In vitro germination of pollen grains of three native species from Pampa biome with ornamental potential

The aim of this work was to verify the in vitro germination of pollen grains of Angelonia integerrima L., Campomanesia aurea O. Berg and Sesbania punicea (Cav.) Benth in different culture medium and temperatures. For this purpose, flower buds from which pollen was collected and sprayed on plates containing the three evaluated culture medium: M1 - agar and sucrose; M2 - agar, sucrose and H3BO3; M3 - agar, sucrose, H3BO3, Ca(NO3), MgSO4 and KNO3; and two incubation temperatures (20 °C and 30 °C). Data was subjected to analysis of variance after its transformation to square root and means were compared by Fisher’s test (LSD). For the three species, the temperature of 30 ºC provided the highest percentage of pollen grain germination. For A. integerrima, M1 and M3 promoted the highest germination percentages (40.7 % and 56.5 %, respectively). On the other hand, for C. aurea, M2 provided the highest germination average (43.7 %). At last for S. punicea, M3 was the one that provided the highest average (31.62 %). It was concluded that the evaluated species differ in micronutrient requirements for in vitro germination of pollen grains. The temperature of 30 °C was suitable for all three species.

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In vitro pollen germination and pollen viability in passion fruit (Passiflora spp.)

Revista Brasileira de Fruticultura ◽

10.1590/s0100-29452013000400023 ◽

2013 ◽

Vol 35 (4) ◽

pp. 1116-1126 ◽

Cited By ~ 7

Author(s):

Taliane Leila Soares ◽

Onildo Nunes de Jesus ◽

Janay Almeida dos Santos-Serejo ◽

Eder Jorge de Oliveira

Keyword(s):

Pollen Germination ◽

Culture Medium ◽

Pollen Viability ◽

Pollen Grains ◽

In Vitro Germination ◽

Triphenyltetrazolium Chloride ◽

Histochemical Analysis ◽

High Viability ◽

Three Stages

The use of Passiflora species for ornamental purposes has been recently developed, but little is known about pollen viability and the potential for crossing different species. The objective of this study was to evaluate the pollen viability of six Passiflora species collected from different physiological stages of development through in vitro germination and histochemical analysis using dyes. The pollen was collected in three stages (pre-anthesis, anthesis and post-anthesis). Three compositions of culture medium were used to evaluate the in vitro germination, and two dyes (2,3,5-triphenyltetrazolium chloride, or TTC, and Lugol's solution) were used for the histochemical analysis. The culture medium containing 0.03% Ca(NO3) 4H2O, 0.02% of Mg(SO4 ).7H2O, 0.01% of KNO3, 0,01% of H3BO3, 15% sucrose, and 0.8% agar, pH 7.0, showed a higher percentage of pollen grains germinated. Anthesis is the best time to collect pollen because it promotes high viability and germination. The Lugol's solution and TTC dye overestimated the viability of pollen, as all accessions showed high viability indices when compared with the results obtained in vitro.

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Optimization of culture medium for the in vitro germination and histochemical analysis of Passiflora spp. pollen grains

Scientia Horticulturae ◽

10.1016/j.scienta.2021.110298 ◽

2021 ◽

Vol 288 ◽

pp. 110298

Author(s):

Michele dos Santos Ferreira ◽

Taliane Leila Soares ◽

Eva Maria Rodrigues Costa ◽

Ronilze Leite da Silva ◽

Onildo Nunes de Jesus ◽

...

Keyword(s):

Culture Medium ◽

Pollen Grains ◽

In Vitro Germination ◽

Histochemical Analysis

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Determination of cardinal temperatures for sugar apple ( Annona squamosa L.)

Ciência e Agrotecnologia ◽

10.1590/1413-70542016402039115 ◽

2016 ◽

Vol 40 (2) ◽

pp. 145-154 ◽

Cited By ~ 5

Author(s):

Bruno Rafael Alves Rodrigues ◽

Rayane Carneiro dos Santos ◽

Silvia Nietsche ◽

Maria Olívia Mercadante-Simões ◽

Isabella Renata Gomes da Cunha ◽

...

