Effects of transport and altitude on hormones and oxidative stress parameters in sheep

In study, it was aimed to determine the stress effects that can be caused by transporting and altitude in sheep. Karayaka sheeps were used in the study. Karayaka sheeps are a valuable breed of meat quality and fleece, which is raised in the Black Sea region. The live weight of the sheep (n = 30) while hungry was determined before transport and sea level. Average live weight was determined as 55.64 ± 4.66 kg. Blood samples were collected just before (sea level) and just after transport (1500 meters above sea level). Transportation distance was approximately 182 km and duration was 5 hours. According to the findings, cortisol was not affected by transport stress and altitude (P>0.05) and Triiyodotironin (T3) (P<0.039) and Tyrosine (T4) (P<0.000) were affected significantly. Malondialchehyche (MDA), which is one of the oxidative stress parameters, was significantly affected (P<0.039) and Protein Carbonyl (PC) values were not affected by transport and altitude (P>0.184). As a result of this study, it was determined that transportation and altitude in sheep causes stress. Stress-reducing measures should be taken in the exposure of sheep to altitude differences and in transportation. Antioxidant nutritional supplements should be made in order not to negatively affect the meat quality in sheep.

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Effects of Transport and Altitude on Hormones and Oxidative Stress Parameters in Sheep

10.1101/2020.12.21.423758 ◽

2020 ◽

Author(s):

Hilal Tozlu Çelık ◽

Fatih Ahmet Aslan ◽

Diler Us Altay ◽

Metehan Eser Kahvecı ◽

Kalbiye Konanç ◽

...

Keyword(s):

Oxidative Stress ◽

Sea Level ◽

Live Weight ◽

Protein Carbonyl ◽

Stress Effects ◽

Oxidative Stress Parameters ◽

Transport Stress ◽

And Oxidative Stress ◽

Stress Parameters ◽

Made In

AbstractWith this study, it was aimed to determine the stress effects that can be caused by transporting and altitude in sheep. Karayaka sheep were used in the study. The live weight of the sheep (n=30) while hungry was determined before transport and sea level. Average live weight was determined as 55.64 ± 4.66 kg. Blood samples were collected just before (sea level) and just after transport (1500 meters above sea level). Transportation distance was approximately 182 km and duration was 5 hours. According to the findings, cortisol was not affected by transport stress and altitude (p>0.05) and Triiyodotironin (T3) (p<0.039) and Tyrosine (T4) (p<0.000) were affected significantly. Malondialchehyche (MDA), which is one of the oxidative stress parameters, was significantly affected (p<0.039) and Protein Carbonyl (PC) values were not affected by transport and altitude (p>0.184). As a result of this study, it was determined that transportation and altitude in sheep causes stress. Stress-reducing measures should be taken in the exposure of sheep to altitude differences and in transportation. Antioxidant nutritional supplements should be made in order not to negatively affect the meat quality in sheep.

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EFFECTS OF AEROBIC EXERCISE TRAINING ON OXIDATIVE STRESS IN THE SKELETAL MUSCLES OF OBESE RATS

Revista Brasileira de Medicina do Esporte ◽

10.1590/1517-869220192505184278 ◽

2019 ◽

Vol 25 (5) ◽

pp. 404-408

Author(s):

Ariene Sampaio Souza Farias Ulbricht ◽

Daniela Delwing-de Lima ◽

Carla Werlang-Coelho ◽

Débora Delwing-Dal Magro ◽

Bruna Donat ◽

...

