VIABILITY AND IN VITRO GERMINATION RATE OF POLLEN GRAINS OF D. KAKI THUNB., D. LOTUS L. AND D. VIRGINIANA L. IN RELATION TO STORAGE TIME AND TEMPERATURES

2013 ◽  
pp. 97-102 ◽  
Author(s):  
A. Ferri ◽  
E. Giordani ◽  
C. Benelli
Keyword(s):  
Storage Time ◽  
Germination Rate ◽  
Pollen Grains ◽  
In Vitro Germination

scholarly journals Floral Biology and in vitro Germination of Nasturtium Pollen Grains Grown With Poultry Litter

2019 ◽  
Vol 11 (7) ◽  
pp. 160
Author(s):  
G. T. Zanetti ◽  
L. M. C. Davide ◽  
M. do C. Vieira ◽  
N. A. H. Zárate ◽  
A. M. T. Alovisi ◽  
...  
Keyword(s):  
Culture Media ◽  
Germination Rate ◽  
Poultry Litter ◽  
Pollen Grains ◽  
Floral Biology ◽  
Basic Knowledge ◽  
In Vitro Germination ◽  
Tropaeolum Majus ◽  
Floral Bud

The plant Tropaeolum majus, popularly known as nasturtium, presents food, ornamental and medicinal importance. Studies on floral and carpometric characteristics generate basic knowledge for areas of science such as botany, genetics and plant breeding. This work has the objectives of analyzing the effect of combinations of doses of poultry litter on floral structures and the in vitro germination of T. majus pollen grains. From the experimental matrix Plan Puebla III were defined 9 combinations of poultry litter doses incorporated and covered. The design of the experiments was entirely random, varying the replicates number. The number of stamens, petals, sepals and carpels was verified per floral bud in each treatment. The in vitro germination rate of pollen grains was estimated in 5 culture media and 2 germination times, from flower buds randomly collected in the experiment. The overall mean of stamens, sepals, petals and carpels per floral bud was 8.23; 5; 5 and 3, respectively. The combinations of poultry litter doses influenced only the number of stamens. The in vitro germination rate of T. majus pollen grains varied in the culture media. There was no association between the combinations of poultry litter doses and the in vitro germination rate of pollen grains.

scholarly journals The influence of light spectra, UV-A, and growth regulators on the in vitro seed germination of Senecio cineraria DC.

Revista CERES ◽  
2010 ◽  
Vol 57 (5) ◽  
pp. 576-580 ◽  
Author(s):  
Cristiane Pimentel Victório ◽  
Nina Cláudia Barbosa da Silva ◽  
Maria Apparecida Esquibel ◽  
Alice Sato
Keyword(s):  
Seed Germination ◽  
Growth Regulators ◽  
White Light ◽  
Germination Rate ◽  
Germination Percentage ◽  
Ms Medium ◽  
In Vitro Germination ◽  
Light Spectra ◽  
Indole 3 Acetic Acid

This study was carried out to investigate the effects of light spectra, additional UV-A, and different growth regulators on the in vitro germination of Senecio cineraria DC. Seeds were surface-sterilized and inoculated in MS medium to evaluate the following light spectra: white, white plus UV-A, blue, green, red or darkness. The maximum germinability was obtained using MS0 medium under white light (30%) and MS + 0.3 mg L-1 GA3 in the absence of light (30.5%). S. cineraria seeds were indifferent to light. Blue and green lights inhibited germination. Different concentrations of gibberellic acid (GA3) (0.1; 0.4; 0.6; 0.8; 1.0 and 2.0 mg L-1) and indole-3-acetic acid IAA (0.1; 0.3 and 1.0 mg L-1) were evaluated under white light and darkness. No concentration of GA3 enhanced seed germination percentage under white light. However, when the seeds were maintained in darkness, GA3 improved germination responses in all tested concentrations, except at 1.0 mg L-1. Under white light, these concentrations also increased the germination time and reduced germination rate. Germination rate, under light or darkness, was lower using IAA compared with GA3.

scholarly journals Responses of Creeping Bentgrass to Salt Stress during In Vitro Germination

