scholarly journals Determination of fibernolytic enzyme activities of white rot fungi isolated from oil palm fronds

2016 ◽  
Vol 21 (2) ◽  
pp. 144
Author(s):  
Mohammad Azri bin Azmi ◽  
S. Alias ◽  
A.F.M. Azmi ◽  
A.A.A. Ghani ◽  
M.S. Shahudin ◽  
...  
Keyword(s):  
Oil Palm ◽  
Enzyme Activities ◽  
Animal Feed ◽  
White Rot Fungi ◽  
White Rot ◽  
Farm Animals ◽  
Full Potential ◽  
Degrading Enzyme ◽  
Oil Palm Fronds ◽  
Palm Fronds

<p>Oil palm fronds (OPF) is widely used as the source of roughage for the farm animals. However, the full potential of OPF as animal feed is limited by their high lignin content which limits the rumen microbe’s access to the cellulose and hemicellulose. White rot fungi (WRF) are a group of fungi belonging to basidiomycete phylum and are commonly found in decaying woody plant. They possess the ability to degrade lignin. This experiment aims to identify the phylum of the best lignin decaying fungi based on their enzymes activity. In this experiment, 11 fungi species were isolated from decaying oil palm fronds. They are labelled as WR1, WR 2, WR3, WR4, WR5, WR6, WR7, WR8, WR9, WR10 and WR11. Their fibernolytic enzyme activities which includes laccase, manganese peroxidase, lignin peroxidase, avicelase, carboxylmethylcellulase and xylanase are analysed using the solid state fermentation method. It <span style="text-decoration: line-through;">is</span> was found that 5 fungi species which are the WR1, WR2, WR4, WR7 and WR10 produced the highest ratio of lignin degrading enzyme to cellulose and hemicellulose degrading enzyme. The fungi are then analysed under microscope to determine the phylum of the fungi. From the observation, the fungi are identified to belong to the phylum basidiomycetes due to presence of clamp connection.</p>

scholarly journals Improving Ruminal Degradibility Of Oil Palm Fronds Using Enzyme Extracts From White Rot Fungi

2020 ◽  
Author(s):  
Ahmad Fariz - Nicholas ◽  
H.A. - Hassim ◽  
A.F. - Nicholas ◽  
Marta - Lourenço ◽  
A.A - Dias ◽  
...  
Keyword(s):  
Oil Palm ◽  
Lentinula Edodes ◽  
Culture Media ◽  
White Rot Fungi ◽  
White Rot ◽  
Citrate Buffer ◽  
Rumen Degradation ◽  
Oil Palm Fronds ◽  
Palm Fronds

Abstract Background: Oil palm fronds (OPF) when pretreated with white rot fungi (WRF) shows increased rumen degradability but with significant biomass loss. Thus, effects of pre-treated OPF with enzyme extracts from WRF on rumen degradability were studied in vitro. The enzyme extracts were prepared by inoculating OPF with three WRF, i.e Ceriporiopsis subvermispora, Lentinula edodes and Ganoderma lucidum, for 15, 30 and 45 days with either ammonium sulphate, (NH4)2SO4 and sodium nitrate, NaNO3 added to the culture media for each inoculation period. After preparation of enzyme extracts, the enzyme activities were determined. OPF was then pre-treated with enzyme extracts in a citrate buffer (pH 5.0) in a forced air oven at 40 oC during 5 days. Further, the in vitro rumen degradation of OPF pre-treated with enzyme extracts, with respect to the short chain fatty acid (SCFA) production, was determined after 24 h incubation. Activity of lignolytic (laccase and MnP), cellulolytic (CMCase and avicelase) and hemicellulolytic (xylanase) enzymes were measured in all of the extracts irrespective on the inoculation period. Results: Treatment of OPF with enzyme extracts from G. lucidum after 45 days of inoculation showed a numerical increase (13%) in total SCFA and apparently rumen degradable carbohydrates (ARDC) after 24 h in vitro incubation, without any loss of biomass. However, this increase was not clearly correlated to results of the enzyme assays. Conclusion: This study indicates pre-treatment of OPF with enzyme extracts from specific WRF to be promising to enhance the ruminal degradability of OPF without simultaneous loss of biomass.

Rumen degradation of oil palm fronds is improved through pre-digestion with white rot fungi but not through supplementation with yeast or enzymes

2012 ◽  
Vol 92 (1) ◽  
pp. 79-87 ◽  
Author(s):  
H. A. Hassim ◽  
M. Lourenço ◽  
Y. M. Goh ◽  
J. J. P. Baars ◽  
V. Fievez
Keyword(s):  
Oil Palm ◽  
Lentinula Edodes ◽  
White Rot Fungi ◽  
Gas Production ◽  
White Rot ◽  
Production Test ◽  
Rumen Degradation ◽  
Oil Palm Fronds ◽  
Palm Fronds

Hassim, H. A., Lourenço, M., Goh, Y. M., Baars, J. J. P. and Fievez, V. 2012. Rumen degradation of oil palm fronds is improved through pre-digestion with white rot fungi but not through supplementation with yeast or enzymes. Can. J. Anim. Sci. 92: 79–87. Rumen fermentation kinetics of oil palm fronds (OPF) supplemented or not with enzymes (Hemicell® or Allzyme SSF®) or yeasts (Levucell®SC or Yea-Sacc®) were studied through an in vitro gas production test (96 h) (exp. 1). In exp. 2, enzymes were supplemented to OPF pre-treated during 3 or 9 wk with either one of five white rot fungi strains. Yeasts and enzymes were tested both in active and inactive forms, which revealed the most appropriate set-up to distinguish between the rate of supplements as direct contributors to the fermentation substrate vs. stimulators of the fermentation of the basal substrate. In exp 1, addition of active and inactive Yea-Sacc® increased the apparently rumen degradable carbohydrates (ARDC) by 11%, whereas enzymes did not affect rumen degradability of non-inoculated OPF. Neither yeast nor enzymes influenced the rate of gas production of non-inoculated OPF, except for active Hemicell® at the low dose. In exp. 2, inoculation of OPF with Ceriporiopsis subvermispora for 3 wk and Lentinula edodes for 9 wk increased ARDC, but additional enzyme supplementation did not further improve ARDC or the rate of gas production.

