scholarly journals In-vitro Anti-lipid Peroxidation and Other Antioxidant Potentials of Gongronema latifolium Leaf Extract

2021 ◽  
pp. 35-41
Author(s):  
Tebekeme Okoko ◽  
Daniel I. Silas-Olu
Keyword(s):  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Plant Extract ◽  
Leaf Extract ◽  
Liver Homogenate ◽  
Bovine Liver ◽  
Herbaceous Plant ◽  
Reducing Ability ◽  
Gongronema Latifolium

Gongronema latifolium is a herbaceous plant consumed as a traditional folk medicine. The aim of this work is to evaluate the anti-lipid peroxidation activity and other antioxidant effects of the plant leaf extract of Gongronema latifolium using various in vitro models. Lipid peroxidation was first induced in egg yolk and bovine liver homogenate using the ascorbate-ferrous system and incubated with the plant extract at different concentrations. In another experiment, hydrogen peroxide was used to induce erythrocyte hemolysis and lipid peroxidation and those erythrocytes were incubated with the extract. Finally the potential ferrous reducing ability, hydroxyl radical and hydrogen peroxide scavenging activities of the plant extract were also analyzed. It revealed that the leaf extract significantly reduced chemically-induced lipid peroxidation in both homogenates when compared to quercetin. The extract also reduced both hydrogen peroxide-induced hemolysis and lipid peroxidation in erythrocytes. The extract also possessed significant abilities to reduce ferric ions, scavenge both hydroxyl radicals and hydrogen peroxide. In most cases, the responses were concentration-dependent (p < 0.05). The findings are ascribed to the important phytochemicals which are antioxidant in nature hence the plant could be exploited both pharmacologically and neutraceutically.

scholarly journals Assessment of the Inherent in-vitro Antioxidant Potential of Commelina benghalensis Leaf Extract

2021 ◽  
pp. 25-32
Author(s):  
Tebekeme Okoko
Keyword(s):  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Hydroxyl Radical ◽  
Leaf Extract ◽  
Bovine Liver ◽  
Egg Yolk ◽  
Commelina Benghalensis ◽  
Ferrous Ascorbate

Commelina benghalensis is a troublesome but exotic weed native to the African and Asian subregions used traditionally for the treatment and management of various disorders. The aim of this study was to investigate the potential antioxidant activity of the methanolic leaf extract of Commelina benghalensis using various in vitro models. This was done by investigating the ability of the extract to scavenge hydrogen peroxide and hydroxyl radical. Other activities assessed were the reducing ability, ability to inhibit erythrocyte damage and reduce ferrous-ascorbate induced lipid peroxidation on bovine liver and egg yolk homogenates. The results revealed that the plant extract possessed significant hydrogen peroxide and hydroxyl radical scavenging abilities. The extract also possessed  significant ability to reduce ferric ions and molybdate VI. The methanolic extract also significantly inhibited hydrogen peroxide-induced erythrocyte hemolysis and lipid peroxidation. Lipid peroxidation in bovine liver and egg yolk homogenates induced       by the ferrous-ascorbate system was also reduced by the extract. In many instances, the effect of the extract was concentration-dependent. (p < 0.05). This antioxidant activity of the extract is ascribed to the phytochemicals which probably acted in synergy thus the Commelina benghalensis leaves could be exploited both nutraceutically and pharmacologically.

scholarly journals In Vitro antioxidant activity of Polygonum Glabrum 

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Raja Sundara rajan ◽  
Ramya I
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Plant Extract ◽  
Superoxide Anion ◽  
Methanol Extract ◽  
Butylated Hydroxytoluene ◽  
In Vitro Antioxidant Activity

