scholarly journals Resistance of Three Superior New Banana Varieties on Several Vegetative Compatibility Groups Fusarium oxysporum f. sp. cubense

2021 ◽  
Vol 17 (4) ◽  
pp. 151-158
Author(s):  
Jumjunidang No ◽  
Riska ◽  
Resta Patma Yanda ◽  
Titin Purnama ◽  
Agus Sutanto ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Vegetative Compatibility ◽  
Vegetative Compatibility Groups

Balai Penelitian Tanaman Buah (Balitbu) Tropika telah melepas tiga varietas unggul baru (VUB) pisang dengan keunggulan masing-masing. Dalam menunjang keberhasilan pengembangan varietas unggul baru tersebut perlu diketahui informasi tentang ketahanannya terhadap beberapa Vegetative Compatibility Groups (VCGs) Fusarium oxysporum f. sp. cubense (Foc). Tujuan penelitian ini ialah menentukan ketahanan tiga VUB pisang terhadap beberapa VCGs Foc di rumah kasa. Penelitian dilakukan di laboratorium proteksi tumbuhan dan rumah kasa Balitbu Tropika di Solok, dari bulan September 2016 sampai bulan Desember 2017. Rancangan yang digunakan ialah acak kelompok 21 perlakuan dan 3 ulangan, masing-masing perlakuan terdiri atas 10 tanaman. Sebanyak 3 VUB pisang (Ketan 01, Kepok Tanjung dan Raja Kinalun) masing-masing diinokulasi dengan 7 VCGs Foc: yaitu VCG 0120/15, 0123, 0124/5, 0126, 01218, 01213/16 dan 01219. Hasil penelitian menunjukkan bahwa tiga VUB pisang menunjukkan respons ketahanan yang bervariasi terhadap isolat Foc dari berbagai VCGs dalam ras 1 dan ras 4. Ketahanan dipengaruhi oleh kompatibilitas masing-masing VCGs dari isolat Foc dengan varietas pisang. Varietas Ketan 01 (AA) mempunyai respons agak tahan dan tahan terhadap semua VCGs Foc yang diuji kecuali dengan VCG 0124/5 (ras 1) dan VCG 01213/16 (ras 4 tropis). Kepok Tanjung (ABB/BBB) rentan dan sangat rentan terhadap semua VCGs Foc, Raja Kinalun (ABB) memberikan respons tahan dan sangat tahan terhadap semua isolat VCGs Foc kecuali dengan isolat Foc VCG 0124/5 (ras 1) responsnya sangat rentan. Hasil penelitian ini bermanfaat sebagai informasi awal pengembangan varietas unggul baru pisang di Indonesia.

scholarly journals Vegetative Compatibility Groups of Fusarium oxysporum f. sp. lactucae from Lettuce

Plant Disease ◽  
2005 ◽  
Vol 89 (3) ◽  
pp. 237-240 ◽  
Author(s):  
Matias Pasquali ◽  
Flavia Dematheis ◽  
Giovanna Gilardi ◽  
Maria Lodovica Gullino ◽  
Angelo Garibaldi
Keyword(s):  
Fusarium Oxysporum ◽  
Genetic Relatedness ◽  
The United States ◽  
Vegetative Compatibility ◽  
Vegetative Compatibility Group ◽  
Vegetative Compatibility Groups ◽  
Compatibility Group ◽  
Race 1

Fusarium oxysporum f. sp. lactucae, the causal agent of Fusarium wilt of lettuce, has been reported in three continents in the last 10 years. Forty-seven isolates obtained from infected plants and seed in Italy, the United States, Japan, and Taiwan were evaluated for pathogenicity and vegetative compatibility. Chlorate-resistant, nitrate-nonutilizing mutants were used to determine genetic relatedness among isolates from different locations. Using the vegetative compatibility group (VCG) approach, all Italian and American isolates, type 2 Taiwanese isolates, and a Japanese race 1 were assigned to the major VCG 0300. Taiwanese isolates type 1 were assigned to VCG 0301. The hypothesis that propagules of Fusarium oxysporum f. sp. lactucae that caused epidemics on lettuce in 2001-02 in Italian fields might have spread via import and use of contaminated seeds is discussed.

