scholarly journals Transcriptome reprogramming of resistant and susceptible peach genotypes during Xanthomonas arboricola pv. pruni early leaf infection

PLoS ONE ◽  
2018 ◽  
Vol 13 (4) ◽  
pp. e0196590 ◽  
Author(s):  
Fabio Gervasi ◽  
Patrizia Ferrante ◽  
Maria Teresa Dettori ◽  
Marco Scortichini ◽  
Ignazio Verde
Keyword(s):  
Leaf Infection ◽  
Xanthomonas Arboricola

scholarly journals Comparative Genomics of Xanthomonaseuroxanthea and Xanthomonas arboricola pv. juglandis Strains Isolated from a Single Walnut Host Tree

2021 ◽  
Vol 9 (3) ◽  
pp. 624
Author(s):  
Camila Fernandes ◽  
Leonor Martins ◽  
Miguel Teixeira ◽  
Jochen Blom ◽  
Joël F. Pothier ◽  
...  
Keyword(s):  
Extracellular Enzymes ◽  
Walnut Tree ◽  
Size Number ◽  
A Genome ◽  
Type 3 Secretion System ◽  
Xanthomonas Arboricola ◽  
Chromosomal Sequences ◽  
Related Proteins ◽  
Type 3 ◽  
Genome Comparisons

The recent report of distinct Xanthomonas lineages of Xanthomonas arboricola pv. juglandis and Xanthomonas euroxanthea within the same walnut tree revealed that this consortium of walnut-associated Xanthomonas includes both pathogenic and nonpathogenic strains. As the implications of this co-colonization are still poorly understood, in order to unveil niche-specific adaptations, the genomes of three X. euroxanthea strains (CPBF 367, CPBF 424T, and CPBF 426) and of an X. arboricola pv. juglandis strain (CPBF 427) isolated from a single walnut tree in Loures (Portugal) were sequenced with two different technologies, Illumina and Nanopore, to provide consistent single scaffold chromosomal sequences. General genomic features showed that CPBF 427 has a genome similar to other X. arboricola pv. juglandis strains, regarding its size, number, and content of CDSs, while X. euroxanthea strains show a reduction regarding these features comparatively to X. arboricola pv. juglandis strains. Whole genome comparisons revealed remarkable genomic differences between X. arboricola pv. juglandis and X. euroxanthea strains, which translates into different pathogenicity and virulence features, namely regarding type 3 secretion system and its effectors and other secretory systems, chemotaxis-related proteins, and extracellular enzymes. Altogether, the distinct genomic repertoire of X. euroxanthea may be particularly useful to address pathogenicity emergence and evolution in walnut-associated Xanthomonas.

scholarly journals Assessment of Xanthomonas arboricola pv. juglandis Bacterial Load in Infected Walnut Fruits by Quantitative PCR

Plant Disease ◽  
2019 ◽  
Vol 103 (10) ◽  
pp. 2577-2586
Author(s):  
Leonor Martins ◽  
Camila Fernandes ◽  
Pedro Albuquerque ◽  
Fernando Tavares
Keyword(s):  
Quantitative Pcr ◽  
Juglans Regia ◽  
Bacterial Load ◽  
Etiologic Agent ◽  
Economic Losses ◽  
Rapid Diagnostics ◽  
In Planta ◽  
Chromosomal Dna ◽  
Fruit Samples ◽  
Xanthomonas Arboricola

Xanthomonas arboricola pv. juglandis is the etiologic agent of important walnut (Juglans regia L.) diseases, causing severe fruit drop and high economic losses in walnut production regions. Rapid diagnostics and knowledge of bacterial virulence fitness are key to hinder disease progression and apply timely phytosanitary measures. This work describes an X. arboricola pv. juglandis-specific real-time quantitative PCR (qPCR) using X. arboricola pv. juglandis-specific DNA markers to quantify the bacterial load in infected walnut plant tissues. Method validation was achieved using calibration curves obtained with serial dilutions of X. arboricola pv. juglandis chromosomal DNA and standard curves obtained from walnut samples spiked with X. arboricola pv. juglandis cells. High correlations (R2 > 0.990 and > 0.995) and low limits of detection (35 chromosomes/qPCR reaction and 2.7 CFU/qPCR reaction) were obtained for both markers considering the calibration and standard curves, respectively. Assessment of qPCR repeatability, reproducibility, and specificity allowed us to demonstrate the reliability and consistency of the method. Furthermore, in planta quantification of X. arboricola pv. juglandis bacterial load using infected walnut fruit samples showed a higher detection resolution compared with standard PCR detection. By allowing quantification of virulence fitness of distinct X. arboricola pv. juglandis strains in planta, the proposed qPCR method may contribute to assertive risk assessment of walnut diseases caused by X. arboricola pv. juglandis and ultimately help to improve phytosanitary practices.

