Inhibitory Effects of Echinoisoflavanone and Sophoraisoflavanone D in Sophora chrysophylla SEEM on Lipid Peroxidation of Mice Brain Homogenate by Interaction of Ferrous Ion and Hydrogen Peroxide, In Vitro

2001 ◽  
Vol 80 (2) ◽  
pp. 139-144 ◽  
Author(s):  
Shizuo Toda ◽  
Yoshiaki Shirataki
Keyword(s):  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Brain Homogenate ◽  
Ferrous Ion ◽  
Inhibitory Effects ◽  
Mice Brain

Phenolic constituents and inhibitory effects of the leaf of Rauvolfia vomitoria Afzel on free radicals, cholinergic and monoaminergic enzymes in rat’s brain in vitro

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Olubukola H. Oyeniran ◽  
Adedayo O. Ademiluyi ◽  
Ganiyu Oboh
Keyword(s):  
Lipid Peroxidation ◽  
Free Radicals ◽  
Methanolic Extract ◽  
Array Detector ◽  
Oh Radicals ◽  
Inhibitory Effects ◽  
Methanolic Extracts ◽  
Phenolic Constituents ◽  
Rauvolfia Vomitoria

AbstractObjectivesRauvolfia vomitoria is a medicinal plant used traditionally in Africa in the management of several human diseases including psychosis. However, there is inadequate scientific information on the potency of the phenolic constituents of R. vomitoria leaf in the management of neurodegeneration. Therefore, this study characterized the phenolic constituents and investigated the effects of aqueous and methanolic extracts of R. vomitoria leaf on free radicals, Fe2+-induced lipid peroxidation, and critical enzymes linked to neurodegeneration in rat’s brain in vitro.MethodsThe polyphenols were evaluated by characterizing phenolic constituents using high-performance liquid chromatography coupled with diode array detector (HPLC-DAD). The antioxidant properties were assessed through the extracts ability to reduce Fe3+ to Fe2+; inhibit ABTS, DPPH, and OH radicals and Fe2+-induced lipid peroxidation. The effects of the extracts on AChE and MAO were also evaluated.ResultsThe phenolic characterization of R. vomitoria leaf revealed that there were more flavonoids present. Both aqueous and methanolic extracts of R. vomitoria leaf had inhibitory effects with the methanolic extract having higher significant (p≤0.05) free radicals scavenging ability coupled with inhibition of monoamine oxidases. However, there was no significant (p≤0.05) difference obtained in the inhibition of lipid peroxidation and cholinesterases.ConclusionThis study suggests that the rich phenolic constituents of R. vomitoria leaf might contribute to the observed antioxidative and neuroprotective effects. The methanolic extract was more potent than the aqueous extract; therefore, extraction of R. vomitoria leaf with methanol could offer better health-promoting effects in neurodegenerative condition.

scholarly journals Suppression of lipid peroxidation in adrenal microsomes following ACTH administration to guinea pigs

2001 ◽  
Vol 168 (2) ◽  
pp. 333-338 ◽  
Author(s):  
JM Burczynski ◽  
JM Voigt ◽  
PA Longhurst ◽  
HD Colby ◽  
Keyword(s):  
Lipid Peroxidation ◽  
Guinea Pigs ◽  
Beta Carotene ◽  
Alpha Tocopherol ◽  
Inhibitory Effects ◽  
Hormone Production ◽  
Adrenal Hormone ◽  
Acth Treatment ◽  
Dose Dependent

Previous studies demonstrated high levels of lipid peroxidation (LP) in the guinea pig adrenal cortex. The present studies were done to determine if adrenal LP activity was influenced by ACTH, the major hormonal regulator of the gland. Guinea pigs were treated with ACTH for 1, 3 or 7 days. In addition, some guinea pigs received ACTH for 7 days and were killed 3 or 7 days later. After treatment, adrenal microsomal fractions were prepared and incubated in vitro with 1 mM ferrous sulfate to initiate LP. ACTH treatment caused a progressive decrease in adrenal LP; activity was almost totally inhibited within 3 days. The inhibitory effects of ACTH on LP were dose-dependent. Following cessation of ACTH treatment, adrenal LP gradually returned toward control levels. Microsomal concentrations of linoleic acid, a major substrate for adrenal LP, were increased by ACTH administration and then also returned to control levels after cessation of treatment. There were no significant changes in adrenal alpha-tocopherol or beta-carotene concentrations resulting from ACTH treatment. The results indicate that ACTH has a role in the regulation of adrenal LP. The actions of ACTH cannot be attributed to an increase in adrenal content of the antioxidants, alpha-tocopherol and beta-carotene, or to a decrease in LP substrate. The actions of ACTH to inhibit LP may contribute to an increase in adrenal hormone production by protecting steroidogenic enzymes from peroxidative degradation.

scholarly journals In-vitro Anti-lipid Peroxidation and Other Antioxidant Potentials of Gongronema latifolium Leaf Extract

2021 ◽  
pp. 35-41
Author(s):  
Tebekeme Okoko ◽  
Daniel I. Silas-Olu
Keyword(s):  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Plant Extract ◽  
Leaf Extract ◽  
Liver Homogenate ◽  
Bovine Liver ◽  
Herbaceous Plant ◽  
Reducing Ability ◽  
Gongronema Latifolium

Gongronema latifolium is a herbaceous plant consumed as a traditional folk medicine. The aim of this work is to evaluate the anti-lipid peroxidation activity and other antioxidant effects of the plant leaf extract of Gongronema latifolium using various in vitro models. Lipid peroxidation was first induced in egg yolk and bovine liver homogenate using the ascorbate-ferrous system and incubated with the plant extract at different concentrations. In another experiment, hydrogen peroxide was used to induce erythrocyte hemolysis and lipid peroxidation and those erythrocytes were incubated with the extract. Finally the potential ferrous reducing ability, hydroxyl radical and hydrogen peroxide scavenging activities of the plant extract were also analyzed. It revealed that the leaf extract significantly reduced chemically-induced lipid peroxidation in both homogenates when compared to quercetin. The extract also reduced both hydrogen peroxide-induced hemolysis and lipid peroxidation in erythrocytes. The extract also possessed significant abilities to reduce ferric ions, scavenge both hydroxyl radicals and hydrogen peroxide. In most cases, the responses were concentration-dependent (p < 0.05). The findings are ascribed to the important phytochemicals which are antioxidant in nature hence the plant could be exploited both pharmacologically and neutraceutically.

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