Comparison of pregnancy rates following fresh and frozen embryo transfer in women undergoing assisted reproductive techniques

Freezing embryos is the best way to increase fertility for women with ovarian hyper-stimulation syndrome and women at risk for ovarian dysfunction. Due to the importance of freezing and pregnancy embryos, the present study was conducted to compare the fertility rate and the affecting factors following fresh embryos and frozen embryos in women treated with assisted reproductive techniques. In this study, 250 infertile women and IVF/ICSI candidates were studied. Embryos were used in fresh or frozen groups for transfer to the uterus. The expression of the caspase-3 gene was also examined for further evaluation. Data analysis was performed using SPSS 16 software, Chi-square, independent t-test, and Kruskal-Wallis tests. Out of 250 infertile women, 96 (38.4%) became pregnant, of which 54 were in the fresh embryo group and 42 were in the frozen embryo group, which was not statistically significant (P=0.32). Infertility causes, number of embryonic cells and grading of transferred embryos, delivery complications, embryo implantation methods, number of produced embryos after delivery, and endometrial thickness were not significantly different between the two groups (P=0.53). The difference between the mean number of transferred embryos in the two groups was significant (P<0.05), which was no longer significant after excluding non-pregnant women, and in comparing with only pregnant women (P=0.36). The result of caspase-3 gene expression showed that there was significant differences between fresh embryos, healthy thawed frozen embryos, and destroyed thawed frozen embryos. But these results were totally different from the results of the Pregnancy rate section. Therefore, it is inferred that although caspase-3 genes are expressed in frozen embryos after thawing and are ready to destroy the embryo, there are probably a number of involved factors that prevent the activity of caspase-3 and do not allow the apoptotic process to occur. What these factors are and how they prevent this process needs further study.

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Perception of Prenatal Sex Selection among Women Attending Antenatal Clinic in a South Western Nigerian Town

Babcock University Medical Journal (BUMJ) ◽

10.38029/bumj.v1i1.23 ◽

2015 ◽

Vol 1 (1) ◽

pp. 29-35

Author(s):

J Imaralu ◽

FI Ani ◽

AO Olaleye ◽

JO Sotunsa ◽

OO Adelowo

Keyword(s):

Pregnant Women ◽

Embryo Transfer ◽

Tertiary Education ◽

Antenatal Clinic ◽

Legal Regulation ◽

Sex Selection ◽

Male Child ◽

Assisted Reproductive Techniques ◽

Ogun State ◽

Reproductive Techniques

Objective: To determine the perception of child sex selection and the factors affecting acceptance of assisted reproductive techniques for child sex selection among pregnant women in Ogun state, Southwestern Nigeria. Methods: A cross sectional survey conducted among 400 antenatal clinic attendees in the three largest hospitals in Sagamu and its environs in Ogun state. Results: Participants were mostly Yorubas (83.1%), Christians (71.5%) and had tertiary education (52.8%). The male child was preferred among respondents who indicated their choice (84.8%). Relative subfertility before index conception influenced the preference for a male child (p<0.001); it however, did not have any significant influence on awareness of methods of prenatal sex selection (p=0.965, CI=0.960-0.969). Presence of existing male children had a significantly negative effect on preferred child sex (p=0.377, CI=0.365-0.390). In-vitro fertilization with embryo transfer (71.3%) is the method of assisted conception most known to the respondents while selective embryo transfer is the commonest method of prenatal sex selection they are aware of (42.6%). Educational level (p<0.001), ethnicity (p<0.05) and religion (p<0.001), determined the acceptance of prenatal sex selection. Most (77.8%) of the respondents who welcomed the idea, would undergo procedures to have a male child; a choice which however did not significantly influence their opinion on legal regulation of these procedures. Conclusion: Pregnant women in Sagamu are mostly aware of assisted reproductive techniques for prenatal sex selection and would consider them for difficulty in bearing male offspring. This choice however did not significantly affect their attitudes towards legal restrictions of sex selective procedures.

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Efficacy of Group Reality Therapy on Anxiety in Infertile Women Undergoing Treatment with Assisted Reproductive Techniques IUI

Mediterranean Journal of Social Sciences ◽

10.5901/mjss.2016.v7n3s3p127 ◽

2016 ◽

Author(s):

Habibeh Soltanzadeh Mezreji ◽

Hassan Toozandehjani

Keyword(s):

Assisted Reproductive Techniques ◽

Reality Therapy ◽

Infertile Women ◽

Reproductive Techniques

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Laparoscopic Surgery and Assisted Reproductive Techniques Combined Strategies of Therapy in Infertile Women

Annals of the New York Academy of Sciences ◽

10.1111/j.1749-6632.1997.tb48552.x ◽

1997 ◽

Vol 828 (1 Uterus, The) ◽

pp. 316-325 ◽

Cited By ~ 3

Author(s):

RENATO SERACCHIOLI ◽

MARIA RITA TREVISI ◽

FEDERICA FERLINI ◽

SIMONE PETRACCHI ◽

CLAUDIA COLOMBI ◽

...

Keyword(s):

Laparoscopic Surgery ◽

Assisted Reproductive Techniques ◽

Infertile Women ◽

Reproductive Techniques

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Impact of Assisted Reproductive Technology on Embryonic Health Screening Tests: A Case-Control Study

hormozgan medical journal ◽

10.5812/hmj.103645 ◽

2020 ◽

Vol In Press (In Press) ◽

Author(s):

Shirin Soltani ◽

Akbar Karimi ◽

Zahra Khashavi ◽

Nasibeh Roozbeh

Keyword(s):

Pregnant Women ◽

First Trimester ◽

Assisted Reproductive Techniques ◽

Screening Tests ◽

Control Group ◽

Case Group ◽

Second Trimester ◽

Significant Difference ◽

Reproductive Techniques ◽

The Mean

Background: More than one million children throughout the world are born by using fertility techniques. In this process, human intervention and laboratory conditions may have an impact on the growth and development of the fetus. Objectives: The present study aimed to consider the effect of assisted reproductive techniques on the results of embryonic health screening tests. Methods: In this case-control study, among clients who went to the Infertility Center of the Ome Leila Hospital in Bandar Abbas Province, we selected 200 pregnant women who were impregnated via assisted reproductive techniques as the case group and 200 natural pregnant women as the control group by sample random sampling. Checklists were filled out and data analyzed by the SPSS version 21 and chi-square tests by using an interview with pregnant women and investigating the embryonic screening tests. Results: Most of the participants in the study were housewives and had primary infertility. Their BMI was normal. There was no significant difference between the final results of the embryonic screening test in the two groups at the first trimester (P > 0.05). However, in the second trimester, there was a significant difference between them. The positive results were higher in the case group in comparison with the control group (P = 0.001). The mean of all types of screening tests in the first trimester in both groups was not different, significantly (P < 0.05), although in the second trimester, the mean of all the testis, including BHCG (P = 0.006) and AFP (P = 0.018) in both case and control groups, was significantly different. The mean of BHCG and AFP was higher in the case group, while the mean of Estradiol and Inhibin, NB, and NT were not really different (P < 0.05). Conclusions: Our study showed that embryonic screening tests, particularly the BHCG and AFP tests, would be affected by applying the assisted reproductive techniques. For the couples who had a history of infertility and used reproductive methods, screening tests are essential in the first and second trimester.

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