Expression of β-defensin gene in potato confers enhanced resistance to Ralstonia Solanacearum L.

2017 ◽  
Vol 3 (1) ◽  
pp. 15
Author(s):  
Meetul Kumar ◽  
Swarup Kumar Chakrabarti
Keyword(s):  
Disease Progression ◽  
Ralstonia Solanacearum ◽  
Northern Hybridization ◽  
Bacterial Wilt Disease ◽  
Life Cycle Stages ◽  
Percent Disease Index ◽  
Efficient Selection ◽  
Bacterial Plant Pathogen ◽  
Selection Of ◽  
Stem Segments

An optimized methodology of <em>Agrobacterium</em>-mediated stable genetic transformation of potato (<em>Solanum tuberosum </em>L.) using the shoot organogenesis potential of internodal stem segments for increased resistance to bacterial plant pathogen, <em>Ralstonia solanacearum</em> L. was developed. Improvised plant regeneration protocol for expression of antimicrobial β-defensin transgene and efficient selection of tissues in plant selectable marker, kanamycin sulphate was successfully utilized for transformation of potato.  Stable integration and expression of antimicrobial peptide was observed in plant tissues and validated by associated molecular analysis by RT PCR, Southern hybridization, northern hybridization and western blotting of the infected tissues. The bacterial wilt disease progression was monitored in controlled greenhouse and Percent Disease Index (PDI) was measured by analysis of variance (ANOVA) that selected superior resistant plants. These transformed plants were able to contain the disease progression and complete the life cycle stages and developed healthy tubers.

scholarly journals Study on the Response of some Solanaceous Plants to Ralstonia solanacearum Biovars 2A and 2T

2014 ◽  
Vol 3 (2) ◽  
pp. 80
Author(s):  
Vahideh Hasabi
Keyword(s):  
Plant Pathogen ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Wilt Disease ◽  
Bacterial Density ◽  
Hypersensitivity Response ◽  
Bacterial Wilt Disease ◽  
Temperature Regimes ◽  
Solanaceous Plants ◽  
Bacterial Plant Pathogen

Ralstonia solanacearum is a very destructive bacterial plant pathogen that causes wilt disease in solanaceae crops. To study the response of potato, tomato, eggplant and petunia to bacterial wilt disease, two isolates representing biovars 2A and 2T of R. solanacearum were evaluated for their pathogenicity aggressiveness and tobacco hypersensitivity response (HR) at two different temperature regimes. The response of plants was estimated by appearance of wilting symptoms and bacterial density in the xylems of inoculated plants over a four weeks period. The results indicated that isolates representing biovar 2T caused less disease in all the species and cultivars compared to isolates biovar 2A, at both temperature conditions and also, there were significant differences in susceptibility to biovars 2A and 2T of R. solanacearum among tomato, eggplant and petunia and potato cultivars.

scholarly journals Network Analysis Based on Unique Spectral Features Enables an Efficient Selection of Genomically Diverse Operational Isolation Units

2021 ◽  
Vol 9 (2) ◽  
pp. 416
Author(s):  
Charles Dumolin ◽  
Charlotte Peeters ◽  
Evelien De Canck ◽  
Nico Boon ◽  
Peter Vandamme
Keyword(s):  
Network Analysis ◽  
Hierarchical Clustering ◽  
Mass Spectra ◽  
Spectral Features ◽  
Maldi Tof ◽  
Maldi Tof Mass Spectra ◽  
Diversity Studies ◽  
Technical Sample ◽  
Efficient Selection ◽  
Selection Of

Culturomics-based bacterial diversity studies benefit from the implementation of MALDI-TOF MS to remove genomically redundant isolates from isolate collections. We previously introduced SPeDE, a novel tool designed to dereplicate spectral datasets at an infraspecific level into operational isolation units (OIUs) based on unique spectral features. However, biological and technical variation may result in methodology-induced differences in MALDI-TOF mass spectra and hence provoke the detection of genomically redundant OIUs. In the present study, we used three datasets to analyze to which extent hierarchical clustering and network analysis allowed to eliminate redundant OIUs obtained through biological and technical sample variation and to describe the diversity within a set of spectra obtained from 134 unknown soil isolates. Overall, network analysis based on unique spectral features in MALDI-TOF mass spectra enabled a superior selection of genomically diverse OIUs compared to hierarchical clustering analysis and provided a better understanding of the inter-OIU relationships.

Efficient selection of feature sets possessing high coefficients of determination based on incremental determinations

2003 ◽  
Vol 83 (4) ◽  
pp. 695-712 ◽  
Author(s):  
Ronaldo F. Hashimoto ◽  
Edward.R. Dougherty ◽  
Marcel Brun ◽  
Zheng-Zheng Zhou ◽  
Michael L. Bittner ◽  
...  
Keyword(s):  
Feature Sets ◽  
Efficient Selection ◽  
Selection Of

scholarly journals Ralstonia solanacearum Needs Motility for Invasive Virulence on Tomato

2001 ◽  
Vol 183 (12) ◽  
pp. 3597-3605 ◽  
Author(s):  
Julie Tans-Kersten ◽  
Huayu Huang ◽  
Caitilyn Allen
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Soft Agar ◽  
Cell Protein ◽  
In Planta ◽  
Tomato Plants ◽  
Bacterial Wilt Disease ◽  
Virulence Assay ◽  
Flagellar Filament

ABSTRACT Ralstonia solanacearum, a widely distributed and economically important plant pathogen, invades the roots of diverse plant hosts from the soil and aggressively colonizes the xylem vessels, causing a lethal wilting known as bacterial wilt disease. By examining bacteria from the xylem vessels of infected plants, we found thatR. solanacearum is essentially nonmotile in planta, although it can be highly motile in culture. To determine the role of pathogen motility in this disease, we cloned, characterized, and mutated two genes in the R. solanacearum flagellar biosynthetic pathway. The genes for flagellin, the subunit of the flagellar filament (fliC), and for the flagellar motor switch protein (fliM) were isolated based on their resemblance to these proteins in other bacteria. As is typical for flagellins, the predicted FliC protein had well-conserved N- and C-terminal regions, separated by a divergent central domain. The predicted R. solanacearum FliM closely resembled motor switch proteins from other proteobacteria. Chromosomal mutants lackingfliC or fliM were created by replacing the genes with marked interrupted constructs. Since fliM is embedded in the fliLMNOPQR operon, the aphAcassette was used to make a nonpolar fliM mutation. Both mutants were completely nonmotile on soft agar plates, in minimal broth, and in tomato plants. The fliC mutant lacked flagella altogether; moreover, sheared-cell protein preparations from the fliC mutant lacked a 30-kDa band corresponding to flagellin. The fliM mutant was usually aflagellate, but about 10% of cells had abnormal truncated flagella. In a biologically representative soil-soak inoculation virulence assay, both nonmotile mutants were significantly reduced in the ability to cause disease on tomato plants. However, the fliC mutant had wild-type virulence when it was inoculated directly onto cut tomato petioles, an inoculation method that did not require bacteria to enter the intact host from the soil. These results suggest that swimming motility makes its most important contribution to bacterial wilt virulence in the early stages of host plant invasion and colonization.

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