Functional Characterization of the Poplar R2R3-MYB Transcription Factor PtoMYB216 Involved in the Regulation of Lignin Biosynthesis during Wood Formation

Abstract Background MYB transcription factors, comprising one of the largest transcription factor families in plants, play many roles in secondary metabolism, especially in anthocyanin biosynthesis. However, the functions of the PdeMYB transcription factor in colored-leaf poplar remain elusive. Results In the present study, genome-wide characterization of the PdeMYB genes in colored-leaf poplar (Populus deltoids) was conducted. A total of 302 PdeMYB transcription factors were identified, including 183 R2R3-MYB, five R1R2R3-MYB, one 4R-MYB, and 113 1R-MYB transcription factor genes. Genomic localization and paralogs of PdeMYB genes mapped 289 genes on 19 chromosomes, with collinearity relationships among genes. The conserved domain, gene structure, and evolutionary relationships of the PdeMYB genes were also established and analyzed. The expression levels of PdeMYB genes were obtained from previous data in green leaf poplar (L2025) and colored leaf poplar (QHP) as well as our own qRT-PCR analysis data in green leaf poplar (L2025) and colored leaf poplar (CHP), which provide valuable clues for further functional characterization of PdeMYB genes. Conclusions The above results provide not only comprehensive insights into the structure and functions of PdeMYB genes but also provide candidate genes for the future improvement of leaf colorization in Populus deltoids.

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Isolation and characterization of a R2R3-MYB transcription factor gene related to anthocyanin biosynthesis in the spathes of Anthurium andraeanum (Hort.)

Plant Cell Reports ◽

10.1007/s00299-016-2025-8 ◽

2016 ◽

Vol 35 (10) ◽

pp. 2151-2165 ◽

Cited By ~ 25

Author(s):

Chonghui Li ◽

Jian Qiu ◽

Guangsui Yang ◽

Surong Huang ◽

Junmei Yin

Keyword(s):

Transcription Factor ◽

Anthocyanin Biosynthesis ◽

Myb Transcription Factor ◽

Transcription Factor Gene ◽

Factor Gene ◽

Isolation And Characterization ◽

Anthurium Andraeanum ◽

R2r3 Myb

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Identification and Functional Characterization of PtoMYB055 Involved in the Regulation of the Lignin Biosynthesis Pathway in Populus tomentosa

International Journal of Molecular Sciences ◽

10.3390/ijms21144857 ◽

2020 ◽

Vol 21 (14) ◽

pp. 4857

Author(s):

Yiming Sun ◽

Sha Ren ◽

Shenglong Ye ◽

Qiaoyan Tian ◽

Keming Luo

Keyword(s):

Cell Wall ◽

Vascular Plants ◽

Secondary Cell Wall ◽

Functional Characterization ◽

Lignin Biosynthesis ◽

Transcriptional Activator ◽

Populus Tomentosa ◽

Myb Transcription Factor ◽

Phloem Tissue

Wood, which is mainly composed of lignified secondary cell wall, is the most abundant biomass in woody plants. Previous studies have revealed that R2R3-type MYB transcription factors are important regulators of the formation of the secondary cell wall in vascular plants. In this study, we isolated the R2R3-type MYB transcription factor gene PtoMYB055, which is mainly expressed in xylem and phloem tissue, from Populus tomentosa and demonstrate that PtoMYB055 is a key regulator of lignin biosynthesis. PtoMYB055 as a transcriptional activator is localized to the nucleus. Overexpression of PtoMYB055 upregulates expression of lignin biosynthetic genes in transgenic poplar plants, resulting in ectopic deposition of lignin in phloem tissue and an increase in thickness of the secondary cell wall. In sum, PtoMYB055 is a transcriptional activator that is involved in regulating lignin biosynthesis during the formation of the secondary cell wall in poplar.

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