scholarly journals Differential expression of miRNAs involved in biological processes responsible for inflammation and immune response in lichen sclerosus urethral stricture disease

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0261505
Author(s):  
Harjivan Kohli ◽  
Brandon Childs ◽  
Travis B. Sullivan ◽  
Artem Shevtsov ◽  
Eric Burks ◽  
...  
Keyword(s):  
Immune Response ◽  
Differential Expression ◽  
Urethral Stricture ◽  
Expression Profiles ◽  
Lichen Sclerosus ◽  
Differentially Expressed ◽  
P Value ◽  
Screening Analysis ◽  
Urethral Stricture Disease ◽  
False Discovery

Purpose To better understand the pathophysiology of lichen sclerosus (LS) urethral stricture disease (USD), we aimed to investigate expression profiles of microRNAs (miRNAs) in tissue samples from men undergoing urethroplasty. Methods Urethral stricture tissue was collected from 2005–2020. Histologic features diagnostic of LS were the basis of pathologic evaluation. Foci of areas diagnostic for LS or non-LS strictures were chosen for RNA evaluation. In an initial screening analysis, 13 LS urethral strictures and 13 non-LS strictures were profiled via miRNA RT-qPCR arrays for 752 unique miRNA. A validation analysis of 23 additional samples (9 LS and 14 non-LS) was performed for 15 miRNAs. Statistical analyses were performed using SPSS v25. Gene Ontology (GO) analysis was performed using DIANA-mirPath v. 3.0. Results In the screening analysis 143 miRNAs were detected for all samples. 27 were differentially expressed between the groups (false discovery p-value <0.01). 15 of these miRNAs individually demonstrated an area under the curve (AUC)>0.90 for distinguishing between between LS and non-LS strictures. 11-fold upregulation of MiR-155-5p specifically was found in LS vs. non-LS strictures (p<0.001, AUC = 1.0). In the validation analysis, 13 of the 15 miRNAs tested were confirmed to have differential expression (false discovery p-value <0.10). Conclusions To our knowledge this is the first study evaluating miRNA expression profiles in LS and non-LS USD. We identified several miRNAs that are differentially expressed in USD caused by LS vs other etiologies, which could potentially serve as biomarkers of LS USD. The top eight differentially expressed miRNAs have been linked to immune response processes as well as involvement in wound healing, primarily angiogenesis and fibrosis.

scholarly journals PD22-10 URINE miRNA BIOMARKERS THAT ARE DIFFERENTIALLY EXPRESSED IN LICHEN SCLEROSUS INDUCED AND NON-LICHEN SCLEROSUS INDUCED URETHRAL STRICTURE DISEASE

2021 ◽  
Vol 206 (Supplement 3) ◽  
Author(s):  
Karl Benz ◽  
Travis Sullivan ◽  
Thomas Kalantzakos ◽  
Amanda Sherman ◽  
Harjivan Kohli ◽  
...  
Keyword(s):  
Urethral Stricture ◽  
Lichen Sclerosus ◽  
Differentially Expressed ◽  
Urethral Stricture Disease

scholarly journals Synchronous genitourinary lichen sclerosus signals a distinct urinary microbiome profile in men with urethral stricture disease

2020 ◽  
Author(s):  
Andrew J. Cohen ◽  
Thomas W. Gaither ◽  
Sudarshan Srirangapatanam ◽  
Erick R. Castellanos ◽  
Anthony Enriquez ◽  
...  
Keyword(s):  
Urethral Stricture ◽  
Lichen Sclerosus ◽  
Urethral Stricture Disease ◽  
Microbiome Profile ◽  
Urinary Microbiome

scholarly journals Global Long Noncoding RNA and mRNA Expression Changes between Prenatal and Neonatal Lung Tissue in Pigs

Genes ◽  
2018 ◽  
Vol 9 (9) ◽  
pp. 443 ◽  
Author(s):  
Long Jin ◽  
Silu Hu ◽  
Teng Tu ◽  
Zhiqing Huang ◽  
Qianzi Tang ◽  
...  
Keyword(s):  
Immune Response ◽  
Cell Proliferation ◽  
Lung Tissue ◽  
Long Noncoding Rna ◽  
Lung Development ◽  
Noncoding Rna ◽  
Differentially Expressed ◽  
P Value ◽  
Distal Lung ◽  
Neonatal Lungs

Lung tissue plays an important role in the respiratory system of mammals after birth. Early lung development includes six key stages, of which the saccular stage spans the pre- and neonatal periods and prepares the distal lung for alveolarization and gas-exchange. However, little is known about the changes in gene expression between fetal and neonatal lungs. In this study, we performed transcriptomic analysis of messenger RNA (mRNA) and long noncoding RNA (lncRNA) expressed in the lung tissue of fetal and neonatal piglets. A total of 19,310 lncRNAs and 14,579 mRNAs were identified and substantially expressed. Furthermore, 3248 mRNAs were significantly (FDR-adjusted p value ≤ 0.05, FDR: False Discovery Rate) differentially expressed and were mainly enriched in categories related to cell proliferation, immune response, hypoxia response, and mitochondrial activation. For example, CCNA2, an important gene involved in the cell cycle and DNA replication, was upregulated in neonatal lungs. We also identified 452 significantly (FDR-adjusted p value ≤ 0.05) differentially expressed lncRNAs, which might function in cell proliferation, mitochondrial activation, and immune response, similar to the differentially expressed mRNAs. These results suggest that differentially expressed mRNAs and lncRNAs might co-regulate lung development in early postnatal pigs. Notably, the TU64359 lncRNA might promote distal lung development by up-regulating the heparin-binding epidermal growth factor-like (HB-EGF) expression. Our research provides basic lung development datasets and will accelerate clinical researches of newborn lung diseases with pig models.