Keyword(s):

Oxidative Stress ◽

Soleus Muscle ◽

Skeletal Muscles ◽

Protein Carbonyl ◽

Aerobic Exercise Training ◽

Carbonyl Content ◽

Plantaris Muscle ◽

Protein Carbonyl Content ◽

Oxidative Stress Parameters ◽

Stress Parameters

ABSTRACT Introduction Obesity is a complex and multifactorial metabolic disorder characterized by the accumulation of body fat; physical exercise increases energy expenditure and promotes a reparative effect through modulation of endogenous antioxidant defenses. Objective To evaluate the effects of the high-fat diet (HFD) on oxidative stress parameters in skeletal muscles of rats using aerobic exercise training protocols (AETP), moderate-intensity continuous training (MICT) and high-intensity interval training (HIIT). Methods The study was quantitative and experimental. Animals received 8 weeks of HFD or normal diet (ND), followed by 9 weeks of HFD or ND and the two AETPs. Results HFD did not alter the formation of thiobarbituric acid reactive substances (TBA-RS), total sulfhydryl and protein carbonyl content in the soleus and plantaris muscles; in contrast, the protocols caused a decrease in TBA-RS levels in the plantaris muscle and increased the sulfhydryl content in the soleus muscle, while MICT increased the sulfhydryl content in the plantaris muscle and reduced protein carbonyl content in both muscles. HFD reduced SOD activity in the plantaris muscle while the MICT protocol enhanced SOD in the soleus muscle and both protocols reversed the decrease in SOD in the plantaris muscle. HFD increased CAT activity in the soleus muscle, the HIIT protocol prevented this alteration and both protocols increased CAT in the plantaris muscle. HFD reduced GSH-Px activity in both muscles, and the MICT protocol prevented this reduction in the soleus muscle, while the HIIT protocol partially prevented this decrease. The MICT protocol did not prevent the reduction of GSH-Px and the HIIT protocol partially prevented this decrease in the plantaris muscle. Conclusions HFD elicited oxidative stress in the skeletal muscle of rats, and both protocols were able to prevent most of the alterations in oxidative stress parameters caused by the HFD. Level of evidence IV; Investigation of treatment outcomes.

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An Investigation of Oxidative Stress and Thiol/Disulphide Homeostasis in Graves’ Disease

Medicina ◽

10.3390/medicina55060275 ◽

2019 ◽

Vol 55 (6) ◽

pp. 275 ◽

Cited By ~ 1

Author(s):

Veysel Agan ◽

Hakim Celik ◽

Mehmet Ali Eren ◽

Fatma Zehra Agan ◽

Ozcan Erel ◽

...

Keyword(s):

Oxidative Stress ◽

Protein Carbonyl ◽

Stress Index ◽

Control Group ◽

Graves Disease ◽

Disease Group ◽

Free Triiodothyronine ◽

Oxidative Stress Parameters ◽

Total Thiol ◽

Stress Parameters

Background and objectives: The aim of this study was to research oxidative stress and thiol/disulphide homeostasis in Graves’ patients. Materials and Methods: The study included 33 Graves’ patients (research group) and 35 healthy subjects (control group). Serum oxidative stress and thiol/disulphide homeostasis (a new and automated spectrophotometric method developed by Erel and Neselioglu) parameters were studied and compared between the groups. Results: The native and total thiol levels and the native thiol/total thiol ratio were lower in patients with Graves’ disease compared to the control group (p < 0.001, p < 0.001, and p = 0.006, respectively). TOS (total antioxidant status), PC (protein carbonyl), OSI (Oxidative stress index), and disulphide/native thiol and disulphide/total thiol ratios were determined to be higher in the Graves’ disease group than in the control group (p < 0.001, p = 0.001, p = 0.001, p = 0.004, and p = 0.006, respectively). In the Graves’ disease group, the free triiodothyronine (FT3) and free thyroxine (FT4) levels were significantly positively correlated with impaired thiol/disulphide homeostasis and oxidative stress parameters (p < 0.05). Conclusion: The results of the current study demonstrated that oxidative stress and thiol/disulphide homeostasis increased towards disulphide formation due to thiol oxidation in Graves’ disease. In addition, a positive correlation of FT3 and FT4 was observed with oxidative stress parameters and impaired thiol/disulphide homeostasis.

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