Regulation of Prostaglandin F2α-Receptor mRNA in Human Granulosa-Luteal Cells by Human Chorionic Gonadotrophin and Prostaglandin F2α

Endocrine ◽  
1998 ◽  
Vol 8 (3) ◽  
pp. 261-268 ◽  
Author(s):  
Jeffrey E. Väänänen ◽  
Céline C. M. Väänänen ◽  
Suzie Lee ◽  
Basil Ho Yuen ◽  
Peter C. K. Leung
Keyword(s):  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Luteal Cells ◽  
Receptor Mrna ◽  
Prostaglandin F

Oxytocin may play a role in the control of the human corpus luteum

1982 ◽  
Vol 95 (1) ◽  
pp. 65-70 ◽  
Author(s):  
G. J. S. Tan ◽  
R. Tweedale ◽  
J. S. G. Biggs
Keyword(s):  
Menstrual Cycle ◽  
Corpus Luteum ◽  
Inhibitory Action ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Corpora Lutea ◽  
Luteal Cells ◽  
Progesterone Production ◽  
Human Corpus Luteum

The effects of oxytocin on dispersed luteal cells from human corpora lutea of the menstrual cycle were studied. Oxytocin at a concentration of 4 mi.u./ml produced a slight increase in basal progesterone production. However, higher oxytocin concentrations (400 and 800 mi.u./ml) markedly inhibited both basal and human chorionic gonadotrophin-induced progesterone production. These data provide evidence for an effect of oxytocin on the human corpus luteum. In view of the inhibitory action of oxytocin, increased secretion of this hormone may be important in the demise of the corpus luteum at the end of the menstrual cycle.

CORPUS LUTEUM ACTIVITY AND PROSTAGLANDIN LEVELS AFTER PARTURITION IN COWS WITH RETAINED FETAL MEMBRANES

1987 ◽  
Vol 67 (1) ◽  
pp. 21-26 ◽  
Author(s):  
PIERRE MATTON ◽  
VICTOR ADELAKOUN ◽  
JACQUES DUFOUR
Keyword(s):  
Key Words ◽  
Corpus Luteum ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Fetal Membranes ◽  
Plasma Progesterone ◽  
Blood Samples ◽  
Retained Placenta ◽  
Progesterone Production ◽  
Prostaglandin F

Previous results have shown that progesterone levels were higher on the day of parturition in cows with retained fetal membranes (RFM) than in cows with normal calving, suggesting incomplete lysis of the corpus luteum (CL). This experiment was performed to evaluate the activity of the CL and the level of 13,14-dihydro-15-keto prostaglandin F2α (PGFM) in RFM cows. Cows with RFM or those calving normally (NC) were ovariectomized 12–14 h after parturition. Blood samples were taken from the caudal and utero-ovarian veins. Slices of CL were incubated with or without human chorionic gonadotrophin (hCG) medium for 3 h. Plasma progesterone was higher in both the caudal and utero-ovarian veins of RFM cows than in those of NC cows (1.12 ± 0.25 vs. 0.62 ± 0.08 ng mL−1 and 2.4 ± 0.3 vs. 1.44 ± 0.33 ng mL−1, respectively). PGFM was also significantly higher in RFM cows (3.62 ± 0.19 vs. 2.55 ± 0.15 ng mL−1). Progesterone production by CL slices from both types of cows, incubated without hCG, was similar (65 ± 4.2 vs. 73 ± 5.1 μg g−1); with hCG, however, the progesterone production by the CL of RFM cows was 186.3 ± 10.7 μg g−1, 75.7 μg g−1 more than in CL of cows with normal calving. These results support the hypothesis of an incomplete luteolysis of the CL in RFM cows in spite of hieher levels of PGF2α. Key words: Corpus luteum activity, progesterone, prostaglandin, postpartum cows, retained placenta

Effects of prolactin on steroid production by human luteal cells in vitro

1983 ◽  
Vol 96 (3) ◽  
pp. 499-503 ◽  
Author(s):  
G. J. S. Tan ◽  
J. S. G. Biggs
Keyword(s):  
Menstrual Cycle ◽  
Ovarian Function ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Corpora Lutea ◽  
Short Term ◽  
Steroid Production ◽  
Luteal Cells ◽  
Progesterone Production

The effects of prolactin on steroidogenesis were studied in dispersed luteal cells prepared from human corpora lutea of the menstrual cycle. Prolactin, at concentrations of 0·1–1000 ng/ml, had no effect on progesterone production by luteal cells during short-term incubation (3 h). However, in two out of five corpora lutea, higher concentrations of prolactin (100 and 1000 ng/ml) significantly reduced the oestradiol-17β production induced by human chorionic gonadotrophin (hCG; 10 i.u./ml); lower doses of prolactin had little effect. In the remaining corpora lutea, prolactin failed to affect either basal or hCG-induced production of oestradiol-17β. These results are discussed in relation to the mechanism by which prolactin influences human ovarian function.

