scholarly journals Use of agro-industrial residues for lipase production by Candida viswanathii in a solid-state cultivation system

2021 ◽  
Vol 17 (8) ◽  
Author(s):  
Alanna Cristinne Martins Lima ◽  
Camila Moitinho Dos Santos ◽  
Iara Leandro Dos Santos ◽  
Lunara Thaís Alves de Bastos ◽  
Annanda Carvalho dos Santos ◽  
...  
Keyword(s):  
Solid State ◽  
Olive Oil ◽  
Yeast Extract ◽  
Seed Coat ◽  
Scale Up ◽  
Soybean Seed ◽  
Lipase Production ◽  
Poultry Fat ◽  
Candida Viswanathii ◽  
Soybean Seed Coat

Lipases are an important group of enzymes that catalyze the hydrolysis of triacylglycerol and there are many industrial applications. The aim of this work was to produce the lipase by the yeast Candida viswanathii using solid state culture with agro-industrial wastes (barley bagasse, corn husk, corncob, soybean seed coat and soybean husk). The biomass pretreatment methods were evaluated, as well as the media supplementation with nitrogen and mixing substrates. Also, the efficiency of olive oil and poultry fat was evaluated on the induction of lipase production, followed by the scale-up from 20 g to 100 g. The enzyme activities in the cultures without pretreatement were higher when soybean seed coat supplemented with both olive oil (7.06 U/gss) and poultry fat (8.40 U/gss) were used. However, the pretreated substrates did not demonstrate a satisfying induction of lipolytic activity. From the nitrogen sources, yeast extract showed an increase of approximately twice the original production with both olive oil (18.12 U/gss) and poultry fat (15.98 U/gss) supplementation. On the scale-up step, the results demonstrated that, for the 20 g culture, the best lipase production was observed on the 7th day (33.52 U/gss), while for the 100 g culture the highest lipase activity was after 5 days (17.88 U/gss). The cultivation of ground soybean skin without pretreatment supplemented with yeast extract as a source of nitrogen, with fresh barley bagasse and poultry fat was the best combination.

scholarly journals Agroindustrial Wastes as Alternative for Lipase Production by Candida viswanathii under Solid-State Cultivation: Purification, Biochemical Properties, and Its Potential for Poultry Fat Hydrolysis

2016 ◽  
Vol 2016 ◽  
pp. 1-15 ◽  
Author(s):  
Alex Fernando de Almeida ◽  
Kleydiane Braga Dias ◽  
Ana Carolina Cerri da Silva ◽  
César Rafael Fanchini Terrasan ◽  
Sâmia Maria Tauk-Tornisielo ◽  
...  
Keyword(s):  
Solid State ◽  
Olive Oil ◽  
Initial Conditions ◽  
Lipase Production ◽  
Barley Grain ◽  
Hydrolysis Rate ◽  
Poultry Fat ◽  
Candida Viswanathii ◽  
Fat Hydrolysis ◽  
Agroindustrial Wastes

The aims of this work were to establish improved conditions for lipase production by Candida viswanathii using agroindustrial wastes in solid-state cultivation and to purify and evaluate the application of this enzyme for poultry fat hydrolysis. Mixed wheat bran plus spent barley grain (1 : 1, w/w) supplemented with 25.0% (w/w) olive oil increased the lipase production to 322.4%, compared to the initial conditions. When olive oil was replaced by poultry fat, the highest lipase production found at 40% (w/w) was 31.43 U/gds. By selecting, yeast extract supplementation (3.5%, w/w), cultivation temperature (30°C), and substrate moisture (40%, w/v), lipase production reached 157.33 U/gds. Lipase was purified by hydrophobic interaction chromatography, presenting a molecular weight of 18.5 kDa as determined by SDS-PAGE. The crude and purified enzyme showed optimum activity at pH 5.0 and 50°C and at pH 5.5 and 45°C, respectively. The estimated half-life at 50°C was of 23.5 h for crude lipase and 6.7 h at 40°C for purified lipase. Lipase presented high activity and stability in many organic solvents. Poultry fat hydrolysis was maximum at pH 4.0, reaching initial hydrolysis rate of 33.17 mmol/L/min. Thus, C. viswanathii lipase can be successfully produced by an economic and sustainable process and advantageously applied for poultry fat hydrolysis without an additional acidification step to recover the released fatty acids.