<p>Assessment of antioxidant activity was imperative in the screening of medicinal plants for potential health benefits. In present study methanol extract of <em>Polygonum glabrum</em> <em> </em>(polygonaceae) was screened for its in vitro antioxidant activity using biologically relevant methodologies which scavenge radicals such as  1,1 diphenyl 2 picryl hydrazyl (DPPH), nitric oxide, hydrogen peroxide, hydroxyl,  superoxide anion and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS). Total reducing ability by conversion of ferric (III) to ferrous (II) and molybdenum (VI) to molybdenum (V), metal ion chelating capacity and anti lipid peroxidation activities were also examined. The antioxidant ability of <em>Polygonum glabrum</em> whole plant extract was found to be in a dose dependent manner. The IC<sub>50</sub> values for scavenging of DPPH<sup>●</sup> and ABTS<sup>●+</sup> free radicals were 13.18 μg/ml and 20.46 μg/ml. For scavenging of nitric oxide, hydrogen peroxide, hydroxyl and superoxide anion radicals, the IC<sub>50</sub> values were found to be 80.22 μg/ml, 33.06 μg/ml, 52.26μg/ml and 36.98 μg/ml respectively. Further, addition of 120μg/ml of plant extract to the reaction mixture produced 50% lipid peroxidation inhibition activity. Commercial antioxidants such as vitamin E, quercetin, butylated hydroxytoluene and ascorbic acid were used as reference compounds. The strong antioxidant activity of <em>Polygonum glabrum</em> may be credited to the presence of triterpenes [beta-hydroxyfriedalanol], phenols        [3-hydroxy-5-methoxystilbene], flavonoids [pinocembrin and pinocembrin-5-methylether], steroids [sitosterol - (6-O-palmitoyl)- 3-O-β-D glucopyranoside and sitosterol-3-O-β-D glucopyranoside], sesqueterpenes [2,3-dihydroxy isodrimeninol] and pigments etc in methanol extract.</p>

Bitter Melon Protects Against Lipid Peroxidation Caused by Immobilization Stress in Albino Rats

2009 ◽  
Vol 79 (1) ◽  
pp. 48-56 ◽  
Author(s):  
Chaturvedi
Keyword(s):  
Lipid Peroxidation ◽  
Immobilization Stress ◽  
Reduced Glutathione ◽  
Cumene Hydroperoxide ◽  
Liver Homogenate ◽  
Thiobarbituric Acid ◽  
Albino Rats ◽  
Bitter Melon ◽  
Protective Effects

In the present study, protective effects of bitter melon (Momordica charantia) extract on lipid peroxidation induced by immobilization stress in rats have been assessed. Graded doses of extract (50, 100, and 150 mg/kg body weight) were administered orally to rats subjected to immobilization stress for two hours for seven consecutive days. Stress was applied by keeping the rats in a cage where no movement was possible. After seven days, rats were killed by decapitation after ether anesthesia. Blood and liver were collected to measure thiobarbituric acid reactive substances, reduced glutathione, and catalase. In vitro effects of M. charantia extract on lipid peroxidation in liver homogenate of normal, control, and rats pretreated with extract were carried out against cumene hydroperoxide-induced lipid peroxidation. Results reveal that in vivo M. charantia inhibited stress-induced lipid peroxidation by increasing the levels of reduced glutathione and activities of catalase. These results were further supported by in vitro results. In vitro inhibition of lipid peroxidation was indicated by low levels of thiobarbituric acid in the liver homogenate from pretreated rats and normal rats when incubated with both cumene hydroperoxide and extract. Inhibition was also noted in the homogenate where the rats were pretreated but the mixture contained no extract. Thus this plant provides protection by strengthening the antioxidants like reduced glutathione and catalase. Inclusion of this plant in the daily diet would be beneficial.

scholarly journals Aqueous Extracts of Teucrium polium Possess Remarkable Antioxidant Activity In Vitro

2006 ◽  
Vol 3 (3) ◽  
pp. 329-338 ◽  
Author(s):  
Predrag Ljubuncic ◽  
Suha Dakwar ◽  
Irina Portnaya ◽  
Uri Cogan ◽  
Hassan Azaizeh ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Rat Liver ◽  
Antioxidant Properties ◽  
Liver Homogenate ◽  
Plasma Oxidation ◽  
Teucrium Polium ◽  
Liver Homogenates ◽  
Β Carotene