INFLUENCE OF PROSTAGLANDINS AND HUMAN CHORIONIC GONADOTROPHIN ON PROGESTERONE CONCENTRATION AND OOCYTE MATURATION IN MOUSE OVARIAN FOLLICLES MAINTAINED IN ORGAN CULTURE

1975 ◽  
Vol 65 (1) ◽  
pp. 19-25 ◽  
Author(s):  
P. NEAL ◽  
T. G. BAKER ◽  
K. P. McNATTY ◽  
R. J. SCARAMUZZI
Keyword(s):  
Organ Culture ◽  
Culture Medium ◽  
Control Level ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Prostaglandin E ◽  
Progesterone Concentration ◽  
Histological Changes ◽  
Control Value ◽  
Prostaglandin F

SUMMARY The response of mouse ovaries maintained in organ culture to prostaglandin E2 (PGE2) and prostaglandin F2α (F2α) was assessed using quantitative histological and radioimmunoassay procedures. Prostaglandin E2 induced histological changes in the cultured follicles comparable to those induced by human chorionic gonadotrophin (HCG) and the increase in the number of oocytes undergoing preovulatory maturation over the control value was the same irrespective of the treatment (PGE2 alone, HCG alone, or PGE2 + HCG). The amount of progesterone/ ml of culture medium was also significantly higher with these preparations than in control cultures (about 125 ng/ml compared with 57 ng/ml). By contrast, 5 μg PGF2α/ml medium increased neither the number of oocytes undergoing maturation nor the concentration of progesterone in the culture medium. The latter increased when the dose of PGF2α was increased to 30 μg/ml, although the proportion of oocytes beyond the dictyate stage remained at the control level. There was no augmentation in the response (above the level for HCG alone) when HCG and PGF2α were added to the explant medium simultaneously. These results are discussed in terms of the possible mechanism of action of the various preparations.

Inhibitory action of chemically deglycosylated human chorionic gonadotrophin on hormone-induced steroid production by dispersed cells from human corpus luteum

1984 ◽  
Vol 101 (3) ◽  
pp. 327-332 ◽  
Author(s):  
M. C. Richardson ◽  
G. M. Masson ◽  
M. R. Sairam
Keyword(s):  
Corpus Luteum ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Maximum Response ◽  
Luteal Cells ◽  
Progesterone Production ◽  
Anhydrous Hydrogen Fluoride ◽  
Tenfold Excess ◽  
Human Corpus Luteum ◽  
Dispersed Cells

ABSTRACT The biological activity of deglycosylated human chorionic gonadotrophin (hCG) prepared by treatment of the native hormone with anhydrous hydrogen fluoride was evaluated using suspensions of dispersed cells from biopsies of human corpus luteum obtained during the luteal phase of normal menstrual cycles. A reproducible pattern of response to hCG in terms of progesterone production by luteal cells was established for a range of luteal ages. Deglycosylation of hCG led to a diminished level of maximum response to the hormone. Co-incubation of luteal cells with a level of hCG just sufficient to elicit a maximum response and increasing concentrations of deglycosylated hCG led to a progressive inhibition of the hormonal response; at a concentration of 103 ng deglycosylated hCG/ml (a tenfold excess of deglycosylated hCG over the native hormone), hCG-induced progesterone production was reduced by about 50%. Deglycosylated hCG therefore acts as a partial antagonist for the action of hCG on human luteal cells. J. Endocr. (1984) 101, 327–332

Control of inhibin production by dispersed human luteal cells in vitro

1992 ◽  
Vol 4 (1) ◽  
pp. 67 ◽  
Author(s):  
HZ Wang ◽  
SH Lu ◽  
XJ Han ◽  
ZD Sun ◽  
WX Shen ◽  
...  
Keyword(s):  
Sex Steroids ◽  
Follicle Stimulating Hormone ◽  
Calf Serum ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Corpora Lutea ◽  
Luteal Cells ◽  
Oestradiol Production ◽  
Human Corpus Luteum

