Optimization of culture medium for the in vitro germination and histochemical analysis of Passiflora spp. pollen grains

2021 ◽  
Vol 288 ◽  
pp. 110298
Author(s):  
Michele dos Santos Ferreira ◽  
Taliane Leila Soares ◽  
Eva Maria Rodrigues Costa ◽  
Ronilze Leite da Silva ◽  
Onildo Nunes de Jesus ◽  
...  
Keyword(s):  
Culture Medium ◽  
Pollen Grains ◽  
In Vitro Germination ◽  
Histochemical Analysis

scholarly journals In vitro pollen germination and pollen viability in passion fruit (Passiflora spp.)

2013 ◽  
Vol 35 (4) ◽  
pp. 1116-1126 ◽  
Author(s):  
Taliane Leila Soares ◽  
Onildo Nunes de Jesus ◽  
Janay Almeida dos Santos-Serejo ◽  
Eder Jorge de Oliveira
Keyword(s):  
Pollen Germination ◽  
Culture Medium ◽  
Pollen Viability ◽  
Pollen Grains ◽  
In Vitro Germination ◽  
Triphenyltetrazolium Chloride ◽  
Histochemical Analysis ◽  
High Viability ◽  
Three Stages

The use of Passiflora species for ornamental purposes has been recently developed, but little is known about pollen viability and the potential for crossing different species. The objective of this study was to evaluate the pollen viability of six Passiflora species collected from different physiological stages of development through in vitro germination and histochemical analysis using dyes. The pollen was collected in three stages (pre-anthesis, anthesis and post-anthesis). Three compositions of culture medium were used to evaluate the in vitro germination, and two dyes (2,3,5-triphenyltetrazolium chloride, or TTC, and Lugol's solution) were used for the histochemical analysis. The culture medium containing 0.03% Ca(NO3) 4H2O, 0.02% of Mg(SO4 ).7H2O, 0.01% of KNO3, 0,01% of H3BO3, 15% sucrose, and 0.8% agar, pH 7.0, showed a higher percentage of pollen grains germinated. Anthesis is the best time to collect pollen because it promotes high viability and germination. The Lugol's solution and TTC dye overestimated the viability of pollen, as all accessions showed high viability indices when compared with the results obtained in vitro.

scholarly journals In vitro germination of pollen grains of three native species from Pampa biome with ornamental potential

2020 ◽  
Vol 11 ◽  
pp. e3217
Author(s):  
Marília Tedesco ◽  
Luciano da Silva Alves ◽  
Eduarda Demari Avrella ◽  
Carine Simioni ◽  
Gilmar Schafer
Keyword(s):  
Native Species ◽  
Culture Medium ◽  
Pollen Grains ◽  
In Vitro Germination ◽  
Flower Buds ◽  
Pampa Biome ◽  
The One ◽  
Incubation Temperatures ◽  
Germination Of Pollen

The aim of this work was to verify the in vitro germination of pollen grains of Angelonia integerrima L., Campomanesia aurea O. Berg and Sesbania punicea (Cav.) Benth in different culture medium and temperatures. For this purpose, flower buds from which pollen was collected and sprayed on plates containing the three evaluated culture medium: M1 - agar and sucrose; M2 - agar, sucrose and H3BO3; M3 - agar, sucrose, H3BO3, Ca(NO3), MgSO4 and KNO3; and two incubation temperatures (20 °C and 30 °C). Data was subjected to analysis of variance after its transformation to square root and means were compared by Fisher’s test (LSD). For the three species, the temperature of 30 ºC provided the highest percentage of pollen grain germination. For A. integerrima, M1 and M3 promoted the highest germination percentages (40.7 % and 56.5 %, respectively). On the other hand, for C. aurea, M2 provided the highest germination average (43.7 %). At last for S. punicea, M3 was the one that provided the highest average (31.62 %). It was concluded that the evaluated species differ in micronutrient requirements for in vitro germination of pollen grains. The temperature of 30 °C was suitable for all three species.

scholarly journals ACTIVATED CHARCOAL APPLICATION FOR THE MICROPROPAGATION OF Cattleya crispata (Thunb.) Van den Berg

Nativa ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 352-358
Author(s):  
Denys Matheus Santana Costa Souza ◽  
Sérgio Bruno Fernandes ◽  
Letícia Vaz Molinari ◽  
Maria Lopes Martins Avelar ◽  
Gilvano Ebling Brondani
Keyword(s):  
Endemic Species ◽  
Activated Charcoal ◽  
Culture Medium ◽  
Genetic Conservation ◽  
Campo Rupestre ◽  
In Vitro Germination ◽  
Germination Speed

 Micropropagation is an alternative for the genetic conservation and propagation of endemic species from “Campo Rupestre Ferruginoso”, such as the orchid Cattleya crispata. The aim of the present study is to assess the influence of activated charcoal on the in vitro germination, multiplication and elongation phases of C. crispata. Seeds extracted from mature capsules were used for inoculation in the culture medium that was adopted to assess the effect of supplementation, or not, with activated charcoal. Data about germination speed, seedling number, length, vigor, oxidation and contamination (bacterial and/or fungal) were assessed through these phases. Based on the results obtained, the use of activated charcoal was efficient in the in vitro germination and multiplication phases of C. crispata, providing greater speed and percentage of germination, less contamination and oxidation of the tissues, greater number, length and vigor of shoots, being effective for the genetic conservation and production of seedlings of the species. Culture medium without the supplementation of activated charcoal provided the best results for the in vitro elongation, with greater length, vigor and less oxidation of shoots.