Teucrium poliumL. (Lamiaceae) (RDC 1117) is a medicinal plant whose species have been used for over 2000 years in traditional medicine due to its diuretic, diaphoretic, tonic, antipyretic, antispasmodic and cholagogic properties. The therapeutic benefit of medicinal plants is often attributed to their antioxidant properties. We previously reported that an aqueous extract of the leaves and stems of this plant could inhibit iron-induced lipid peroxidation in rat liver homogenate at concentrations that were not toxic to cultured hepatic cells. Others have reported that organic extracts of the aerial components of this plant could inhibit oxidative processes. Against this background, we felt further investigation on the antioxidant action of the extract ofT. poliumprepared according to traditional Arab medicine was warranted. Accordingly, we assessed (i) its ability to inhibit (a) oxidation of β-carotene, (b) 2,2′-azobis(2-amidinopropan) dihydrochloride (AAPH)-induced plasma oxidation and (c) iron-induced lipid peroxidation in rat liver homogenates; (ii) to scavenge the superoxide ($${\hbox{ O }}_{2}^{\bullet -}$$) radical and the hydroxyl radical (OH•); (iii) its effects on the enzyme xanthine oxidase activity; (iv) its capacity to bind iron; and (v) its effect on cell glutathione (GSH) homeostasis in cultured Hep G2 cells. We found that the extract (i) inhibited (a) oxidation of β-carotene, (b) AAPH-induced plasma oxidation (c) Fe2+-induced lipid peroxidation in rat liver homogenates (IC50 = 7 ± 2 μg ml−1); (ii) scavenged $${\hbox{ O }}_{2}^{\bullet -}$$(IC50 = 12 ± 3 μg ml−1) and OH• (IC50 = 66 ± 20 μg ml−1); (iii) binds iron (IC50 = 79 ± 17 μg ml−1); and (iv) tended to increase intracellular GSH levels resulting in a decrease in the GSSG/GSH ratio. These results demonstrate that the extract prepared from theT. poliumpossesses antioxidant activityin vitro. Further investigations are needed to verify whether this antioxidant effect occursin vivo.

scholarly journals In-vitro and In-vivo Antioxidant Activity of Ethanol Leaf Extract of Justicia carnea

2020 ◽  
pp. 48-60
Author(s):  
Udedi Stanley Chidi ◽  
Ani Onuabuchi Nnenna ◽  
Asogwa Kingsley Kelechi ◽  
Maduji Fitzcharles Chijindu ◽  
Okafor Clinton Nebolisa
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Superoxide Dismutase ◽  
Leaf Extract ◽  
Dpph Radical ◽  
Β Carotene ◽  
In Vitro Antioxidant Activity

This study investigated the in-vitro antioxidant activity of ethanol leaf extract of Justicia carnea and its effect on antioxidant status of alloxan-induced diabetic albino rats. The in-vitro antioxidant activity was assayed by determining the total phenol, flavonoids, ascorbic acid, β-carotene and lycopene contents and by using 2,2 diphenyl-1-picrylhydrazyl (DPPH) radical, reducing antioxidant power and inhibition of lipid peroxidation antioxidant systems. Oxidative stress was produced in rats by single intraperitoneal injection of 150 mg/kg alloxan and serum concentration of malonaldehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) were determined. Five experimental groups of rats (n=6) were used for the study. Two groups of diabetic rats received oral daily doses of 100 and 200 mg/kg Justicia carnea leaf extract respectively while gilbenclamide (5 mg/ml); a standard diabetic drug was also given to a specific group for 14 days. From the result, the leaf extract contained a higher concentration of flavonoids followed byphenols, ascorbic acid, lycopene and β-carotene. The extract displayed more potent reducing power ability with EC50 of 40 µg/ml compared to BHA (EC50 of 400µg/ml). The percentage DPPH radical scavenging activity of the extract was also higher with EC50 of 200µg/ml and increased with increase in concentration while BHA had EC50of 320µg/ml. The inhibition of lipid peroxidation also increased with increase in concentration with EC50 of 58µg/ml and comparable with BHA (EC50=60µg/ml). The effect of the plant extract on antioxidant enzyme activities was concentration-dependent. Administration of 100mg/kg of the plant extract resulted in a significant decrease (p<0.05) in serum MDA concentration, while 200 mg/kg of the extract caused a significant (p˂0.05) increase in superoxide dismutase (SOD) and catalase activities with a non-significant increase (p>0.05) in the serum level of MDA when compared with the diabetic untreated group. These findings suggest that ethanol leaf extract of Justicia carnea have antioxidant properties and could handle diabetes-induced oxidative stress.

