Enhanced production of β- glucosidase by locally isolated fungal strain employing submerged fermentation

β-glucosidase has wide spectrum of biotechnological applications in different industries including food, textile, laundry detergents, pulp and paper, pharmaceutical and biofuel industry. The present investigation related to isolation, screening, and process optimization of fungal strain for enhanced production of β-glucosidase (BGL). For this purpose, different fungal stains were isolated from different sources including soil, fruits, bark of tree as well as from the compost. The screening of fungal strain for BGL production was carried out via submerged fermentation. All the tested strains were identified on the basis of micro and macroscopic features. The fungal strain having greater ability for BGL synthesis among tested ones was identified as Aspergillus niger and given the code SBT-15. The process parameter including fermentation media, temperature, pH, rate of fermentation, carbon and nitrogen sources, volume of media were optimized. Five different fermentation media were evaluated M3 medium gave maximum production. The optimal conditions for BGL production was 72 hours of incubation at 40°C, pH 6 and 50 ml fermentation medium. Glucose (1%) and ammonium sulphate (3%) were optimized as best carbon and nitrogen sources, respectively.

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Carbon and Nitrogen Sources Effect on Pectinase Synthesis by Aspergillus niger Under Submerged Fermentation

Biosciences Biotechnology Research Asia ◽

10.13005/bbra/2906 ◽

2021 ◽

Vol 18 (1) ◽

pp. 185-195

Author(s):

Gousiya Begum ◽

Srinivas Munjam

Keyword(s):

Wheat Bran ◽

Submerged Fermentation ◽

Enzyme Production ◽

Paper Industry ◽

Nitrogen Sources ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Maximum Production ◽

Fungal Strains ◽

Enhanced Production

Pectinases are the commercial enzymes that are abundantly employed in various industries like fruit juice industries for clarification, wine indutsry and paper industry for bleaching up pulp. The present work was done on culture conditions optimization for production of pectinases under submerged fermentation using wheat bran as a substrate. Fungal strains were isolated from vegetable waste dump yard soils of Warangal district of Telangana state and screened for their activity on pectin agar medium. Among 30 isolates, two fungal strains showed good activity and identified them as A. niger and A. flavus. The effects of the different carbon and nitrogen sources on pectinases viz. exo-PG, endo-PG, endo-PL and PME by A. niger with 1% wheat bran was carried out in submerged fermentation. These studies revealed that carbon and nitrogen sources have shown considerable influence on enzyme production. Among all the carbon sources tried, sucrose at 1% was shown to be efficient carbon source for all four types of pectinases under investigation. For endo-PG, endo-PL and PME maximum enzyme production were recorded on 8th day of incubation period but for exo-PG enhanced production was observed on 12th day. A. niger could not produce PME on 12th day from 2.50% to subsequent concentrations. Among nine different nitrogen sources were screened, maximum pectinase production was recorded in sodium nitrate at 0.2 % for A. niger. Endo-PG, endo-PL and PME maximum production were recorded on 8th day of incubation and for exo-PG maximum production was observed on 12th day. No PME production was observed in A. niger on 12th day.

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Optimization of carbon and nitrogen sources in the medium and environmental factors for enhanced production of chitinase by Trichoderma harzianum

Bioprocess Engineering ◽

10.1007/bf00435522 ◽

1996 ◽

Vol 15 (1) ◽

pp. 13-20 ◽

Cited By ~ 17

Author(s):

A. Kapat ◽

S. K. Rakshit ◽

T. Panda

Keyword(s):

Environmental Factors ◽

Trichoderma Harzianum ◽

Nitrogen Sources ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Enhanced Production

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Studies on Optimization of Growth Parameters for L-Asparaginase Production byStreptomyces ginsengisoli

The Scientific World JOURNAL ◽

10.1155/2014/895167 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6 ◽

Cited By ~ 15

Author(s):

Neelima Deshpande ◽

Prachi Choubey ◽

Manasi Agashe

Keyword(s):

Anticancer Activity ◽

Submerged Fermentation ◽

Shake Flask ◽

Growth Parameters ◽

Nitrogen Sources ◽

Culture Conditions ◽

Carbon And Nitrogen Sources ◽

Multiple Functions ◽

Carbon And Nitrogen ◽

Maximal Production

A species ofStreptomyces,Streptomyces ginsengisoli, a river isolate, was evaluated for production of an enzyme, L-asparaginase, with multiple functions mainly anticancer activity. The actinomycete was subjected to submerged fermentation by “shake flask” method. The quantity of L-asparaginase produced was estimated as 3.23 μmol/mL/min. The effect of various culture conditions on L-asparaginase production was studied by adopting a method of variation in one factor at a time. Of the various conditions tested, glucose (followed by starch) and peptone served as good carbon and nitrogen sources, respectively, for maximal production of enzyme at pH 8. The temperature of 30°C and an incubation period of 5 days with 0.05 g% asparagine concentration were found to be optimum for L-asparaginase production.

