scholarly journals Joint Transcriptomic and Metabolomic Analysis Reveals the Mechanism of Low Temperature Tolerance in Hosta Ventricosa

2021 ◽  
Author(s):  
QianQian Zhuang ◽  
Shaopeng Chen ◽  
ZhiXin Jua ◽  
Yao Yue
Keyword(s):  
Signal Transduction ◽  
Low Temperature ◽  
Low Temperatures ◽  
Temperature Tolerance ◽  
Differentially Expressed ◽  
Metabolomic Analysis ◽  
Temperature Changes ◽  
Low Temperature Tolerance ◽  
Temperature Damage ◽  
Hosta Ventricosa

Abstract Background: Hosta ventricosa is a robust ornamental perennial plant that can tolerate low temperatures, and which is widely used in urban landscaping design in Northeast China. However, the mechanism of cold stress tolerance in this species is unclear. Methods:This study used a combination of transcriptomic and metabolomic analysis to explore the mechanism of low temperature tolerance in H. ventricosa.Results: A total of 12 059 differentially expressed genes (DEGs) and 131 differentially expressed metabolites were obtained, which were mainly concentrated in the signal transduction and phenylpropanoid metabolic pathways. In the process of low temperature signal transduction, H. ventricosa is mainly through the ion channels on the three cell membranes of COLD, CNGCs and CRLK to transmit Ca2+ inside and outside the cell to sense temperature changes, and stimulate SCRM to combine with DREB through the MAPK signal pathway and Ca2+ signal sensors such as CBL. Strengthen the low temperature resistance of H. ventricosa. The pathways of phenylpropanoid and flavonoid metabolism represent the main mechanism of low temperature tolerance in this species. The plant protects itself from low temperature damage by increasing its content of genistein, scopolentin and scopolin. It is speculated that H. ventricosa can also adjust the content ratio of sinapyl alcohol and coniferyl alcohol and thereby alter the morphological structure of its cell walls and so increase its resistance to low temperatures.Conclusions: In H. ventricosa that is subjected to low temperature stress, temperature changes are perceived through COLD, CNGCs and CRLK, and protection from low temperature damage is achieved by an increase in the levels of genistein, scopolentin and scopolin through the pathways of phenylpropanoid biosynthesis and flavonoid biosynthesis.

scholarly journals Joint transcriptomic and metabolomic analysis reveals the mechanism of low-temperature tolerance in Hosta ventricosa

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0259455
Author(s):  
QianQian Zhuang ◽  
Shaopeng Chen ◽  
ZhiXin Jua ◽  
Yue Yao
Keyword(s):  
Signal Transduction ◽  
Low Temperature ◽  
Temperature Tolerance ◽  
Differentially Expressed ◽  
Metabolomic Analysis ◽  
Temperature Changes ◽  
Low Temperature Tolerance ◽  
Content Ratio ◽  
Temperature Damage ◽  
Hosta Ventricosa

Hosta ventricosa is a robust ornamental perennial plant that can tolerate low temperatures, and which is widely used in urban landscaping design in Northeast China. However, the mechanism of cold-stress tolerance in this species is unclear. A combination of transcriptomic and metabolomic analysis was used to explore the mechanism of low-temperature tolerance in H. ventricosa. A total of 12 059 differentially expressed genes and 131 differentially expressed metabolites were obtained, which were mainly concentrated in the signal transduction and phenylpropanoid metabolic pathways. In the process of low-temperature signal transduction, possibly by transmitting Ca2+ inside and outside the cell through the ion channels on the three cell membranes of COLD, CNGCs and CRLK, H. ventricosa senses temperature changes and stimulates SCRM to combine with DREB through the MAPK signal pathway and Ca2+ signal sensors such as CBL, thus strengthening its low-temperature resistance. The pathways of phenylpropanoid and flavonoid metabolism represent the main mechanism of low-temperature tolerance in this species. The plant protects itself from low-temperature damage by increasing its content of genistein, scopolentin and scopolin. It is speculated that H. ventricosa can also adjust the content ratio of sinapyl alcohol and coniferyl alcohol and thereby alter the morphological structure of its cell walls and so increase its resistance to low temperatures.When subjected to low-temperature stress, H. ventricosa perceives temperature changes via COLD, CNGCs and CRLK, and protection from low-temperature damage is achieved by an increase in the levels of genistein, scopolentin and scopolin through the pathways of phenylpropanoid biosynthesis and flavonoid biosynthesis.

