scholarly journals Inhibition of Nickel Nanoparticles-Induced Toxicity by Epigallocatechin-3-Gallate in JB6 Cells May Be through Down-Regulation of the MAPK Signaling Pathways

PLoS ONE ◽  
2016 ◽  
Vol 11 (3) ◽  
pp. e0150954 ◽  
Author(s):  
Yuanliang Gu ◽  
Yafei Wang ◽  
Qi Zhou ◽  
Linda Bowman ◽  
Guochuan Mao ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Nickel Nanoparticles ◽  
Mapk Signaling ◽  
Down Regulation ◽  
Jb6 Cells ◽  
Mapk Signaling Pathways

Down-regulation of NTSR3 inhibits cell growth and metastasis, as well as the PI3K–AKT and MAPK signaling pathways in colorectal cancer

2020 ◽  
Vol 98 (5) ◽  
pp. 548-555
Author(s):  
Aihua Liu ◽  
Zhongfu Zuo ◽  
Linlin Liu ◽  
Lihua Liu
Keyword(s):  
Colorectal Cancer ◽  
Cell Growth ◽  
Signaling Pathways ◽  
Cell Apoptosis ◽  
Caspase 3 ◽  
Mapk Signaling ◽  
Adhesion Assay ◽  
Caspase 9 ◽  
Down Regulation ◽  
Mapk Signaling Pathways

Colorectal cancer is a common malignancy. NTS receptor 3 (NTSR3) is known to play an important role in several cancers. This study examined the effects of NTSR3 on cell growth and metastasis in colorectal cancer. Western blot analysis, real-time PCR, immunofluorescence staining, MTT, cell cycle assay, cell apoptosis assay, Hoechst staining, caspase-3 and caspase-9 activity assays, cell adhesion assay, wound healing assay, and a Transwell assay were used in this study. We found that NTSR3 was expressed at relatively high levels in the colorectal cancer cell lines SW620 and SW480. NTSR3 knockdown suppressed cell growth and promoted cell apoptosis. Meanwhile, the protein expression levels of cyclinD1, cyclinE1, CDK4, and p-RB were reduced, and the levels of p-P27, P15, P21, cleaved caspase-3, and cleaved caspase-9 protein were increased. Cell invasiveness and cell migration were reduced with knockdown of NTSR3. In addition, our rescue experiments demonstrated that overexpression of the siRNA-resistant alleles of NTSR3 abrogated the NTSR3-siRNA-mediated effects on cell function. Further, down-regulation of NTSR3 inactivated the PI3K–AKT and MAPK signaling pathways. Collectively, these data demonstrate that knockdown of NTSR3 inhibits cell growth and metastasis, as well as the PI3K–AKT and MAPK signaling pathways in colorectal cancer. Thus, our results indicate that NTSR3 is a potential therapeutic target for treating colorectal cancer.

Acteoside inhibits type Ι allergy through the down-regulation of Ca/NFAT and JNK MAPK signaling pathways in basophilic cells

2013 ◽  
Vol 67 (4) ◽  
pp. 790-798 ◽  
Author(s):  
Hideko Motojima ◽  
Myra O. Villareal ◽  
Rieko Iijima ◽  
Junkyu Han ◽  
Hiroko Isoda
Keyword(s):  
Signaling Pathways ◽  
Mapk Signaling ◽  
Down Regulation ◽  
Mapk Signaling Pathways
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