiza ◽

Phosphate Starvation ◽

Key Enzyme

Download Full-text

Systematic Analysis of Kelch Repeat F-box (KFB) Protein Family and Identification of Phenolic Acid Regulation Members in Salvia miltiorrhiza Bunge

Genes ◽

10.3390/genes11050557 ◽

2020 ◽

Vol 11 (5) ◽

pp. 557

Author(s):

Haizheng Yu ◽

Mengdan Jiang ◽

Bingcong Xing ◽

Lijun Liang ◽

Bingxue Zhang ◽

...

Keyword(s):

Secondary Metabolism ◽

Phenolic Acids ◽

Salvia Miltiorrhiza ◽

Chinese Herb ◽

Cerebrovascular Diseases ◽

Regulation Mechanism ◽

Salvia Miltiorrhiza Bunge ◽

Specific Expression ◽

Systematic Analysis ◽

Meja Treatment

S. miltiorrhiza is a well-known Chinese herb for the clinical treatment of cardiovascular and cerebrovascular diseases. Tanshinones and phenolic acids are the major secondary metabolites and significant pharmacological constituents of this plant. Kelch repeat F-box (KFB) proteins play important roles in plant secondary metabolism, but their regulation mechanism in S. miltiorrhiza has not been characterized. In this study, we systematically characterized the S. miltiorrhiza KFB gene family. In total, 31 SmKFB genes were isolated from S. miltiorrhiza. Phylogenetic analysis of those SmKFBs indicated that 31 SmKFBs can be divided into four groups. Thereinto, five SmKFBs (SmKFB1, 2, 3, 5, and 28) shared high homology with other plant KFBs which have been described to be regulators of secondary metabolism. The expression profile of SmKFBs under methyl jasmonate (MeJA) treatment deciphered that six SmKFBs (SmKFB1, 2, 5, 6, 11, and 15) were significantly downregulated, and two SmKFBs (SmKFB22 and 31) were significantly upregulated. Tissue-specific expression analysis found that four SmKFBs (SmKFB4, 11, 16, and 17) were expressed preferentially in aerial tissues, while two SmKFBs (SmKFB5, 25) were predominantly expressed in roots. Through a systematic analysis, we speculated that SmKFB1, 2, and 5 are potentially involved in phenolic acids biosynthesis.

Download Full-text