scholarly journals Pecan Pollen Stored Over a Decade Retains Viability

HortScience ◽  
2002 ◽  
Vol 37 (1) ◽  
pp. 176-177 ◽  
Author(s):  
Darrell Sparks ◽  
I.E. Yates
Keyword(s):  
Liquid Nitrogen ◽  
Pollen Morphology ◽  
Germ Tube ◽  
Pollen Grains ◽  
Carya Illinoinensis ◽  
In Vitro Germination ◽  
Fresh Pollen

In vitro germination of freshly collected pollen and pollen stored 1, 10, 11, 12, and 13 years in liquid nitrogen was examined for `Desirable' pecan [Carya illinoinensis (Wangenh.) C. Koch]. Viability of pollen stored in liquid nitrogen for 1, 10, 11, 12, and 13 years was not diminished in comparison to that of fresh pollen. Morphology of stored pollen grains and the germ tube was normal. Thus, liquid nitrogen may offer a means of haploid preservation of pecan.

scholarly journals Optimization of In Vitro Pecan Pollen Germination

HortScience ◽  
2011 ◽  
Vol 46 (4) ◽  
pp. 571-576 ◽  
Author(s):  
Patrick J. Conner
Keyword(s):  
Polyethylene Glycol ◽  
Pollen Germination ◽  
Pollen Grains ◽  
Solid Support ◽  
Carya Illinoinensis ◽  
Testing System ◽  
In Vitro Germination ◽  
Ethanesulfonic Acid ◽  
Made In

Storage of pollen from 1 year to the next is often needed to enable desired crosses to be made in a pecan [Carya illinoinensis (Wangenh.) K. Koch] breeding program. Stored pollen is usually tested for viability through the use of in vitro germination tests. An in vitro germination testing system was developed for this purpose using cellophane booklets to provide a solid support for the pollen grains. Optimized germination media contained 5% sucrose, 20% polyethylene glycol 8000, 0.05% Ca(NO3)2, 0.025% H3BO3, and 10 mm 2-(N-morpholino)ethanesulfonic acid pH 6.0. Pollen should be rehydrated for 2 to 4 h in a humidified chamber before germination testing. A germination time of 4 to 24 h produces similar final germination percentages. Testing of pollen samples stored at –80 °C indicates that pecan pollen can be stored for at least 8 years without a decrease in viability. Chemical names used: polyethylene glycol (PEG); 2-(N-morpholino)ethanesulfonic acid (MES).

scholarly journals Viability of pollen grains and stigma receptivity in Desert Rose

2022 ◽  
Vol 28 (1) ◽  
pp. 92-98
Author(s):  
Cristiane Gonçalves Souza ◽  
Sabrina Maihave Barbosa Ramos ◽  
Silvia Nietsche ◽  
Clivia Carolina Fiorilo Possobom ◽  
Elka Fabiana Aparecida Almeida ◽  
...  
Keyword(s):  
Abiotic Factors ◽  
Pollen Viability ◽  
Oxygen Demand ◽  
Pollen Grains ◽  
Floral Biology ◽  
Effect Of Temperature ◽  
In Vitro Germination ◽  
Stigma Receptivity ◽  
Flower Opening

Abstract Adenium obesum (Forssk.) Roem. & Schult., popularly known as desert rose, has become a valuable ornamental plant. In floriculture, the production of hybrids is prioritized. Hence, knowledge on floral biology and sexual reproduction of the target species is fundamental. The objectives of this study were: (1) to test sucrose concentrations and temperatures for in vitro germination of A. obesum pollen grains; (2) to identify the effect of temperature on the viability of A. obesum pollen grains; and (3) to evaluate the viability of pollen grains and stigma receptivity in pre-anthesis, at flower opening, and 72 h post-flower opening in three accessions of A. obesum. A significant relationship between temperatures and sucrose concentrations was observed in the in vitro germination test. The highest percentage of in vitro germination of pollen grains, 39.81%, was observed at an estimated temperature of 26.05 °C. Desert rose accessions maintained in biochemical oxygen demand (BOD) chambers at 30 °C during a 16-h light photoperiod showed faster flowering, and temperatures ≥ 25 °C induced pollen grain viability percentages above 69%. Temperature is one of the most important abiotic factors, influencing mainly in pollen germination, pollen tube growing and in efficiency fertilization. The ICA-wd accession stood out and can be considered a pollen donor in artificial pollination. The stigmas of flowers were receptive from a day before flower opening until three days after. The two parameters presented above, stigma receptivity and pollen viability, allow inferences about the appropriate time for successful pollination and subsequent fertilization in desert roses.

