scholarly journals Cytogenetic characterization of Ameivula ocellifera (Spix, 1825) (Squamata, Teiidae) from the brazilian northeast

2020 ◽  
Vol 36 (3) ◽  
Author(s):  
MARCELO JOÃO SILVA ◽  
Flávia Manoela Galvão Cipriano ◽  
Ana Paula de Araújo Vieira ◽  
Maria Rita dos Santos Cândido ◽  
Tamaris Gimenez Pinheiro ◽  
...  
Keyword(s):  
Wide Distribution ◽  
Chromosomal Marker ◽  
Nucleolar Organizing Regions ◽  
Federal Institute ◽  
Heteromorphic Sex Chromosomes ◽  
Pair Number ◽  
Cytogenetic Characterization ◽  
Laboratory Procedures ◽  
Brazilian Northeast

Ameivula is as a new genus of Teiidae family that emerged after extensive revision of species that comprised the former complex of species called Cnemidophorus group. Its species has a wide distribution from the northeast of Brazil to northern Argentina. Cytogenetic studies in the Teiidae family have shown that karyotypical data are important tools in phylogenetic and systematic studies within this group allowing to determine the position of species in the family. Thus, this study aimed to describe the karyotype of Ameivula ocellifera (Spix, 1825) from Picos, Piauí state in the Brazilian Northeast. Specimens were collected from August 2014 to October 2015 using interception traps and pitfalls, mounted randomly along the Caatinga area. The animals were collected and transported to Federal Institute of Piauí, campus Picos, where was carried out all laboratory procedures. Individuals analyzed showed a diploid number of 2n = 50 for both sexes, with karyotype composed by 30 macrochromosomes and 20 microchromosomes of telocentric and subtelocentric types. There were no heteromorphic sex chromosomes in the studied specimens. C-band technique evidenced the heterochromatic blocks in pericentromeric and telomeric regions of chromosomes. The nucleolar organizing regions appeared as a simple unit located at the terminal portion of the long arm of chromosomal pair number 5. The chromosomal characteristics of A. ocellifera analyzed do not show divergences regarding individuals from other regions. However, the nucleolar organizing regions seems to be a good chromosomal marker that permits to distinguish the species already studied.

Clinical and molecular cytogenetic characterization of two cases of inverted duplication deletion 8p

2020 ◽  
pp. 41-42
Author(s):  
Д.А. Юрченко ◽  
М.Е. Миньженкова ◽  
Е.Л. Дадали ◽  
Н.В. Шилова
Keyword(s):  
Mental Retardation ◽  
Congenital Heart ◽  
Heart Defects ◽  
Clinical Manifestations ◽  
Formation Mechanisms ◽  
Agenesis Of Corpus Callosum ◽  
Molecular Cytogenetic ◽  
Inverted Duplication ◽  
Cytogenetic Characterization

Синдром инвертированной дупликации короткого плеча хромосомы 8 со смежной терминальной делециенй (inv dup del(8p), ORPHA 96092) - редкая хромосомная аномалия (ХА) с частотой 1/10000-1/30000 живорожденных. В статье представлены клинические и молекулярно-цитогенетические характеристики двух неродственных пациентов с синдромом inv dup del(8p) и уточнены механизмы формирования хромосомного дисбаланса. Inverted duplication deletion 8p syndrome (inv dup del(8p), ORPHA 96092) is a rare chromosomal abnormality with a frequency of 1:10,000 - 30,000 newborns. Clinical manifestations of this syndrome include mental retardation, facial anomalies, hypoplasia/agenesis of corpus callosum, scoliosis and/or kyphosis, hypotonia, congenital heart defects. The article presents the clinical and molecular cytogenetic characteristics of two patients with inv dup del (8p) syndrome and clarifies the formation mechanisms.

Demographic, clinical, and ancestry characterization of a large cluster of mucopolysaccharidosis IV A in the Brazilian Northeast region

2021 ◽  
Author(s):  
Simone Silva Santos‐Lopes ◽  
Jessica Maria Florêncio Oliveira ◽  
Denise Queiroga Nascimento ◽  
Yorran Hardman Araújo Montenegro ◽  
Sandra Leistner‐Segal ◽  
...  
Keyword(s):  
Large Cluster ◽  
Northeast Region ◽  
Brazilian Northeast

Seed morphology and cytogenetic characterization of an endangered wild cotton, Gossypium turneri Fryxell, from Mexico

2021 ◽  
Author(s):  
Karla Fabiola Yescas-Romo ◽  
Francisco Elizandro Molina-Freaner ◽  
Francisca Ramírez-Godina ◽  
M. Humberto Reyes-Valdés ◽  
Octavio Martínez ◽  
...  
Keyword(s):  
Seed Morphology ◽  
Wild Cotton ◽  
Cytogenetic Characterization

scholarly journals Prenatal diagnosis and molecular cytogenetic characterization of a de novo duplication of 15q24.3-q26.1