Effects of temperature and salinity on survival of young-of-the-year Hudson River striped bass (Morone saxatilis): implications for optimal overwintering habitats

2002 ◽  
Vol 59 (5) ◽  
pp. 787-795 ◽  
Author(s):  
Thomas P Hurst ◽  
David O Conover
Keyword(s):  
Body Size ◽  
Low Temperature ◽  
Striped Bass ◽  
Low Temperatures ◽  
Morone Saxatilis ◽  
Hudson River ◽  
Temperature Tolerance ◽  
Low Temperature Tolerance ◽  
Energetic State ◽  
Young Of The Year

We examined the role of salinity, body size, and energetic state in determining low temperature tolerance of young-of-the-year (YOY) striped bass (Morone saxatilis) and used this information to map optimal overwintering habitat in the Hudson River estuary. A long-term experiment compared survival at 15 ppt and 30 ppt. In additional experiments, winter-acclimated fish were exposed to temperature declines (2.3°C·day–1 to 1°C·week–1) at salinities from 0 ppt to 35 ppt. Highest survival at low temperatures was consistently observed at intermediate salinities. These results suggest that the observed distribution of overwintering striped bass is related to physiological constraints on osmo regulatory ability at low temperatures. Low temperature tolerance appeared unrelated to body size and energetic state. Salinity profiles were used to describe the location and extent of optimal wintering habitats under various hydrographic regimes. The location of optimal habitats was displaced by over 27 km along the river axis because of variation in salinity regime. Changes in the availability of optimal habitat may be responsible for variation in recruitment to the Hudson River population. These results demonstrate the need to consider a holistic approach encompassing all seasons of the year in assessing habitat requirements of fishes.

scholarly journals Induction of Low Temperature Tolerance in Wheat by Pre-Soaking and Parental Treatment with Melatonin

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1192
Author(s):  
Hua Zhang ◽  
Lei Liu ◽  
Zongshuai Wang ◽  
Guozhong Feng ◽  
Qiang Gao ◽  
...  
Keyword(s):  
Low Temperature ◽  
Low Temperatures ◽  
Germination Rate ◽  
Grain Filling ◽  
Temperature Tolerance ◽  
Starch Degradation ◽  
Wheat Seedlings ◽  
Low Temperature Tolerance ◽  
Metabolism Enzymes ◽  
Parental Treatment

Low temperatures seriously depress germination and seedling establishment in wheat and it is of great significance to explore approaches to improve wheat tolerance to low temperatures. In this study, the effects of seed pre-soaking and parental treatment with melatonin on seed germination and low temperature tolerance during the early growing stage in wheat were studied. The results showed that pre-soaking with melatonin increased the germination rate, improved antioxidant capacity and accelerated starch degradation under low temperature, which alleviated low temperature-induced damage to the chloroplasts in coleoptiles of wheat seedlings. Parental melatonin treatment during grain filling stage significantly decreased the grain weight. Seeds from parental melatonin-treated plants showed higher germination rates and higher antioxidant enzyme activity than the control seeds under low temperature. In addition, parental treatment with melatonin modulated the activities of carbohydrate metabolism enzymes, which contributes to enhanced low temperature tolerance in wheat offspring. It was suggested that both seed pre-soaking and parental treatment with melatonin could be the effective approaches for low temperature tolerance induction in wheat.

HAPLOID SELECTION FOR LOW TEMPERATURE TOLERANCE OF TOMATO POLLEN

Genetics ◽  
1982 ◽  
Vol 101 (1) ◽  
pp. 129-137 ◽  
Author(s):  
Daniel Zamir ◽  
Steven D Tanksley ◽  
Richard A Jones
Keyword(s):  
Low Temperature ◽  
Low Temperatures ◽  
Pollen Grains ◽  
Temperature Tolerance ◽  
F1 Hybrid ◽  
Peruvian Andes ◽  
Low Temperature Tolerance ◽  
Selection For ◽  
Chromosome Segments ◽  
Haploid Selection

ABSTRACT Pollen grains were harvested from an interspecific F1 hybrid between the cultivated tomato, Lycopersicon esculentum Mill., and its wild relative Lycopersicon hirsutum Humb. & Bonpl., a low temperature tolerant accession originating from an altitude of 3200 m in the Peruvian Andes. The two species differ for electrophoretically-detectable loci that mark six (possibly seven) of the 12 tomato chromosomes. Isozyme analysis of the BC1 populations derived from controlled pollinations at normal and low temperatures indicates a significant skewing of allelic frequencies favoring two independent chromosome segments of L. hirsutum at low temperatures. The results demonstrate that gametophytic selection for low temperature tolerance of tomato pollen is determined, at least in part, by genes expressed in the haploid pollen.

scholarly journals Respiratory rates and freezing tolerance of embryos of Inga vera Willd. subsp. affinis (DC.) T.D. Penn. from different regions

2021 ◽  
Vol 43 ◽  
Author(s):  
Edmir Vicente Lamarca ◽  
Claudio José Barbedo
Keyword(s):  
Electrical Conductivity ◽  
Low Temperature ◽  
Freezing Tolerance ◽  
Low Temperatures ◽  
Temperature Tolerance ◽  
Recalcitrant Seeds ◽  
Energy Status ◽  
Low Temperature Tolerance ◽  
The Relationship ◽  
Storage Temperatures

ABSTRACT: Recalcitrant seeds are sensitive to desiccation and low storage temperatures, but there is a gradient of recalcitrance between different species and between populations of the same species. Therefore, tolerance to drying and low temperatures, as well as the respiratory rates of seeds, can be conditioned by the source of the material. The present study aimed to evaluate the relationship among desiccation and low temperature tolerance, and respiratory rates of Inga vera embryos from different regions. The embryos were submitted to three drying levels and incubated for up to fifteen days at -4, -2, 2 and 5 °C. Then, they were evaluated for respiration, germination and electrical conductivity. The embryos tolerated freezing at -4 °C since they were associated with certain levels of drying and a consequent change in the energy status of water. In conclusion, the conditions in which the embryos are formed define their degree of maturation at dispersal, hence their tolerance to desiccation and freezing.

scholarly journals SEASONAL VARIATION IN LOW-TEMPERATURE TOLERANCE OF FRUIT-BUDS OF VACCINIUM ANGUSTIFOLIUM.