scholarly journals Dynamics in the Strawberry Rhizosphere Microbiome in Response to Biochar and Botrytis cinerea Leaf Infection

2016 ◽  
Vol 7 ◽  
Author(s):  
Caroline De Tender ◽  
Annelies Haegeman ◽  
Bart Vandecasteele ◽  
Lieven Clement ◽  
Pieter Cremelie ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Rhizosphere Microbiome ◽  
Leaf Infection

scholarly journals Lateral flow immunoassay for on-site detection of Xanthomonas arboricola pv. pruni in symptomatic field samples

PLoS ONE ◽  
2017 ◽  
Vol 12 (4) ◽  
pp. e0176201 ◽  
Author(s):  
Pablo López-Soriano ◽  
Patricia Noguera ◽  
María Teresa Gorris ◽  
Rosa Puchades ◽  
Ángel Maquieira ◽  
...  
Keyword(s):  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Field Samples ◽  
Xanthomonas Arboricola

scholarly journals Development of a Bio-PCR Protocol for the Detection of Xanthomonas arboricola pv. pruni

Plant Disease ◽  
2011 ◽  
Vol 95 (9) ◽  
pp. 1109-1115 ◽  
Author(s):  
E. L. Ballard ◽  
R. G. Dietzgen ◽  
L. I. Sly ◽  
C. Gouk ◽  
C. Horlock ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Field Evaluation ◽  
Subtractive Hybridization ◽  
Epiphytic Bacteria ◽  
Supportive Evidence ◽  
Chain Reaction ◽  
Dna Library ◽  
Xanthomonas Arboricola ◽  
Polymerase Chain

A real-time SYBR Green I assay was developed and evaluated as a biological and enzymatic polymerase chain reaction (Bio-PCR) protocol for the detection of Xanthomonas arboricola pv. pruni. Suppression subtractive hybridization was used to generate a X. arboricola pv. pruni-specific subtracted DNA library, using X. arboricola pv. corylina as the driver strain. Primer pair 29F/R, designed from cloned sequence, showed no homology to GenBank sequences and amplified a 344-bp product in all X. arboricola pv. pruni isolates. Compared with other published X. arboricola pv. pruni primers, this primer pair was shown to be the only one capable of differentiating X. arboricola pv. pruni from all other X. arboricola pathovars. A real-time assay was developed and shown to be capable of detecting less than 10 CFU and 0.1 pg of DNA. Epiphytic bacteria isolated from plum tissue was used to further evaluate the specificity of the assay. A Bio-PCR protocol, developed for field evaluation, confirmed X. arboricola pv. pruni isolation from asymptomatic and symptomatic plum tissue over a 9-week period between host flowering and the first appearance of leaf and fruit symptoms in an orchard. Dilution plating enabled X. arboricola pv. pruni numbers to be quantified, providing supportive evidence for the usefulness of the Bio-PCR protocol in plant pathology and quarantine surveillance.

Influence of benzimidazole fungicides on incidence of Botrytis allii infection of onion leaves and subsequent incidence of onion neck rot in storage in Tasmania, Australia

2006 ◽  
Vol 46 (12) ◽  
pp. 1661 ◽  
Author(s):  
M. I. Chilvers ◽  
F. S. Hay ◽  
J. Hills ◽  
J. J. C. Dennis ◽  
C. R. Wilson
Keyword(s):  
Field Trial ◽  
Block Design ◽  
Benzimidazole Fungicides ◽  
Leaf Infection ◽  
Botrytis Allii ◽  
The Mean

