Exponential fed-batch strategy for enhancing biosurfactant production by Bacillus subtilis

2014 ◽  
Vol 70 (2) ◽  
pp. 234-240 ◽  
Author(s):  
G. A. Amin
Keyword(s):  
Bacillus Subtilis ◽  
Corn Starch ◽  
Cost Effective ◽  
Batch Process ◽  
Fed Batch ◽  
Cell Biomass ◽  
High Fructose ◽  
Fed Batch Fermentation ◽  
High Fructose Syrup ◽  
Surfactin Production

Surfactin produced by Bacillus subtilis BDCC-TUSA-3 from Maldex-15 was used as a growth-associated product in a conventional batch process. Maldex-15 is a cheap industrial by-product recovered during manufacturing of high fructose syrup from corn starch. Surfactin production was greatly improved in exponential fed-batch fermentation. Maldex-15 and other nutrients were exponentially fed into the culture based on the specific growth rate of the bacterium. In order to maximize surfactin yield and productivity, conversion of different quantities of Maldex-15 into surfactin was investigated in five different fermentation runs. In all runs, most of the Maldex-15 was consumed and converted into surfactin and cell biomass with appreciable efficiencies. The best results were obtained with the fermentation run supplied with 204 g Maldex-15. Up to 36.1 g l−1 of surfactin and cell biomass of 31.8 g l−1 were achieved in 12 h. Also, a marked substrate yield of 0.272 g g−1 and volumetric reactor productivity of 2.58 g 1−1 h−1 were obtained, confirming the establishment of a cost-effective commercial surfactin production.

scholarly journals Bacillus subtilis High Cell Density Fermentation Using a Sporulation-Deficient Strain for the Production of Surfactin

2021 ◽  
Author(s):  
Peter Klausmann ◽  
Katja Hennemann ◽  
Mareen Hoffmann ◽  
Chantal Treinen ◽  
Moritz Aschern ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Cell Density ◽  
High Cell Density ◽  
High Titer ◽  
Batch Process ◽  
High Cell ◽  
Fed Batch ◽  
Fourfold Increase ◽  
High Cell Density Fermentation ◽  
Surfactin Production

Abstract Bacillus subtilis 3NA is a strain capable of reaching high cell densities. A surfactin producing sfp+ variant of this strain, named JABs32, was utilized in fed-batch cultivation processes. Both a glucose and an ammonia solution were fed to set a steady growth rate μ of 0.1 h-1. In this process, a cell dry weight of up to 88 g L-1 was reached after 38 h of cultivation, and surfactin titers of up to 26.5 g L-1 were detected in this high cell density fermentation process, achieving a YP/X value of 0.23 g g-1 as well as a qP/X of 0.007 g g-1 h-1. In sum, a 21-fold increase in surfactin titer was obtained compared with cultivations in shake flasks. In contrast to fed-batch operations using Bacillus subtilis JABs24, an sfp+ variant derived from B. subtilis 168, JABs32, reached an up to fourfold increase in surfactin titers using the same fed-batch protocol. Additionally, a two-stage feed process was established utilizing strain JABs32. Using an optimized mineral salt medium in this high cell density fermentation approach, after 31 h of cultivation, surfactin titers of 23.7 g L-1 were reached with a biomass concentration of 41.3 g L-1, thus achieving an enhanced YP/X value of 0.57 g g-1 as well as a qP/X of 0.018 g g-1 h-1. The mutation of spo0A locus and an elongation of AbrB in the strain utilized in combination with a high cell density fed-batch process represents a promising new route for future enhancements on surfactin production. Key points • Utilization of a sporulation deficient strain for fed-batch operations • High cell density process with Bacillus subtilis for lipopeptide production was established • High titer surfactin production capabilities confirm highly promising future platform strain

scholarly journals Engineering Bacillus Subtilis for the production of High Fructose Syrup: Opportunities and Prospects

