The Bioenhancing Properties of Piper guineense Using Microbiological Assay Method

2013 ◽  
Vol 1 (3) ◽  
pp. 93
Author(s):  
Sunday O Nduka ◽  
Onyinyechi Asadu ◽  
Sonnie Ikechukwu Mbagwu ◽  
Matthew J Okonta
Keyword(s):  
Microbiological Assay ◽  
Assay Method ◽  
Piper Guineense

scholarly journals A MICROBIOLOGICAL ASSAY METHOD FOR p-AMINOBENZOIC ACID

1943 ◽  
Vol 148 (2) ◽  
pp. 281-287
Author(s):  
Roy C. Thompson ◽  
Edith R. Isbell ◽  
Herschel K. Mitchell
Keyword(s):  
Microbiological Assay ◽  
Assay Method ◽  
Aminobenzoic Acid

Collaborative Study of Turbidimetric Microbiological Assay of Monensin in Chicken Rations

1977 ◽  
Vol 60 (1) ◽  
pp. 179-182
Author(s):  
H Latham Breunig ◽  
Robert E Scroggs ◽  
Lealon V Tonkinson ◽  
Henry Bikin
Keyword(s):  
Experimental Design ◽  
Collaborative Study ◽  
Microbiological Assay ◽  
Assay Method ◽  
Turbidimetric Method ◽  
Automated Method ◽  
Relative Standard ◽  
Significant Difference ◽  
Feed Form ◽  
Automated Methods

Abstract A turbidimetric microbiological assay method for monensin in chicken rations was submitted in a modified form to 8 collaborating laboratories along with randomized and coded samples. Three laboratories used the manual method and 5 used the automated method. Other factors in the experimental design were ration types (broiler starter, broiler finisher, and pullet grower), feed form (meal vs. pellets), and potency level (90 and 110 g/ton) for one ration. Average recoveries for the ration types over all laboratories and feed forms were 87.7—93.13% of label, while mean recoveries in 2 feed forms were 91.7% for meal and 87.6% for pellets. Average recoveries in the 8 laboratories ranged from 84.6 to 106.64% of label for 90 g/ton rations and 87.1 to 106.6% for 110 g/ton rations. There was no significant difference between the manual and the automated methods. The collaborators’ assays were uniform with respect to within-laboratory variation. Relative standard deviations ranged from 4.51 to 10.76% with a median of 6.04%. Agreement with the plate assay is quite good. The turbidimetric method for monensin has been adopted as official first action.

Microbiological Assay of Monensin in Chicken Rations

1970 ◽  
Vol 53 (1) ◽  
pp. 49-53 ◽  
Author(s):  
R M Kline ◽  
R E Stricker ◽  
J D Coffman ◽  
H Bikin ◽  
R P Rathmacher
Keyword(s):  
Bacillus Subtilis ◽  
Statistical Analysis ◽  
Standard Deviation ◽  
Microbiological Assay ◽  
Assay Method ◽  
Microbiological Activity ◽  
Alumina Column ◽  
Poultry Feeds ◽  
Subtilis Atcc ◽  
Bacillus Subtilis Atcc

Abstract A microbiological assay method is described for the coccidiostat monensin in poultry feeds and prerhixes. Samples are extracted with methanol-water (9 + 1), and interfering substances are removed on an alumina column. Microbiological activity in the effluent is measured with Bacillus subtilis (ATCC 6633). Statistical analysis of data from several different rations prepared as meals and pellets indicate a mean recovery and standard deviation of 94.8 ± 9.7 g monensin/ton from a theoretical potency of 110 g monensin/ton. A mean recovery of 98 ± 7.6 µg/g was obtained from samples prepared by fortifying basal rations with t monensin at 110 µg/g Just before extraction.

A Microbiological Assay Method for Biotin

1951 ◽  
Vol 76 (2) ◽  
pp. 341-343 ◽  
Author(s):  
G. G. Villela ◽  
A. Cury
Keyword(s):  
Microbiological Assay ◽  
Assay Method

scholarly journals Comparison of inhibitory effect of ibuprofen with Piper guineense Schumach and Thonn. on some reproductive hormones in female rats

2017 ◽  
Vol 6 (4) ◽  
pp. 205-209
Author(s):  
Emmanuel Onuka Agbai ◽  
◽  
Chisomaga Chiwuikem Eke ◽  
Collins Okechukwu Nwanegwo ◽  
Ugochukwu Bond Anyaehie ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Inhibitory Effect ◽  
Female Rats ◽  
Assay Method ◽  
Piper Guineense ◽  
Serum Luteinizing Hormone ◽  
Group B ◽  
Group D ◽  
Stimulating Hormone