Keyword(s):

Pollen Tube ◽

Pollen Tube Growth ◽

Pollen Grains ◽

Pollen Grain ◽

Tube Growth ◽

Effect Of Temperature ◽

In Vitro Germination ◽

Cardinal Temperatures ◽

Germination Of Pollen

ABSTRACT The sugar apple is highly dependent on the pollination and fruit tree pollen performance is crucial for successful fertilization and fruit set. The objective of this study was to determine the cardinal temperatures for in vitro pollen grains germination and evaluate the effect of temperature on pollen tube growth of sugar apple. The experimental design was in a completely randomized with eight treatments (10, 15, 20, 25, 30, 35, 40 and 45 ºC), four replicates and each plot was constituted by two slides per parcel. The cardinal temperatures were determined by evaluating in vitro germination of pollen grains and pollen tube growth in standard culture medium. It also assessed the pollen tube growth and the percentage of germination in vitro depending on the type of pollen grain arrangement (monad, dyad, triad and tetrad). In vitro germination of pollen grains and pollen tube growth varied significantly with temperature. The maximum germination recorded (48.13%) and the maximum lengths of pollen tubes (536.45 μm) were obtained when pollen grains were cultivated at 25 ºC. The estimated cardinal temperatures were 9.7, 26.9 and 44.2 ºC. Among the pollen grain arrangements, tetrads pollen grains were observed in higher proportions, however, monads pollen grains presented higher germination percentage.

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In Vitro Germination of Pollen Grains in Pitahaya Species

International Journal of Fruit Science ◽

10.1080/15538362.2021.1913469 ◽

2021 ◽

Vol 21 (1) ◽

pp. 556-564

Author(s):

Miriã Cristina Pereira Fagundes ◽

José Darlan Ramos ◽

Nathália Vallery Tostes ◽

Moacir Pasqual ◽

Filipe Almendagna Rodrigues

Keyword(s):

Pollen Grains ◽

In Vitro Germination ◽

Germination Of Pollen

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IN VITRO GERMINATION OF POLLEN GRAINS FOR PRUNUS PERSICA (L.) BATSCH NUCIPERSICA

Acta Horticulturae ◽

10.17660/actahortic.2012.962.37 ◽

2012 ◽

pp. 261-267 ◽

Cited By ~ 1

Author(s):

R. Pio ◽

E.A. Chagas ◽

W. Barbosa ◽

P.C. Chagas ◽

J.E. Bettiol Neto ◽

...

Keyword(s):

Prunus Persica ◽

Pollen Grains ◽

In Vitro Germination ◽

Germination Of Pollen

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Morphological aspects of fruits, seeds, seedlings and in vivo and in vitro germination of species of the genus Cleome

Journal of Seed Science ◽

10.1590/2317-1545v36n31013 ◽

2014 ◽

Vol 36 (3) ◽

pp. 326-335 ◽

Cited By ~ 1

Author(s):

Tatiana Carvalho de Castro ◽

Claudia Simões-Gurgel ◽

Ivan Gonçalves Ribeiro ◽

Marsen Garcia Pinto Coelho ◽

Norma Albarello

Keyword(s):

Urban Areas ◽

Native Species ◽

Human Impacts ◽

In Vitro Germination ◽

Morphological Aspects ◽

Physiological Dormancy ◽

Cleome Spinosa ◽

The Tropics

The genus Cleome is widely distributed in drier areas of the tropics and subtropics. Cleome dendroides and C. rosea are Brazilian native species that occur mainly in Atlantic Forest and sandy coastal plains, respectively ecosystems negatively affected by human impacts. Cleome spinosa is frequently found in urban areas. Many Cleome species have been used in traditional medicine, as C. spinosa. In the present work, was investigated C. dendroides, C. rosea and C. spinosa germinative behavior under in vivo conditions, as well as was established suitable conditions to in vitro germination and seedling development. The in vivo germination was performed evaluating the influence of temperature, substrate and light. It was observed that only C. spinosa seeds presents physiological dormancy, which was overcome by using alternate temperatures. The substrate influenced significantly the germination of C. rosea and the seeds of C. dendroides showed the highest germination percentages in the different conditions evaluated. The post-seminal development stages under in vivo and in vitro conditions were defined. It was observed that the development was faster under in vitro than in vivo conditions. An effective methodology for in vitro germination, enabling the providing of material to experiment on plant tissue culture was established to C. dendroides and C. spinosa.