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Keratinase Production by Three Bacillus spp. Using Feather Meal and Whole Feather as Substrate in a Submerged Fermentation

Enzyme Research ◽

10.4061/2011/523780 ◽

2011 ◽

Vol 2011 ◽

pp. 1-7 ◽

Cited By ~ 27

Author(s):

Ana Maria Mazotto ◽

Rosalie Reed Rodrigues Coelho ◽

Sabrina Martins Lage Cedrola ◽

Marcos Fábio de Lima ◽

Sonia Couri ◽

...

Keyword(s):

Enzymatic Activity ◽

Soluble Protein ◽

Submerged Fermentation ◽

Nitrogen Sources ◽

Poultry Industry ◽

Feather Meal ◽

Carbon And Nitrogen Sources ◽

Gelatinase Activity ◽

Carbon And Nitrogen ◽

Bacillus Spp

Three Bacillus species (B. subtilis LFB-FIOCRUZ 1270, B. subtilis LFB-FIOCRUZ 1273, and B. licheniformis LFB-FIOCRUZ 1274), isolated from the poultry industry, were evaluated for keratinase production using feathers or feather meal as the sole carbon and nitrogen sources in a submerged fermentation. The three Bacillus spp. produced extracellular keratinases and peptidases after 7 days. Feather meal was the best substrate for keratinase and peptidase production in B. subtilis 1273, with 412 U/mL and 463 U/ml. The three strains were able to degrade feather meal (62–75%) and feather (40–95%) producing 3.9–4.4 mg/ml of soluble protein in feather meal medium and 1.9–3.3 mg/ml when feather medium was used. The three strains produced serine peptidases with keratinase and gelatinase activity. B. subtilis 1273 was the strain which exhibited the highest enzymatic activity.

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Experimental Investigations on the Effects of Carbon and Nitrogen Sources on Concomitant Amylase and Polygalacturonase Production by Trichoderma viride BITRS-1001 in Submerged Fermentation

Biotechnology Research International ◽

10.1155/2012/904763 ◽

2012 ◽

Vol 2012 ◽

pp. 1-8 ◽

Cited By ~ 10

Author(s):

Arotupin Daniel Juwon ◽

Ogunmolu Funso Emmanuel

Keyword(s):

Submerged Fermentation ◽

Culture Media ◽

Trichoderma Viride ◽

Nitrogen Sources ◽

Experimental Investigations ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Highly Active ◽

Commercial Carbon ◽

Polygalacturonase Production

The paper investigates the effects of different commercial carbon and nitrogen sources on the concomitant synthesis of amylase and polygalacturonase enzymes with the aim of optimizing them for maximal enzyme production. The microorganism used in this work was the fungus Trichoderma viride BITRS-1001, which had been previously identified as a highly active producer of amylase and polygalacturonase enzymes. The results showed that the different commercial carbon and nitrogen substrate significantly affected the concomitant syntheses of amylase and polygalacturonase in culture media supplemented with the different commercial carbon and nitrogen substrates. The result obtained suggested that for optimal and concomitant synthesis of the enzymes by Trichoderma viride BITRS-1001 in submerged fermentation, minimal medium supplemented with maltose and casein were the carbon and nitrogen substrates of choice.

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Raw Fermentation Media for Industrial Production of Bacterial Cellulose

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.821-822.1093 ◽

2013 ◽

Vol 821-822 ◽

pp. 1093-1097

Author(s):

Yin Chuan Chen ◽

Yan Bin Zhu ◽

Yong Lin ◽

Jun Wang

Keyword(s):

Bacterial Cellulose ◽

Network Structure ◽

High Purity ◽

Raw Materials ◽

Nitrogen Sources ◽

Carbon And Nitrogen Sources ◽

Chemical Substances ◽

Carbon And Nitrogen ◽

Chemical Fields ◽

Fermentation Media

As a novel nanofunctional material, bacterial cellulose (BC) has been widely applied in food, papermaking, medical, spinning, acoustics, and chemical fields owing to the high purity, the nanoscale fine network structure and etc. The production of BC is significantly affected by nutrients such as carbon and nitrogen sources. Particularly, the output, form and structure of BC are affected by ethanol, organic acids and other chemical substances. Cheaply available raw materials, including fruits, molasses and other natural ones, are emphasized in this review.

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