Analysis of Vegetative Compatibility Groups of Fusarium oxysporum from Eruca vesicaria and Diplotaxis tenuifolia

2007 ◽  
Vol 155 (1) ◽  
pp. 61-64 ◽  
Author(s):  
A. Catti ◽  
M. Pasquali ◽  
D. Ghiringhelli ◽  
A. Garibaldi ◽  
M. L. Gullino
Keyword(s):  
Fusarium Oxysporum ◽  
Vegetative Compatibility ◽  
Vegetative Compatibility Groups

Vegetative compatibility groups within Fusarium oxysporum f.sp. lini from Linum usitatissimum (flax) wilt nurseries in western Canada

2001 ◽  
Vol 79 (7) ◽  
pp. 836-843 ◽  
Author(s):  
S I Mpofu ◽  
K Y Rashid
Keyword(s):  
Population Structure ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Linum Usitatissimum ◽  
Vegetative Compatibility ◽  
Western Canada ◽  
Disease Development ◽  
Vegetative Compatibility Groups ◽  
Linum Usitatissimum L ◽  
Compatibility Analysis

Following the discovery of substantial differences in the development and severity of Fusarium wilt in Linum usitatissimum L. (flax) wilt nurseries in western Canada, a study of the population structure of Fusarium oxysporum f.sp. lini (Bolley) Snyd. & Hans using vegetative compatibility analysis was initiated. Vegetative compatibility was determined using nitrate non-utilizing mutants. From a total of 105 isolates, 74 were assigned to 12 vegetative compatibility groups (VCG 0440-04411), 22 were not compatible with any other isolates and 9 did not produce mutants. The populations of F. oxysporum f.sp. lini in Fusarium wilt nurseries in western Canada were significantly different. There was a predominant VCG in each of the nurseries, which was either nonexistent or not common in other nurseries, VCG 0440 in Indian Head, 0441 in Treherne, 0442 in Morden-80, 0443 in Saskatoon, and 0444 in Morden-60. There were only three overlapping VCGs; VCG 0440 was common to Morden-60 and Indian Head and VCGs 0442 and 0444 were common to Morden-60 and Morden-80. The differences in disease development and severity observed previously may be due to the differences in the population structure of the pathogen. These findings have significant implications for the approaches to breeding for Fusarium wilt resistance in flax.Key words: Fusarium oxysporum f.sp. lini, flax, Fusarium wilt, genetic diversity, vegetative compatibility.

Vegetative compatibility groupings in a local population of Fusarium oxysporum

1991 ◽  
Vol 69 (1) ◽  
pp. 168-172 ◽  
Author(s):  
T. R. Gordon ◽  
D. Okamoto
Keyword(s):  
Fusarium Oxysporum ◽  
Key Words ◽  
Local Population ◽  
San Joaquin Valley ◽  
Vegetative Compatibility ◽  
Vegetative Compatibility Groups ◽  
Heterokaryon Formation ◽  
Pathogenicity Tests

One hundred isolates of Fusarium oxysporum collected from soil in the San Joaquin Valley in 1988 were tested for their ability to form intra- and inter-isolate heterokaryons. Five thousand and fifty isolate combinations were tested for vegetative compatibility, using complementary nitrate nonutilizing mutants. Ninety-eight combinations (2%) produced weakly positive reactions, 248 (5%) produced strong reactions, and 4704 combinations (93%) were negative for heterokaryon formation. This result suggests that anastomosis occurs infrequently among isolates in this population. Isolates were assigned to vegetative compatibility groups based on strong reactions. By this procedure 100 isolates were placed in 29 vegetative compatibility groups. At least one isolate from each of the 29 vegetative compatibility groups was tested and found to be vegetatively incompatible with the muskmelon wilt pathogen, F. oxysporum f. sp. melonis, which occurs in the same field. Each of these isolates also was avirulent to muskmelon in greenhouse pathogenicity tests. Forty isolates of F. oxysporum were collected in 1989 and 32 (80%) were compatible with isolates collected in 1988. The remaining eight isolates represented eight different vegetative compatibility groups. Key words: anastomosis, fungi, heterokaryon.

scholarly journals Biological and Molecular Characterization of Fusarium oxysporum f. sp. lycopersici Divides Race 1 Isolates into Separate Virulence Groups

1999 ◽  
Vol 89 (2) ◽  
pp. 156-160 ◽  
Author(s):  
Jurriaan J. Mes ◽  
Emma A. Weststeijn ◽  
Frits Herlaar ◽  
Joep J. M. Lambalk ◽  
Jelle Wijbrandi ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Random Amplified Polymorphic Dna ◽  
Vegetative Compatibility ◽  
Avirulence Gene ◽  
Resistance Response ◽  
Vegetative Compatibility Groups ◽  
Small Set ◽  
Race 1 ◽  
Race 2 ◽  
Susceptible Tomato