scholarly journals In vitro germination, initial development and acclimatization of Cattleya nobilior Rchb. f. (Orchidaceae): an approach to curb the eventual endangerment of this exuberant, near-threatened Cerrado species

2021 ◽  
Vol 6 (2) ◽  
pp. 2167-2191
Author(s):  
Silene Lívia Aires de Oliveira ◽  
Kellen Lagares Ferreira Silva ◽  
Rafael José de Oliveira ◽  
Maíra Jéssica de Souza ◽  
Jaderson Roney Gomes de Oliveira ◽  
...  
Keyword(s):  
Large Scale ◽  
Culture Medium ◽  
Culture Media ◽  
Natural Environments ◽  
In Vitro Germination ◽  
Initial Development ◽  
Anatomical Characteristics ◽  
Soybean Straw ◽  
Leaf Anatomical Characteristics

ABSTRACT: The objective of the present study was to evaluate the effects of different culture media on the in vitro germination and initial development of Cattleya nobilior, and to acclimatize this species using soybean straw as a substrate component, aiming the production of plants at large scale to reintroduce it in natural environments. Anatomical characteristics associated with development during acclimatization are also depicted. The influence of Murashige and Skoog, Knudson, and Vacin and Went culture media on the in vitro germination and protocorm development were assessed. Acclimatization was accomplished using different proportions of soybean straw (SS) and Bioplant (BP) as substrates. Anatomical studies were conducted on the leaves of plants during acclimatization. KC was the most suitable culture medium for both germination and initial development of C. nobilior. Regarding acclimatization, it is recommended that a substrate composed of 60% SR and 40% BP be initially used. Upon transfer to shade-house conditions, plants should be grown in a mix of 40% SR: and 60% BP. The leaf anatomical characteristics observed are typical of epiphytic orchids adapted to water-poor environments, which indicates that SR did not negatively affect the development of the species. The in vitro propagation of C. nobilior as described herein is efficient for its multiplication for commercial and conservation purposes and SS can be used as an alternative component of the substrate for its acclimatization. KEYWORDS: Foliar anatomy, culture media, soybean straw.

scholarly journals In vitro seed germination and multiplication of Calophyllum brasiliense

2016 ◽  
Vol 36 (87) ◽  
pp. 185
Author(s):  
Sheila Susy Silveira ◽  
Juliana Degenhardt-Goldbach ◽  
Marguerite Germaine Ghislaine Quoirin
Keyword(s):  
Bacterial Contamination ◽  
Culture Medium ◽  
Germination Rate ◽  
Nodal Segment ◽  
In Vitro Germination ◽  
Fungal Contamination ◽  
Natural Reproduction ◽  
Calophyllum Brasiliense ◽  
Free Rate

Calophyllum brasiliense is a tree species with limited natural reproduction. In vitro germination may be an alternative for obtaining high-quality seedlings. Seeds were maintained in water before surface disinfestation and compared with control seeds (i.e. not immersed), without differences between treatments. HgCl2 used during surface-disinfestation reduced contamination rates of cultures. Fungal contamination was reduced with fungicide added to culture medium (23 to 6.4%), although bacterial contamination increased (24 to 36%). In another experiment, seeds were immersed in plant preservative mixture (PPM™) prior to surface disinfestation. By combining immersion for 48 h and 2 mL L-1 in culture medium, contamination was only 6%. Seeds immersion in GA3 prior to surface disinfestation reduced root formation as concentration increased. Germination rate and GSI were reduced, respectively, from 72% and 0.129 (24 h) to 60% and 0.092 (48 h) according to exposure time to GA3. After 90 days in multiplication medium containing benzylaminopurine, average number of shoots per nodal segment was 3.4. In conclusion, in vitro germination of C. brasiliense seeds is feasible in sucrose-free WPM medium and reaches a high contamination-free rate (up to 93.3%).

scholarly journals In vitro germination and viability of pea pollen grains after application of organic nano-fertilizers

2017 ◽  
Vol 32 (1) ◽  
pp. 61-65 ◽  
Author(s):  
Natalia Georgieva ◽  
Ivelina Nikolova ◽  
Valentin Kosev ◽  
Yordanka Naydenova
Keyword(s):  
Pollen Tube ◽  
Pollen Germination ◽  
Culture Media ◽  
Pollen Viability ◽  
Pollen Grains ◽  
Pollen Grain ◽  
In Vitro Germination ◽  
Pisum Sativum L ◽  
Pollen Tube Elongation

The objective of this study was to evaluate the influence of two organic nanofertilizers, Lithovit and Nagro, on in vitro germination, pollen tube elongation and pollen grain viability of Pisum sativum L cv. Pleven 4. The effect of their application was high and exceeded data for the untreated control (44.2 and 47.23 % regarding pollen germination and pollen tube elongation, respectively), as well as the effect of the control organic algal fertilizer Biofa (17.5 and 27.9 %, respectively). Pollen grains were inoculated in four culture media. A medium containing 15% sucrose and 1% agar had the most stimulating impact on pea pollen grains. Pollen viability, evaluated by staining with 1% carmine, was within limits of 74.72-87.97%. The highest viability of pollen grains was demonstrated after the application of Nagro organic nano-fertlizer.

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