Improving ruminal degradability of oil palm fronds using white rot fungi

2011 ◽  
Vol 169 (3-4) ◽  
pp. 157-166 ◽  
Author(s):  
M.M. Rahman ◽  
M. Lourenço ◽  
H.A. Hassim ◽  
J.J.P. Baars ◽  
A.S.M. Sonnenberg ◽  
...  
Keyword(s):  
Oil Palm ◽  
White Rot Fungi ◽  
White Rot ◽  
Oil Palm Fronds ◽  
Ruminal Degradability ◽  
Palm Fronds

scholarly journals Management of Oil Palm’s Residues for Utilization: Reduced Amount of Greenhouse Gas

2018 ◽  
Vol 11 (5) ◽  
pp. 96
Author(s):  
Wisakha Phoochinda
Keyword(s):  
Greenhouse Gas ◽  
Oil Palm ◽  
Greenhouse Gas Emission ◽  
Animal Feed ◽  
World Market ◽  
Gas Emission ◽  
Oil Palm Fronds ◽  
Policies And Measures ◽  
Palm Fronds ◽  
Palm Bunch

As the demand of oil palm outputs in the world market and Thailand has increased, it results in the oil palm&lsquo;s residues which need to be utilized. The study aimed to investigate the utilization of oil palm&rsquo;s residues and analyze the greenhouse gas emission from the utilization of oil palm&rsquo;s residues. The study reviewed related literature to obtain concepts, theories, research works, policies, and measures related to the utilization of oil palm&rsquo;s residues and interviewed to relevant scholars and agencies. The greenhouse gas emission on the utilization of oil palm&rsquo;s residues were calculated and then compared. The study findings revealed the utilization of the residues and the emission of greenhouse gas as follows: cultivation of straw mushroom from empty palm bunch emitted greenhouse gas 323.1264 KgCO2eq /year; use of oil palm fronds as animal feed emitted greenhouse gas 109.674 KgCO2eq /year; use of empty palm bunch to cover soil emitted greenhouse gas in total 109.674 KgCO2eq /year; composting from residues from palm oil extracting plants emitted greenhouse gas 210.346 KgCO2eq /year, compilation of oil palm fronds in heaps revealed no greenhouse gas emission, and composting from palm bunch emitted greenhouse gas 210.346 KgCO2eq /year.

A Utilization of Choline Chloride-Based Deep Eutectic Solvent Integrated with Alkaline Earth Metal Hexahydrate in the Pretreatment of Oil Palm Fronds

2021 ◽  
Vol 60 (5) ◽  
pp. 2011-2026
Author(s):  
Eng Kein New ◽  
Ta Yeong Wu ◽  
Khai Shing Voon ◽  
Alessandra Procentese ◽  
Katrina Pui Yee Shak ◽  
...  
Keyword(s):  
Oil Palm ◽  
Alkaline Earth ◽  
Choline Chloride ◽  
Deep Eutectic Solvent ◽  
Earth Metal ◽  
Alkaline Earth Metal ◽  
Oil Palm Fronds ◽  
Palm Fronds

scholarly journals Enzyme degradation mechanism of white rot fungi and its research progress on Refractory Wastewater

2021 ◽  
Vol 237 ◽  
pp. 01002
Author(s):  
ShiYuan Huang ◽  
Sheng Li ◽  
ZhenYu Wang ◽  
SenHuan Lin ◽  
Jian Deng
Keyword(s):  
Phenolic Compounds ◽  
Enzymatic Degradation ◽  
Enzyme System ◽  
Degradation Mechanism ◽  
White Rot Fungi ◽  
White Rot ◽  
Research Progress ◽  
Degrading Enzyme ◽  
Research Status ◽  
Enzyme Degradation

The lignin-degrading enzyme system of white rot fungi is highly efficient and non-specific, and can degrade a variety of pollutants, including dyes, phenolic compounds and pesticides.The article presents an overview of the mechanism of enzymatic degradation of white rot fungi and its research status in several refractory wastewater were described.

scholarly journals Characteristics of Activated Carbon from Oil Palm Fronds with the Addition of Sodium Carbonate (Na2CO3) and Sodium Chloride (NaCl) as an Activator

2019 ◽  
Vol 1 (1) ◽  
pp. 30-35
Author(s):  
Seri Maulina ◽  
Gewa Handika
Keyword(s):  
Activated Carbon ◽  
Sodium Chloride ◽  
Sodium Carbonate ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Activation Temperature ◽  
Spectra Analysis ◽  
Oil Palm Fronds ◽  
The Difference ◽  
Palm Fronds

This paper aims to understand the difference in characteristics of activated carbon produced from oil palm fronds (Elaeis guineensis Jacq) through the addition of two different activators, namely sodium carbonate (Na2CO3) and sodium chloride (NaCl). To do this, activator concentration of 10 percent each with activation temperature of 600 oC were applied in the experiment. Moreover, to determine the quality of activated carbon produced, a morphological analysis of activated carbon surfaces as well as FTIR spectra analysis on activated carbon. Identification using FTIR spectrophotometer revealed that the activated carbon in this study contained functional groups of O-H, C = O, C = C, C-C, and C-H.

Sign in / Sign up

Export Citation Format

Share Document