Molecular identification of phytoplasmas associated with some weeds in West Azarbaijan province, Iran

During field surveys in 2013 and 2014, about 14 weed plants showing phytoplasma diseases symptoms including yellowing and witches’broom were collected and tested by polymerase chain reaction (PCR) using universal primers for 16SrRNA starting by primer pairs P1/P7 in first round PCR followed by primer pair R16F2n/R16R2 in nested PCR. The detected phytoplasmas were characterized and differentiated through sequence analysis of PCR-amplified rDNA and virtual restriction fragment length polymorphism (RFLP). The phytoplasmas detected in symptomatic horseweed (Erigeron canadensis L.), common madder (Rubia tinctorum L.), Johnson grass (Sorghum halepense [L.] Pers.) and Sophora root (Sophora alopecuroides L.) were identified as members of the clover proliferation group (16SrVI group) by construction of phylogenetic trees. Further analysis by virtual RFLP classified the phytoplasmas of Erigeron canadensis L. and Sorghum halepense L. in subgroup 16SrVI-A and phytoplasmas of Rubia tinctorum L. and Sophora alpecuriodes L. in subgroup 16SrVI-D. This is the first report on the occurrence of phytoplasma diseases of weeds in west Azarbaijan, Iran.

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Molecular identification of Candidatus Phytoplasma spp. associated with Sophora yellow stunt in Iran

Journal of Plant Protection Research ◽

10.1515/jppr-2017-0023 ◽

2017 ◽

Vol 57 (2) ◽

pp. 167-172 ◽

Cited By ~ 1

Author(s):

Touhid Allahverdi ◽

Heshmatollah Rahimian ◽

Mina Rastgou

Keyword(s):

Phylogenetic Trees ◽

Fragment Length Polymorphism ◽

Chain Reaction ◽

Mazandaran Province ◽

Nested Polymerase Chain Reaction ◽

Sophora Alopecuroides ◽

Leaf Yellowing ◽

Tehran Province ◽

Polymerase Chain ◽

West Azerbaijan

Abstract In the spring of 2012, sophora (Sophora alopecuroides L.) plants showing symptoms of leaf yellowing, little leaves and stunting were observed in Firooz-kuh (Tehran province), Sari (Mazandaran province) and Urmia (West Azerbaijan province) in Iran. Symptomatic plants from the three locations were subjected to nested polymerase chain reaction (PCR) to amplify 16SrRNA using primer pair P1/P7 followed by primer pair R16F2n/R16R2. The amplicons were purified, sequenced and the nucleotide sequences were analyzed by virtual restriction fragment length polymorphism (RFLP). The phytoplasmas associated with the yellows disease were identified as members of the 16SrIX group (Candidatus Phytoplasma phoenicium) and the 16SrXII group (Candidatus Phytoplasma solani). The two phytoplasmas were placed in 16SrIX-C and 16SrXII-A subgroups, respectively, in constructed phylogenetic trees. This is the first report on sophora yellows associated with Candidatus Phytoplasma phoenicium.

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Molecular Characterization and Classification of Phytoplasmas Associated with Canola Yellows and a New Phytoplasma Strain Associated with Dandelions

Plant Disease ◽

10.1094/pdis.2001.85.1.76 ◽

2001 ◽

Vol 85 (1) ◽

pp. 76-79 ◽

Cited By ~ 24

Author(s):

Keri Wang ◽

Chuji Hiruki

Keyword(s):

Fragment Length Polymorphism ◽

Rflp Analysis ◽

Universal Primers ◽

Dna Fragments ◽

Chain Reaction ◽

Aster Yellows ◽

Distinct Subgroup ◽

16S Ribosomal Dna ◽

Polymerase Chain

DNA isolated from symptomatic canola (Brassica napus, Brassica rapa) and dandelion (Taraxacum officinale) was used to amplify 16S ribosomal DNA fragments by polymerase chain reaction using two pairs of universal primers P1/P6 and R16F2n/R2. Restriction fragment length polymorphism (RFLP) analysis of the amplified DNA fragments using endonucleases AluI, HhaI, HpaII, MseI, RsaI, and Sau 3AI revealed two distinct types of phytoplasmas in canola with similar symptoms. One had the same RFLP profiles as the phytoplasmas in subgroup 16SrI-A, whereas the other one had RFLP profiles similar to those of phytoplasmas in subgroup 16SrI-B. Phytoplasmas were detected in symptomatic dandelion plants that were collected from canola and alfalfa fields where severe alfalfa witches'-broom occurred. Comparative studies indicated that two different phytoplasmas were associated with the dandelion plants. One was identified as a member of subgroup 16SrI-A, whereas another one was classified as a member of a distinct subgroup in the aster yellows group on the basis of the unique RFLP patterns.