Neck rot of onion caused by Botrytis spp., including B. allii, has previously been controlled in Australia with the fungicide Benlate (benomyl). Production of Benlate has recently ceased, therefore a field trial was conducted to examine the efficacy of alternative benzimidazoles fungicides. The trial compared 2 carbendazim fungicides (Marvel and Spin Flo) at 3 rates of application with Benlate applied at commercial rates and to non-treated plots in a randomised complete block design with 4 replicate plots per treatment. Fungicides were applied at 89, 96, 112 and 119 days after sowing. Plots were sprayed with inoculum consisting of a suspension of B. allii conidia 103 days after sowing. The incidence of B. allii infection in leaves was estimated 10 times during the season by collection and incubation of leaf samples. Fifty-six days after inoculum application the mean incidence of B. allii in leaves from fungicide treatments ranged from 0 to 10%, significantly lower (P<0.05) than that of non-treated plots (28.8%). The mean incidence of neck rot in bulb samples after 3 months of storage ranged from 1.0 to 9.9% in fungicide treatments, significantly (P<0.05) lower than that of non-treated plots (63.4%). The incidence of B. allii leaf infection in plots sampled at different times during the season and the incidence of neck rot in storage were all significantly correlated (r = 0.42–0.61, P<0.01), except prior to application of inoculum.

Multilocus Sequence Analysis and Copper Ion Resistance Detection of 60 Xanthomonas arboricola pv. juglandis Isolates from China

Plant Disease ◽  
2021 ◽  
Author(s):  
Benzhong Fu ◽  
Jieqian Zhu ◽  
Conard Lee ◽  
Lihua Wang
Keyword(s):  
Sequence Analysis ◽  
Copper Ion ◽  
Housekeeping Genes ◽  
Threshold Value ◽  
Pcr Primers ◽  
Multilocus Sequence Analysis ◽  
Nucleotide Polymorphisms ◽  
Specific Pcr ◽  
Resistant Strains ◽  
Xanthomonas Arboricola

Walnut bacterial blight caused by Xanthomonas arboricola pv. juglandis (Xaj) has serious repercussions for walnut production around the world. Between 2015 and 2017, disease samples were collected from six counties (Danjiangkou, Baokang, Suizhou, Shennongjia, Zigui, and Xingshan) in Hubei province, China. Fifty-nine Xaj strains were identified by morphology and specific PCR primers from 206 isolates. The genetic diversity of 60 Xaj strains (59 from Hubei plus one from Beijing) was evaluated by Multilocus Sequence Analysis (MLST), and their resistance to copper ion (Cu2+) treatment was determined. A Neighbor Joining phylogenetic dendrogram was constructed based on four sequences of housekeeping genes (atpD-dnaK-glnA-gyrB). Two groups of strains were identified whose clustering was consistent with that of glnA. The minimal inhibitory concentration of copper ion on representative Xaj strain DW3F3 (the first genome sequenced Xaj from China) was 115 μg/ml. Setting the copper resistant threshold value to 125 μg/ml, 47 and 13 strains were considered sensitive and resistant to Cu2+, respectively. Furthermore, five strains showed Cu2+ resistance at 270 μg/ml. Compared to the copB from sensitive strains, the copB gene in resistant strains had a 15-bp insertion and eight scattered single nucleotide polymorphisms. Interestingly, the clustering based on MLSA was distinct between Xaj copper ion resistant and sensitive strains.

scholarly journals Heritability of peach tree resistance to bacterial leaf spot

2017 ◽  
Vol 52 (5) ◽  
pp. 366-369 ◽  
Author(s):  
André Luiz Varago ◽  
Idemir Citadin ◽  
Marcos Robson Sachet ◽  
Gener Augusto Penso ◽  
Maria do Carmo Bassols Raseira
Keyword(s):  
Leaf Area ◽  
Disease Severity ◽  
Leaf Spot ◽  
Field Conditions ◽  
Broad Sense Heritability ◽  
Peach Tree ◽  
Bacterial Leaf Spot ◽  
Tree Resistance ◽  
Leaf Area Duration ◽  
Xanthomonas Arboricola

Abstract: The objective of this work was to evaluate the broad-sense heritability reaction to bacterial leaf spot (Xanthomonas arboricola pv. pruni), in peach tree populations obtained from directed crosses. Disease severity and defoliation of the genotypes were evaluated in field conditions, with posterior measurement of the healthy leaf area duration (HAD). The observed average heritability (0.51) indicates that the use of the evaluated genitors can be effective for the development of cultivars with higher resistance to the disease.

scholarly journals Genome Sequence of Xanthomonas arboricola pv. Corylina, Isolated from Turkish Filbert in Colorado

2013 ◽  
Vol 1 (3) ◽  
Author(s):  
J. Ibarra Caballero ◽  
M. M. Zerillo ◽  
J. Snelling ◽  
C. Boucher ◽  
N. Tisserat
Keyword(s):  
Genome Sequence ◽  
Xanthomonas Arboricola
Sign in / Sign up

Export Citation Format

Share Document