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ROS in tobacco stigma exudate affect pollen proteome and provoke membrane hyperpolarization

10.1101/2020.04.28.065706 ◽

2020 ◽

Author(s):

Breygina Maria ◽

Klimenko Ekaterina ◽

Shilov Eugeny ◽

Mamaeva Anna ◽

Zgoda Viktor ◽

...

Keyword(s):

Cell Metabolism ◽

Pollen Grains ◽

Plant Reproduction ◽

List Type ◽

Membrane Hyperpolarization ◽

Inhibitory Analysis ◽

The One ◽

Cellular Components ◽

Stigma Exudate

1.AbstractROS are known to be accumulated in stigmas of different species and can possibly perform different functions in plant reproduction. Here we confirm the assumption that they affect pollen by altering ion transport through the plasma membrane; as a more deferred effect, pollen proteome is modified. We detected ROS in stigma exudate, found hyperpolarization in exudate-treated growing pollen tubes and used flow cytometry of pollen protoplasts to compare the effects of fresh exudate and exogenous H2O2 on pollen tube plasmalemma. Exudate causes plasmalemma hyperpolarization similar to the one provoked by H2O2, which is abolished by catalase treatment and ROS quencher MnTMPP. Inhibitory analysis indicates the participation of Ca2+- and K+-conducting channels in the observed hyperpolarization, linking obtained data with previous patch-clamp studies in vitro. For a deeper understanding of pollen response to ROS we analyzed proteome alterations in H2O2-treated pollen grains. We found 50 unique proteins and 20 differently accumulated proteins that are mainly involved in cell metabolism, energetics, protein synthesis and folding. Thus, pollen is getting ready for effective resource usage, construction of cellular components and rapid growth.HighlightsThe active substance in stigma exudate is H2O2H2O2 causes hyperpolarization mediated by the activation of cation channels.H2O2 affects pollen proteome; we found 50 unique proteins.

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ACTIVATED CHARCOAL APPLICATION FOR THE MICROPROPAGATION OF Cattleya crispata (Thunb.) Van den Berg

Nativa ◽

10.31413/nativa.v9i4.12164 ◽

2021 ◽

Vol 9 (4) ◽

pp. 352-358

Author(s):

Denys Matheus Santana Costa Souza ◽

Sérgio Bruno Fernandes ◽

Letícia Vaz Molinari ◽

Maria Lopes Martins Avelar ◽

Gilvano Ebling Brondani

Keyword(s):

Endemic Species ◽

Activated Charcoal ◽

Culture Medium ◽

Genetic Conservation ◽

Campo Rupestre ◽

In Vitro Germination ◽

Germination Speed

Micropropagation is an alternative for the genetic conservation and propagation of endemic species from “Campo Rupestre Ferruginoso”, such as the orchid Cattleya crispata. The aim of the present study is to assess the influence of activated charcoal on the in vitro germination, multiplication and elongation phases of C. crispata. Seeds extracted from mature capsules were used for inoculation in the culture medium that was adopted to assess the effect of supplementation, or not, with activated charcoal. Data about germination speed, seedling number, length, vigor, oxidation and contamination (bacterial and/or fungal) were assessed through these phases. Based on the results obtained, the use of activated charcoal was efficient in the in vitro germination and multiplication phases of C. crispata, providing greater speed and percentage of germination, less contamination and oxidation of the tissues, greater number, length and vigor of shoots, being effective for the genetic conservation and production of seedlings of the species. Culture medium without the supplementation of activated charcoal provided the best results for the in vitro elongation, with greater length, vigor and less oxidation of shoots.

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