Ion release and recharge from a fissure sealant containing amorphous calcium phosphate

To assess- the release of calcium and phosphate ions from a fissure sealant containing amorphous calcium phosphate (ACP), and to determine the re-release capacity of these ions when charged with a solution containing casein phosphopeptide-amorphous calcium phosphate (CPP-ACP). Nine blocks of ACP resin-based sealant were prepared and immersed in three solutions at different pH (4.0, 5.5, 7.0), and calcium and phosphate ion release was measured with ion chromatography at 1, 3, 5, 7, 14, 21 and 28 days after immersion. Sixty days after immersion, each block was charged with CPP-ACP solution in three 7-day cycles to investigate the re-release of these ions, which was measured on days 1, 3, and 7. No difference was observed in initial calcium ion release at pH 4.0 and pH 5.5. At both values, ion release was significantly higher than at pH 7.0 (p<0.001). Initial phosphate release was significantly different among the three pH values (p<0.001). After re-charging the specimens, calcium ion re-release was greater than phosphate ion release. Initial ion release from ACP resin-based sealant was greatest at the lowest pH. Ion release decreased with time. As the number of recharge cycles increased, ion re-release also improved. Phosphate ion re-release required more recharge cycles than calcium ion re-release.

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THE EFFECT OF CASEIN PHOSPHOPEPTIDE-AMORPHOUS CALCIUM PHOSPHATE-PROPOLIS CHEWING GUM ON CALCIUM AND PHOSPHATE ION LEVELS IN CARIES-FREE SUBJECT’S SALIVA AND STREPTOCOCCUS MUTANS BIOFILM FORMATION

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10s5.23125 ◽

2017 ◽

Vol 10 (17) ◽

pp. 174

Author(s):

Sri Angky Soekanto ◽

Gadiacanaparimita Ghrena Duhita ◽

Endang Winiati Bachtiar ◽

Muhamad Sahlan

Keyword(s):

Calcium Phosphate ◽

Streptococcus Mutans ◽

Calcium Ions ◽

Amorphous Calcium Phosphate ◽

Chewing Gum ◽

Phosphate Ion ◽

Phosphate Ions ◽

Casein Phosphopeptide ◽

Mass Formation

Objective: The aim of this study is to analyze the amount of calcium and phosphate ion released by casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), which supports the remineralization and the effectiveness of propolis wax in suppressing the mass formation of Streptococcus mutans biofilm on caries-free subjects and to observe if these two active components are effective when combined into one substance and placed inside sugar-free chewing gum.Methods: Twenty-five samples of caries-free saliva before and after the mastication simulation (five concentrations of chewing gum) in vitro were observed to check for release of calcium and phosphate ions along with a biofilm assay. The release of calcium ions was detected using AAS, the release of phosphate ions was detected using spectrophotometry ultraviolet-visible, and the biofilm assay was detected using a crystal violet 96-well plate ELISA and was evaluated with an ELISA reader.Result: Chewing gum with a concentration of 0% prop + 5% CPP-ACP showed the highest release level of calcium ions (p<0.05) and phosphate ions (p>0.05) and is significant in suppressing the mass formation of the S. mutans biofilm (p<0.05).Conclusions: Mastication simulation of CPP-ACP-Propolis chewing gum can increase calcium and phosphate ion levels in caries-free saliva and decrease S. mutans biofilm mass formation; this can support remineralization and becomes a dental caries prevention alternative.

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Apatite-Forming Ability of Flowable vs. Putty Formulations of Newly Developed Bioactive Glass-Containing Endodontic Cement

Applied Sciences ◽

10.3390/app11198969 ◽

2021 ◽

Vol 11 (19) ◽

pp. 8969

Author(s):

Naoki Edanami ◽

Razi Saifullah Ibn Belal ◽

Shoji Takenaka ◽

Kunihiko Yoshiba ◽

Nagako Yoshiba ◽

...

Keyword(s):

Calcium Ions ◽

Calcium Ion ◽

Body Fluid ◽

Apatite Formation ◽

Ion Release ◽

Phosphate Ion ◽

Phosphate Ions ◽

Ability Levels ◽

Consumption Rates ◽

The Media

This study compared the apatite-forming ability (AFA) levels of flowable and putty formulations of Nishika Canal Sealer BG Multi (F-NBG and P-NBG, respectively) and attempted to clarify the cause of differences in the AFA levels of F-NBG and P-NBG. NBG samples were aged in simulated body fluid (SBF) or 1-, 5-, or 10-g/L bovine serum albumin-containing SBF (BSA-SBF) and analyzed in terms of their ultrastructures, elemental compositions, and Raman spectra to identify apatite formation. The phosphate ion consumption rates of NBG samples in the media were evaluated as an indicator of apatite growth. The original elemental composition, calcium ion release, and alkalizing ability levels of F-NBG and P-NBG were also evaluated. Apparent apatite formation was detected on all NBG samples except F-NBG aged in 10-g/L BSA-SBF. P-NBG consumed phosphate ions faster than F-NBG. As-prepared P-NBG showed more silicon elements on its surface than as-prepared F-NBG. P-NBG released more calcium ions than F-NBG, although their alkalizing ability levels did not differ statistically. In conclusion, the AFA of P-NBG was greater than that of F-NBG, probably because of the greater ability of P-NBG to expose silanol groups on the surface and release calcium ions.