2021 ◽  
Vol 6 (2) ◽  
pp. 101-109
Author(s):  
Ildephonse Habimana ◽  
Qiao Zhina ◽  
Aqeel Sahibzada Muhammad ◽  
Jean Damascene Harindintwali ◽  
Abdulqader Al-Adeeb ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
High Fructose ◽  
High Fructose Syrup

scholarly journals Optimization of cyclosporin A production by Beauveria nivea in continuous fed-batch fermentation

2011 ◽  
Vol 63 (3) ◽  
pp. 907-914 ◽  
Author(s):  
Huijun Dong ◽  
Junyun Jiang ◽  
Tongshun Yan ◽  
Junjie Zhao
Keyword(s):  
Cyclosporin A ◽  
Batch Fermentation ◽  
Feeding Rate ◽  
Control Method ◽  
Batch Process ◽  
Effective Control ◽  
Fed Batch ◽  
Residual Concentration ◽  
Fed Batch Fermentation ◽  
Continuous Feeding

To develop the effective control method for fed-batch culture of cyclosporin A production, we chose fructose, L-valine and (NH4)2HPO4 as feeding nutrients and compared their productivities in relation to different concentrations. The feeding rate of three kinds of feeding materials was controlled to maintain the suitable residual concentration. The fed-batch fermentation results indicated that the optimal concentrations of fructose, L-valine and (NH4)2HPO4 were about 20 g/L, 0.5 g/L and 0.6 g/L for cyclosporin A production, respectively. The cultivation of Beauveria nivea could produce cyclosporin A up to 6.2 g/L for 240 hrs through a continuous feeding-rate-controlled-batch process under the optimal feeding conditions.

scholarly journals Optimized recombinant Bacillus Subtilis 168 whole-cell catalyzes one-step biosynthesis of high fructose syrup

2021 ◽  
Vol 6 (2) ◽  
pp. 067-075
Author(s):  
Ildephonse Habimana ◽  
Qiao Zhina ◽  
Aqeel Sahibzada Muhammad ◽  
Jean Damascene Harindintwali ◽  
Al-Adeeb Abdulquader ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Whole Cell ◽  
Bacillus Subtilis 168 ◽  
High Fructose ◽  
One Step ◽  
High Fructose Syrup ◽  
Recombinant Bacillus Subtilis

scholarly journals Enhancing Production of Alkaline Polygalacturonate Lyase from Bacillus subtilis by Fed-Batch Fermentation

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e90392 ◽  
Author(s):  
Mouyong Zou ◽  
Fenfen Guo ◽  
Xuezhi Li ◽  
Jian Zhao ◽  
Yinbo Qu
Keyword(s):  
Bacillus Subtilis ◽  
Batch Fermentation ◽  
Fed Batch ◽  
Fed Batch Fermentation ◽  
Polygalacturonate Lyase ◽  
Enhancing Production

scholarly journals Influence of trace elements supplementation on the production of recombinant frutalin by Pichia pastoris KM71H in fed-batch process

2013 ◽  
Vol 67 (7) ◽  
Author(s):  
Marcela Wanderley ◽  
Carla Oliveira ◽  
Danyelly Bruneska ◽  
Lucília Domingues ◽  
José Lima Filho ◽  
...  
Keyword(s):  
Trace Elements ◽  
Pichia Pastoris ◽  
Recombinant Protein ◽  
Protein Production ◽  
Batch Process ◽  
Fermentation Medium ◽  
Fed Batch ◽  
Stirred Tank Bioreactor ◽  
Recombinant Pichia Pastoris ◽  
Fed Batch Fermentation

AbstractFrutalin, a galactose-specific lectin used to detect specific tumour markers, is a protein with low expression level in breadfruit. In the present study, fed-batch fermentation in a stirred tank bioreactor was used as a strategy to enhance protein production by a recombinant Pichia pastoris KM71H. By using this process, the production of recombinant frutalin was 4-fold higher than the value obtained in shaker flasks batch assays. Supplementation of the fermentation medium with trace elements (Pichia trace minerals, PTM) was also evaluated in order to stimulate production of the recombinant protein. The addition of PTM to the minimum medium afforded a recombinant protein production of 13.4 mg L−1, which was 2.5-fold higher than that achieved from the culture medium without PTM supplementation. These results are significant as the development of strategies to improve the production of recombinant frutalin may broaden its application in cancer diagnosis.

