scholarly journals Comparative study of diagnostic methods used for monitoring of common grape vine (Vitis vinifera L.) crown gall (Agrobacterium vitis Ophel & Kerr) in Slovenia

2014 ◽  
Vol 103 (2) ◽  
pp. 313-321
Author(s):  
Janja LAMOVŠEK ◽  
Igor ZIDARIČ ◽  
Irena MAVRIČ PLEŠKO ◽  
Gregor UREK ◽  
Stanislav TRDAN
2015 ◽  
Vol 103 (2) ◽  
pp. 313 ◽  
Author(s):  
Janja LAMOVŠEK ◽  
Igor ZIDARIČ ◽  
Irena MAVRIČ PLEŠKO ◽  
Gregor UREK ◽  
Stanislav TRDAN

Agrobacterium vitis causes common grape vine (Vitis vinifera L.) crown gall disease that destroyed a lot of Slovenian vineyards more than a decade ago. Eighty isolates of Agrobacterium spp. collected during monitoring in 2006 were identified as A. vitis and A. tumefacies by pehA and multiplex PCR method. Tumor-inducing capacity of these strains was assessed on test plants and with PCR methods for detection of the Ti plasmid responsible for tumor induction. With VCF3/VCR3 primer pair six false negatives and no false positives were detected. The high genetic diversity of pathogenic Agrobacterium spp. strains affects the performance of molecular methods, thus biological test should be performed where results from molecular methods are doubtful.


2017 ◽  
Vol 16 (43) ◽  
pp. 2083-2091 ◽  
Author(s):  
Kinfe Beza ◽  
Feyssa Tileye ◽  
Bedada Girma

2019 ◽  
Vol 15 (2) ◽  
pp. 206-211
Author(s):  
С. І. Мельник ◽  
Н. С. Орленко ◽  
В. М. Матус ◽  
К. М. Мажуга ◽  
А. Н. Керімов

1983 ◽  
Vol 61 (10) ◽  
pp. 2641-2646 ◽  
Author(s):  
B. N. Dhanvantari

Crown gall was found mostly on vinifera grape (Vitis vinifera L.) cultivars in Ontario vineyards. The galls were usually small and aggregated along the trunk and cane; large woody galls were less common. Vines affected by crown gall died back in 3–4 years after planting. All the nine strains of Agrobacterium tumefaciens (Smith and Townsend) Conn isolated from galls corresponded to biotype 3 in determinative tests and were pathogenic on Datura, tobacco, tomato, and sunflower plants. Eight strains were sensitive to agrocin 84 on amended Stonier's medium. They were suppressed if inoculation was preceded 6 h earlier by Agrobacterium radiobacter strain K84 at wound sites on Datura and tomato plants.


2012 ◽  
Vol 194 (18) ◽  
pp. 5137-5138 ◽  
Author(s):  
Han Ming Gan ◽  
Teong Han Chew ◽  
André O. Hudson ◽  
Michael A. Savka

ABSTRACTNovosphingobiumsp. strain Rr 2-17 is anN-acyl homoserine lactone (AHL)-producing bacterium isolated from the crown gall tumor of a grapevine. To our knowledge, this is the first draft genome announcement of a plant-associated strain from the genusNovosphingobium.


1970 ◽  
Vol 23 (2) ◽  
pp. 479 ◽  
Author(s):  
MG Mullins ◽  
Daphne J Osborne

The inflorescences of woody grape vine cuttings usually atrophy if bud burst precedes emergence of adventitious roots (Mullins 1967). Roots are a site of cytokinin synthesis (Kende 1964; Itai and Vaadia 1965), and the ascending sap of vines is rich in endogenous cytokinin (Loeffler and Van Over beek 1964; Nitsch and Nitsch 1965; Skene and Kerridge 1967). The inflorescences of vine cuttings are retained and continue to grow if roots are initiated before bud burst, and the inflorescences of non-rooted cuttings are retained when treated with synthetic cytokinins (Mullins 1967).


2016 ◽  
Vol 16 (1) ◽  
Author(s):  
Céline Cholet ◽  
Stéphane Claverol ◽  
Olivier Claisse ◽  
Amélie Rabot ◽  
Audrey Osowsky ◽  
...  

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