Bone marrow-derived mesenchymal stem cells transplanted into a vascularized biodegradable tube containing decellularized allogenic nerve basal laminae promoted peripheral nerve regeneration; can it be an alternative of autologous nerve graft?

Previously, we showed silicone nerve conduits containing a vascular bundle and decellularized allogenic basal laminae (DABLs) seeded with bone marrow-derived mesenchymal stem cells (BMSCs) demonstrated successful nerve regeneration. Nerve conduits should be flexible and biodegradable for clinical use. In the current study, we used nerve conduits made of polyglycoric acid (PGA) fiber mesh, which is flexible, biodegradable and capillary-permeable. DABLs were created using chemical surfactants to remove almost all cell debris. In part 1, capillary infiltration capability of the PGA tube was examined. Capillary infiltration into regenerated neural tissue was compared between the PGA tube with blood vessels attached extratubularly (extratubularly vascularized tube) and that containing blood vessels intratubularly (intratubularly vascularized tube). No significant difference was found in capillary formation or nerve regeneration between these two tubes. In part 2, a 20 mm gap created in a rat sciatic nerve model was bridged using the extratubularly vascularized PGA tube containing the DABLs with implantation of isogenic cultured BMSCs (TubeC+ group), that containing the DABLs without implantation of the BMSCs (TubeC- group), and 20 mm-long fresh autologous nerve graft (Auto group). Nerve regeneration in these three groups was assessed electrophysiologically and histomorphometrically. At 24 weeks, there was no significant difference in any electrophysiological parameters between TubeC+ and Auto groups, although all histological parameters in Auto group were significantly greater than those in TubeC+ and TubeC- groups, and TubeC+ group demonstrated significant better nerve regeneration than TubeC- group. The transplanted DABLs showed no signs of immunological rejection and some transplanted BMSCs were differentiated into cells with Schwann cell-like phenotype, which might have promoted nerve regeneration within the conduit. This study indicated that the TubeC+ nerve conduit may become an alternative to nerve autograft.

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Use of chitosan conduit combined with bone marrow mesenchymal stem cells for promoting peripheral nerve regeneration

Journal of Materials Science Materials in Medicine ◽

10.1007/s10856-010-4003-y ◽

2010 ◽

Vol 21 (5) ◽

pp. 1713-1720 ◽

Cited By ~ 32

Author(s):

Lei Zheng ◽

Hui-Fei Cui

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Peripheral Nerve ◽

Nerve Regeneration ◽

Peripheral Nerve Regeneration ◽

Marrow Mesenchymal Stem Cells

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Chitin Nerve Conduits with Three-Dimensional Spheroids of Mesenchymal Stem Cells from SD Rats Promote Peripheral Nerve Regeneration

Polymers ◽

10.3390/polym13223957 ◽

2021 ◽

Vol 13 (22) ◽

pp. 3957

Author(s):

Ci Li ◽

Meng Zhang ◽

Song-Yang Liu ◽

Feng-Shi Zhang ◽

Teng Wan ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Peripheral Nerve ◽

Nerve Regeneration ◽

Therapeutic Potential ◽

Peripheral Nerve Regeneration ◽

Nerve Fibers ◽

Therapeutic Effects ◽

Bone Mesenchymal Stem Cells ◽

Nerve Conduits

Peripheral nerve injury (PNI) is an unresolved medical problem with limited therapeutic effects. Epineurium neurorrhaphy is an important method for the treatment of PNI in clinical application, but it is accompanied by inevitable complications such as the misconnection of nerve fibers and neuroma formation. Conduits small gap tubulization has been proved to be an effective suture method to replace the epineurium neurorrhaphy. In this study, a chitin conduit was used to bridge the peripheral nerve stumps. The micromorphology, mechanical property, and biocompatibility of chitin conduits were characterized. The results showed chitin was a high-quality biological material for constructing nerve conduits. In addition, previous reports demonstrated that mesenchymal stem cells culture as spheroids can improve the therapeutic potential. In the present study, we used a hanging drop protocol to prepare bone mesenchymal stem cells (BMSCs) spheroids. Meanwhile, spherical stem cells could express higher stemness-related genes. In the PNI rat models with small gap tubulization, the transformation of BMSCs spheroids, but not BMSCs monolayer, improved sciatic nerve regeneration. Therefore, combining BMSCs spheroids with chitin nerve conduits shows application potential in promoting peripheral nerve regeneration.