scholarly journals PRODUCTION OF DOCOSAHEXAENOIC ACID (DHA) FROM Thraustochytrium sp. ATCC 26185 USING DIFFERENTS NITROGEN CONCENTRATIONS

2014 ◽  
Vol 32 (1) ◽  
Author(s):  
VALCENIR JUNIOR MENDES FURLAN ◽  
MARIA DO CASTELO PAULO ◽  
VICTOR WEGNER MAUS ◽  
JULIA FERREIRA ◽  
IRINEU BATISTA ◽  
...  
Keyword(s):  
Cell Concentration ◽  
Batch Process ◽  
Fed Batch ◽  
The Third ◽  
Culture Time ◽  
Cell Biomass ◽  
Oleaginous Microorganism ◽  
Nitrogen Concentrations ◽  
Maximum Cell ◽  
Maximum Cell Concentration

In this work it was studied the polyunsaturated fatty acids (PUFAs) production, especially DHA, from Thraustochytrium sp. ATCC 26185, under different total nitrogen (TN) availability. Three different TN conditions were evaluated: two with initial concentrations of 2.4 g/L and 0.8 g/L, and the third in a fed-batch process with a rate of 0.009 g/L.h. For each experiment the biomass, glucose, TN and PUFAs were determined. The major composition of the PUFAs in Thraustochytrium sp. ATCC 26185 cell biomass were DPA ω6 (21-25 %) and DHA (69-73 %), regardless of the type and time of culture. The maximum cell concentration (30.2 g/L) was obtained using 2.4 g/L TN in 168 h of culture. With this same concentration of TN it was possible to produce the highest concentration of DHA (1.16 g/L) in 120 h of culture, demonstrating that the growth of Thraustochytrium sp. ATCC 26185 and yield of PUFAs are dependent on the source concentration of TN available for consumption of this oleaginous microorganism, as well as culture time.

scholarly journals High Cell Density Cultivation of A Recombinant Bacillus Subtilis for Nattokinase Production

2020 ◽  
Author(s):  
Jianhua Zhang ◽  
Qing Cui ◽  
Bingjun Qian ◽  
Xiangjun Sun
Keyword(s):  
Enzyme Activity ◽  
Bacillus Subtilis ◽  
Cell Density ◽  
Large Scale ◽  
High Cell Density ◽  
Specific Activity ◽  
Feeding Strategy ◽  
Batch Process ◽  
High Cell ◽  
Fed Batch

Abstract Background: Nattokinase (NK), a fibrinolytic enzyme, can be produced by culturing recombinant Bacillus subtilis in Luria-Bertani broth in a shaking flask. For use as a nutraceutical, however, a large-scale preparation and a simple purification process are required.Results: The present study utilized a fed-batch process to cultivate a B. subtilis strain carrying a pHT01 plasmid with an NK-encoding gene (B. subtilis/pHT01-aprN1). For batch A (FB A), with a pH-stat two-stage fermentation strategy, we achieved an activity of 2910.5 ± 21.6 U mL-1 and a specific activity of 30.32 U ml-1 OD600-1. Then, we changed the strategy with a later induction and lower feeding rate to pursue higher cell density and thus higher enzyme activity, a 11.9-fold activity of 4521.8 ± 23.8 U mL-1 was acquired, however, the specific activity was lower than FB A. For the third batch, low-glycerol-level-maintain feeding strategy was followed, and finally, a NK activity of 7778 ±17.28 U mL-1 was obtained, according to our knowledge, it was the highest activity assayed by the fibrin plate method ever reported. Furthermore, fermentation supernatant was successively purified by ammonium sulfate precipitation and nickel column affinity chromatography with a total NK recovery rate of 65.2%.Conclusions: Our results indicate that there is a balance between the cell growth rate and NK expression when recombinant Bacillus subtilis is cultured with a fed-batch process. The equilibrium state can be attained by optimizing the induction and feeding strategy, and thus a high cell density and enzyme activity can be achieved.

Sign in / Sign up

Export Citation Format

Share Document