Development and characterization of a polyampholyte-based reactor immobilizing soybean seed coat peroxidase for analytical applications in a flow system

2011 ◽  
Vol 58-59 ◽  
pp. 57-68 ◽  
Author(s):  
Lisandro R. Denaday ◽  
M. Victoria Miranda ◽  
Rosa M. Torres Sánchez ◽  
Juan M. Lázaro Martínez ◽  
Lucía V. Lombardo Lupano ◽  
...  
Keyword(s):  
Seed Coat ◽  
Flow System ◽  
Soybean Seed ◽  
Analytical Applications ◽  
Soybean Seed Coat

scholarly journals Soybean Seed Coat Peroxidase (A Comparison of High-Activity and Low-Activity Genotypes)

1993 ◽  
Vol 103 (4) ◽  
pp. 1061-1066 ◽  
Author(s):  
M. Gijzen ◽  
R. van Huystee ◽  
R. I. Buzzell
Keyword(s):  
High Activity ◽  
Seed Coat ◽  
Soybean Seed ◽  
Soybean Seed Coat ◽  
Low Activity

Soybean seed coat chitinase as a defense protein against the stored product pestCallosobruchus maculatus

2018 ◽  
Vol 74 (6) ◽  
pp. 1449-1456 ◽  
Author(s):  
Nadia CM Silva ◽  
Jamile G Conceição ◽  
Kayan Eudorico Ventury ◽  
Leonardo FR De Sá ◽  
Eduardo AG Oliveira ◽  
...  
Keyword(s):  
Seed Coat ◽  
Soybean Seed ◽  
Defense Protein ◽  
Soybean Seed Coat

Soybean seed coat cup unloading on plants with low-raffinose, low-stachyose seeds

2009 ◽  
Vol 19 (3) ◽  
pp. 145-153 ◽  
Author(s):  
Suzanne M. Kosina ◽  
Alexander Castillo ◽  
Steven R. Schnebly ◽  
Ralph L. Obendorf
Keyword(s):  
Seed Coat ◽  
Soybean Seed ◽  
Soluble Carbohydrates ◽  
Growth Stages ◽  
In Planta ◽  
Plant Growth Stages ◽  
Soybean Plants ◽  
Soybean Seed Coat ◽  
Relationship Of ◽  
The Relationship

AbstractSucrose, raffinose and stachyose accumulate in soybean [Glycine max L. (Merrill)] embryos during seed maturation. To determine the relationship of plant maternal composition on seed composition, soluble carbohydrates in three 1-cm2 leaf punches at three plant growth stages (R2, R3, R6) and in seed coat cup exudates in planta were analysed at four 30-min intervals on soybean plants (R5) with low-raffinose, low-stachyose (LRS) seeds expressing the mutant stc1 phenotype; low-raffinose, low-stachyose and low-phytin (LRSP1, LRSP2) seeds expressing the mutant mips phenotype; or normal raffinose, stachyose and phytin (CHECK) seeds expressing the Stc1 and Mips phenotype. Leaf sucrose (23.6 μg cm− 2), myo-inositol (9.3 μg cm− 2), d-chiro-inositol (6.7 μg cm− 2), d-ononitol (0.76 μg cm− 2), d-pinitol (50.1 μg cm− 2) and total soluble carbohydrates (107.1 μg cm− 2) were not significantly different between phenotypes. d-chiro-Inositol, myo-inositol, d-pinitol and sucrose were unloaded from soybean seed coat cups in planta at decreasing rates over the four sequential periods of sampling. Unloading rates of sucrose and myo-inositol were highest for LRS, d-pinitol was highest for LRSP2, and d-chiro-inositol was not different between LRS, LRSP1, LRSP2 and CHECK. Free cyclitols were 60% of total soluble carbohydrates in leaves and 20% in seed coat cup exudates. Except for sucrose and d-pinitol, seed phenotype had little influence on the composition of compounds unloaded from seed coats to maturing embryos of low-raffinose, low-stachyose seeds. Maternally supplied cyclitols may contribute, in part, to changes in the composition of cyclitol galactosides stored in mature seeds.

Asymmetric glycosylation of soybean seed coat peroxidase

2006 ◽  
Vol 341 (2) ◽  
pp. 198-209 ◽  
Author(s):  
James S.S. Gray ◽  
Rex Montgomery
Keyword(s):  
Seed Coat ◽  
Soybean Seed ◽  
Soybean Seed Coat
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