HortScience ◽  
2010 ◽  
Vol 45 (11) ◽  
pp. 1747-1750 ◽  
Author(s):  
Sheng Wang ◽  
Qi Zhang
Keyword(s):  
Germination Rate ◽  
Creeping Bentgrass ◽  
Fluorescent Light ◽  
In Vitro Germination ◽  
Salt Tolerant ◽  
Recycled Water ◽  
Salt Selection ◽  
Salinity Levels ◽  
Salt Tolerant Cultivars

Many golf courses and turfgrass managers use recycled water, which contains high salts, as part or a sole irrigation source to lower costs and comply with governmental restrictions on water use. High salinity negatively affects turfgrass performance. Using salt-tolerant species or cultivars is one the most effective methods to address salinity problems. Twenty-six commercially available creeping bentgrass (Agrostis stolonifera) cultivars were evaluated for salt tolerance during in vitro germination on 1% agar media supplemented with NaCl at 0, 5, 10, 15, or 20 g·L−1 at 25/15 °C (day/night) under fluorescent light (36 μmol·s−1·m−2) with an 8- to16-h photoperiod. Significant variations in salinity tolerance were observed among the cultivars. Final germination rate (FGR, %) and daily germination rate (DGR, %/d) decreased linearly or quadratically as salinity levels increased. ‘Declaration’, ‘Seaside II’, ‘T-1’, and ‘Bengal’ were the most salt-tolerant, requiring salt levels at or greater than 16.0 and 10.0 g·L−1, respectively, to reduce FGR and DGR by 50%. In contrast, ‘Tyee’, ‘Kingpin’, and ‘SR1150’ required average salinity levels of 11.6 and 6.5 g·L−1 to cause 50% reduction in FGR and DGR, respectively, showing that they were the least salt-tolerant cultivars. The largest difference between FGR (1.9%) and DGR (26.2%) reduction under saline conditions was observed at 5 g·L−1, indicating that DGR was more sensitive to salinity changes than FGR. Therefore, DGR might be a more reliable method to be used for salt selection.

scholarly journals Anatomical Characteristics of Petalized Anther Abortion in Male Sterile Camellia oleifera Plants

2021 ◽  
pp. 1-13
Author(s):  
Yang Hu ◽  
Chao Gao ◽  
Quanen Deng ◽  
Jie Qiu ◽  
Hongli Wei ◽  
...  
Keyword(s):  
Germination Rate ◽  
Pollen Grains ◽  
Relative Activity ◽  
Control Group ◽  
Camellia Oleifera ◽  
Male Sterile ◽  
Flower Bud ◽  
Triphenyltetrazolium Chloride ◽  
Pollen Sacs

Petalized anther abortion is an important characteristic of male sterility in plants. The male sterile plants (HB-21) evincing petalized anther abortion previously discovered in a clone population of the Camellia oleifera cultivar Huashuo by our research group were selected as the experimental material in this study. Using plant microscopy and anatomic methods and given the correspondence between external morphology and internal structure, we studied the anatomic characteristics of petalized anther abortion (with a fertile plant as the control group) in various stages, from flower bud differentiation to anther maturity, in hopes of providing a theoretical basis for research on and applications of male sterile C. oleifera plants, a new method for the selection of male sterile C. oleifera cultivars, and improvements in the yield and quality of C. oleifera. In this study, the development of anthers in C. oleifera was divided into 14 stages. Petalized anther abortion in male sterile plants was mainly initiated in the second stage (the stage of sporogenous cells). Either the petalized upper anther parts did not form pollen sacs, or the entire anthers did not form pollen sacs. The lower parts of some anthers could form deformed pollen sacs and develop, and these anthers could be roughly divided into two types: fully and partially petalized anthers. Abnormal callose and the premature degradation of the tapetum occurred in the pollen sacs formed by partially petalized anthers during the development process, resulting in the absence of inclusions in the pollen grains formed. Small quantities of mature pollen grains withered inward from the germinal furrows, exhibiting obvious abortion characteristics. The relative in vitro germination rate of the pollen produced by the partially petalized anthers of sterile plants was 11.20%, and the relative activity of triphenyltetrazolium chloride was 3.24%, while the fully petalized anthers did not generate pollen grains. Either the petalized anthers in male sterile plants did not produce pollen, or the vitality of the small amounts of pollen produced by sterile plants was very low compared with that of fertile plants. Such male sterile plants could be used to select correct clones and have good prospects for application in production.