A collection of race 1 and race 2 isolates of Fusarium oxysporum f. sp. lycopersici was screened for vegetative compatibility and characterized by random amplified polymorphic DNA (RAPD) analysis to establish the identity and genetic diversity of the isolates. Comparison of RAPD profiles revealed two main groups that coincide with vegetative compatibility groups (VCGs). In addition, several single-member VCGs were identified that could not be grouped in one of the two main RAPD clusters. This suggests that F. oxysporum f. sp. lycopersici is a polyphyletic taxon. To assign avirulence genotypes to race 1 isolates, they were tested for their virulence on a small set of tomato lines (Lycopersicon esculentum), including line OT364. This line was selected because it shows resistance to race 2 isolates but, unlike most other race 2-resistant lines, susceptibility to race 1 isolates. To exclude the influence of other components than those related to the race-specific resistance response, we tested the virulence of race 1 isolates on a susceptible tomato that has become race 2 resistant by introduction of an I-2 transgene. The results show that both line OT364 and the transgenic line were significantly affected by four race 1 isolates, but not by seven other race 1 isolates nor by any race 2 isolates. This allowed a subdivision of race 1 isolates based on the presence or absence of an avirulence gene corresponding to the I-2 resistance gene. The data presented here support a gene-for-gene relationship for the interaction between F. oxysporum f. sp. lycopersici and its host tomato.

Analysis of vegetative compatibility groups in nonpathogenic populations of Fusarium oxysporum isolated from symptomless tomato roots

1991 ◽  
Vol 69 (10) ◽  
pp. 2089-2094 ◽  
Author(s):  
Karol S. Elias ◽  
R. W. Schneider ◽  
M. M. Lear
Keyword(s):  
Fusarium Oxysporum ◽  
Vegetative Compatibility ◽  
Vegetable Crops ◽  
Tomato Production ◽  
Vegetative Compatibility Groups ◽  
Horticultural Traits ◽  
Tomato Roots ◽  
Constant State ◽  
Nitrate Metabolism ◽  
Tomato Cultivars

A collection of 471 isolates of Fusarium oxysporum was obtained from symptomless tomato roots of plants grown at eight locations in Louisiana. Isolates that produced nitrate metabolism mutants (317 isolates) were included in an analysis of vegetative compatibility groups. One hundred and eighty-six isolates were assigned to 48 vegetative compatibility groups (2015–2062) containing 2–15 members from one to three different collection sites. Even though each site contained sub-populations of F. oxysporum common to other sites, only about half of the vegetative compatibility groups (21 groups) contained isolates from more than one site. In addition, there were 131 isolates of F. oxysporum that formed single-member vegetative compatibility groups. This diverse population composition is probably the result of several factors, including breeding strategies for resistance to fusarium wilt and tomato production practices in Louisiana. Resistance genes and horticultural traits from several sources (Lycopersicon spp.) have been incorporated into commercial tomato cultivars currently being grown. In addition, the long growing season in Louisiana allows several tomato cultivars containing different genes for resistance to be grown consecutively in the same field in a single year. Furthermore, other vegetable crops may be alternated with the tomato crops. Thus, the selection pressures that influence the composition of the nonpathogenic populations of F. oxysporum are in a constant state of flux. Key words: Fusarium oxysporum, genetic diversity, nonpathogens, vegetative compatibility groups.

Further investigations of vegetative compatibility within Fusarium oxysporum f.sp. melonis

1990 ◽  
Vol 68 (6) ◽  
pp. 1245-1248 ◽  
Author(s):  
D. J. Jacobson ◽  
T. R. Gordon
Keyword(s):  
Genetic Diversity ◽  
North America ◽  
Fusarium Oxysporum ◽  
Vegetative Compatibility ◽  
Vegetative Compatibility Group ◽  
Culture Collections ◽  
Vegetative Compatibility Groups ◽  
The Third ◽  
Geographic Origins ◽  
Compatibility Group

One hundred and nineteen strains of Fusarium oxysporum f.sp. melonis were characterized by virulence and vegetative compatibility. One hundred and seven strains were placed in four previously reported vegetative compatibility groups: 0130, 0131, 0133, and 0134. Four strains were placed in three new vegetative compatibility groups, and the remaining eight strains were vegetatively self-incompatible. Two of the three new vegetative compatibility groups shared similar geographic origins and distribution with two previously reported vegetative compatibility groups; the third represented a more isolated infestation. All vegetatively self-incompatible isolates originated from culture collections; none have been recently isolated from nature. These newly characterized strains extend our knowledge of genetic diversity in F. oxysporum f.sp. melonis. All four F. oxysporum f.sp. melonis races exist in more than one vegetative compatibility group. European strains represent four vegetative compatibility groups, one of which is present in North America and another in the Middle East. The significance of this diversity is unknown, as are the phylogenetic relationships among strains in this forma specialis.

Sign in / Sign up

Export Citation Format

Share Document