scholarly journals CONVERSION OF PINEAPPLE JUICE WASTE INTO LACTIC ACID IN BATCH AND FED – BATCH FERMENTATION SYSTEMS

REAKTOR ◽  
2008 ◽  
Vol 12 (2) ◽  
pp. 98 ◽  
Author(s):  
Abdullah Mochamad Busairi
Keyword(s):  
Lactic Acid ◽  
Batch Culture ◽  
Lactic Acid Production ◽  
Raw Material ◽  
Fed Batch ◽  
Feed Concentration ◽  
Pineapple Juice ◽  
Acid Productivity ◽  
Lactic Acid Productivity ◽  
Fed Batch Culture

Pineapple juice waste contains valuable components, which are mainly sucrose, glucose, and fructose. Recently, lactic acid has been considered to be an important raw material for the production of biodegradable lactide polymer. The fermentation experiments were carried out in a 3 litres fermentor (Biostat B Model) under anaerobic condition with stirring speed of 50 rpm, temperature at 40oC, and pH of 6.00. Effect of feed concentration on lactic acid production, bacterial growth, substrate utilisation and productivity was studied. The results obtained from fed- batch culture fermentation showed that the maximum lactic acid productivity was 0.44 g/L.h for feed concentration of 90 g/L at 48 hours. Whereas the lactic acid productivity obtained from fed-batch culture was twice and half fold higher than that of batch culture productivity.  Buangan jus nanas mengandung komponen yang berharga terutama sukrosa, glukosa, dan fruktosa. Asam laktat adalah bahan baku yang terbaru dan penting untuk dibuat sebagai polimer laktat yang dapat terdegradasi oleh lingkungan. Percobaan dilakukan pada fermentor 3 liter (Model Biostat B) di bawah kondisi anaerob dengan kecepatan pengadukan 50 rpm, temperatur 40oC, dan pH 6,00. Pengaruh konsentrasi umpan terhadap produksi asam laktat, pertumbuhan mikroba, pengggunaan substrat dan produktivitas telah dipelajari. Hasil yang didapatkan pada fermentasi dengan menggunakan sistem fed-batch menunjukkan bahwa produktivitas asam laktat maksimum adalah 0.44 g/L,jam dengan konsentrasi umpan, 90 g/L pada waktu 48 jam. Bahkan produktivitas asam laktat yang didapat pada kultur fed-batch lebih tinggi 2,5 kali dari pada proses menggunakan sistem batch

scholarly journals High Improvement in Lactic Acid Productivity by New Alkaliphilic Bacterium Using Repeated Batch Fermentation Integrated with Increased Substrate Concentration

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Mohamed Ali Abdel-Rahman ◽  
Saad El-Din Hassan ◽  
Mohamed Salah Azab ◽  
Abdullah-Al- Mahin ◽  
Mahmoud Ali Gaber
Keyword(s):  
Lactic Acid ◽  
Batch Fermentation ◽  
Lactic Acid Production ◽  
Cost Effective ◽  
Volumetric Productivity ◽  
Rrna Gene ◽  
Acid Productivity ◽  
Wide Range ◽  
Lactic Acid Productivity ◽  
Alkaliphilic Bacterium

Optically pure lactic acid (LA) is an important chemical platform that has a wide range of industrial and biotechnological applications. Improved parameters for cost effective LA production are of great interest for industrial developments. In the present study, an alkaliphilic lactic acid bacterium, BoM 1-2, was selected among 369 newly obtained bacterial isolates. It was characterized using API 50 CHL kit and identified asEnterococcus hiraeBoM 1-2 by 16S rRNA gene sequence analysis. Efficient polymer-gradeL-lactic acid production was achieved at pH 9.0 and 40°C. In batch fermentation strategy using 20 g L−1glucose, 19.6 g L−1lactic acid was obtained with volumetric productivity of 2.18 g L−1 h−1. While using 100 g L−1glucose, 96.0 g L−1lactic acid was obtained with volumetric productivity of 1.07 g L−1 h−1.The highest lactic acid concentration of 180.6 g L−1was achieved in multipulse fed batch strategy with volumetric productivity of 0.65 g L−1 h−1. To achieve higher productivity, repeated fermentation processes were applied using the two different strategies. In the first strategy, the lactic acid productivity was increased from 1.97 g L−1 h−1to 4.48 g L−1 h−1when the total of 10 repeated runs were carried out using 60 g L−1glucose, but lactic acid productivity decreased to 2.95 g L−1 h−1using 100 g L−1glucose. In second strategy, repeated fermentation coupled with gradual increase in glucose concentration from 40 to 100 g L−1was conducted for 24 runs. A dramatic increase in LA productivity up to 39.9 g L−1 h−1(18-fold compared to first run) was achieved using 40 g L−1glucose while volumetric productivity ranging between 24.8 and 29.9 g L−1 h−1was achieved using 60–100 g L−1glucose.

Suppression of lactate production in fed-batch culture of some lactic acid bacteria with sucrose as the carbon source

2020 ◽  
Vol 129 (5) ◽  
pp. 535-540 ◽  
Author(s):  
Mio Kawai ◽  
Asami Tsuchiya ◽  
Junya Ishida ◽  
Nobuo Yoda ◽  
Shino Yashiki-Yamasaki ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Carbon Source ◽  
Batch Culture ◽  
Lactate Production ◽  
Fed Batch ◽  
Fed Batch Culture
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