scholarly journals Assessment of genetic diversity in five populations of Solanum torvum Swartz. from Tripura using SSR and RAPD markers

2020 ◽  
Vol 7 (1) ◽  
pp. 46-54
Author(s):  
Mitali Das ◽  
H R Singha ◽  
Kishan Saha
Keyword(s):  
Genetic Diversity ◽  
Polymerase Chain Reaction ◽  
High Altitude ◽  
Molecular Characterization ◽  
Rapd Markers ◽  
Common Ancestor ◽  
Chain Reaction ◽  
Solanum Torvum ◽  
Different Populations ◽  
Polymerase Chain

Polymerase Chain reaction (PCR) based molecular characterization has been undertaken for assessing the genetic diversity in five populations of Solanum torvum using SSR and RAPD markers. In this study, 8 SSRs produced 151 fragments of which 131 bands were polymorphic (86.38%). The primers, At5 amplified the highest number of polymorphic loci (27) and the highest PIC was recorded in CBT08 (0.54). In comparison, RAPD assay produced 70 bands with 79.16% polymorphism. The PIC value was highest in OPC14 (0.41). UPGMA clustering for SSR and RAPD markers grouped all the populations into two clusters. Our findings on SSR profile suggests that though different populations of S. torvum are inherited from a common ancestor eventually the population (STP5) with greater genetic diversity is stabilized in the high altitude of Sub - Himalayan region of Tripura in the due course of evolution.

scholarly journals Genetic diversity assessment using RAPD primers in insecticide resistant populations of diamondback moth Plutella xylostella (Linn.)

2015 ◽  
Vol 7 (1) ◽  
pp. 219-225 ◽  
Author(s):  
V. Sunitha ◽  
T.V. K. Singh ◽  
V. Ramesh Babu ◽  
J. Satyanarayana
Keyword(s):  
Genetic Diversity ◽  
Polymerase Chain Reaction ◽  
Genetic Variability ◽  
Plutella Xylostella ◽  
Rapd Markers ◽  
Diamondback Moth ◽  
Chain Reaction ◽  
Diversity Assessment ◽  
Polymerase Chain ◽  
Cluster A

Genetic diversity in acephate, spinosad and Cry2Ab resistant Plutella xylostella collected from three states of India was assessed by RAPD markers. The DNA extracted from larvae was subjected to polymerase chain reaction using 10 RAPD primers. The highest number alleles (7) were produced by primer ABA-13, followed by six alleles each by primers ABA-2, 7, 8, 11, 14; five alleles each were produced by ABA-4, 9, 10, 12. UPGMA analysis clustered the acephate, spinosad and Cry2Ab treated P.xylostella populations into two groups with overall similarity level of 33%, 27% and 34% respectively. Cluster A consisted 11 samples while Cluster B consisted only F1 of acephate and spinosad treated Karnataka population. In Cry2Ab treated population Cluster B comprised 11 samples and Cluster A had out grouped singly i.e. F0 generation from Karnataka. The genetic variability between the acephate, spinosad and Cry2Ab treated populations ranged from 33 to 69%, 27 to 56% and 34 to 69% respectively. Acephate and spinosad treated F1 population and Cry2Ab treated F0 population from Karnataka were out grouped from rest of the populations.

Molecular characterization of canine parvovirus in Brazil by polymerase chain reaction assay

2000 ◽  
Vol 75 (2) ◽  
pp. 127-133 ◽  
Author(s):  
Cesar A.D Pereira ◽  
Telma A Monezi ◽  
Dolores U Mehnert ◽  
Magali D’Angelo ◽  
Edison L Durigon
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Characterization ◽  
Polymerase Chain Reaction Assay ◽  
Canine Parvovirus ◽  
Chain Reaction ◽  
Polymerase Chain

Inheritance of random amplified polymorphic DNA markers in an interspecific cross in the genus Stylosanthes

Genome ◽  
1993 ◽  
Vol 36 (1) ◽  
pp. 50-56 ◽  
Author(s):  
Kemal Kazan ◽  
John M. Manners ◽  
Don F. Cameron
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Sequences ◽  
Rapd Markers ◽  
Dna Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Interspecific Cross ◽  
Parental Origin ◽  
Chain Reaction ◽  
Stylosanthes Hamata ◽  
Polymerase Chain

The inheritance of random amplified polymorphic DNA (RAPD) markers generated via the polymerase chain reaction amplification of genomic DNA sequences in an F2 family of an interspecific cross between Stylosanthes hamata and S. scabra was investigated. An initial comparison between the parental species, S. hamata cv. Verano and S. scabra cv. Fitzroy, demonstrated that 34% of detected RAPD bands were polymorphic. Of 90 primers tested, 35 showed relatively simple and reliably scorable polymorphisms and were used for segregation analysis. Sixty F2 individuals were scored for the segregation of 73 RAPD markers and 55 of these markers fit a 3:1 ratio. Segregation of eight other RAPD markers deviated significantly from a 3:1 ratio. There was no bias in the inheritance of RAPD markers regarding parental origin of the segregating RAPD markers. Linkage analysis revealed 10 linkage groups containing a total of 44 RAPD loci. Another 10 RAPD markers (7 of maternal origin) that were polymorphic between the parents did not segregate in the F2 population. One of the maternally inherited RAPD bands hybridized to chloroplast DNA. Analysis of RAPD loci by DNA hybridization indicated that mainly repeated sequences were amplified. These data indicate that RAPDs are useful genetic markers in Stylosanthes spp. and they may be suitable for genetic mapping.Key words: genetic mapping, molecular markers, polymerase chain reaction, Stylosanthes hamata, Stylosanthes scabra.

Molecular Characterization of Arcobacter Isolates Using Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR)

2014 ◽  
Vol 9 (9) ◽  
pp. 543-555 ◽  
Author(s):  
P.S. Bagalakote ◽  
R.S. Rathore ◽  
T.P. Ramees ◽  
H.V. Mohan ◽  
M. Sumankumar ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Characterization ◽  
Dna Polymerase ◽  
Randomly Amplified Polymorphic Dna ◽  
Chain Reaction ◽  
Rapd Pcr ◽  
Polymerase Chain

Rapid molecular characterization of Hb H disease in Chinese by polymerase chain reaction

1994 ◽  
Vol 68 (1) ◽  
pp. 33-37 ◽  
Author(s):  
J. G. Chang ◽  
T. C. Liu ◽  
L. I. Perng ◽  
S. S. Chiou ◽  
T. P. Chen ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Characterization ◽  
Chain Reaction ◽  
Hb H Disease ◽  
Polymerase Chain

scholarly journals The Molecular Characterization of Intestinal Explant HIV Infection Using Polymerase Chain Reaction-Based Techniques

2015 ◽  
Vol 31 (10) ◽  
pp. 981-991 ◽  
Author(s):  
Laura Janocko ◽  
Andrew D. Althouse ◽  
Rhonda M. Brand ◽  
Ross D. Cranston ◽  
Ian McGowan
Keyword(s):  
Polymerase Chain Reaction ◽  
Hiv Infection ◽  
Molecular Characterization ◽  
Chain Reaction ◽  
Polymerase Chain
Sign in / Sign up

Export Citation Format

Share Document