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First Report of a Group 16SrII Phytoplasma Infecting Chickpea in Oman

Plant Disease ◽

10.1094/pd-90-0973c ◽

2006 ◽

Vol 90 (7) ◽

pp. 973-973 ◽

Cited By ~ 8

Author(s):

N. A. Al-Saady ◽

A. M. Al-Subhi ◽

A. Al-Nabhani ◽

A. J. Khan

Keyword(s):

Nested Pcr ◽

Animal Feed ◽

Restriction Enzymes ◽

Universal Primers ◽

Polymorphism Analysis ◽

Disease Symptoms ◽

First Report ◽

Pcr Products ◽

Polymerase Chain ◽

Group 2

Chickpea (Cicer arietinum), locally known as “Dungo”, is grown for legume and animal feed mainly in the interior region of Oman. During February 2006, survey samples of chickpea leaves from plants showing yellows disease symptoms that included phyllody and little leaf were collected from the Nizwa Region (175 km south of Muscat). Total nucleic acid was extracted from asymptomatic and symptomatic chickpea leaves using a cetyltrimethylammoniumbromide method with modifications (3). All leaf samples from eight symptomatic plants consistently tested positive using a polymerase chain reaction assay (PCR) with phytoplasma universal primers (P1/P7) that amplify a 1.8-kb phytoplasma rDNA product and followed by nested PCR with R16F2n/R16R2 primers yielding a product of 1.2 kb (2). No PCR products were evident when DNA extracted from healthy plants was used as template. Restriction fragment length polymorphism analysis of nested PCR products by separate digestion with Tru9I, HaeIII, HpaII, AluI, TaqI, HhaI, and RsaI restriction enzymes revealed that a phytoplasma belonging to group 16SrII peanut witches'-broom group (2) was associated with chickpea phyllody and little leaf disease in Oman. Restriction profiles of chickpea phytoplasma were identical with those of alfalfa witches'-broom phytoplasma, a known subgroup 16SrII-B strain (3). To our knowledge, this is the first report of phytoplasma infecting chickpea crops in Oman. References: (1) A. J. Khan et al. Phytopathology, 92:1038, 2002. (2). I.-M. Lee et al. Int. J. Syst. Bacteriol. 48:1153, 1998 (3) M. A. Saghai-Maroof et al. Proc. Natl. Acad. Sci. USA. 81:8014, 1984.

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Population variation in Sorghum halepense, Johnson grass, at the northern limits of its range

Canadian Journal of Botany ◽

10.1139/b84-242 ◽

1984 ◽

Vol 62 (9) ◽

pp. 1781-1790 ◽

Cited By ~ 31

Author(s):

S. I. Warwick ◽

B. K. Thompson ◽

L. D. Black

Keyword(s):

New York ◽

Dry Weight ◽

Population Variation ◽

Sorghum Halepense ◽

Johnson Grass ◽

Allocation Patterns ◽

Cultivated Species ◽

Relationship Of ◽

The Relationship ◽

Enzyme Patterns

Thirteen populations of Sorghum halepense, Johnson grass, were sampled from fields in Ontario, Canada, and Ohio and New York, United States. Only four of these populations were reported to overwinter as rhizomes. The morphology, phenology, resource allocation patterns, and growth of seedling and mature plants of the overwintering and the non-overwintering populations were compared. Field-collected specimens from the nonoverwintering populations had wider culms and leaves and larger seeds and inflorescences. Analysis of material grown in a 5-month greenhouse trial indicated similar differences. Greenhouse plants from the nonoverwintering populations were also characterized by greater percent emergence, larger and faster growing seedlings, earlier flowering, larger culms and seeds, greater reproductive dry weight per plant, and about 1/10th the rhizome dry weight of overwintering plants. Differences between populations within a biotype were evident for both biotypes, although there was little within-population variation, except in rhizome production, where certain individuals of some nonoverwintering populations did not produce extended rhizomes. Among the five enzymes which were examined electrophoretically, only one, phosphoglucomutase (PGM), showed variable isozyme patterns. No differences in enzyme patterns were apparent between the overwintering and the nonoverwintering biotypes. The relationship of the nonoverwintering populations to the cultivated species, Sorghum bicolor and S. almum, an introgressant between S. halepense and S. bicolor, is discussed.

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