scholarly journals The Effect of Pretreatments on Surfactin Production from Potato Process Effluent by Bacillus subtilis

2001 ◽  
Vol 91-93 (1-9) ◽  
pp. 487-502 ◽  
Author(s):  
David N. Thompson ◽  
Sandra L. Fox ◽  
Gregory A. Bala
Author(s):  
Karl S. Noah ◽  
Sandra L. Fox ◽  
Debby F. Bruhn ◽  
David N. Thompson ◽  
Gregory A. Bala

2014 ◽  
Vol 70 (2) ◽  
pp. 234-240 ◽  
Author(s):  
G. A. Amin

Surfactin produced by Bacillus subtilis BDCC-TUSA-3 from Maldex-15 was used as a growth-associated product in a conventional batch process. Maldex-15 is a cheap industrial by-product recovered during manufacturing of high fructose syrup from corn starch. Surfactin production was greatly improved in exponential fed-batch fermentation. Maldex-15 and other nutrients were exponentially fed into the culture based on the specific growth rate of the bacterium. In order to maximize surfactin yield and productivity, conversion of different quantities of Maldex-15 into surfactin was investigated in five different fermentation runs. In all runs, most of the Maldex-15 was consumed and converted into surfactin and cell biomass with appreciable efficiencies. The best results were obtained with the fermentation run supplied with 204 g Maldex-15. Up to 36.1 g l−1 of surfactin and cell biomass of 31.8 g l−1 were achieved in 12 h. Also, a marked substrate yield of 0.272 g g−1 and volumetric reactor productivity of 2.58 g 1−1 h−1 were obtained, confirming the establishment of a cost-effective commercial surfactin production.


Author(s):  
Patrícia Maria Rocha ◽  
Ana Carmen dos Santos Mendes ◽  
Sérgio Dantas de Oliveira Júnior ◽  
Carlos Eduardo de Araújo Padilha ◽  
Ana Laura Oliveira de Sá Leitão ◽  
...  

2014 ◽  
Vol 21 (9) ◽  
pp. 885-893 ◽  
Author(s):  
Namir Haddad ◽  
Hongzong Gang ◽  
Jinfeng Liu ◽  
Serge Mbadinga ◽  
Bozhong Mu

2014 ◽  
Vol 81 (1) ◽  
pp. 203-210 ◽  
Author(s):  
Susanne Müller ◽  
Sarah N. Strack ◽  
Sarah E. Ryan ◽  
Daniel B. Kearns ◽  
John R. Kirby

ABSTRACTBiofilm formation is a common mechanism for surviving environmental stress and can be triggered by both intraspecies and interspecies interactions. Prolonged predator-prey interactions between the soil bacteriumMyxococcus xanthusandBacillus subtiliswere found to induce the formation of a new type ofB. subtilisbiofilm, termed megastructures. Megastructures are tree-like brachiations that are as large as 500 μm in diameter, are raised above the surface between 150 and 200 μm, and are filled with viable endospores embedded within a dense matrix. Megastructure formation did not depend on TasA, EpsE, SinI, RemA, or surfactin production and thus is genetically distinguishable from colony biofilm formation on MSgg medium. AsB. subtilisendospores are not susceptible to predation byM. xanthus, megastructures appear to provide an alternative mechanism for survival. In addition,M. xanthusfruiting bodies were found immediately adjacent to the megastructures in nearly all instances, suggesting thatM. xanthusis unable to acquire sufficient nutrients from cells housed within the megastructures. Lastly, aB. subtilismutant lacking the ability to defend itself via bacillaene production formed megastructures more rapidly than the parent. Together, the results indicate that production of the megastructure facilitatesB. subtilisescape into dormancy via sporulation.


2009 ◽  
Vol 155 (1-3) ◽  
pp. 63-75 ◽  
Author(s):  
Maria Valderez Ponte Rocha ◽  
Raphaela V. Gomes Barreto ◽  
Vânia Maria M. Melo ◽  
Luciana Rocha Barros Gonçalves

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Dhouha Ghribi ◽  
Semia Ellouze-Chaabouni

Interest in biosurfactants has increased considerably in recent years, as they are potentially used in many commercial applications in petroleum, pharmaceuticals, biomedical, and food processing industries. Since improvement of their production was of great importance to reduce the final coast, cultural conditions were analyzed to optimize biosurfactants production from Bacillus subtilis SPB1 strain. A high yield of biosurfactants was obtained from a culture of B. subtilis using carbohydrate substrate as a carbon source; among carbohydrates, glucose enhanced the best surfactin production. The optimum glucose concentration was 40 g/L. Higher amount of biosurfactants was obtained using 5 g/L of urea as organic nitrogen source and applying C/N ratio of 7 with ammonium chloride as inorganic nitrogen source. The highest amount of biosurfactants was recorded with the addition of 2% kerosene. Moreover, it was shown, using an automated full-controlled 2.6 L fermenter, that aeration of the medium, which affected strongly the growth regulated biosurfactants synthesis by the producing cell. So that, low or high aerations lead to a decrease of biosurfactants synthesis yields. It was found that when using dissolved oxygen saturation of the medium at 30%, biosurfactants production reached 4.92 g/L.


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