Analysis of the miRNA transcriptome during testicular development and spermatogenesis of the Mongolian horse

2020 ◽  
Vol 32 (6) ◽  
pp. 582
Author(s):  
Bei Li ◽  
Xiaolong He ◽  
Yiping Zhao ◽  
Dongyi Bai ◽  
Dandan Li ◽  
...  
Keyword(s):  
Target Genes ◽  
Expression Profiles ◽  
Mammalian Species ◽  
Mature Mirnas ◽  
Differentially Expressed ◽  
Small Rna Sequencing ◽  
P Value ◽  
Testicular Development ◽  
Interaction Sites ◽  
Mongolian Horse

Numerous studies have shown that microRNAs (miRNAs) are essential for testicular development and spermatogenesis. In order to further characterise these physiological processes, three immature and three mature testes of the Mongolian horse were collected and six libraries were established. Using small RNA sequencing technology, 531 mature miRNAs were identified, including 46 novel miRNAs without previously ascribed functions. Among the 531 miRNAs, 421 were expressed in both immature and mature libraries, 65 miRNAs were found solely in immature testis libraries and 45 miRNAs were found solely in mature testis libraries. Furthermore, among the miRNAs that were identified in both immature and mature libraries, 107 were significantly differentially expressed (corrected P value (padj)&lt;0.05). Among the miRNAs that were only expressed in immature testes, two miRNAs were differentially expressed, whereas among the miRNAs that were only expressed in mature testes, nine miRNAs were differentially expressed. Comprehensive analysis of miRNA and mRNA expression profiles predicted 107 miRNA–mRNA interaction sites. Gene ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis of the predicted target genes suggested roles of the differentially expressed miRNAs in testicular development and spermatogenesis. These findings identify miRNAs as key factors in the development of the testes and spermatogenesis in the Mongolian horse, which may also help us to understand the mechanisms of fertility in related mammalian species.

MP60-19 EXAMINING THE PATHOPHYSIOLOGY OF URETHRAL STRICTURE DISEASE DUE TO LICHEN SCLEROSUS

2020 ◽  
Vol 203 ◽  
pp. e898
Author(s):  
Harjivan Kohli* ◽  
Brandon Childs ◽  
Travis Sullivan ◽  
Thomas Kalantzakos ◽  
Eric Burks ◽  
...  
Keyword(s):  
Urethral Stricture ◽  
Lichen Sclerosus ◽  
Urethral Stricture Disease

Lichen Sclerosus and Isolated Bulbar Urethral Stricture Disease

2014 ◽  
Vol 192 (3) ◽  
pp. 775-779 ◽  
Author(s):  
Joceline S. Liu ◽  
Kelly Walker ◽  
Daniel Stein ◽  
Sanjiv Prabhu ◽  
Matthias D. Hofer ◽  
...  
Keyword(s):  
Urethral Stricture ◽  
Lichen Sclerosus ◽  
Urethral Stricture Disease

scholarly journals Fecal Host Transcriptomics for Non-invasive Human Mucosal Immune Profiling: Proof of Concept in Clostridium difficile Infection

2018 ◽  
Vol 3 (2) ◽  
pp. 164
Author(s):  
Robert Schlaberg ◽  
Amanda Barrett ◽  
Kornelia Edes ◽  
Michael Graves ◽  
Litty Paul ◽  
...  
Keyword(s):  
Immune Response ◽  
Clostridium Difficile ◽  
Differentially Expressed Genes ◽  
Clostridium Difficile Infection ◽  
Expression Profiles ◽  
Principal Component ◽  
Mucosal Immune Response ◽  
Differentially Expressed ◽  
Proof Of Concept ◽  
Non Invasive

Background: Host factors play an important role in pathogenesis and disease outcome in Clostridium difficile infection (CDI), and characterization of these responses could uncover potential host biomarkers to complement existing microbe-based diagnostics.Methods: We extracted RNA from fecal samples of patients with CDI and profiled human mRNA using amplicon-based next-generation sequencing (NGS). We compared the fecal host mRNA transcript expression profiles of patients with CDI to controls with non-CDI diarrhea.Results: We found that the ratio of human actin gamma 1 (ACTG1) to 16S ribosomal RNA (rRNA) was highly correlated with NGS quality as measured by percentage of reads on target. Patients with CDI could be differentiated from those with non-CDI diarrhea based on their fecal mRNA expression profiles using principal component analysis. Among the most differentially expressed genes were ones related to immune response (IL23A, IL34) and actin-cytoskeleton function (TNNT1, MYL4, SMTN, MYBPC3, all adjusted P-values <1×10-3).Conclusions: In this proof-of-concept study, we used host fecal transcriptomics for non-invasive profiling of the mucosal immune response in CDI. We identified differentially expressed genes with biological plausibility based on animal and cell culture models. This demonstrates the potential of fecal transcriptomics to uncover host-based biomarkers for enteric infections.

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