2018 ◽  
Vol Volume 11 ◽  
pp. 77-80 ◽  
Author(s):  
Isabel Ochando ◽  
Melanie Cristine Alonzo Martínez ◽  
Ana María Serrano ◽  
Antonio Urbano ◽  
Eduardo Cazorla ◽  
...  
Keyword(s):  
Prenatal Diagnosis ◽  
De Novo ◽  
Molecular Cytogenetic ◽  
Cytogenetic Characterization

scholarly journals Molecular and Cytogenetic Characterization of Wild Musa Species

PLoS ONE ◽  
2015 ◽  
Vol 10 (8) ◽  
pp. e0134096 ◽  
Author(s):  
Jana Čížková ◽  
Eva Hřibová ◽  
Pavla Christelová ◽  
Ines Van den Houwe ◽  
Markku Häkkinen ◽  
...  
Keyword(s):  
Cytogenetic Characterization ◽  
Musa Species

Cytogenetic characterization of a new human papillary thyroid carcinoma permanent cell line (GLAG-66)

1993 ◽  
Vol 67 (2) ◽  
pp. 117-122 ◽  
Author(s):  
P. Antonini ◽  
G. Linares ◽  
N. Gaillard ◽  
A.M. Venuat ◽  
M. Schlumberger ◽  
...  
Keyword(s):  
Papillary Thyroid Carcinoma ◽  
Thyroid Carcinoma ◽  
Cell Line ◽  
Papillary Thyroid ◽  
Permanent Cell Line ◽  
Permanent Cell ◽  
Cytogenetic Characterization

Molecular cytogenetic characterization of 1p deletions and 1;17 translocation breakpoints in neuroblastoma

1994 ◽  
Vol 77 (2) ◽  
pp. 157
Author(s):  
Nadine Van Roy ◽  
Geneviève Laureys ◽  
Ngan Ching Cheng ◽  
Ghislain Opdenakker ◽  
Rogier Versteeg ◽  
...  
Keyword(s):  
Molecular Cytogenetic ◽  
Translocation Breakpoints ◽  
Cytogenetic Characterization

scholarly journals Biosynthesis of anandamide and N-palmitoylethanolamine by sequential actions of phospholipase A2 and lysophospholipase D

2004 ◽  
Vol 380 (3) ◽  
pp. 749-756 ◽  
Author(s):  
Yong-Xin SUN ◽  
Kazuhito TSUBOI ◽  
Yasuo OKAMOTO ◽  
Takeharu TONAI ◽  
Makoto MURAKAMI ◽  
...  
Keyword(s):  
Biosynthetic Pathway ◽  
Brain Homogenate ◽  
Wide Distribution ◽  
Rat Tissues ◽  
Lysophospholipase D ◽  
Pla2 Enzyme ◽  
First Time ◽  
Hydrolysis Of ◽  
The Brain

Anandamide (an endocannabinoid) and other bioactive long-chain NAEs (N-acylethanolamines) are formed by direct release from N-acyl-PE (N-acyl-phosphatidylethanolamine) by a PLD (phospholipase D). However, the possible presence of a two-step pathway from N-acyl-PE has also been suggested previously, which comprises (1) the hydrolysis of N-acyl-PE to N-acyl-lysoPE by PLA1/PLA2 enzyme(s) and (2) the release of NAEs from N-acyllysoPE by lysoPLD (lysophospholipase D) enzyme(s). In the present study we report for the first time the characterization of enzymes responsible for this pathway. The PLA1/PLA2 activity for N-palmitoyl-PE was found in various rat tissues, with the highest activity in the stomach. This stomach enzyme was identified as group IB sPLA2 (secretory PLA2), and its product was determined as N-acyl-1-acyl-lysoPE. Recombinant group IB, IIA and V of sPLA2s were also active with N-palmitoyl-PE, whereas group X sPLA2 and cytosolic PLA2α were inactive. In addition, we found wide distribution of lysoPLD activity generating N-palmitoylethanolamine from N-palmitoyl-lysoPE in rat tissues, with higher activities in the brain and testis. Based on several lines of enzymological evidence, the lysoPLD enzyme could be distinct from the known N-acyl-PE-hydrolysing PLD. sPLA2-IB dose dependently enhanced the production of N-palmitoylethanolamine from N-palmitoyl-PE in the brain homogenate showing the lysoPLD activity. N-Arachidonoyl-PE and N-arachidonoyl-lysoPE as anandamide precursors were also good substrates of sPLA2-IB and the lysoPLD respectively. These results suggest that the sequential actions of PLA2 and lysoPLD may constitute another biosynthetic pathway for NAEs, including anandamide.

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