scholarly journals AMELIORATIVE EFFECTS OF STEM BARK OF THE WONDER TREE, PROSOPIS CINERARIA (L.) DRUCE AGAINST LPS-INDUCED TOXICITY: AN IN VITRO STUDY

2019 ◽  
pp. 96-102
Author(s):  
VEENA SHARMA ◽  
PREETI SHARMA
Keyword(s):  
Oxidative Stress ◽  
Plant Extract ◽  
Biochemical Parameters ◽  
Research Work ◽  
Liver Homogenate ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Prosopis Cineraria ◽  
Ethanol Plant

Objective: The present experimental investigation was planned to unravel and analyze the therapeutic potential of hydro-ethanol extract prepared from the stem bark of Prosopis cineraria against LPS-induced toxicity under in vitro conditions. Methods: Liver tissue samples from healthy Swiss albino male mice (Mus musculus) were used for the study. Liver homogenate (0.9 ml) was treated with 0.05 mg/ml of LPS along with 0.01 to 0.05 mg/ml of hydro-ethanol plant extract and allowed to incubate at 37˚C. The reactions were terminated at different time points at 0 min, 30 min, 1 h, 2 h, 4 h, 8 h and 24 h and alterations in oxidative stress (LPO, CAT, SOD, GSH, GST, and GPx) and biochemical parameters of hepatic toxicity (AST and ALT, ACP and ALP) were studied. Results: The results demonstrated that the obliterations in the levels of oxidative and biochemical parameters due to LPS induced toxicity were restored by the treatment with hydro-ethanol extract of Prosopis cineraria under in vitro conditions. The altered levels were biochemical parameters were observed at 0.05 mg/ml LPS concentration after 2 h; but administration of hydro-ethanol plant extract at concentration 0.04 mg/ml effectively reduced its level when compared to LPS treated samples under in vitro conditions Conclusion: The present research work unravelled the alleviating potential of a hydro-ethanol extract of Prosopis cineraria against LPS-induced toxicity by combating oxidative stress under in vitro environment.

scholarly journals Intestinal Anti-Inflammatory Activity ofBaccharis dracunculifoliain the Trinitrobenzenesulphonic Acid Model of Rat Colitis

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Sílvia Helena Cestari ◽  
Jairo Kennup Bastos ◽  
Luiz Claudio Di Stasi
Keyword(s):  
Lipid Peroxidation ◽  
Plant Extract ◽  
Inflammatory Activity ◽  
Glutathione Depletion ◽  
Phytochemical Analysis ◽  
Coumaric Acid ◽  
Brain Membrane ◽  
Anti Inflammatory ◽  
Improve Health

Baccharis dracunculifoliaDC (Asteraceae) is a Brazilian medicinal plant popularly used for its antiulcer and anti-inflammatory properties. This plant is the main botanical source of Brazilian green propolis, a natural product incorporated into food and beverages to improve health. The present study aimed to investigate the chemical profile and intestinal anti-inflammatory activity ofB. dracunculifoliaextract on experimental ulcerative colitis induced by trinitrobenzenosulfonic acid (TNBS). Colonic damage was evaluated macroscopically and biochemically through its evaluation of glutathione content and its myeloperoxidase (MPO) and alkaline phosphatase activities. Additionalin vitroexperiments were performed in order to test the antioxidant activity by inhibition of induced lipid peroxidation in the rat brain membrane. Phytochemical analysis was performed by HPLC using authentic standards. The administration of plant extract (5 and 50 mg kg−1) significantly attenuated the colonic damage induced by TNBS as evidenced both macroscopically and biochemically. This beneficial effect can be associated with an improvement in the colonic oxidative status, since plant extract prevented glutathione depletion, inhibited lipid peroxidation and reduced MPO activity. Caffeic acid,p-coumaric acid, aromadendrin-4-O-methyl ether, 3-prenyl-p-coumaric acid, 3,5-diprenyl-p-coumaric acid and baccharin were detected in the plant extract.

