scholarly journals Comparison of Two Spectrophotometric Methods for Quantifying Total Hydroxycinnamic Acids in Coneflower (Echinacea purpurea) Preparations

2018 ◽  
Vol 8 (4) ◽  
pp. 235-242
Author(s):  
Hossein Mahdavi ◽  
Mohammad Reza Naderi ◽  
Mohammad Hossein Esmaeili
Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1148
Author(s):  
Veronica Vendramin ◽  
Alessia Viel ◽  
Simone Vincenzi

Phenolic acids represent about one-third of the dietary phenols and are widespread in vegetable and fruits. Several plants belonging to both vegetables and medical herbs have been studied for their hydroxycinnamic acid content. Among them, Echinacea purpurea is preferentially used for caffeic acid-derivatives extraction. The wine industry is a source of by-products that are rich in phenolic compounds. This work demonstrates that unripe grape juice (verjuice) presents a simple high-pressure liquid chromatography (HPLC) profile for hydroxycinnamic acids (HCAs), with a great separation of the caffeic-derived acids and a low content of other phenolic compounds when compared to E. purpurea and other grape by-products. Here it is shown how this allows the recovery of pure hydroxycinnamic acids by a simple and fast method, fast protein liquid chromatography (FPLC). In addition, verjuice can be easily obtained by pressing grape berries and filtering, thus avoiding any extraction step as required for other vegetable sources. Overall, the proposed protocol could strongly reduce the engagement of solvent in industrial phenolic extraction.


Proceedings ◽  
2018 ◽  
Vol 2 (25) ◽  
pp. 1528
Author(s):  
Burak Durmaz ◽  
Latife Merve Oktay ◽  
Hikmet Mehmedov ◽  
Nur Selvi Günel ◽  
Hatice Kalkan Yıldırım ◽  
...  

: In this study, the fruits and leaves of wild blueberries grown naturally in our country will be evaluated by using different infusion and boiling methods. Blueberry teas; leaves, raw fruit, dried and shredded raw fruit, fruit beans and seedless raw fruit of different infusions were used after boiling them for 1 min, 3 min, 5 min, 7 min, 10 min. Phenolic levels were determined by LC MS/MS technique. The antioxidant and activities of all products in the vitro HCT-116 colon cancer cell line were analyzed by spectrophotometric methods. MDA and TEAC were evaluated for antioxidant activity. Cytotoxicity and viability tests were performed by adding WST-8 (Water Soluble Tetrazolium Salt-8) solution. For apoptosis, TRAIL and Apaf-1 ELISA Kit were used for the activation of caspases of intrinsic and extrinsic pathways.


Planta Medica ◽  
2013 ◽  
Vol 79 (05) ◽  
Author(s):  
MT Khosravi ◽  
A Mehrafarin ◽  
H Naghdibadi ◽  
E Khosravi

2005 ◽  
Vol 26 (1) ◽  
pp. 20-21
Author(s):  
Volker Schulz
Keyword(s):  

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