The aim of the present study was to compare the inhibitory effect of ibuprofen with oral administration of Piper guineense leaf extract on follicle-stimulating hormone, luteinizing hormone, progesterone and estrogen in female rats irrespective of the estrous cycle. The animals were randomly assigned to four groups (n = 7): group A (control), Group B, 180 mg-kg of ibuprofen, Group C, 200 mg-kg of Piper guineense extract, Group D, 180 mg-kg of ibuprofen and 200 mg-kg of Piper guineense extract. At the end of two weeks administration, rats were sacrificed under urethane anesthesia and hormones measured using enzyme-linked immunosorbent assay method. Results showed significant reduction in serum follicle-stimulating hormone and luteinizing hormone following ibuprofen administration in Group B rats at P < 0.05. Piper guineense extract treated Group C rats caused significant reduction in serum luteinizing hormone and progesterone at P < 0.05. In contrast, serum follicle stimulating hormone significantly increased in Group D rats at P < 0.05 whereas serum luteinizing hormone and progesterone were markedly reduced at P < 0.05. Serum estrogen level remained unchanged among groups. In conclusion, results obtained suggested that extract inhibited luteinization of follicles thus could impair ovulation, therefore the extract can be used as oral contraceptive in family planning.

A Rapid Microbiological Assay Method for Auxins, Gibberellic Acid and Kinetin Using Yeast

1974 ◽  
Vol 37 (1) ◽  
pp. 171-174 ◽  
Author(s):  
J. M. Barea ◽  
E. Navarro ◽  
A. Palomares ◽  
E. Montoya
Keyword(s):  
Gibberellic Acid ◽  
Microbiological Assay ◽  
Assay Method

scholarly journals A MICROBIOLOGICAL ASSAY METHOD FOR THIAMINE

1943 ◽  
Vol 150 (1) ◽  
pp. 1-9
Author(s):  
Charles F. Niven ◽  
Karl L. Smiley
Keyword(s):  
Microbiological Assay ◽  
Assay Method

scholarly journals In vitro equivalence of generic and branded amoxicillin tablet by microbiological assay method

2020 ◽  
Vol 30 (6) ◽  
Author(s):  
Primadi Avianto ◽  
Christopher Paul Alderman ◽  
◽  
◽  
Keyword(s):  
Generic Drugs ◽  
Healthcare Facility ◽  
Microbiological Assay ◽  
Assay Method ◽  
National Agency ◽  
Potency Ratio ◽  
Healthcare Facilities ◽  
E Coli ◽  
Inhibition Zones

AbstractBackgroundIndonesian Ministry of Health advocate doctors, especially in government-owned healthcare facility, to prescribe generic drugs including amoxicillin. Although BPOM (the National Agency of Drug and Food Control) already guarantees that the generic amoxicillin and the branded one were interchangeable, lack of confidence in generic drugs still remains among patients, pharmacists, and doctors. This issue supported by lack of publication confirmed the therapeutic equivalence of branded and generic drugs. This study aims to evaluate and compare the in vitro microbiological assay of different generic and branded amoxicillin that are available in Indonesian market, especially those used in government-owned healthcare facilities.MethodsMicrobiological assays for five samples of amoxicillin tablet containing 500 mg amoxicillin available in Indonesia were determined using a method from Indonesia Pharmacopeia. Samples were coded as Products A to E. The assay was carried out by measuring the diameter of the inhibition zones in the plate agar incubated with Escherichia coli and Staphylococcus aureus. The obtained data were evaluated to determine the sample potency and compared with the amoxicillin reference standard.ResultsMinor and insignificant differences (p > 0.05) were found in the diameters of the inhibition zones. Potency ratio measured both in E. coli and S. aureus were all between 95% and 105%. The lowest of the tested samples were from Product C, which resulted to ratio potencies of 96.3% and 95.5% in E. coli and S. aureus, respectively.ConclusionsAll five samples were in the range of the acceptance criteria. Therefore, from the view of the microbiological assay, these products are in equivalence in quality and are interchangeable.

Penicillin tolerance in Neisseria gonorrhoeae: evidence disallowing a penicillinase-mediated mechanism from a refined microbiological assay method

1976 ◽  
Vol 22 (1) ◽  
pp. 76-82 ◽  
Author(s):  
R. A. Scudamore ◽  
M. Goldner
Keyword(s):  
Escherichia Coli ◽  
Neisseria Gonorrhoeae ◽  
Dry Weight ◽  
Microbiological Assay ◽  
Assay Method ◽  
Benzyl Penicillin ◽  
Quantitative Evidence ◽  
Quantitative Manner ◽  
K 12

A microbiological assay method has been developed and applied to Neisseria gonorrhoeae, for the purpose of detecting enzymatic deactivation of benzyl penicillin. Calibration of the method, using strains of Escherichia coli K-12 with previously reported penicillinase (EC 3.5.2.6.) activities, has shown that it is extremely sensitive and may be used in a quantitative manner. At the limit of sensitivity the test is able to detect penicillin breakdown in the order of 3 × 10−3 μg in 48 h, which is equivalent to about 7 × 10−8 μmol/min per milligram dry weight of cells. Over 100 strains of N. gonorrhoeae, most of them resistant to penicillin, were screened for their ability to deactivate penicillin during 48 h of growth in the presence of subinhibitory levels. No deactivation was detected. It is concluded, from quantitative evidence, that reduced penicillin sensitivity in N. gonorrhoeae is not due to the enzymatic deactivation of the antibiotic.

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