A New Denoising Method for Time-lapse Video using Background Modeling

2020 ◽  
Vol 10 (2) ◽  
pp. 125-138
Author(s):  
Sanghyun Park
Keyword(s):  
Time Lapse ◽  
Background Modeling ◽  
Denoising Method ◽  
Time Lapse Video

Motion of Active Fluids: Diffusion Dynamics of Cyanobacteria

2016 ◽  
Author(s):  
Thomas Vourc’h ◽  
Julien Léopoldès ◽  
Annick Méjean ◽  
Hassan Peerhossaini
Keyword(s):  
Time Lapse ◽  
Global Behavior ◽  
Diffusion Dynamics ◽  
Mean Squared Displacement ◽  
Terrestrial Environments ◽  
The Mean ◽  
Micro Organisms ◽  
Synechocystis Sp ◽  
Time Lapse Video ◽  
Pcc 6803

Cyanobacteria are photosynthetic micro-organisms colonizing all aquatic and terrestrial environments. The motility of such living micro-organisms should make their diffusion distinct from typical Brownian motion. This diffusion can be investigated in terms of global behavior (Fickian or not) and in terms of displacement probabilities, which provide more detail about the motility process. Using cyanobacterium Synechocystis sp. PCC 6803 as the model micro-organism, we carry out time-lapse video microscopy to track and analyze the bacteria’s trajectories, from which we compute the mean-squared displacement (MSD) and the distribution function of displacement probabilities. We find that the motility of Synechocystis sp. PCC 6803 is intermittent: high-motility “run” phases are separated by low-motility “tumble” phases corresponding to trapped states. However, this intermittent motility leads to a Fickian diffusive behavior, as shown by the evolution of the MSD with time.

scholarly journals Salmonella stimulate macrophage macropinocytosis and persist within spacious phagosomes.

1994 ◽  
Vol 179 (2) ◽  
pp. 601-608 ◽  
Author(s):  
C M Alpuche-Aranda ◽  
E L Racoosin ◽  
J A Swanson ◽  
S I Miller
Keyword(s):  
Bone Marrow ◽  
Plasma Membrane ◽  
Time Lapse ◽  
Membrane Ruffling ◽  
Mouse Macrophages ◽  
Constitutive Mutation ◽  
Microscopic Studies ◽  
Specific Igg ◽  
Salmonella Survival ◽  
Time Lapse Video

Light microscopic studies of phagocytosis showed that Salmonella typhimurium entered mouse macrophages enclosed in spacious phagosomes (SP). Viewed by time-lapse video microscopy, bone marrow-derived macrophages exposed to S. typhimurium displayed generalized plasma membrane ruffling and macropinocytosis. Phagosomes containing Salmonella were morphologically indistinguishable from macropinosomes. SP formation was observed after several methods of bacterial opsonization, although bacteria opsonized with specific IgG appeared initially in small phagosomes that later enlarged. In contrast to macropinosomes induced by growth factors, which shrink completely within 15 min, SP persisted in the cytoplasm, enlarging often by fusion with macropinosomes or other SP. A Salmonella strain containing a constitutive mutation in the phoP virulence regulatory locus (PhoPc) induced significantly fewer SP. Similar to Yersinia enterocolitica, PhoPc bacteria entered macrophages in close-fitting phagosomes, consistent with that expected for conventional receptor-mediated phagocytosis. These results suggest that formation of SP contributes to Salmonella survival and virulence.

