scholarly journals Molecular phylogeny and bioprospecting of Endolichenic Fungi (ELF) inhabiting in the lichens collected from a mangrove ecosystem in Sri Lanka

PLoS ONE ◽  
2018 ◽  
Vol 13 (8) ◽  
pp. e0200711 ◽  
Author(s):  
Kasun Maduranga ◽  
Renuka Nilmini Attanayake ◽  
Sinthujah Santhirasegaram ◽  
Gothamie Weerakoon ◽  
Priyani Ashoka Paranagama
Keyword(s):  
Sri Lanka ◽  
Molecular Phylogeny ◽  
Mangrove Ecosystem ◽  
Endolichenic Fungi

scholarly journals Bioactive Properties and Metabolite Profiles of Endolichenic Fungi in Mangrove Ecosystem of Negombo Lagoon, Sri Lanka

2021 ◽  
Vol 16 (10) ◽  
pp. 1934578X2110486
Author(s):  
Ramani H. Weerasinghe ◽  
Kasun Maduranga ◽  
Renuka N. Attanayake ◽  
Chaitrali Shevkar ◽  
Abhijeet S. Kate ◽  
...  
Keyword(s):  
Sri Lanka ◽  
Radical Scavenging Activity ◽  
Bacterial Species ◽  
Radical Scavenging ◽  
Mangrove Ecosystem ◽  
In Vitro Assays ◽  
Curvularia Lunata ◽  
Endolichenic Fungi ◽  
Anti Inflammatory

Endolichenic fungi (ELF) serve as a novel source of natural products with distinctive bioactivities. A total of 58 ELF isolated from 29 lichens collected from mangrove and mangrove-associated plants of Negombo lagoon, Sri Lanka were identified using morphological and DNA barcoding techniques. Ethyl acetate extracts of 18 such identified ELF isolates were subjected to in vitro assays to determine antioxidant, anti-inflammatory, tyrosinase inhibitory and antibacterial potency. Liquid chromatography–mass spectrometry (LC–MS) dereplication was conducted on the crude extracts in order to detect the secondary metabolites present. The extracts of Daldinia eschscholtzii and Hypoxylon lividipigmentum had the highest radical scavenging activity with SC50 values 14.27 ± 0.24 µg/mL and 18.34 ± 1.37 µg/mL, respectively. D. eschscholtzii also exhibited remarkable anti-inflammatory activity (IC50 7.97 ± 0.09 µg/mL). Tyrosinase inhibitory activity was highest in Cytospora xylocarpi (IC50 68.50 ± 0.34 µg/mL), while the highest activity against aerobic bacterial species Escherichia coli, Bacillus subtilis, Staphylococcus aureus and the anaerobic bacterial strain Streptococcus mutans was observed in the extracts of Xylaria feegenesis and Curvularia lunata. After a thorough study of the LC–MS profiles, it was found that the chemical profiles of Neofusicoccum occulatum, H. lividipigmentum and Myramaececium rubricosum were previously poorly explored in the literature.

scholarly journals Identification of Novel Bioactive Compounds, Neurosporalol 1 and 2 from an Endolichenic Fungus, Neurospora ugadawe Inhabited in the Lichen Host, Graphis tsunodae Zahlbr. from Mangrove Ecosystem in Puttalam Lagoon, Sri Lanka

2021 ◽  
Vol 33 (6) ◽  
pp. 1425-1432
Author(s):  
H.A.K. Maduranga ◽  
W.R.H. Weerasinghe ◽  
R.N. Attanayake ◽  
S. Santhirasegaram ◽  
C.D. Shevkar ◽  
...  
Keyword(s):  
Sri Lanka ◽  
Secondary Metabolites ◽  
Mangrove Ecosystem ◽  
Positive Control ◽  
Mangrove Ecosystems ◽  
Pure Compounds ◽  
Endolichenic Fungus ◽  
Endolichenic Fungi ◽  
Anti Inflammatory ◽  
Promising Source