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 612d-612
Author(s):  
Paul E. Cappiello
Keyword(s):  
Seasonal Variation ◽  
Low Temperature ◽  
New England ◽  
Vascular Tissue ◽  
Temperature Tolerance ◽  
Vaccinium Angustifolium ◽  
Flower Primordia ◽  
Factors Affecting ◽  
Low Temperature Tolerance ◽  
Temperature Damage

Lowbush blueberry (Vaccinium angustifolium) is a major fruit crop in costal, Northern New England and Atlantic Canada. One of the factors affecting production is low temperature damage of flower primordia. In addition to mid-winter damage, much of the damage occurs in spring due to late frosts. A study was designed to examine the seasonal variation in the LT50 of fruit buds and to determine the location of the tissue damage. Field-collected stems were exposed to controlled temperature drops and examined for damage. Three types of damage were identified; destruction of flower primordia, browning of vascular tissue within the fruit-bud, and browning of stem tissue at the base of the bud. The seasonal variation of the occurrence of this damage will be discussed.

Chilo suppressalis (striped rice stem borer).

2021 ◽  
Author(s):  
Koen Maes
Keyword(s):  
Low Temperature ◽  
East Asia ◽  
Low Temperatures ◽  
Stem Borer ◽  
Temperature Tolerance ◽  
Northern Territory ◽  
Chilo Suppressalis ◽  
Long Distance ◽  
Low Temperature Tolerance ◽  
Rice Stem Borer

Abstract This is one of the most important pests of rice in East Asia, India and Indonesia. In East Asia, C. suppressalis occurs together with Chilo hyrax and Chilo christophi. This pest was probably introduced in Spain and Hawaii by humans (Bleszynski, 1970). It is now also present in the Northern Territory of Australia. The species tolerates low to very low temperatures and is therefore present in temperate and subtropical Asia. This low temperature tolerance enables it to adapt to other regions. The fact that C. suppressalis has become established in France, Spain and Portugal indicates the potential for long-distance movements and there would seem to be a real danger that it could be introduced into Africa, or other ecologically favourable areas.

scholarly journals Identification of candidate genes for low temperature tolerance during germination of maize using a genome-wide association study and RNA-sequencing

2020 ◽  
Author(s):  
Hong Zhang ◽  
Jiayue Zhang ◽  
Qingyu Xu ◽  
Dandan Wang ◽  
Hong Di ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Low Temperature ◽  
Candidate Genes ◽  
Rna Sequencing ◽  
Temperature Tolerance ◽  
Genome Wide Association ◽  
Phenotypic Traits ◽  
Agricultural Crop ◽  
Low Temperature Tolerance ◽  
Genome Wide

Abstract Background: Maize ( Zea mays L.) is the largest agricultural crop in the world based on acreage and yield, however, it is inherently sensitive to low temperatures. The growth and yield of maize can be affected by low temperature during its whole growth period, particularly during germination. Therefore, it is urgent to identify the new gene(s) related to the low temperature tolerance during maize germination. Results: In this study, 14 phenotypic traits related to seed germination were used to explore the genetic architecture of maize through genome-wide association analysis (GWAS). A total of 30 single nucleotide polymorphisms (SNPs) associated with low temperature tolerance were detected (–log10( P ) > 4); 14 candidate genes were detected as being directly associated with these SNPs and 81 candidate genes were identified when the screen was extended to a distance of 30 kb from these SNPs. The candidate genes were predicted by conjoint analysis with RNA-sequencing (RNA-seq) to evaluate whole-genome gene expression levels. A total of nine differentially expressed genes (DEGs) (|log2foldchange|≥0.585， P <0.05) were found within distance of 30 kb, including two DEGs ( GRMZM2G101383 and GRMZM2G402584 ), which were associated with SNPs directly. The differential expression of these candidate genes was verified using qRT-PCR. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) terms of DEGs GRMZM2G052129 and GRMZM2G038964 were, ‘fatty acid metabolic process’, ‘Mitogen-activated protein kinase (MAPK) signal transduction’, and so on, which are related to the fluidity of the cell membrane and low temperature signal transduction. Conclusion: Therefore, further functional analysis of GRMZM2G052129 and GRMZM2G038964 will provide valuable information for understanding the genetic mechanism of low temperature tolerance during germination in maize.

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