Collecting Vehicle-Speed Data by Using Time-Lapse Video Recording Equipment

2003 ◽  
Vol 1855 (1) ◽  
pp. 97-104 ◽  
Author(s):  
Christopher Strong ◽  
Scott Lowry ◽  
Peter McCarthy
Keyword(s):  
Real Time ◽  
Video Recording ◽  
Warning System ◽  
Time Lapse ◽  
Vehicle Speed ◽  
Recording Equipment ◽  
Safety Improvement ◽  
Camera Location ◽  
Message Signs ◽  
Time Lapse Video

An innovative application of time-lapse video recording is used to assist in an evaluation of a highway safety improvement. The improvement is an icy-curve warning system near Fredonyer Summit in northern California that activates real-time motorist warnings via extinguishable message signs, based on weather readings collected from road weather information systems. A measure of effectiveness is whether motorist speed is reduced as a result of real-time warnings to drivers. Why indirect speed measurement with video was preferred over radar for this case is discussed, as is how specific methodological issues related to the custom-built equipment, including camera location and orientation, distance benchmarking, and data collection and reduction. Theoretical and empirical accuracy measurements show that the video surveillance trailers yield results comparable to radar and, hence, would be applicable for studies in which speed change is measured. Because this particular technology had not been used previously, several lessons are documented that may help determine where and how similar equipment may be optimally used in future studies.

An automatic time lapse video system for optical microscopy

1991 ◽  
Vol 22 (3) ◽  
pp. 275-276
Author(s):  
B. Nys ◽  
A. Van Daele ◽  
W. Jacob
Keyword(s):  
Optical Microscopy ◽  
Time Lapse ◽  
Video System ◽  
Time Lapse Video

scholarly journals Time-lapse video observations on sexual plasmogamy in the red algaBostrychia

1998 ◽  
Vol 33 (1) ◽  
pp. 43-56 ◽  
Author(s):  
Jeremy Pickett-Heaps ◽  
John West
Keyword(s):  
Time Lapse ◽  
Video Observations ◽  
Time Lapse Video

An analysis of in vivo cell migration during teleost fin morphogenesis

1984 ◽  
Vol 66 (1) ◽  
pp. 205-222
Author(s):  
A. Wood ◽  
P. Thorogood
Keyword(s):  
Close Association ◽  
Video Recording ◽  
Time Lapse ◽  
Mesenchymal Cells ◽  
Mean Value ◽  
Distal Margin ◽  
Cellular Processes ◽  
Interference Contrast Microscopy ◽  
Time Lapse Video

In the teleost embryo the pectoral fin bud initially displays an apical ectodermal ridge along its entire distal margin. The ridge subsequently becomes transformed into an apical fold as the distal ectodermal epithelium grows and folds to enclose an extracellular space between the apposed basal surfaces of the epithelium. Collagen fibrils up to 2 micron in diameter, termed ‘actinotrichia’, are deposited along the proximo-distal axis in two (dorsal and ventral) arrays. The actinotrichia are aligned parallel to one another with a regular spacing along the greater part of their length. Mesenchymal cells migrating distally from the base of the fin bud encounter the dorsal and ventral arrays of actinotrichia and move between them apparently using the fibrils as a substratum. The entire structure is transparent and, using the killifish Aphyosemion scheeli, we have investigated the migration of the mesenchymal cells between 135 and 220 h of development, using Nomarski interference contrast microscopy and time-lapse video recording. The number of cellular processes per cell increased significantly during the period of observation. These processes could be graded according to their diameters. Processes of diameter greater than 2 micron were not usually aligned along actinotrichia and arose at any aspect of the cell body. In contrast, processes with diameters less than 2 micron appeared to be confined to the distal aspects of the migrating cells and showed an increasing tendency to become aligned as development progressed. Time-lapse video recordings revealed that such aligned processes move faster (mean speed 17.98 (+/− 2.25) micron/h) than non-aligned processes (mean speed 4.66 (+/− 0.67) micron/h). Whole cell translocation was generally slower than rates of process movement: the lowest mean value (1.52(+/− 0.36) micron/h) was recorded between 135 and 160 h of development rising to a maximum mean rate (4.72(+/− 0.42) micron/h) between 195 and 220 h; the period of the fastest rate of cell translocation correlated with maximum process alignment along actinotrichia. Thin 1 micron plastic sections revealed that, generally, aligned processes were in close association with the surface of the actinotrichial fibrils and not the spaces between them.

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