Joint transcriptomic and metabolomic analysis reveals the mechanism of low-temperature tolerance in Hosta ventricosa

Hosta ventricosa is a robust ornamental perennial plant that can tolerate low temperatures, and which is widely used in urban landscaping design in Northeast China. However, the mechanism of cold-stress tolerance in this species is unclear. A combination of transcriptomic and metabolomic analysis was used to explore the mechanism of low-temperature tolerance in H. ventricosa. A total of 12 059 differentially expressed genes and 131 differentially expressed metabolites were obtained, which were mainly concentrated in the signal transduction and phenylpropanoid metabolic pathways. In the process of low-temperature signal transduction, possibly by transmitting Ca2+ inside and outside the cell through the ion channels on the three cell membranes of COLD, CNGCs and CRLK, H. ventricosa senses temperature changes and stimulates SCRM to combine with DREB through the MAPK signal pathway and Ca2+ signal sensors such as CBL, thus strengthening its low-temperature resistance. The pathways of phenylpropanoid and flavonoid metabolism represent the main mechanism of low-temperature tolerance in this species. The plant protects itself from low-temperature damage by increasing its content of genistein, scopolentin and scopolin. It is speculated that H. ventricosa can also adjust the content ratio of sinapyl alcohol and coniferyl alcohol and thereby alter the morphological structure of its cell walls and so increase its resistance to low temperatures.When subjected to low-temperature stress, H. ventricosa perceives temperature changes via COLD, CNGCs and CRLK, and protection from low-temperature damage is achieved by an increase in the levels of genistein, scopolentin and scopolin through the pathways of phenylpropanoid biosynthesis and flavonoid biosynthesis.

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Joint Transcriptomic and Metabolomic Analysis Reveals the Mechanism of Low Temperature Tolerance in Hosta Ventricosa

10.21203/rs.3.rs-389589/v1 ◽

2021 ◽

Author(s):

QianQian Zhuang ◽

Shaopeng Chen ◽

ZhiXin Jua ◽

Yao Yue

Keyword(s):

Signal Transduction ◽

Low Temperature ◽

Low Temperatures ◽

Temperature Tolerance ◽

Differentially Expressed ◽

Metabolomic Analysis ◽

Temperature Changes ◽

Low Temperature Tolerance ◽

Temperature Damage ◽

Hosta Ventricosa

Abstract Background: Hosta ventricosa is a robust ornamental perennial plant that can tolerate low temperatures, and which is widely used in urban landscaping design in Northeast China. However, the mechanism of cold stress tolerance in this species is unclear. Methods:This study used a combination of transcriptomic and metabolomic analysis to explore the mechanism of low temperature tolerance in H. ventricosa.Results: A total of 12 059 differentially expressed genes (DEGs) and 131 differentially expressed metabolites were obtained, which were mainly concentrated in the signal transduction and phenylpropanoid metabolic pathways. In the process of low temperature signal transduction, H. ventricosa is mainly through the ion channels on the three cell membranes of COLD, CNGCs and CRLK to transmit Ca2+ inside and outside the cell to sense temperature changes, and stimulate SCRM to combine with DREB through the MAPK signal pathway and Ca2+ signal sensors such as CBL. Strengthen the low temperature resistance of H. ventricosa. The pathways of phenylpropanoid and flavonoid metabolism represent the main mechanism of low temperature tolerance in this species. The plant protects itself from low temperature damage by increasing its content of genistein, scopolentin and scopolin. It is speculated that H. ventricosa can also adjust the content ratio of sinapyl alcohol and coniferyl alcohol and thereby alter the morphological structure of its cell walls and so increase its resistance to low temperatures.Conclusions: In H. ventricosa that is subjected to low temperature stress, temperature changes are perceived through COLD, CNGCs and CRLK, and protection from low temperature damage is achieved by an increase in the levels of genistein, scopolentin and scopolin through the pathways of phenylpropanoid biosynthesis and flavonoid biosynthesis.

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SEASONAL VARIATION IN LOW-TEMPERATURE TOLERANCE OF FRUIT-BUDS OF VACCINIUM ANGUSTIFOLIUM.

HortScience ◽

10.21273/hortsci.27.6.612d ◽

1992 ◽

Vol 27 (6) ◽

pp. 612d-612

Author(s):

Paul E. Cappiello

Keyword(s):

Seasonal Variation ◽

Low Temperature ◽

New England ◽

Vascular Tissue ◽

Temperature Tolerance ◽

Vaccinium Angustifolium ◽

Flower Primordia ◽

Factors Affecting ◽

Low Temperature Tolerance ◽

Temperature Damage

Lowbush blueberry (Vaccinium angustifolium) is a major fruit crop in costal, Northern New England and Atlantic Canada. One of the factors affecting production is low temperature damage of flower primordia. In addition to mid-winter damage, much of the damage occurs in spring due to late frosts. A study was designed to examine the seasonal variation in the LT50 of fruit buds and to determine the location of the tissue damage. Field-collected stems were exposed to controlled temperature drops and examined for damage. Three types of damage were identified; destruction of flower primordia, browning of vascular tissue within the fruit-bud, and browning of stem tissue at the base of the bud. The seasonal variation of the occurrence of this damage will be discussed.

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Identification of candidate genes for low temperature tolerance during germination of maize using a genome-wide association study and RNA-sequencing

10.21203/rs.3.rs-19588/v1 ◽

2020 ◽

Author(s):

Hong Zhang ◽

Jiayue Zhang ◽

Qingyu Xu ◽

Dandan Wang ◽

Hong Di ◽

...

Keyword(s):

Signal Transduction ◽

Low Temperature ◽

Candidate Genes ◽

Rna Sequencing ◽

Temperature Tolerance ◽

Genome Wide Association ◽

Phenotypic Traits ◽

Agricultural Crop ◽

Low Temperature Tolerance ◽

Genome Wide

Abstract Background: Maize ( Zea mays L.) is the largest agricultural crop in the world based on acreage and yield, however, it is inherently sensitive to low temperatures. The growth and yield of maize can be affected by low temperature during its whole growth period, particularly during germination. Therefore, it is urgent to identify the new gene(s) related to the low temperature tolerance during maize germination. Results: In this study, 14 phenotypic traits related to seed germination were used to explore the genetic architecture of maize through genome-wide association analysis (GWAS). A total of 30 single nucleotide polymorphisms (SNPs) associated with low temperature tolerance were detected (–log10( P ) > 4); 14 candidate genes were detected as being directly associated with these SNPs and 81 candidate genes were identified when the screen was extended to a distance of 30 kb from these SNPs. The candidate genes were predicted by conjoint analysis with RNA-sequencing (RNA-seq) to evaluate whole-genome gene expression levels. A total of nine differentially expressed genes (DEGs) (|log2foldchange|≥0.585， P <0.05) were found within distance of 30 kb, including two DEGs ( GRMZM2G101383 and GRMZM2G402584 ), which were associated with SNPs directly. The differential expression of these candidate genes was verified using qRT-PCR. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) terms of DEGs GRMZM2G052129 and GRMZM2G038964 were, ‘fatty acid metabolic process’, ‘Mitogen-activated protein kinase (MAPK) signal transduction’, and so on, which are related to the fluidity of the cell membrane and low temperature signal transduction. Conclusion: Therefore, further functional analysis of GRMZM2G052129 and GRMZM2G038964 will provide valuable information for understanding the genetic mechanism of low temperature tolerance during germination in maize.

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