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Bone marrow-derived mesenchymal stem cells versus adipose-derived mesenchymal stem cells for peripheral nerve regeneration

Neural Regeneration Research ◽

10.4103/1673-5374.224378 ◽

2018 ◽

Vol 13 (1) ◽

pp. 100 ◽

Cited By ~ 12

Author(s):

Marcela Fernandes ◽

MariaJosé da Silva Fernandes ◽

SandraGomes Valente ◽

RodrigoGuerra Sabongi ◽

JoãoBaptista Gomes dos Santos ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Peripheral Nerve ◽

Nerve Regeneration ◽

Peripheral Nerve Regeneration

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Peripheral nerve regeneration: a comparative study of the effects of autologous bone marrow-derived mesenchymal stem cells, platelet-rich plasma and lateral saphenous vein graft as a conduit in a dog model

Open Veterinary Journal ◽

10.5455/ovj.2021.v11.i4.20 ◽

2021 ◽

Vol 11 (4) ◽

pp. 686

Author(s):

Mousa Daradka ◽

Zuhair Ismail ◽

Mohammad Irsheid

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Peripheral Nerve ◽

Nerve Regeneration ◽

Platelet Rich Plasma ◽

Saphenous Vein Graft ◽

Peripheral Nerve Regeneration ◽

Autologous Bone Marrow ◽

Autologous Bone

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Mesenchymal Stem Cells Derived from Wharton’s Jelly Can Differentiate into Schwann Cell-Like Cells and Promote Peripheral Nerve Regeneration in Acellular Nerve Grafts

Tissue Engineering and Regenerative Medicine ◽

10.1007/s13770-020-00329-6 ◽

2021 ◽

Author(s):

Soon Jin Choi ◽

Suk Young Park ◽

Young Ho Shin ◽

Seung-Ho Heo ◽

Kang-Hyun Kim ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Schwann Cell ◽

Peripheral Nerve ◽

Nerve Regeneration ◽

Peripheral Nerve Regeneration ◽

Wharton’S Jelly ◽

Wharton's Jelly ◽

Nerve Grafts

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Role of adipose mesenchymal stem cells and secretome in peripheral nerve regeneration

Annals of Medicine and Surgery ◽

10.1016/j.amsu.2021.102482 ◽

2021 ◽

pp. 102482

Author(s):

Tito Sumarwoto ◽

Heri Suroto ◽

Ferdiansyah Mahyudin ◽

Dwikora Novembri Utomo ◽

Romaniyanto ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Peripheral Nerve ◽

Nerve Regeneration ◽

Peripheral Nerve Regeneration ◽

Adipose Mesenchymal Stem Cells

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Rat bone marrow mesenchymal stem cells express glial markers and stimulate nerve regeneration

Neuroscience Letters ◽

10.1016/j.neulet.2004.03.077 ◽

2004 ◽

Vol 362 (3) ◽

pp. 200-203 ◽

Cited By ~ 216

Author(s):

Mel Tohill ◽

Cristina Mantovani ◽

Mikael Wiberg ◽

Giorgio Terenghi

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Nerve Regeneration ◽

Marrow Mesenchymal Stem Cells ◽

Rat Bone

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Recellularized nerve allografts with differentiated mesenchymal stem cells promote peripheral nerve regeneration

Neuroscience Letters ◽

10.1016/j.neulet.2012.02.066 ◽

2012 ◽

Vol 514 (1) ◽

pp. 96-101 ◽

Cited By ~ 64

Author(s):

Yu Wang ◽

Zhe Zhao ◽

Zhiwu Ren ◽

Bin Zhao ◽

Li Zhang ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Peripheral Nerve ◽

Nerve Regeneration ◽

Peripheral Nerve Regeneration

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The combined application of rat bone marrow mesenchymal stem cells and bioceramic materials in the regeneration of dental pulp-like tissues

10.21203/rs.2.21651/v1 ◽

2020 ◽

Author(s):

Ruixue Wen ◽

Xin Wang ◽

Yongchao Lu ◽

Yi Du ◽

Xijiao Yu

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Blood Vessels ◽

Collagen Scaffold ◽

Inflammatory Cells ◽

Combined Application ◽

Marrow Mesenchymal Stem Cells ◽

Good Biocompatibility ◽

Rat Bone

Abstract Background: To observe the effects of combined application of rat bone marrow mesenchymal stem cells (rBMSCs) and a bioceramic material on pulp-like tissue formation. Methods: Rat incisor root fragments without pulp tissues were prepared and filled with a collagen scaffold seeded with rBMSCs, while one side of the root segment was covered by a bioceramic material (iRoot BP). After culture for 12 h, the root fragments were implanted subcutaneously for 3 months. Hematoxylin and eosin (HE) staining was applied to observe biocompatibility and the formation of pulp-like tissues. Incisor root fragments were divided into three parts (BP1/3 , M1/3, and D1/3) to analyze the area and number of new vessels. Immunohistochemical staining of neuroendocrine marker PGP9.5, dentin sialophosphoprotein ( DSPP ), and vascular endothelial growth factor (VEGF) was applied to observe the formation of pulp-like tissues. Root fragments filled with the collagen scaffold only were used as a control. Results: Three months after implantation, root fragments were collected, which were surrounded by a transparent tissue membrane with a good blood supply. The root fragment cavity was filled with pink vascularized pulp-like tissue. According to HE results, iRoot BP had good biocompatibility with new pulp-like tissues and few infiltrating inflammatory cells. Increases in the number and area of new blood vessels were observed in BP1/3 compared with the other two parts. Expression of PGP9.5 and DSPP showed that the newly formed tissues were similar to normal pulp tissues. Conclusion: iRoot BP has good biocompatibility and increases the number and area of new blood vessels. The combined application of stem cells and bioceramic materials may be a better method for pulp revascularization .

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