scholarly journals KARAKTERISASI PISANG REJANG TETRAPLOID HASIL INDUKSI DENGAN ORYZALIN

2017 ◽  
Vol 16 (1) ◽  
Author(s):  
Yuyu Suryasari Poerba ◽  
T Handayani ◽  
Witjaksono Witjaksono
Keyword(s):  
Molecular Characterization ◽  
Ploidy Level ◽  
Issr Markers ◽  
Musa Acuminata ◽  
Diploid Cultivar ◽  
Banana Industry ◽  
Ploidy Identification ◽  
Rapd And Issr ◽  
Banana Cultivar

Triploid banana cultivar is the most desirable cultivar in banana industry because of its higher yield compared to its diploid cultivar.  The  triploid cultivar can be produced by crossing tetraploid with diploid cultivar.  However, tetraploid banana cultivar is rarely existed naturally.  Induced tetraploid of Pisang Rejang was produced using oryzalin. The present research was conducted to characterize tetraploid Pisang Rejang (Musa acuminata, AAAA genome) induced by in-vitro oryzalin treatment from diploid Pisang Rejang. Ploidy level, molecular and morphotaxonomic characters were observed.  Ploidy identification of induced Pisang Rejang was conducted using Flowcytometer.  Molecular characterization was done using RAPD and ISSR markers. Morphology characters were observed based on UPOV (2010). The results showed that tetraploid plants have similar genetic properties with their diploid controls as shown by genetic identity of 0.9901 – 0.9935. The tetraploids were differed from their diploid plants in plan habit and diameter of fruit.  The tetraploid plants produce fewer suckers, drooping leaves and broader fruits compared to its diploid control.

Molecular characterization of jasmine genotypes using RAPD and ISSR markers

2020 ◽  
Vol 77 (1) ◽  
pp. 149
Author(s):  
Sanchita Ghosh ◽  
M. Ganga ◽  
K. Soorianathasundaram ◽  
Ajit Kumar
Keyword(s):  
Molecular Characterization ◽  
Issr Markers ◽  
Rapd And Issr

scholarly journals Polyploidy induction in Phlox paniculata L. under in vitro conditions

2010 ◽  
Vol 58 (1) ◽  
pp. 101-106 ◽  
Author(s):  
Pavel Matiska ◽  
Hana Vejsadová
Keyword(s):  
Flow Cytometry ◽  
Ploidy Level ◽  
Chemical Mutagen ◽  
Nutrient Media ◽  
Phlox Paniculata ◽  
Diploid Cultivar ◽  
Infiltration Method

The objective of this work was to find an effective method of polyploidy induction using chemomutagens, colchicine and oryzalin, in diploid cultivar of Phlox paniculata ‘Fujiyama’ (syn. Mt. Fuji, Fuji). Ploidy level was determined by the flow cytometry method (FCM). Two methods of treating the explants (in vitro regenerated shoots) were tested; chemomutagen infiltration from nutrient media (“the infiltration method”) and dipping of the explants in a chemical mutagen solution (“the dip method”). The highest values of tetraploid (5%), mixoploid (1.67%) frequency and polyploidization efficiency (1.25) were found in explants treated with 0.2% colchicine for 24 h in the dip method. Concentrations of 10 µM oryzalin and 0.2% colchicine for 14 d were the most effective for obtaining tetraploids in the infiltration method. The results will be exploited to other P. paniculata cultivars for breeding of this species.

Genetic stability using RAPD and ISSR markers in efficiently in vitro regenerated plants of Inula royleana DC

Meta Gene ◽  
2018 ◽  
Vol 18 ◽  
pp. 100-106 ◽  
Author(s):  
Samar Amin ◽  
Tareq A. Wani ◽  
Zahoor Ahmad Kaloo ◽  
Seema Singh ◽  
Riffat John ◽  
...  
Keyword(s):  
Genetic Stability ◽  
Issr Markers ◽  
Regenerated Plants ◽  
Rapd And Issr

Molecular Characterization of Brinjal (Solanum melongena L) Cultivars using RAPD and ISSR Markers

2009 ◽  
Vol 18 (2) ◽  
pp. 189-195 ◽  
Author(s):  
Shailesh K. Tiwari ◽  
J. L. Karihaloo ◽  
Nowsheen Hameed ◽  
Ambika B. Gaikwad
Keyword(s):  
Molecular Characterization ◽  
Solanum Melongena ◽  
Issr Markers ◽  
Rapd And Issr

scholarly journals Molecular Characterization of Saffron-Potential Candidates for Crop Improvement

2015 ◽  
Vol 7 (1) ◽  
pp. 81-89 ◽  
Author(s):  
Javid Iqbal MIR ◽  
Nazeer AHMED ◽  
Mudasir Hafiz KHAN ◽  
Taseem Ahmad MOKHDOMI ◽  
Sajad Hussian WANI ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Ssr Markers ◽  
Significant Variation ◽  
Rapd Markers ◽  
Crop Improvement ◽  
Issr Markers ◽  
Similarity Coefficient ◽  
Improvement Programs ◽  
Rapd And Issr

In this study, thirty one (31) morphologically distinct selections of saffron crop were used for molecular characterization. Molecular characterization was done through SSR, ISSR and RAPD markers. RAPD and ISSR markers showed significant variation; however, SSR markers did not reveal any variation between the selected clones. The Jaccard’s similarity coefficient ranged from 0.94 to 1.00 with an average of 0.98 among all 31 selections used. Minimum similarity value (0.94) was observed between CITH-S-107 and PAM-S-116 selections. The study provides sufficient knowledge to identify clones with better stigma characteristics for further crop improvement programs.

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