scholarly journals Pecan Pollen Stored Over a Decade Retains Viability

HortScience ◽  
2002 ◽  
Vol 37 (1) ◽  
pp. 176-177 ◽  
Author(s):  
Darrell Sparks ◽  
I.E. Yates
Keyword(s):  
Liquid Nitrogen ◽  
Pollen Morphology ◽  
Germ Tube ◽  
Pollen Grains ◽  
Carya Illinoinensis ◽  
In Vitro Germination ◽  
Fresh Pollen

In vitro germination of freshly collected pollen and pollen stored 1, 10, 11, 12, and 13 years in liquid nitrogen was examined for `Desirable' pecan [Carya illinoinensis (Wangenh.) C. Koch]. Viability of pollen stored in liquid nitrogen for 1, 10, 11, 12, and 13 years was not diminished in comparison to that of fresh pollen. Morphology of stored pollen grains and the germ tube was normal. Thus, liquid nitrogen may offer a means of haploid preservation of pecan.

scholarly journals Optimization of In Vitro Pecan Pollen Germination

HortScience ◽  
2011 ◽  
Vol 46 (4) ◽  
pp. 571-576 ◽  
Author(s):  
Patrick J. Conner
Keyword(s):  
Polyethylene Glycol ◽  
Pollen Germination ◽  
Pollen Grains ◽  
Solid Support ◽  
Carya Illinoinensis ◽  
Testing System ◽  
In Vitro Germination ◽  
Ethanesulfonic Acid ◽  
Made In

Storage of pollen from 1 year to the next is often needed to enable desired crosses to be made in a pecan [Carya illinoinensis (Wangenh.) K. Koch] breeding program. Stored pollen is usually tested for viability through the use of in vitro germination tests. An in vitro germination testing system was developed for this purpose using cellophane booklets to provide a solid support for the pollen grains. Optimized germination media contained 5% sucrose, 20% polyethylene glycol 8000, 0.05% Ca(NO3)2, 0.025% H3BO3, and 10 mm 2-(N-morpholino)ethanesulfonic acid pH 6.0. Pollen should be rehydrated for 2 to 4 h in a humidified chamber before germination testing. A germination time of 4 to 24 h produces similar final germination percentages. Testing of pollen samples stored at –80 °C indicates that pecan pollen can be stored for at least 8 years without a decrease in viability. Chemical names used: polyethylene glycol (PEG); 2-(N-morpholino)ethanesulfonic acid (MES).

scholarly journals Viability of pollen grains and stigma receptivity in Desert Rose

2022 ◽  
Vol 28 (1) ◽  
pp. 92-98
Author(s):  
Cristiane Gonçalves Souza ◽  
Sabrina Maihave Barbosa Ramos ◽  
Silvia Nietsche ◽  
Clivia Carolina Fiorilo Possobom ◽  
Elka Fabiana Aparecida Almeida ◽  
...  
Keyword(s):  
Abiotic Factors ◽  
Pollen Viability ◽  
Oxygen Demand ◽  
Pollen Grains ◽  
Floral Biology ◽  
Effect Of Temperature ◽  
In Vitro Germination ◽  
Stigma Receptivity ◽  
Flower Opening

