scholarly journals Contemporary interspecific hybridization between Dracocephalum kotschyi and Dracocephalum oligadenium (Lamiaceae): Evidence from morphological, anatomical and molecular data

2019 ◽  
Vol 62 (2) ◽  
pp. 123-129 ◽  
Author(s):  
Fahimeh Koohdar ◽  
Farideh Attar ◽  
Seyed Mehdi Talebi ◽  
Masoud Sheidai
Keyword(s):  
Simple Sequence Repeat ◽  
Molecular Data ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Tropical Asia ◽  
Dracocephalum Kotschyi ◽  
The Family ◽  
Anatomical Characters ◽  
Simple Sequence ◽  
Important Medicinal Plant

Dracocephalum is the second largest genus in the family Lamiaceae with about 186 species. These species are native in temperate regions of the Northern Hemisphere and occur in the territory of the extra-tropical Asia and Europe. Eight Dracocephalum species reported in Iran; these are mainly growing in the northern and central parts of the country belonging to the Irano-Turanian phytogeographical region. Dracocephalum kotschyi is an important medicinal plant .in the country. At the same time, taxonomic position of Dracocephalum oligadenium is a challenging issue. In this work morphological, anatomical and Inter Simple Sequence Repeat (ISSR) markers were used to identify these species in Iran. MDS plot based on morphological and anatomical characters, furthermore, PCoA and MST plot based on ISSR data of species revealed hybridization between D. oligadenium and D. kotschyi.

scholarly journals Association of Morphological, Ecological, and Genetic Diversity of Aerva javanica Populations Growing in the Eastern Desert of Egypt

Agronomy ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 402
Author(s):  
Noha A. El-Tayeh ◽  
Hanaa K. Galal ◽  
Magda I. Soliman ◽  
Hoida Zaki
Keyword(s):  
Morphological Diversity ◽  
Eastern Desert ◽  
Molecular Data ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Soil Variables ◽  
Sustainable Conservation ◽  
Simple Sequence ◽  
Important Medicinal Plant ◽  
Issr Primers

Aerva javanica is one of Egypt’s most important traditional medicinal plants used as antidiarrheal and anthelmintic medicine and recently as an anticancer agent. In this study, variations among ten populations of Aerva javanica in different sites in the Eastern Desert of Egypt were analyzed based on morphological and ecological attributes and molecular variation expressed by Inter-Simple Sequence Repeat (ISSR) markers. Morphological diversity was higher for populations in the Wadi El-Markh and Bir Abbady regions than others. The polymorphism revealed by ten ISSR primers was 79.4% among populations. Distance trees created using the results obtained from soil variables, morphological characterizations, and molecular data showed that the highest similarity was 0.974 between Populations 8 and 9, while the lowest similarity was 0.715 between Population 1 and Population 3 regions. In conclusion, the obtained data are important to design a plan for sustainable conservation of Aerva javanica as an important medicinal plant having a wide interspecific genetic variability within various populations.

Genetic variation among Spanish pea landraces revealed by Inter Simple Sequence Repeat (ISSR) markers: its application to establish a core collection

2006 ◽  
Vol 144 (1) ◽  
pp. 53-61 ◽  
Author(s):  
A. LÁZARO ◽  
I. AGUINAGALDE
Keyword(s):  
Simple Sequence Repeat ◽  
Core Collection ◽  
Genetic Relationships ◽  
Plant Genetic Resources ◽  
Molecular Data ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Sequence Repeat ◽  
Data Types ◽  
Simple Sequence

One hundred and twenty Spanish landraces of peas (Pisum sativum L.) collected in different micro-climatic areas, together with three commercial varieties and one breeding line, were subjected to molecular characterization using Inter Simple Sequence Repeat (ISSR) amplification. Landraces originated from 7 to 1427 m asl. Most accessions were collected in temperate areas with dry Mediterranean humidity regimes. ISSR markers detected high levels of variability between accessions and were useful for identification of individuals and clarification of genetic relationships between them. The 18 groups obtained with molecular data were not significantly related to 21 agro/climatic regions. Clustering based on climatic and ISSR characters differed because the data types present different aspects of genetic diversity. These results will be applied to establish a pea core collection at the Plant Genetic Resources Centre of the Spanish National Institute of Agriculture and Food Research and Technology (CRF-INIA).