The production of inhibin in vitro by dispersed cells from early to mid (Days 16-19) and late stage (Day 23) human corpus luteum (CL) was examined, and the effects of human chorionic gonadotrophin (hCG), follicle stimulating hormone (FSH), oestradiol and testosterone on inhibin production were determined. Corpora lutea from five subjects in the early to mid luteal stage and three subjects in late luteal stage were dispersed with enzymes and the luteal cells cultured in medium supplemented with 5% calf serum and either FSH (1, 10 or 100 ng mL-1), oestradiol-17 beta (2.5, 5 or 10 micrograms mL-1) or testosterone (0.25, 1 or 5 micrograms mL-1) with or without hCG (1 I.U. mL-1). Cells were cultured for 1 to 3 days without changes of medium, and the concentrations of progesterone, oestradiol and immunoreactive inhibin in the medium were measured by radioimmunoassay. Cells from both types of CL produced inhibin in vitro under basal conditions, but only cells from early to mid CLs responded to hCG with a significant increase in inhibin production. Both progesterone and oestradiol production were stimulated by hCG in both groups of CL. Inhibin concentrations in the cell cultures declined with time in culture, particularly in the late CL group, whereas the concentration of steroids increased. Neither FSH, oestradiol nor testosterone significantly changed inhibin production in either CL group. It was concluded that inhibin production by human luteal cells in vitro is influenced by the age of the CL, and is dependent on LH (hCG) but not on FSH or sex steroids.

Luteal steroidogenesis and regression in the rat: Effects of human chorionic gonadotrophin and phospholipase A2 on cells and plasma membranes

1990 ◽  
Vol 125 (3) ◽  
pp. 387-396 ◽  
Author(s):  
E. A. Greenhalgh
Keyword(s):  
Membrane Fluidity ◽  
Fluorescence Polarization ◽  
Plasma Membranes ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Luteal Cell ◽  
Free Radical Damage ◽  
Anionic Phospholipids ◽  
Prostaglandin F

ABSTRACT Progesterone production in vitro was measured in response to human chorionic gonadotrophin and phospholipase A2 using dispersed luteal cells from control and prostaglandin F2α (PGF2α)-treated rats. Highly purified luteal plasma membrane suspensions were prepared by partition distribution in dextranpolyethylene glycol, and membrane fluidity was measured by fluorescence polarization using the probe, trans-parinaric acid. Fluorescence polarization was highly dependent upon calcium, and was markedly affected by PGF2α treatment. No support was found for the theory that bulk membrane fluidity has a role in receptor or enzyme hindrance in the induction of luteal cell regression. However, membrane phase transitions induced by calcium interaction with anionic phospholipids and free radical damage may be involved in regression of the rat corpus luteum. Journal of Endocrinology (1990) 125, 387–396

PROLACTIN AND THE REGULATION OF 20α-DIHYDROPROGESTERONE SECRETION OF THE SUPER-LUTEINIZED RAT OVARY DURING LUTEOLYSIS INDUCED BY A PROSTAGLANDIN F2α ANALOGUE

1978 ◽  
Vol 87 (3) ◽  
pp. 625-631 ◽  
Author(s):  
P. A. Torjesen ◽  
R. Dahlin ◽  
E. Haug ◽  
A. Aakvaag
Keyword(s):  
Prostaglandin F2α ◽  
Serum Levels ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Female Rats ◽  
Serum Progesterone ◽  
Prostaglandin F ◽  
Rat Ovary ◽  
Pre Treatment ◽  
Observation Period

ABSTRACT Twenty-five day old female rats were treated with pregnant mare's serum gonadotrophin (PMSG) and human chorionic gonadotrophin (HCG) to achieve a state of luteinization. Eight days after the HCG administration luteolysis was induced by a subcutaneous injection of 5 μg of the prostaglandin F2α analogue cloprostenol (Estrumate®, ICI 80996). In animals treated with 2-Br-α-ergocryptine (BEC), administration of cloprostenol decreased serum progesterone levels from 580 to 20 ng/ml in 5 h and progesterone remained low for the next 18 h. The serum levels of 20α-dihydroprogesterone (20α-DHP) and prolactin (PRL) remained at pre-treatment values (20α-DHP 85 to 170 ng/ml; PRL less than 5 ng/ml) throughout the observation period. When animals treated with both BEC and cloprostenol were given PRL 5 h after the prostaglandin injection, an increased 20α-DHP level (630 ng/ml) was found 23 h after the cloprostenol administration, while the progesterone level was decreased (70 ng/ml). These findings were similar to the observations following cloprostenol treatment alone. The study indicates a causal relationship between the increase in the serum levels of PRL and 20α-DHP observed after PGF2α induced luteolysis in rats with superluteinized ovaries.

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