Abstract Adenium obesum (Forssk.) Roem. & Schult., popularly known as desert rose, has become a valuable ornamental plant. In floriculture, the production of hybrids is prioritized. Hence, knowledge on floral biology and sexual reproduction of the target species is fundamental. The objectives of this study were: (1) to test sucrose concentrations and temperatures for in vitro germination of A. obesum pollen grains; (2) to identify the effect of temperature on the viability of A. obesum pollen grains; and (3) to evaluate the viability of pollen grains and stigma receptivity in pre-anthesis, at flower opening, and 72 h post-flower opening in three accessions of A. obesum. A significant relationship between temperatures and sucrose concentrations was observed in the in vitro germination test. The highest percentage of in vitro germination of pollen grains, 39.81%, was observed at an estimated temperature of 26.05 °C. Desert rose accessions maintained in biochemical oxygen demand (BOD) chambers at 30 °C during a 16-h light photoperiod showed faster flowering, and temperatures ≥ 25 °C induced pollen grain viability percentages above 69%. Temperature is one of the most important abiotic factors, influencing mainly in pollen germination, pollen tube growing and in efficiency fertilization. The ICA-wd accession stood out and can be considered a pollen donor in artificial pollination. The stigmas of flowers were receptive from a day before flower opening until three days after. The two parameters presented above, stigma receptivity and pollen viability, allow inferences about the appropriate time for successful pollination and subsequent fertilization in desert roses.

scholarly journals In vitro seed germination and multiplication of Calophyllum brasiliense

2016 ◽  
Vol 36 (87) ◽  
pp. 185
Author(s):  
Sheila Susy Silveira ◽  
Juliana Degenhardt-Goldbach ◽  
Marguerite Germaine Ghislaine Quoirin
Keyword(s):  
Bacterial Contamination ◽  
Culture Medium ◽  
Germination Rate ◽  
Nodal Segment ◽  
In Vitro Germination ◽  
Fungal Contamination ◽  
Natural Reproduction ◽  
Calophyllum Brasiliense ◽  
Free Rate

Calophyllum brasiliense is a tree species with limited natural reproduction. In vitro germination may be an alternative for obtaining high-quality seedlings. Seeds were maintained in water before surface disinfestation and compared with control seeds (i.e. not immersed), without differences between treatments. HgCl2 used during surface-disinfestation reduced contamination rates of cultures. Fungal contamination was reduced with fungicide added to culture medium (23 to 6.4%), although bacterial contamination increased (24 to 36%). In another experiment, seeds were immersed in plant preservative mixture (PPM™) prior to surface disinfestation. By combining immersion for 48 h and 2 mL L-1 in culture medium, contamination was only 6%. Seeds immersion in GA3 prior to surface disinfestation reduced root formation as concentration increased. Germination rate and GSI were reduced, respectively, from 72% and 0.129 (24 h) to 60% and 0.092 (48 h) according to exposure time to GA3. After 90 days in multiplication medium containing benzylaminopurine, average number of shoots per nodal segment was 3.4. In conclusion, in vitro germination of C. brasiliense seeds is feasible in sucrose-free WPM medium and reaches a high contamination-free rate (up to 93.3%).

scholarly journals In vitro germination and viability of pea pollen grains after application of organic nano-fertilizers

2017 ◽  
Vol 32 (1) ◽  
pp. 61-65 ◽  
Author(s):  
Natalia Georgieva ◽  
Ivelina Nikolova ◽  
Valentin Kosev ◽  
Yordanka Naydenova
Keyword(s):  
Pollen Tube ◽  
Pollen Germination ◽  
Culture Media ◽  
Pollen Viability ◽  
Pollen Grains ◽  
Pollen Grain ◽  
In Vitro Germination ◽  
Pisum Sativum L ◽  
Pollen Tube Elongation

The objective of this study was to evaluate the influence of two organic nanofertilizers, Lithovit and Nagro, on in vitro germination, pollen tube elongation and pollen grain viability of Pisum sativum L cv. Pleven 4. The effect of their application was high and exceeded data for the untreated control (44.2 and 47.23 % regarding pollen germination and pollen tube elongation, respectively), as well as the effect of the control organic algal fertilizer Biofa (17.5 and 27.9 %, respectively). Pollen grains were inoculated in four culture media. A medium containing 15% sucrose and 1% agar had the most stimulating impact on pea pollen grains. Pollen viability, evaluated by staining with 1% carmine, was within limits of 74.72-87.97%. The highest viability of pollen grains was demonstrated after the application of Nagro organic nano-fertlizer.

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