scholarly journals Screening of homokaryotic protoclones of Agaricus bisporus (J. Lge) Imbach by colony characters and ISSR markers

2010 ◽  
Vol 39 (1) ◽  
pp. 119-122 ◽  
Author(s):  
Mahmudul Islam Nazrul ◽  
Fan Xiao Lin ◽  
Bian Yin-Bing
Keyword(s):  
Simple Sequence Repeat ◽  
Agaricus Bisporus ◽  
Morphological Characters ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Fruiting Bodies ◽  
Sequence Repeat ◽  
Slow Growing ◽  
Simple Sequence ◽  
Issr Primers

Among ten slow-growing protoclones of Agaricus bisporus (J. Lge) Imbach, all appressed colonies showed slower growth rate and spawn run, and inability to produce fruiting bodies in substrate. Seven of 40 inter-simple sequence repeat (ISSR) primers amplified 78 reproducible fragments, 48.93% were polymorphic, each producing 7 to 16 bands ranging from 0.10 to 2.10 kbp, sufficient to differentiate the protoclones from each other. Appressed protoclones were homoallelic at a number of loci that were heteroallelic in the parent, suggesting that they represented rare homokaryons. Thus, using morphological characters along with ISSR, polymorphisms could be useful for quick, easy, and accurate in distinguishing homo- and heterokaryotic isolates. Key words: Agaricus bisporus (J. Lge) Imbach; Homokaryon; ISSR; Protoclone DOI: 10.3329/bjb.v39i1.5537Bangladesh J. Bot. 39(1): 119-122, 2010 (June)

scholarly journals Genetic diversity and interspecific relationships of some Allium species using inter simple sequence repeat markers

2015 ◽  
Vol 22 (2) ◽  
pp. 67-75 ◽  
Author(s):  
Leila Samiei ◽  
Mahnaz Kiani ◽  
Homa Zarghami ◽  
Farshid Memariani ◽  
Mohammad Reza Joharchi
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeat ◽  
Gene Diversity ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Group Method ◽  
Sequence Repeat ◽  
Allium Species ◽  
Interspecific Relationships ◽  
Simple Sequence

In this study genetic diversity and interspecific relationships of 11 Allium L. species from Khorassan province of Iran including 32 accessions were investigated by inter simple sequence repeat (ISSR) markers. Nine ISSR primers produced a total of 80 polymorphic markers and revealed high polymorphism among the studied species. The average gene diversity, effective number of alleles and Shannon’s information index were 0.2, 1.28 and 0.3, respectively. Allium kuhsorkhense exhibited the greatest level of variation (He: 0.18), whereas A. stipitatum demonstrated the lowest level of variability (He: 0.05). UPGMA (Unweighted Pair Group Method with Arithmetic mean) analysis showed that Allium accessions have a similarity range of 0.60 to 0.95. Allium scapriscapum composed the most distant group in the dendrogram. The clustered groups of Allium species clearly reflect the recent taxonomic concept of the genus at the subgenus and section levels. The present study showed that the ISSR technique is an effective molecular approach for analyzing genetic diversity and relationship in Allium species.Bangladesh J. Plant Taxon. 22(2): 67-75, 2015 (December)

Extent of clonality, genetic diversity and decline in the endangered mallee Eucalyptus imlayensis

2007 ◽  
Vol 55 (5) ◽  
pp. 548 ◽  
Author(s):  
Elizabeth A. James ◽  
Keith L. McDougall
Keyword(s):  
Simple Sequence Repeat ◽  
Ex Situ Conservation ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Lower Number ◽  
Ex Situ ◽  
Sequence Repeat ◽  
Eastern Australia ◽  
Number Of Stems ◽  
Simple Sequence

Eucalyptus imlayensis Crisp & Brooker is a rare mallee known from one location in south-eastern Australia. Discovered in 1977, the population has declined in number and health of stems since 1998. Inter-simple sequence repeat (ISSR) markers were used to assess genetic variation and clonality. Only five multilocus genotypes were distinguished from 27 samples and the proximity of like genotypes within the population is consistent with the population being largely clonal. This means that the species has a much lower number of genetic individuals than is suggested from a census of the number of stems present. The implications of this finding for ex situ conservation of the species are discussed.

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