Endolichenic fungi (ELF) have been recognized as a promising source of bioactive secondary metabolites. Sri Lanka, a biodiversity hotspot, harbours a remarkable diversity in mangrove ecosystems. In the present study, endolichenic fungi obtained from mangrove lichens of Puttalam lagoon were studied for their secondary metabolites. An endolichenic fungus, Neurospora ugadawe, isolated from the lichen host Graphis tsunodae Zahlbr. was identified up to the species level. Secondary metabolites of N. ugadawe were extracted into ethyl acetate and crude extract showed promising antioxidant and anti-inflammatory activities. Bioassay guided separation on silica gel column chromatography and preparative TLC were performed to isolate the bioactive pure compounds. Two novel bioactive pure compounds were identified as Neurosporalol 1 and Neurosporalol 2. Neurosporalol 1 showed the highest antioxidant activity compared with the positive control BHT. Further, this compound showed very low anti-inflammatory activity compared to that of the positive control aspirin. Neurosporalol 2 showed comparable antioxidant and moderate anti-inflammatory activities.

Molecular phylogeny-based identification of Colletotrichum endophytica and C. siamense as causal agents of avocado anthracnose in Sri Lanka

2021 ◽  
Vol 50 (4) ◽  
pp. 449
Author(s):  
D. M. S. Dissanayake ◽  
N. K. B. Adikaram ◽  
D. M. D. Yakandawala ◽  
L. Jayasinghe
Keyword(s):  
Sri Lanka ◽  
Molecular Phylogeny

Taxonomy and molecular phylogeny of the Platystictidae of Sri Lanka (Insecta: Odonata)

Zootaxa ◽  
2016 ◽  
Vol 4182 (1) ◽  
pp. 1 ◽  
Author(s):  
M. BEDJANIČ ◽  
K. CONNIFF ◽  
R.A. DOW ◽  
F.R. STOKVIS ◽  
R. VEROVNIK ◽  
...  
Keyword(s):  
Sri Lanka ◽  
Molecular Phylogeny

scholarly journals Genetic Diversity and Molecular Phylogeny of Cyanobacteria from Sri Lanka Based on 16S rRNA Gene

2014 ◽  
Vol 19 (4) ◽  
pp. 317-329 ◽  
Author(s):  
R.P. Wanigatunge ◽  
D.N. Magana-Arachchi ◽  
N.V. Chandrasekharan ◽  
S.A. Kulasooriya
Keyword(s):  
Genetic Diversity ◽  
Sri Lanka ◽  
16S Rrna ◽  
Molecular Phylogeny ◽  
16S Rrna Gene ◽  
Rrna Gene

scholarly journals Molecular phylogeny helps to delimit Plectranthus hadiensis from its related morph occurring in Sri Lanka

2019 ◽  
Vol 48 (2) ◽  
pp. 133 ◽  
Author(s):  
Jacqueline Heckenhauer ◽  
Dushyantha Large ◽  
Rosabelle Samuel ◽  
Michael H. J. Barfuss ◽  
Pieter D. H. Prins
Keyword(s):  
Sri Lanka ◽  
Molecular Phylogeny

A molecular phylogeny of the freshwater‐fish genus Rasbora (Teleostei: Cyprinidae) in Sri Lanka reveals a remarkable diversification—And a cryptic species

2020 ◽  
Vol 58 (4) ◽  
pp. 1076-1110 ◽  
Author(s):  
Hiranya Sudasinghe ◽  
Rohan Pethiyagoda ◽  
Ranasinghe Hettiarachchige Tharindu Ranasinghe ◽  
Rajeev Raghavan ◽  
Neelesh Dahanukar ◽  
...  
Keyword(s):  
Sri Lanka ◽  
Molecular Phylogeny ◽  
Cryptic Species ◽  
Freshwater Fish
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