Attenuated effects of peptides derived from porcine plasma albumin on in vitro lipid peroxidation in the liver homogenate of mice

2008 ◽  
Vol 111 (2) ◽  
pp. 364-369 ◽  
Author(s):  
Hao Zhang ◽  
Jinzhi Wang ◽  
Ran Li ◽  
Jing Bai ◽  
Yubin Ye ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Liver Homogenate ◽  
Plasma Albumin

scholarly journals Aquaporin-3 regulates endosome-to-cytosol transfer via lipid peroxidation for cross presentation

PLoS ONE ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. e0238484
Author(s):  
Sam C. Nalle ◽  
Rosa Barreira da Silva ◽  
Hua Zhang ◽  
Markus Decker ◽  
Cecile Chalouni ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Mhc Class I ◽  
Cd8 T Cells ◽  
Membrane Integrity ◽  
Protein Antigens ◽  
Aquaporin 3 ◽  
Viral Response ◽  
Cross Presentation

Antigen cross presentation, whereby exogenous antigens are presented by MHC class I molecules to CD8+ T cells, is essential for generating adaptive immunity to pathogens and tumor cells. Following endocytosis, it is widely understood that protein antigens must be transferred from endosomes to the cytosol where they are subject to ubiquitination and proteasome degradation prior to being translocated into the endoplasmic reticulum (ER), or possibly endosomes, via the TAP1/TAP2 complex. Revealing how antigens egress from endocytic organelles (endosome-to-cytosol transfer, ECT), however, has proved vexing. Here, we used two independent screens to identify the hydrogen peroxide-transporting channel aquaporin-3 (AQP3) as a regulator of ECT. AQP3 overexpression increased ECT, whereas AQP3 knockout or knockdown decreased ECT. Mechanistically, AQP3 appears to be important for hydrogen peroxide entry into the endosomal lumen where it affects lipid peroxidation and subsequent antigen release. AQP3-mediated regulation of ECT was functionally significant, as AQP3 modulation had a direct impact on the efficiency of antigen cross presentation in vitro. Finally, AQP3-/- mice exhibited a reduced ability to mount an anti-viral response and cross present exogenous extended peptide. Together, these results indicate that the AQP3-mediated transport of hydrogen peroxide can regulate endosomal lipid peroxidation and suggest that compromised membrane integrity and coordinated release of endosomal cargo is a likely mechanism for ECT.

Protective effects of casein-derived peptide VLPVPQK against hydrogen peroxide–induced dysfunction and cellular oxidative damage in rat osteoblastic cells

2017 ◽  
Vol 36 (9) ◽  
pp. 967-980 ◽  
Author(s):  
SB Mada ◽  
S Reddi ◽  
N Kumar ◽  
S Kapila ◽  
R Kapila
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Oxidative Damage ◽  
Osteoblastic Cells ◽  
Antioxidant Enzyme Activities ◽  
Protective Agent ◽  
Protective Effects ◽  
Differentiation Markers

Oxidative stress inhibits osteoblast differentiation and function that lead to the development of osteoporosis. Casein-derived peptide VLPVPQK (PEP), a potent antioxidant, was isolated from β-casein of buffalo milk. We used an in vitro oxidative stress model induced by hydrogen peroxide (H2O2) in rat osteoblastic cells to investigate the protective effects of PEP against H2O2-induced dysfunction and oxidative damage. Cells were pretreated with PEP (50–200 ng/mL) for 2, 7 or 21 days followed by 0.3 mM H2O2 treatment for 24 h and then markers of osteogenic development, oxidative damage and apoptosis were examined. PEP significantly increased the viability and differentiation markers of osteoblast cells such as alkaline phosphatase and calcium mineralization. Moreover, PEP suppressed the production of reactive oxygen species (ROS), lipid peroxidation and ameliorated H2O2-induced reduction in glutathione, superoxide dismutase and catalase activities. In addition, PEP partially inhibited caspase-9 and-3 activities and reduced propidium iodide–positive cells. Altogether, our results demonstrated that PEP could protect rat osteoblast against H2O2-induced dysfunction and oxidative damage by reduction of ROS production, lipid peroxidation and increased antioxidant enzyme activities. Thus, our data suggest that PEP might be a valuable protective agent against oxidative stress–related diseases such as osteoporosis.

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