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Novel multifunctional dental bonding agent for class-V restorations to inhibit periodontal biofilms

RSC Advances ◽

10.1039/c6ra28711e ◽

2017 ◽

Vol 7 (46) ◽

pp. 29004-29014 ◽

Cited By ~ 12

Author(s):

Lin Wang ◽

Chunyan Li ◽

Michael D. Weir ◽

Ke Zhang ◽

Yanmin Zhou ◽

...

Keyword(s):

Calcium Phosphate ◽

Amorphous Calcium Phosphate ◽

Bonding Agent ◽

Ion Release ◽

Class V ◽

Dental Bonding ◽

Phosphate Ion

We recently developed a dental bonding agent to bond restorations to teeth using nanoparticles of amorphous calcium phosphate (NACP) for remineralization with rechargeable calcium and phosphate ion release.

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Effect of the application of a casein phosphopeptide–amorphous calcium phosphate (CPP–ACP) paste and adhesive systems on bond durability of a fissure sealant

Odontology ◽

10.1007/s10266-012-0062-5 ◽

2012 ◽

Vol 101 (1) ◽

pp. 52-59 ◽

Cited By ~ 5

Author(s):

Boniek Castillo Dutra Borges ◽

Anderson Catelan ◽

Robson Tetsuo Sasaki ◽

Gláucia Maria Bovi Ambrosano ◽

André Figueiredo Reis ◽

...

Keyword(s):

Calcium Phosphate ◽

Amorphous Calcium Phosphate ◽

Adhesive Systems ◽

Fissure Sealant ◽

Casein Phosphopeptide ◽

Bond Durability

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Reversal of Root Caries with Casein Phosphopeptide-Amorphous Calcium Phosphate and Fluoride Varnish in Xerostomia

Caries Research ◽

10.1159/000516176 ◽

2021 ◽

pp. 1-10

Author(s):

Ahmed Sleibi ◽

Anwar R. Tappuni ◽

Aylin Baysan

Keyword(s):

Calcium Phosphate ◽

Amorphous Calcium Phosphate ◽

Primary Root ◽

Severity Index ◽

Salivary Flow ◽

Root Caries ◽

Carious Lesions ◽

Group 2 ◽

Casein Phosphopeptide ◽

Group 1

Different formulas of topical fluoride have been used to manage root carious lesions. This clinical trial aimed to investigate the efficacy of a dental varnish containing casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and fluoride compared with fluoride alone in reversing/arresting root caries in xerostomic patients over 1 year. A total of 80 patients (age range 45–92 years) with primary root caries (n = 184 root carious lesions) and unstimulated salivary flow rate of <0.2 mL/min were randomly allocated to receive either dental varnish containing CPP-ACP and 5% fluoride (group 1: MI varnish; GC, Japan) (n = 41, 83 lesions), or dental varnish with 5% fluoride alone (group 2: NUPRO White; Dentsply, USA) (n = 39, 101 lesions). Clinical assessments with Severity Index (SI) for root caries, DIAGNOdent measurements, and varnish application were carried out at baseline, 3, 6, and 12 months. Standard oral hygiene instructions with 1,450 ppm fluoride toothpastes were provided for both groups. After 3 months, 63.9% (n = 46) of root caries in group 1 became hard (SI: 0) compared with 39.3% (n = 35) in group 2 (p < 0.01). After 6 and 12 months, the differences in SI were insignificant (group 1, n = 60, 83.3%) (group 2, n = 66, 74.2%) (p = 0.36), and (group 1, n = 60, 89.6%) (group 2, n = 67, 81.7%, n = 1 soft, 1.2%) (p = 0.29), respectively. In both groups, noncavitated leathery lesions were more likely to become hard when compared to the cavitated root caries. A significant decrease in plaque index, surface roughness, lesion dimension, and DIAGNOdent readings with a significant increase in lesion distance from the gingival margin was reported in both groups (p < 0.05). This study has provided evidence that fluoride dental varnish either with or without calcium and phosphate has the potential to arrest/reverse root caries, especially noncavitated lesions for patients with xerostomia.

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